Abstract:
:The region of the UL component of the herpes simplex virus type 1 genome between nucleotides 17,793 and 25,150 includes three open reading frames that code for the protein products of the UL8, UL9, and UL10 genes (D.J. McGeogh, M.A. Dalrymple, A.J. Davison, A. Dolan, M.C. Frame, D. McNab, L.J. Perry, J.E. Scott, and P. Taylor, J. Gen. Virol. 69:1531-1574, 1988). We have mapped and characterized the overlapping transcripts in this region and have found that, in addition to the low-abundance UL8 and UL9 transcripts and the abundant UL10 transcript, at least two additional transcription units, designated UL8.5 and UL9.5, are specified by this region of the genome. The 5' ends of the UL8, UL8.5, and UL9 transcripts were mapped to nucleotides 20,682, 22,351, and 23,381, respectively. The 5' terminus of the UL9.5 transcript has not yet been mapped. The 3' ends of the UL8, UL8.5, UL9, and UL9.5 transcripts are coterminal at nucleotide 18,197. The 5' end of the UL10 mRNA, which is transcribed from the strand opposite that specifying the UL8, UL8.5, UL9, and UL9.5 transcripts, lies within the UL9 open reading frame at nucleotide 22,944, while the 3' terminus was mapped to nucleotide 24,666. Time course studies demonstrated that the UL8 and UL9 transcripts are members of the early kinetic class, the UL8.5 mRNA is a delayed-early transcript, and the UL9.5 and UL10 transcripts belong to the true-late kinetic class. Examination of the nucleotide sequence of the UL8.5 transcript revealed a potential open reading frame that overlaps and is in frame with the C-terminal half of the open reading frame encoding the origin-binding protein (OBP), the product of the UL9 gene. In vitro translation of the UL8.5 transcript demonstrated that it encodes a protein with an apparent molecular mass of 53 kDa. This protein was recognized by antibody directed against the C-terminal region of OBP and has thus been designated OBPC. A protein with an identical apparent molecular mass was also recognized by this antibody in infected-cell lysates, indicating that OBPC is synthesized during viral infection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Baradaran K,Dabrowski CE,Schaffer PAdoi
10.1128/JVI.68.7.4251-4261.1994subject
Has Abstractpub_date
1994-07-01 00:00:00pages
4251-61issue
7eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for KS tumors, multicentric Castleman's disease, and primary effusion lymphomas. Like other herpesvirus capsids, the KSHV capsid is an icosahedral structure composed of six proteins. The capsid shell is made up of the major capsid protein, two tripl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00423-08
更新日期:2008-07-01 00:00:00
abstract::A variant of human T-cell leukemia virus subgroup I (HTLV-I), designated HTLV-Ib, has been isolated from a transformed T-lymphocytic cell line established from a Zairian patient with adult T-cell lymphoma. A recombinant phage clone of the variant provirus, denoted lambda MC-1, hybridizes under high stringency to HTLV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.781-790.1985
更新日期:1985-06-01 00:00:00
abstract::A fresh inoculum of human adenovirus type 12 (Ad12) was obtained from the American Type Culture Collection and passaged once on human embryonic kidney cells, and Ad12 DNA was prepared from the first-passage yield to avoid higher passages which might have generated host-virus DNA recombinants. The 18 PstI fragments of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.379-389.1994
更新日期:1994-01-01 00:00:00
abstract::Lentivectors stimulate potent immune responses to antigen transgenes and are being developed as novel genetic vaccines. To improve safety while retaining efficacy, we constructed a lentivector in which transgene expression was restricted to antigen-presenting cells using the mouse dectin-2 gene promoter. This lentivec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01289-07
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abstract::Borna disease virus (BDV) is a nonsegmented, negative-strand RNA virus that causes neurological diseases in a variety of warm-blooded animal species. There is general consensus that BDV can also infect humans, being a possible zoonosis. Although the clinical consequences of human BDV infection are still controversial,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6268-6286.2002
更新日期:2002-06-01 00:00:00
abstract::A hallmark of infection with herpes simplex virus type 1 (HSV-1) is the establishment of latency in ganglia of the infected individual. During the life of the latently infected individual, the virus can occasionally reactivate, travel back to the eye, and cause recurrent disease. Indeed, a major cause of corneal scarr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02234-08
