Abstract:
:The terminal 500-base-pair alpha sequence of the herpes simplex virus 1 genome contains signals for cleavage (Pac1 and Pac2) of unit-length DNA molecules from concatemers in unique stretches of sequences designated Ub and Uc, respectively, and a cis site for cleavage designated DR1. We report that nuclear extracts from infected cells contain factors which form two DNA-virus-specific protein complexes with components of the a sequence. Purification of the factors forming the V2 complex yielded a protein with an apparent molecular weight of 82,000 binding to DNA in a non-sequence-specific manner. Addition of Mg2+ to the purified protein-DNA probe mixture resulted in exonucleolytic degradation of the DNA. The protein was identified as the virus-specific DNase with monoclonal antibody specific for the viral enzyme. The purification of the proteins forming the V4 complex yielded two proteins with molecular weights of greater than 250,000 and 140,000 corresponding to infected cell protein 1 and to an as yet unidentified protein, respectively. These proteins formed two DNA sequence-common bands with a number of DNA probes and one sequence-specific band with probes containing both Pac2 and DR1 but not with probes containing either site alone or Pac1 and DR1. Since the DNA probe containing Pac2 and DR1 inserted into viral genome or into amplicons induced specific cleavage of the DR1 sequence whereas the nonreactive probes failed to induce the cleavage, the formation of this sequence-specific DNA-protein complex is significant and may reflect a DNA-protein interaction essential for cleavage. The possible role of the proteins identified in this study for the cleavage-packaging of viral DNA into capsids is presented.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chou J,Roizman Bdoi
10.1128/JVI.63.3.1059-1068.1989subject
Has Abstractpub_date
1989-03-01 00:00:00pages
1059-68issue
3eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
杂志文章abstract:UNLABELLED:Children with acute respiratory syncytial virus (RSV) infection often develop sequelae of persistent airway inflammation and wheezing. Pulmonary C fibers (PCFs) are involved in the generation of airway inflammation and resistance; however, their role in persistent airway diseases after RSV is unexplored. Her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02534-15
更新日期:2015-12-16 00:00:00
abstract::Growing CV1 cells were infected with simian virus 40 (SV40), and the levels of DNA polymerases-alpha, -beta, and -gamma were analyzed in the cytoplasm, nuclear Triton wash, and nucleus. In the cytoplasmic fraction, the amount of alpha-, beta-, or gamma-polymerase remained unaltered after SV40 infection. The activity o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.1.117-125.1977
更新日期:1977-07-01 00:00:00
abstract::Incorporation of the vaccinia virus A36R protein into the outer membrane of intracellular enveloped virions (IEV) is dependent on expression of the A33R protein. Possible interactions of the 200-amino-acid cytoplasmic domain of the A36R protein with itself or with the cytoplasmic domain of the A33R, A34R, B5R, or F12L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.7.4113-4126.2003
更新日期:2003-04-01 00:00:00
abstract::The synthesis of membrane protein after infection with bacteriophage T4 was examined. Protein constituents of both the cytoplasmic and outer membrane are made during the infective cycle. In addition, newly synthesized membrane protein is found in material which has a buoyant density greater than that of either of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.73-80.1974
更新日期:1974-01-01 00:00:00
abstract::Lack of disease in long-term nonprogressors with human immunodeficiency virus type 1 (HIV-1) infection was strongly associated with very low copy numbers of HIV-1 DNA and RNA in peripheral blood mononuclear cells and plasma and the presence of high levels of anti-HIV-1 CD8+ memory cytotoxic T lymphocytes specific for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5838-5842.1995
更新日期:1995-09-01 00:00:00
abstract::Walleye dermal sarcoma (WDS) and walleye epidermal hyperplasia (WEH) are skin diseases of walleye fish that appear and regress on a seasonal basis. We report here that the complex retroviruses etiologically associated with WDS (WDS virus [WDSV]) and WEH (WEH viruses 1 and 2 [WEHV1 and WEHV2, respectively]) encode D-ty...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8765-8771.1998
更新日期:1998-11-01 00:00:00
