Abstract:
:To study the expression of the Autographa californica nuclear polyhedrosis virus (AcNPV) genome, intracellular virus-specific proteins and mRNAs were pulsed-labeled, extracted, and analyzed at 6-h intervals during the replicative cycle. Most RNAs were detected between 12 and 24 h postinfection (p.i.), but many continued to be synthesized until late in infection. Polyhedrin and p8 mRNAs were the two most abundant late viral RNA transcripts, and they were synthesized at high rates until late in the infection cycle (60 h p.i.). The abundancy control of polyhedrin and p8 polypeptides was considered to be at the level of transcription. Two other major mRNAs in infected cells were 0.6-kilobase RNA, which was synthesized at its highest rate 12 to 18 h p.i., and 2.8-kilobase RNA, which was synthesized from 12 h p.i. until 48 h p.i. Cytoplasmic polyadenylic acid-containing RNA was isolated at 6-h intervals and was analyzed by Northern blot hybridization. At least 50 virus RNA transcripts were recognized, sized, and mapped onto the genome. Six RNAs hybridized to EcoRI-H, -I, and -J, and HindIII-Q AcNPV DNA restriction fragments, seven RNAs hybridized to EcoRI-B and -D DNA fragments, five RNAs hybridized to EcoRI-A and -E regions of the genome, four RNAs hybridized to EcoRI-C and -N DNA fragments, and one RNA species hybridized to EcoRI-O AcNPV DNA. A transcription map of the AcNPV genome was constructed, and the data were correlated with previously published translation maps.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rohel DZ,Faulkner Pdoi
10.1128/JVI.50.3.739-747.1984subject
Has Abstractpub_date
1984-06-01 00:00:00pages
739-47issue
3eissn
0022-538Xissn
1098-5514journal_volume
50pub_type
杂志文章abstract::The role of the cell nucleus in the development of African swine fever virus in Vero cells has been studied. No viral growth could be detected in enucleated cells under conditions that allow normal development of Sindbis virus. Furthermore, African swine fever virus DNA synthesis was inhibited more than 95% after infe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.902-905.1977
更新日期:1977-03-01 00:00:00
abstract::The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of alphabaculoviruses, is an enveloped DNA virus that infects lepidopteran insects and is commonly known as a vector for protein expression and cell transduction. AcMNPV belongs to a diverse group of viral and bacterial pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-18
更新日期:2018-05-14 00:00:00
abstract::Adeno-associated virus type 5 (AAV5) has a linear, single-stranded DNA genome of ca. 5 kb and an overlapping transcription profile featuring multiple promoters and a single intron in the center of the genome. Unlike the situation for the prototype AAV2, AAV5 RNAs transcribed from upstream promoters at map units 7 (P7)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.83-93.2004
更新日期:2004-01-01 00:00:00
abstract::The papillomavirus (PV) E2 protein is a critical regulator of viral transcription and genome replication. We previously reported that tyrosine (Y) 138 of HPV-31 E2 is phosphorylated by the fibroblast growth factor receptor 3 (FGFR3) kinase. In this study, we generated quasiviruses containing G418-selectable HPV-31 gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-20
更新日期:2020-07-01 00:00:00
abstract::The 86-kDa IE-2 protein of human cytomegalovirus is able to autoregulate its own expression via a short nucleotide sequence, termed the cis repression signal (CRS), that is located between the TATA box and the cap site of the IE-1/2 enhancer-promoter. Here we report that the 86-kDa IE-2 protein can interact directly w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.323-331.1993
更新日期:1993-01-01 00:00:00
abstract::Varicella-zoster virus (VZV) encodes within its unique long region a gene product with protein kinase motifs. In a previous study, we demonstrated that immunoprecipitated VZV open reading frame (ORF) 47 protein was associated with a functional protein kinase activity, on the basis of its ability to both autophosphoryl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1350-1359.1994
更新日期:1994-03-01 00:00:00
abstract::Aichi virus, a member of the family Picornaviridae, encodes a leader (L) protein of 170 amino acids (aa). The Aichi virus L protein exhibits no significant sequence homology to those of other picornaviruses. In this study, we investigated the function of the Aichi virus L protein in virus growth. In vitro translation ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.10799-10807.2003
更新日期:2003-10-01 00:00:00