更新日期:2009-03-01 00:00:00
abstract::Parapoxviruses can be morphologically distinguished from other poxviruses in conventional negative staining electron microscopy (EM) by their ovoid appearance and the spiral tubule surrounding the virion's surface. However, this technique may introduce artifacts. We have examined Orf virus (ORFV; the prototype species...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.8085-8093.2004
更新日期:2004-08-01 00:00:00
abstract::Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on AAVS1 is thought to be an essential step, the mechanism of the site-specific integration, particularly, how the site of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02014-06
更新日期:2007-02-01 00:00:00
abstract::Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7006-7014.1995
更新日期:1995-11-01 00:00:00
abstract::Viruses with spindle-shaped virions are abundant in diverse environments. Over the years, such viruses have been isolated from a wide range of archaeal hosts. Evolutionary relationships between them remained enigmatic, however. Here, using structural proteins as markers, we define familial ties among these "dark horse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02941-13
更新日期:2014-02-01 00:00:00
abstract::Murine gammaherpesvirus 68 (gammaHV68, or MHV-68) is a genetically tractable, small animal model for the analysis of gammaherpesvirus pathogenesis. The gammaHV68 genome is colinear with the genomes of other sequence gammaherpesviruses, containing large blocks of conserved genes interspersed by a number of putative gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.4.1973-1984.2000
更新日期:2000-02-01 00:00:00
abstract::Epitope-specific CD8+ T lymphocytes may play an important role in controlling human immunodeficiency virus (HIV)/simian immunodeficiency virus replication. Unfortunately, standard cellular assays do not measure the antiviral efficacy (the ability to suppress virus replication) of CD8+ T lymphocytes. Certain epitope-sp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.23.14986-14991.2005
更新日期:2005-12-01 00:00:00
abstract::Hemagglutinin (HA) of H3N2/1968 pandemic influenza viruses differs from the putative avian precursor by seven amino acid substitutions. Substitutions Q226L and G228S are known to be essential for adaptation of avian HA to mammals. We found that introduction of avian-virus-like amino acids at five other HA positions (p...
journal_title:Journal of virology
pub_type: 历史文章,杂志文章
doi:10.1128/JVI.01292-15
更新日期:2015-12-01 00:00:00
abstract::The interaction of the human immunodeficiency virus type 1 (HIV-1) Pr55Gag molecule with the plasma membrane of an infected cell is an essential step of the viral life cycle. Myristic acid and positively charged residues within the N-terminal portion of MA constitute the membrane-binding domain of Pr55Gag. A separate ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.2723-2732.1998
更新日期:1998-04-01 00:00:00
abstract::Monocytes/macrophages (MO/Mphi) are the major target cells for both dengue virus (DV) and bacterial lipopolysaccharide (LPS), and the aim of this study was to define their interactions. We had found that LPS markedly suppressed DV infection of primary human MO/Mphi when it was added to cultures prior to or together wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2650-2657.1999
更新日期:1999-04-01 00:00:00
abstract::The mammalian tripartite motif protein, TRIM5α, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5α protein and retrovirus, complete disruption of the TRIM5α RING domain decreases virus-restricting activity to various degrees. TRIM5α exhibits RING domain-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00341-11
更新日期:2011-08-01 00:00:00
abstract::In contrast to humans, several primate species are believed to have harbored simian immunodeficiency viruses (SIVs) since ancient times. In particular, the geographically dispersed species of African green monkeys (AGMs) are all infected with highly diversified SIVagm viruses at high prevalences (greater than 50% of s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8449-8460.2001
更新日期:2001-09-01 00:00:00
abstract::While RNA silencing is a potent antiviral defense in plants, well-adapted plant viruses are known to encode suppressors of RNA silencing (VSR) that can neutralize the effectiveness of RNA silencing. As a result, most plant genes involved in antiviral silencing were identified by using debilitated viruses lacking silen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00497-12
更新日期:2012-06-01 00:00:00
