Abstract:
:Viral infection triggers host innate immune responses through cellular sensor molecules which activate multiple signaling cascades that induce the production of interferons (IFN) and other cytokines. The recent identification of mammalian cytoplasmic viral RNA sensors, such as retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and their mitochondrial adaptor, the mitochondrial antiviral signaling protein (MAVS), also called IPS-1, VISA, and Cardif, highlights the significance of these molecules in the induction of IFN. Teleost fish also possess a strong IFN system, but nothing is known concerning the RLRs and their downstream adaptor. In this study, we cloned MAVS cDNAs from several fish species (including salmon and zebrafish) and showed that they were orthologs of mammalian MAVS. We demonstrated that overexpression of these mitochondrial proteins in fish cells led to a constitutive induction of IFN and IFN-stimulated genes (ISGs). MAVS-overexpressing cells were almost fully protected against RNA virus infection, with a strong inhibition of both DNA and RNA virus replication (1,000- and 10,000-fold decreases, respectively). Analyses of MAVS deletion mutants showed that both the N-terminal CARD-like and C-terminal transmembrane domains, but not the central proline-rich region, were indispensable for MAVS signaling function. In addition, we cloned the cDNAs encoding a RIG-I-like molecule from salmonid and cyprinid cell lines. Like the case with MAVS, overexpression of RIG-I CARDs in fish cells led to a strong induction of both IFN and ISGs, conferring on fish cells full protection against RNA virus infection. This report provides the first demonstration that teleost fish possess a functional RLR pathway in which MAVS may play a central role in the induction of the innate immune response.
journal_name
J Viroljournal_title
Journal of virologyauthors
Biacchesi S,LeBerre M,Lamoureux A,Louise Y,Lauret E,Boudinot P,Brémont Mdoi
10.1128/JVI.00404-09subject
Has Abstractpub_date
2009-08-01 00:00:00pages
7815-27issue
16eissn
0022-538Xissn
1098-5514pii
JVI.00404-09journal_volume
83pub_type
杂志文章abstract::The covalent linkage of oncornavirus-specific DNA to chicken DNA was investigated in normal chicken embryo fibroblasts (CEF) and in virus-producing leukemic cells transformed by avian myeloblastosis virus (AMV). The virus-specific sequences present in cellular DNA fractionated by different methods were detected by DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.721-732.1973
更新日期:1973-10-01 00:00:00
abstract::Recent in vitro proteomics screens revealed that many host proteins could interact with the replication proteins of Tomato bushy stunt virus (TBSV), which is a small, plus-stranded RNA virus (Z. Li, D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy, J. Virol. 82:6911-6926, 2008). To further our understanding of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00789-09
更新日期:2009-11-01 00:00:00
abstract::Maintenance of Kaposi's sarcoma-associated herpesvirus (KSHV) latent infection depends on the viral episomes in the nucleus being distributed to daughter cells following cell division. The latency-associated nuclear antigen (LANA) is constitutively expressed in all KSHV-infected cells. LANA binds sequences in the term...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11596-11604.2002
更新日期:2002-11-01 00:00:00
abstract::Chemokine receptors CCR5 and CXCR4 are the primary fusion coreceptors utilized for CD4-mediated entry by macrophage (M)- and T-cell line (T)-tropic human immunodeficiency virus type 1 (HIV-1) strains, respectively. Here we demonstrate that HIV-1 Tat protein, a potent viral transactivator shown to be released as a solu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8952-8960.1998
更新日期:1998-11-01 00:00:00
abstract::Leishmania RNA virus (LRV) is a double-stranded RNA virus that infects some strains of the protozoan parasite leishmania As with other totiviruses, LRV presumably expresses its polymerase by a ribosomal frameshift, resulting in a capsid-polymerase fusion protein. We have demonstrated previously that an LRV capsid-poly...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10448-10455.2003
更新日期:2003-10-01 00:00:00
abstract::The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01512-09
更新日期:2010-01-01 00:00:00
abstract::Semlike forest virus capsid protein cosedimented with the large ribosomal subunit at 60S in sucrose gradients after treatment of cytoplasm from infected cells with Triton X-100 and EDTA. In CsCl gradients the capsid protein banded with the subunit at a density of 1.56 to 1.57 g/cm3. Most of the capsid protein could be...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.203-210.1976