abstract::Tumor necrosis factor alpha (TNF-alpha) induced morphologic changes such as chromatin condensation and cell shrinkage in a feline fibroblastic cell line (CRFK) chronically infected with feline immunodeficiency virus (FIV) but not in uninfected CRFK cells. DNA extracted from TNF-alpha-treated CRFK cells infected with F...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2429-2433.1993
更新日期:1993-05-01 00:00:00
abstract::In all retroviruses analyzed to date (except for the spumaretroviruses), the Zn(2+)-coordinating residues of nucleocapsid (NC) perform or assist in crucial reactions necessary to complete the retrovirus life cycle. Six replication-defective mutations have been engineered in the two NC Zn(2+) fingers (ZFs) of simian im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.115-124.2001
更新日期:2001-01-01 00:00:00
abstract::Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have examined the prM/M and a portion of the E protein for a panel of 38 wild strains of YF virus from Africa representing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6999-7008.2001
更新日期:2001-08-01 00:00:00
abstract::To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.739-747.1984
更新日期:1984-06-01 00:00:00
abstract::The physical map locations of 62 temperature-sensitive mutations of vaccinia virus WR have been determined by marker rescue experiments, using cloned HindIII fragments of wild-type DNA. Since vaccinia virus DNA is not infectious, marker rescue was performed by infecting monolayers of cells at the nonpermissive tempera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.2.419-428.1983
更新日期:1983-11-01 00:00:00
abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4564-4570.1997
更新日期:1997-06-01 00:00:00
abstract::In contrast to humans, adult but not infant small animals are resistant to rotavirus diarrhea. The pathophysiological mechanism behind this age-restricted diarrhea is currently unresolved, and this question was investigated by studying the secretory state of the small intestines of adult mice infected with rotavirus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00152-06
更新日期:2006-11-01 00:00:00
abstract::Analyses of the breadth and specificity of virus-specific CD8(+) T cell responses associated with control of HIV have largely relied on measurement of cytokine secretion by effector T cells. These have resulted in the identification of HIV elite controllers with low or absent responses in which non-T-cell mechanisms o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00531-12
更新日期:2012-06-01 00:00:00
abstract::Open reading frame 24 (ORF24) of murine gammaherpesvirus 68 (MHV-68) is conserved among beta- and gammaherpesviruses; however, its function in viral replication has not been defined. Using MHV-68 as a model, we have identified ORF24 as being essential for viral replication. An ORF24-null virus was generated and shown ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02726-06
更新日期:2007-06-01 00:00:00
abstract::Rabies, as the oldest known infectious disease, remains a serious threat to public health worldwide. The eukaryotic cytosolic chaperonin TRiC/CCT complex facilitates the folding of proteins through ATP hydrolysis. Here, we investigated the expression, cellular localization, and function of neuronal CCTγ during neurotr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03186-12
更新日期:2013-07-01 00:00:00
abstract::Growth kinetics in lymphocytic H9 and M8166 cells of two mutants of human immunodeficiency virus type 1 (HIV-1) with deleted gp41 cytoplasmic tails were examined. While the mutant viruses designated CTdel-44 and CTdel-144 were able to grow in M8166 cells, they were unable to grow in H9 cells. Transfection and single-r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4891-4893.2000
更新日期:2000-05-01 00:00:00
abstract::Gammaherpesviruses are important human and animal pathogens. Infection control has proven difficult because the key process of transmission is ill understood. Murid herpesvirus 4 (MuHV-4), a gammaherpesvirus of mice, is transmitted sexually. We show that this depends on the major virion envelope glycoprotein gp150. gp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00235-17
更新日期:2017-06-09 00:00:00
abstract::We previously reported the establishment of several lymphoid cell lines from X-ray-induced thymomas of C57BL/Ka mice, and all, except one, produce retroviruses (P. Sankar-Mistry and P. Jolicoeur, J. Virol.35:270-275, 1980). Biological characterization of five of these new primary radiation leukemia viruses (RadLVs) in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.45.2.565-575.1983
