Abstract:
:The 3C region of human rhinovirus 14 was expressed in Escherichia coli. The microbially synthesized protease was functional, since the expressed precursor underwent autoproteolytic processing to generate mature molecules of the expected molecular weight and antigenicity. Mutation of the putative active-site Cys-146 residue to an alanine resulted in the synthesis of unprocessed precursor molecules. Large quantities of the 20-kilodalton protease were purified by a simple purification protocol, and the resulting molecule was shown to be biologically active in vitro against synthetic peptides corresponding to the 2C-3A cleavage site. This site was cleaved with high efficiency and fidelity and was used to generate kinetic data on the 3C protease. The protease exhibited sensitivity to Zn2+, was capable of cleaving five of seven rhinovirus cleavage site peptides tested with variable efficiency, and could distinguish authentic substrate peptides from control peptides containing the dipeptide cleavage sequence pair Gln-Gly.
journal_name
J Viroljournal_title
Journal of virologyauthors
Cordingley MG,Register RB,Callahan PL,Garsky VM,Colonno RJdoi
10.1128/JVI.63.12.5037-5045.1989subject
Has Abstractpub_date
1989-12-01 00:00:00pages
5037-45issue
12eissn
0022-538Xissn
1098-5514journal_volume
63pub_type
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pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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journal_title:Journal of virology
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