更新日期:1976-10-01 00:00:00
abstract::Foamy viruses (FVs) generate their Pol protein precursor molecule independently of the Gag protein from a spliced mRNA. This mode of expression raises the question of the mechanism of Pol protein incorporation into the viral particle (capsid). We previously showed that the packaging of (pre)genomic RNA is essential fo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7005-7013.2005
更新日期:2005-06-01 00:00:00
abstract::The immune response to viral glycoproteins is often directed against conformation- and/or glycosylation-dependent structures; synthetic peptides and bacterially expressed proteins are inadequate probes for the mapping of such epitopes. This report describes a retroviral vector system that presents such native epitopes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.400-410.1994
更新日期:1994-01-01 00:00:00
abstract::Sequences which encode Epstein-Barr virus (EBV) RNA in nasopharyngeal carcinoma (NPC) tissue have been identified. We utilized human biopsy material directly as well as NPC grown in nude mice. Total RNA was extracted from the tumor material and separated into polyadenylated and nonpolyadenylated fractions by oligodeox...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.48.3.580-590.1983
更新日期:1983-12-01 00:00:00
abstract::Positional homologs to the UL51 open reading frame of herpes simplex virus type 1 have been identified throughout the herpesvirus family. However, no respective protein has so far been described for any of the herpesviruses. With rabbit antisera directed against oligopeptides predicted to comprise antigenic regions of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5635-5638.1997
更新日期:1997-07-01 00:00:00
abstract::An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2305-2309.1998
更新日期:1998-03-01 00:00:00
abstract:UNLABELLED:The lipid kinase phosphatidylinositol 4-kinase III alpha (PI4KIIIα) is an endoplasmic reticulum (ER)-resident enzyme that synthesizes phosphatidylinositol 4-phosphate (PI4P). PI4KIIIα is an essential host factor for hepatitis C virus (HCV) replication. Interaction with HCV nonstructural protein 5A (NS5A) lea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01063-14
更新日期:2014-09-01 00:00:00
abstract::We have found that the replicative helicase E1 of bovine papillomavirus type 1 (BPV-1) interacts with a key cell cycle regulator of S phase, the cyclin E-Cdk2 kinase. The E1 helicase, which interacts with cyclin E and not with Cdk2, presents the highest affinity for catalytically active kinase complexes. In addition, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7255-7262.1998
更新日期:1998-09-01 00:00:00
abstract::Dengue virus (DENV) and hepatitis C virus (HCV), members of the family Flaviviridae, are global human health concerns. As positive-strand RNA viruses, they each replicate in the cytoplasm of infected cells and induce distinct membranous replication compartments where most, if not all, steps of the viral life cycle occ...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.03404-13
更新日期:2014-06-01 00:00:00
abstract::Murine coronavirus mouse hepatitis virus (MHV) causes persistent infection of the central nervous system (CNS) in rodents, which has been associated with demyelination. However, the precise mechanism of MHV persistence in the CNS remains elusive. Here we show that the progenitor oligodendrocytes (central glial 4 [CG-4...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.47-56.2005
更新日期:2005-01-01 00:00:00
abstract::The effectiveness of attenuated poliovirus vaccines when given orally to induce both systemic and mucosal immune responses against poliovirus has resulted in an effort to develop poliovirus-based vectors to express foreign proteins. We have previously described the construction of poliovirus genomes (referred to as re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2643-2649.1996
更新日期:1996-04-01 00:00:00
abstract::Five human recombinant Fab fragments (Fabs) specific for measles virus (MV) proteins were isolated from three antibody phage display libraries generated from RNAs derived from bone marrow or splenic lymphocytes from three MV-immune individuals. All Fabs reacted in an enzyme-linked immunosorbent assay with MV antigens....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.251-258.2002
更新日期:2002-01-01 00:00:00
