Abstract:
:To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found whose dual phenotypes were the result of a single or two closely linked mutations. Genetic analyses of the alpha rts mutant library revealed two mutants, alpha rts7 and alpha rts12, that apparently fit this criterion; in alpha rts7 the two lesions were indistinguishable, whereas in alpha rts12 the two mutations were closely linked but separable. Cloned vaccinia virus HindIII DNA fragments were used to marker rescue the temperature-sensitive phenotype of these two dual mutants. The temperature-sensitive lesion of alpha rts7 was rescued by the HindIII N fragment (1.5 kilobases), whereas alpha rts12 was rescued by the neighboring HindIII M fragment (2.0 kilobases). The progeny virions of the alpha rts7 HindIII-N rescue reverted to an alpha-amanitin-sensitive phenotype, whereas the alpha rts12 HindIII-M progeny were still resistant to the drug. Taken together, these data indicate that the gene encoding alpha-amanitin resistance maps to the HindIII N fragment and provides evidence for the existence of essential vaccinia virus genes in a region of the genome previously believed to be nonessential for replication in tissue culture. Biochemical analyses revealed that both mutants were capable of synthesizing DNA as well as early and late viral proteins at the permissive and nonpermissive temperatures. At the nonpermissive temperature alpha rts12 and alpha rts7 were unable to process the major core precursors P94 and P65 into VP62 and VP60.
journal_name
J Viroljournal_title
Journal of virologyauthors
Villarreal EC,Hruby DEdoi
10.1128/JVI.57.1.65-70.1986subject
Has Abstractpub_date
1986-01-01 00:00:00pages
65-70issue
1eissn
0022-538Xissn
1098-5514journal_volume
57pub_type
杂志文章abstract::A recent publication indicated that overexpression of Axl, a cellular receptor that negatively regulates Toll-like receptor signaling, enhanced the entry of viruses pseudotyped with the glycoprotein of lymphocytic choriomeningitis virus (LCMV) in vitro. In testing the biological relevance of these observations, we fou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03268-12
更新日期:2013-04-01 00:00:00
abstract::Sequences from the 5' end of type 1 human immunodeficiency virus RNA dimerize spontaneously in vitro in a reaction thought to mimic the initial step of genomic dimerization in vivo. Dimer initiation has been proposed to occur through a "kissing-loop" interaction involving a specific RNA stem-loop element designated SL...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5902-5908.1996
更新日期:1996-09-01 00:00:00
abstract::A gene encoding a homolog of glycoprotein B of herpes simplex virus (gB homolog) has been identified on the Marek's disease virus (MDV) genome (L. J. N. Ross, M. Sanderson, S. D. Scott, M. M. Binns, T. Doel, and B. Milne, J. Gen. Virol. 70:1789-1804, 1989); however, the molecular and immunological characteristics of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2631-2638.1992
更新日期:1992-05-01 00:00:00
abstract::After corneal inoculation, herpes simplex virus type 1 replicates in the mouse eye, trigeminal ganglia, and brainstem, producing first an acute and then a latent infection. Previous work from this laboratory focused on the structure of the viral DNA in this system. We have now examined the structure of the viral genom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.3.764-767.1986
更新日期:1986-09-01 00:00:00
abstract::Developing an immunotherapy to keep human immunodeficiency virus type 1 (HIV-1) replication suppressed while discontinuing highly active antiretroviral therapy (HAART) is an important challenge. In the present work, we evaluated in vitro whether dendritic cells (DC) electroporated with gag mRNA can induce HIV-specific...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-07
更新日期:2008-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03459-12
更新日期:2013-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3145-3153.1994
更新日期:1994-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.6973-6978.1993
更新日期:1993-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.951-957.1979
更新日期:1979-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02962-14
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.4.2.117-122.1969
更新日期:1969-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00404-09
更新日期:2009-08-01 00:00:00
abstract::Primary RNA transcripts from the human immunodeficiency virus type 1 (HIV-1) are processed into mature mRNA by a complex series of splicing events. Viral structural proteins and reverse transcriptase are translated from unspliced or singly spliced transcripts. Proteins which control virus replication, including tat, r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3391-3398.1990
更新日期:1990-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06636-11
更新日期:2012-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.10.5735-5743.1992
更新日期:1992-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02352-10
更新日期:2011-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.708-716.1984
更新日期:1984-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4133-4141.1993
更新日期:1993-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3344-3348.1991
更新日期:1991-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8613-8619.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.20.11125-11138.2003
更新日期:2003-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.374-381.1976
更新日期:1976-08-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection is a major cause of liver disease. The HCV polyprotein contains a hypervariable region (HVR1) located at the N terminus of the second envelope glycoprotein E2. The strong variability of this 27-amino-acid region is due to its apparent tolerance of amino acid substitutions toge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5703-5710.2001
更新日期:2001-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2247-2250.2003
更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.72.6.5256-5261.1998
更新日期:1998-06-01 00:00:00