abstract:UNLABELLED:HIV-1 Vpu decreases the exposure of epitopes within the viral envelope glycoprotein (Env) on the surface of infected cells by downregulating both BST2 and CD4. To test the hypothesis that inhibiting Vpu activity would increase the exposure of these epitopes and sensitize infected cells to antibody-dependent ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02736-15
更新日期:2015-12-16 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) accessory protein Vpu enhances virus particle release by counteracting a host factor that retains virions at the surfaces of infected cells. It was recently demonstrated that cellular protein BST-2/CD317/Tetherin restricts HIV-1 release in a Vpu-dependent manner. Calcium...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01786-09
更新日期:2009-12-01 00:00:00
abstract::Expression of mouse mammary tumor virus (MMTV)-encoded superantigens in B lymphocytes is required for viral transmission and pathogenesis. We have previously established a critical role of an enhancer element within the long terminal repeat (LTR) for MMTV sag gene expression in B-lymphoid progenitor cells. We now demo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.8183-8187.2000
更新日期:2000-09-01 00:00:00
abstract::Astrovirus is one of the major causes of infant and childhood diarrhea worldwide. Our understanding of astrovirus pathogenesis trails behind our knowledge of its molecular and epidemiologic properties. Using a recently developed small-animal model, we investigated the mechanisms by which astrovirus induces diarrhea an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1564-1574.2004
更新日期:2004-02-01 00:00:00
abstract::The hemagglutinin-neuraminidase (HN) glycoprotein plays a critical role in parainfluenza virus replication. We recently found that in addition to the catalytic binding site, HN of human parainfluenza virus type 1 (hPIV-1) may have a second receptor-binding site covered by an N-linked glycan at residue 173, which is ne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02331-09
更新日期:2010-03-01 00:00:00
abstract::The role of the influenza A virus panhandle structure formed from the 3'- and 5'-terminal nucleotides of virion RNA segments was studied in both an RNA polymerase binding assay and an in vitro transcription assay. Despite recent indications that promoter activity is simply a function of the 3'-terminal sequence of vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.6.4092-4096.1994
更新日期:1994-06-01 00:00:00
abstract::The ability of B cells, B blasts, and macrophages to present Epstein-Barr virion antigens to autologous T cells and trigger their capacity to inhibit Epstein-Barr virus-induced B-cell transformation was tested. Macrophages were as efficient as B cells and B blasts in presenting the virus to T lymphocytes. This functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1398-1401.1990
更新日期:1990-03-01 00:00:00
abstract::Envelope glycoprotein (Env) reactivity (ER) describes the propensity of human immunodeficiency virus type 1 (HIV-1) Env to change conformation from the metastable unliganded state in response to the binding of ligands (antibodies and soluble CD4 [sCD4]) or incubation in the cold. To investigate Env properties that fav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01558-13
更新日期:2014-01-01 00:00:00
abstract::The St-1 genome is about 6,050 base pairs in size, approximately 10% larger than phiX174 (5,375 base pairs). The DNA fragments obtained by HincII, HaeIII, and EcoRI digestion were ordered and aligned into a colinear map, and the single BglI cleavage site was located. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.3.738-744.1978
更新日期:1978-09-01 00:00:00
abstract::Under natural conditions and in some experimental models, rabies virus infection of the central nervous system causes relatively mild histopathological changes, without prominent evidence of neuronal death despite its lethality. In this study, the effects of rabies virus infection on the structure of neurons were inve...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01677-07
更新日期:2008-01-01 00:00:00
abstract::Epstein-Barr virus (EBV) in vivo is known to establish persistent infection in resting, circulating memory B cells and to productively replicate in plasma cells. Until now, the molecular mechanism of how EBV switches from latency to lytic replication in vivo was not known. Here, we report that the plasma cell differen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01055-07
更新日期:2007-12-01 00:00:00