abstract::The Moloney murine leukemia virus (MuLV) is a highly leukemogenic virus. To map the leukemogenic potential of Moloney MuLV, we constructed chimeric viral DNA genomes in vitro between parental cloned infectious viral DNA from Moloney and amphotropic 4070-A MuLVs. Infectious chimeric MuLVs were recovered by microinjecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.448-456.1984
更新日期:1984-11-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) remains latent throughout the life of the carrier, with cells containing the provirus and viral gene expression efficiently down-regulated. On a molecular level, exactly how viruses are down-regulated in vivo remains unresolved. We described here the possibility that down-re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4485-4494.1997
更新日期:1997-06-01 00:00:00
abstract::Members of the Paramyxovirinae subfamily rely on the concerted action of two envelope glycoprotein complexes, attachment protein H and the fusion (F) protein oligomer, to achieve membrane fusion for viral entry. Despite advances in X-ray information, the organization of the physiological attachment (H) oligomer in fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01222-10
更新日期:2010-12-01 00:00:00
abstract::The isolation and characterization of an adenovirus mutant, Ad5dl802r1, containing two independent deletions in the 72-kilodalton (kDa) DNA-binding protein (DBP) gene is described. The two deletions remove amino acids 23 through 105 of DBP, resulting in the production of a 50-kDa product. Expression of this truncated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.2289-2299.1989
更新日期:1989-05-01 00:00:00
abstract::HIV-1 causes a persistent infection of the immune system that is associated with chronic comorbidities. The mechanisms that underlie this inflammation are poorly understood. Emerging literature has implicated proinflammatory purinergic receptors and downstream signaling mediators in HIV-1 infection. This study probed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01186-18
更新日期:2018-12-10 00:00:00
abstract:UNLABELLED:The increasing prevalence of ranavirus (RV; Iridoviridae) infections of wild and commercially maintained aquatic species is raising considerable concerns. While Xenopus laevis is the leading model for studies of immunity to RV, amphibian antiviral interferon (IFN) responses remain largely uncharacterized. Ac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00223-14
更新日期:2014-05-01 00:00:00
abstract::Western equine encephalitis virus (WEEV; Togaviridae, Alphavirus) is an enveloped RNA virus that is typically transmitted to vertebrate hosts by infected mosquitoes. WEEV is an important cause of viral encephalitis in humans and horses in the Americas, and infection results in a range of disease, from mild flu-like il...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00876-10
更新日期:2010-10-01 00:00:00
abstract::AP-1- and ATF-binding sites are cis-acting transcriptional elements within the U3 domain of the feline immunodeficiency virus (FIV) long terminal repeat (LTR) that serve as targets for cellular activation pathways and may regulate virus replication. We report that FIV LTR mutant proviruses encoding U3 deletions of the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.2.1054-1060.2001
更新日期:2001-01-01 00:00:00
abstract::We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1924-1928.1989
更新日期:1989-05-01 00:00:00
abstract::Measles virus (MV) infection causes an acute childhood disease, associated in certain cases with infection of the central nervous system and development of a severe neurological disease. We have generated transgenic mice ubiquitously expressing the human protein SLAM (signaling lymphocytic activation molecule), or CD1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00209-06
更新日期:2006-07-01 00:00:00
abstract::The establishment of de novo-generated patterns of DNA methylation is characterized by the gradual spreading of DNA methylation (I. Kuhlmann and W. Doerfler, J. Virol. 47:631-636, 1983; M. Toth, U. Lichtenberg, and W. Doerfler, Proc. Natl. Acad. Sci. USA 86:3728-3732, 1989; M. Toth, U. Müller, and W. Doerfler J. Mol. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4301-4308.1991
更新日期:1991-08-01 00:00:00
abstract::An important site for bovine herpesvirus 1 (BoHV-1) latency is sensory neurons within trigeminal ganglia (TG). The synthetic corticosteroid dexamethasone consistently induces BoHV-1 reactivation from latency. Expression of four Krüppel-like transcription factors (KLF), i.e., KLF4, KLF6, PLZF (promyelocytic leukemia zi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01670-19
更新日期:2020-01-31 00:00:00