更新日期:1983-02-01 00:00:00
abstract::Some paramyxovirus V proteins induce STAT protein degradation, and the amino acids essential for this process in the human parainfluenza virus type 2 (hPIV2) V protein have been studied. Various recombinant hPIV2s and cell lines constitutively expressing various mutant V proteins were generated. We found that V protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8591-8601.2005
更新日期:2005-07-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into HIV-1 particles. Rab11-family interacting protein 1C (FIP1C) and Rab14 are host trafficking factors required for Env particle incorpora...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01893-17
更新日期:2018-02-12 00:00:00
abstract::We have investigated the species-specific replication of polyomavirus DNA in the cell-free system that was established previously (Y. Murakami, T. Eki, M. Yamada, C. Prives, and J. Hurwitz, Proc. Natl. Acad. Sci. USA 83:6347-6351, 1986). Extracts from various species of cells supported polyomavirus DNA replication in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.9.4874-4881.1991
更新日期:1991-09-01 00:00:00
abstract::The M2-1 protein of respiratory syncytial (RS) virus is a transcriptional processivity and antitermination factor. The M2-1 protein has a Cys3His1 zinc binding motif which is essential for function, is phosphorylated, and has been shown to interact with the RS virus nucleocapsid (N) protein. In the work reported here,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.12188-12197.2001
更新日期:2001-12-01 00:00:00
abstract::HIV protease is known to cause cell death, which is dependent upon cleavage of procaspase 8. HIV protease cleavage of procaspase 8 generates Casp8p41, which directly binds Bak with nanomolar affinity, causing Bak activation and consequent cell death. Casp8p41 can also bind Bcl2 with nanomolar affinity, in which case c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00037-18
更新日期:2018-06-13 00:00:00
abstract::The establishment of de novo-generated patterns of DNA methylation is characterized by the gradual spreading of DNA methylation (I. Kuhlmann and W. Doerfler, J. Virol. 47:631-636, 1983; M. Toth, U. Lichtenberg, and W. Doerfler, Proc. Natl. Acad. Sci. USA 86:3728-3732, 1989; M. Toth, U. Müller, and W. Doerfler J. Mol. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4301-4308.1991
更新日期:1991-08-01 00:00:00
abstract::Epstein-Barr virus (EBV) in vivo is known to establish persistent infection in resting, circulating memory B cells and to productively replicate in plasma cells. Until now, the molecular mechanism of how EBV switches from latency to lytic replication in vivo was not known. Here, we report that the plasma cell differen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01055-07
更新日期:2007-12-01 00:00:00
abstract::The 3C region of human rhinovirus 14 was expressed in Escherichia coli. The microbially synthesized protease was functional, since the expressed precursor underwent autoproteolytic processing to generate mature molecules of the expected molecular weight and antigenicity. Mutation of the putative active-site Cys-146 re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5037-5045.1989
更新日期:1989-12-01 00:00:00
abstract::The monoclonal antibody PAb280 binds to small t antigen but not to large T antigen. Its binding site within the unique region of small t antigen was localized by studying its reaction with simian virus 40 mutants, other papovaviruses, and bacterial expression vectors coding for fragments of small t antigen. The antibo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3128-3134.1989
更新日期:1989-07-01 00:00:00
abstract::The sequence of the human immunodeficiency virus type 2 circle junction was determined. The most common sequence found between the conserved CA and TG dinucleotides at the ends of the integrated provirus was five bases long (GGTAC). This suggests that the integration of human immunodeficiency virus type 2 DNA is accom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3906-3910.1991
更新日期:1991-07-01 00:00:00
abstract::The hepatitis C virus (HCV) core gene is more conserved at the nucleic acid level than is necessary to preserve the sequence of the core protein, suggesting that it contains information for additional functions. We used a battery of anticore antibodies to test the hypothesis that the core gene directs the synthesis of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01679-08
更新日期:2009-04-01 00:00:00