abstract::Retroviral recombination can occur between two viral RNA molecules (intermolecular) or between two sequences within the same RNA molecule (intramolecular). The rate of retroviral intramolecular recombination is high. Previous studies showed that, after a single round of replication, 50 to 60% of retroviral recombinati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.9614-9623.2002
更新日期:2002-10-01 00:00:00
abstract::Recombinant poxvirus vectors are undergoing intensive evaluation as vaccine candidates for a variety of infectious pathogens. Avipoxviruses, such as canarypox virus, are replication deficient in mammalian cells by virtue of a poorly understood species-specific restriction. Highly attenuated vaccinia virus strains such...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02654-06
更新日期:2007-07-01 00:00:00
abstract::Understanding the mechanism(s) of action of the hepatitis B virus (HBV)-encoded protein HBx is fundamental to elucidating the underlying mechanisms of chronic liver disease and hepatocellular carcinoma caused by HBV infection. In our continued attempts to identify cellular targets of HBx, we have previously reported t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2840-2846.2000
更新日期:2000-03-01 00:00:00
abstract::The simian-human immunodeficiency virus SHIV-HXBc2 contains the envelope glycoproteins of a laboratory-adapted, neutralization-sensitive human immunodeficiency virus type 1 variant, HXBc2. Serial in vivo passage of the nonpathogenic SHIV-HXBc2 generated SHIV KU-1, which causes rapid CD4(+) T-cell depletion and an AIDS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4208-4218.2001
更新日期:2001-05-01 00:00:00
abstract::Kaposi sarcoma-associated herpesvirus (KSHV) is an emerging pathogen and is the causative infectious agent of Kaposi sarcoma and two malignancies of B cell origin. To date, there is no licensed KSHV vaccine. Development of an effective vaccine against KSHV continues to be limited by a poor understanding of how the vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00630-19
更新日期:2019-07-30 00:00:00
abstract::Human cytomegalovirus (HCMV) encodes four putative G protein-coupled receptors, including pUL78, whose rodent orthologues are known to be important for replication and spread in their hosts. To investigate the mechanism by which pUL78 contributes to viral replication and pathogenesis, we generated a derivative of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05900-11
更新日期:2012-11-01 00:00:00
abstract::During primary infection, the number of HIV-1 particles in plasma increases rapidly, reaches a peak, and then declines until it reaches a set point level. Understanding the kinetics of primary infection, and its effect on the establishment of chronic infection, is important in defining the early pathogenesis of HIV. W...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00127-10
更新日期:2010-06-01 00:00:00
abstract::A selection strategy, the activator trap, was used in order to identify genes of human cytomegalovirus (HCMV) that encode strong transcriptional activation domains in mammalian cells. This approach is based on the isolation of activation domains from a GAL4 fusion library by means of selective plasmid replication, whi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4836-4847.2002
更新日期:2002-05-01 00:00:00
abstract::Several arenaviruses, chiefly Lassa virus (LASV) and Junin virus in West Africa and Argentina, respectively, cause hemorrhagic fever (HF) disease in humans that is associated with high morbidity and significant mortality. The investigation of antiviral strategies to combat HF arenaviruses is hampered by the requiremen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02229-10
更新日期:2011-02-01 00:00:00
abstract::To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.65-70.1986
更新日期:1986-01-01 00:00:00
abstract::Proteins specific for human herpesvirus 6 (HHV-6)-infected human T cells (HSB-2) were examined by using polyclonal rabbit antibodies and monoclonal antibodies against HHV-6-infected cells and human sera. More than 20 proteins and six glycoproteins specific for HHV-6-infected cells were identified from [35S]methionine-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2835-2840.1989
更新日期:1989-06-01 00:00:00
abstract::The nef genes of the human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2) and the related simian immunodeficiency viruses (SIVs) encode a protein (Nef) whose role in virus replication and cytopathicity remains uncertain. As an attempt to elucidate the function of nef, we characterized the nucleotide and corre...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4639-4650.1993
更新日期:1993-08-01 00:00:00