abstract::Prasinoviruses infecting unicellular green algae in the order Mamiellales (class Mamiellophyceae) are commonly found in coastal marine waters where their host species frequently abound. We tested 40 Ostreococcus tauri viruses on 13 independently isolated wild-type O. tauri strains, 4 wild-type O. lucimarinus strains, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07221-11
更新日期:2012-04-01 00:00:00
abstract::The region of the UL component of the herpes simplex virus type 1 genome between nucleotides 17,793 and 25,150 includes three open reading frames that code for the protein products of the UL8, UL9, and UL10 genes (D.J. McGeogh, M.A. Dalrymple, A.J. Davison, A. Dolan, M.C. Frame, D. McNab, L.J. Perry, J.E. Scott, and P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4251-4261.1994
更新日期:1994-07-01 00:00:00
abstract::We set out to test the hypothesis that interleukin-22 (IL-22), a cytokine crucial for epithelial cell homeostasis and recovery from tissue injury, would be protective during influenza virus infection. Recent studies have identified phenotypically and functionally unique intestinal NK cells capable of producing the cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00187-10
更新日期:2010-08-01 00:00:00
abstract::The identification of tumor-inducing genes is a driving force for elucidating the molecular mechanisms underlying cancer. Many retroviruses induce tumors by insertion of viral DNA adjacent to cellular oncogenes, resulting in altered expression and/or structure of the encoded proteins. The availability of the mouse gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.2056-2062.2003
更新日期:2003-02-01 00:00:00
abstract::pp60v-src is a nonreceptor protein tyrosine kinase that can transform both chicken and rodent fibroblasts. The src homology 2 (SH2) domain of this protein serves a critical role in the regulation of protein tyrosine kinase activity. The host range proteins pp60v-src-L, which contains a deletion of a highly conserved r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7267-7274.1994
更新日期:1994-11-01 00:00:00
abstract::The entire genome of the reference strain of bluetongue virus (BTV) serotype 16 (strain RSArrrr/16) was sequenced (a total of 23,518 base pairs). The virus was obtained from the Orbivirus Reference Collection (ORC) at IAH, Pirbright, United Kingdom. The virus strain, which was previously provided by the Onderstepoort ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01672-12
更新日期:2012-09-01 00:00:00
abstract::Borna disease virus infection is diagnosed by the presence of serum antibodies reactive with the major viral proteins, p40 and p23. Although p40 and p23 are unrelated in amino acid sequence structure, cross-reactive antibodies are described. Protein fragments and synthetic peptides were analyzed to characterize the sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.8133-8137.1996
更新日期:1996-11-01 00:00:00
abstract::The cytoplasmic assembly of vaccinia virus is reversibly blocked by the antibiotic rifampin, leading to the accumulation of partially membrane-delineated rifampin bodies in infected cells. Rifampin-resistant vaccinia virus mutants have point mutations in the D13L gene, which is controlled by a late promoter and expres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1103-1114.1994
更新日期:1994-02-01 00:00:00
abstract::Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. In this study, we have analyzed nucleotide sequences encompassing 629 bp at the carboxy terminus of the G glycoprotein gene for HRSV subgr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.9.4675-4683.2004
更新日期:2004-05-01 00:00:00
abstract::An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3307-3311.1997
更新日期:1997-04-01 00:00:00
abstract::Sequence analysis of the Lymantria dispar multicapsid nucleopolyhedrovirus (LdMNPV) genome identified an open reading frame (ORF) encoding a 548-amino-acid (62-kDa) protein that showed 35% amino acid sequence identity with vaccinia virus ATP-dependent DNA ligase. Ligase homologs have not been reported from other bacul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9142-9149.1998
更新日期:1998-11-01 00:00:00
abstract::Two species of basic internal proteins were found in osmotic shock supernatant solutions of bacteriophages T4B, T4D, T2H, T2L, and T6. The major species of protein isolated had a molecular weight of approximately 21,000 daltons, whereas the minor protein molecular weight was near 9,500 daltons. The two protein species...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.4.445-454.1970
更新日期:1970-10-01 00:00:00