Abstract:
:In adenovirus-infected cells, the epidermal growth factor receptor (EGF-R) is internalized from the cell surface via endosomes and is degraded, and the E3 10,400-dalton protein (10.4K protein) is required for this effect (C. R. Carlin, A. E. Tollefson, H. A. Brady, B. L. Hoffman, and W. S. M. Wold, Cell 57:135-144, 1989). We now report that both the E3 10.4K and E3 14.5K proteins are required for this down-regulation of EGF-R in adenovirus-infected cells. Down-regulation of cell surface EGF-R was demonstrated by results from several methods, namely the absence of EGF-R autophosphorylation in an immune complex kinase assay, the inability to iodinate EGF-R on the cell surface, the formation of endosomes containing EGF-R as detected by immunofluorescence, and the degradation of the metabolically [35S]Met-labeled fully processed 170K species of EGF-R. No effect on the initial synthesis of EGF-R was observed. This down-regulation was ascribed to the 10.4K and 14.5K proteins through the analysis of cells infected with rec700 (wild-type), dl748 (10.4K-, 14.5K+), or dl764 (10.4K+, 14.5K-) or coinfected with dl748 plus dl764. Further evidence that the 10.4K and 14.5K proteins function in concert was obtained by demonstrating that the 10.4K protein was coimmunoprecipitated with the 14.5K protein by using three different antisera to the 14.5K protein, strongly implying that the 10.4K and 14.5K proteins exist as a complex. Together, these results indicate that the 10.4K and 14.5K proteins function as a complex to stimulate endosome-mediated internalization and degradation of EGF-R in adenovirus-infected cells.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tollefson AE,Stewart AR,Yei SP,Saha SK,Wold WSdoi
10.1128/JVI.65.6.3095-3105.1991subject
Has Abstractpub_date
1991-06-01 00:00:00pages
3095-105issue
6eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
杂志文章abstract::Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced host cell apoptosis has been established, the underlying molecular mechanism is still unclear. We report here that IBDV viral protein 5 (VP5) is a major apoptosis induce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06104-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.4.776-784.1975
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pub_type: 杂志文章
doi:10.1128/JVI.79.18.12117-12121.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10458-10467.2000
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abstract::A rat nephroma cell line was found to be a carrier culture of the Kilham rat virus. Infectious virus was demonstrable only by passage of culture materials into other host systems. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.3.313-314.1969
更新日期:1969-09-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) alkaline nuclease, encoded by the UL12 gene, plays an important role in HSV-1 replication, as a UL12 null mutant displays a severe growth defect. The HSV-1 alkaline exonuclease UL12 interacts with the viral single-stranded DNA binding protein ICP8 and promotes strand exchange in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01506-10
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3751-3759.1997
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pub_type: 杂志文章
doi:10.1128/JVI.68.5.3154-3162.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.1.248-255.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
abstract::Group I host range (hr) mutants of adenovirus type 5 are unable to transform rat embryo or rat embryo brain cells but induce an abnormal transformation of baby rat kidney cells. We established several transformed rat kidney cell lines and characterized them with respect to the transformed phenotype and the structure o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.674-685.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8019-8026.1999
更新日期:1999-10-01 00:00:00
abstract::Epstein-Barr virus (EBV) efficiently drives proliferation of human primary B cells in vitro, a process relevant for human diseases such as infectious mononucleosis and posttransplant lymphoproliferative disease. Human B-cell proliferation is also driven by ligands of Toll-like receptors (TLRs), notably viral or bacter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01400-09
更新日期:2010-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.143-152.1979
更新日期:1979-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) clade C causes >50% of all HIV infections worldwide, and an estimated 90% of all transmissions occur mucosally with R5 strains. A pathogenic R5 simian-human immunodeficiency virus (SHIV) encoding HIV clade C env is highly desirable to evaluate candidate AIDS vaccines in nonh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00558-06
更新日期:2006-09-01 00:00:00
abstract::The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.1055-1062.2004
更新日期:2004-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.7056-7060.1991
更新日期:1991-12-01 00:00:00
abstract::The bovine leukemia virus (BLV) is an oncogenic retrovirus that is associated with the development of persistent lymphocytosis (PL) and lymphoma in cattle. While B lymphocytes have been shown to be the primary cellular target of BLV, recent studies suggest that some T lymphocytes and monocytes may be infected by the v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2178-2183.1996
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
abstract::In this paper, we report that the DNA of bovine mammillitis virus (BMV) consists of two covalently linked components that are 71.5 x 10(6) and 15.7 x 10(6) in molecular weight and designated L and S, respectively. We further report that the BMV DNA consists of four equimolar populations differing only in the orientati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.395-407.1978
更新日期:1978-01-01 00:00:00
abstract::The assembly of human cytomegalovirus (HCMV) is thought to be similar to that which has been proposed for alphaherpesviruses and involve envelopment of tegumented subviral particles at the nuclear membrane followed by export from the cell by a poorly defined pathway. However, several studies have shown that at least t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.975-986.2000
更新日期:2000-01-01 00:00:00
abstract::The lytic switch transactivator Zta initiates the ordered cascade of Epstein-Barr virus gene expression that culminates in virus production. Zta is a sequence-specific DNA-binding protein that transactivates early viral promotes via cis-acting sequences. Activation of some of these genes is mediated through binding to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4206-4212.1995
更新日期:1995-07-01 00:00:00
abstract::Glycosylated, membrane-associated E1 (58-kDa) and E2 (47- to 49-kDa) rubella virus proteins and unglycosylated nucleoprotein C (33 kDa), from separately expressed vaccinia virus recombinants, were injected into golden Syrian hamsters. Rubella virus E1 and E2 glycoproteins consistently induced an organ-specific autoimm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.1210-1214.1992
更新日期:1992-02-01 00:00:00
abstract::The small defective T particle of vesicular stomatitis virus (VSV-111) has no detectable enzyme activity when assayed at 31 C, although qualitatively it possesses all the virion proteins found in complete VSV-1 virions. With VSV-1 transcription product ribonucleic acid (RNA), it is shown that the VSV-111 RNA is identi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.6.946-955.1972
更新日期:1972-06-01 00:00:00
abstract:UNLABELLED:Hepatitis C virus (HCV) is a major cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma in humans. We showed previously that HCV induces autophagy for viral persistence by preventing the innate immune response. Knockdown of autophagy reduces extracellular HCV release, although the precis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02383-15
更新日期:2015-11-18 00:00:00
abstract::Closed circular unintegrated DNA of the SEATO strain of gibbon ape leukemia virus (GaLV-S) was isolated from canine thymus fibroblasts after cocultivation with chronically infected bat lung fibroblasts. Restriction endonuclease HindIII cleaves GaLV-S DNA once, thus allowing isolation and cloning of HindIII-digested un...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.1.269-275.1982
更新日期:1982-10-01 00:00:00
abstract::The first 86 residues of the Rous sarcoma virus (RSV) Gag protein form a membrane-binding (M) domain that directs Gag to the plasma membrane during budding. Unlike other retroviral Gag proteins, RSV Gag is not myristylated; however, the RSV M domain does contain 11 basic residues that could potentially interact with a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.23.11222-11229.2000
更新日期:2000-12-01 00:00:00
abstract::The helicase-like 1a and polymerase-like 2a proteins of brome mosaic virus (BMV) are required for viral RNA replication in vivo, are present in membrane-bound viral RNA polymerase extracts, and share conservation with the many other members of the alphavirus-like superfamily. To better understand BMV RNA replication a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8908-8916.1996
更新日期:1996-12-01 00:00:00