Appearance of the bona fide spiral tubule of ORF virus is dependent on an intact 10-kilodalton viral protein.


:Parapoxviruses can be morphologically distinguished from other poxviruses in conventional negative staining electron microscopy (EM) by their ovoid appearance and the spiral tubule surrounding the virion's surface. However, this technique may introduce artifacts. We have examined Orf virus (ORFV; the prototype species of the Parapoxvirus genus) by cryoelectron microscopy (cryo-EM) and cryo-negative staining EM. From these studies we suggest that the shape and unique spiral tubule are authentic features of the parapoxviruses. We also constructed an ORFV mutant deleted of a gene encoding a 10-kDa protein, which is an orthologue of the vaccinia virus (VACV) 14-kDa fusion protein, and investigated its ultrastructure. This mutant virus multiplied slowly in permissive cells and produced infectious but morphologically aberrant particles. Mutant virions lacked the spiral tubule but displayed short disorganized tubules similar to those observed on the surface of VACV. In addition, thin extensions or loop-like structures were appended to the ORFV mutant particles. We suggest that these appended structures arise from a failure of the mutant virus particles to properly seal and that the sealing activity is dependent on the 10-kDa protein.


J Virol


Journal of virology


Spehner D,De Carlo S,Drillien R,Weiland F,Mildner K,Hanau D,Rziha HJ




Has Abstract


2004-08-01 00:00:00














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    abstract::The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) produces multiple mRNAs through differential splicing and polyadenylation. Reverse transcriptase PCR was used to characterize transcripts from exons 1, 2, 3, and 4 (immediate-early 1 [IE1]). The expected IE72 and IE19 mRNAs were detected, as well as ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Awasthi S,Isler JA,Alwine JC

    更新日期:2004-08-01 00:00:00

  • Activation of ATPase activity of simian virus 40 large T antigen by the covalent affinity analog of ATP, fluorosulfonylbenzoyl 5'-adenosine.

    abstract::Fluorosulfonylbenzoyl 5'-adenosine (FSBA) bound to one site in simian virus 40 large T antigen (T) and covalently modified greater than 95% of the molecules in a complete reaction. This analog for ATP specifically cross-links to the Mg-phosphate pocket in ATP-binding sites. Cyanogen bromide cleavage and tryptic digest...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bradley MK

    更新日期:1990-10-01 00:00:00

  • Isolation and characterization of two basic internal proteins from the T-even bacteriophages.

    abstract::Two species of basic internal proteins were found in osmotic shock supernatant solutions of bacteriophages T4B, T4D, T2H, T2L, and T6. The major species of protein isolated had a molecular weight of approximately 21,000 daltons, whereas the minor protein molecular weight was near 9,500 daltons. The two protein species...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Stone KR,Cummings DJ

    更新日期:1970-10-01 00:00:00

  • Nuclear factor I and epithelial cell-specific transcription of human papillomavirus type 16.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Apt D,Chong T,Liu Y,Bernard HU

    更新日期:1993-08-01 00:00:00

  • Truncated particles produced in fish surviving infectious hematopoietic necrosis virus infection: mediators of persistence?

    abstract::Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that produces an acute, lethal infection in rainbow trout (Oncorhynchus mykiss). Fish that survive infection cease to produce detectable infectious virus at approximately 46 days after infection, yet there is evidence that survivor fish continue to harbor...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kim CH,Dummer DM,Chiou PP,Leong JA

    更新日期:1999-01-01 00:00:00

  • Genome organization and reverse genetic analysis of a type I feline coronavirus.

    abstract::In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tekes G,Hofmann-Lehmann R,Stallkamp I,Thiel V,Thiel HJ

    更新日期:2008-02-01 00:00:00

  • Palmitoylated Cysteines in Chikungunya Virus nsP1 Are Critical for Targeting to Cholesterol-Rich Plasma Membrane Microdomains with Functional Consequences for Viral Genome Replication.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bakhache W,Neyret A,Bernard E,Merits A,Briant L

    更新日期:2020-05-04 00:00:00

  • Identification of novel positive-strand RNA viruses by metagenomic analysis of archaea-dominated Yellowstone hot springs.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bolduc B,Shaughnessy DP,Wolf YI,Koonin EV,Roberto FF,Young M

    更新日期:2012-05-01 00:00:00

  • Hepatitis B virus subviral envelope particle morphogenesis and intracellular trafficking.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Patient R,Hourioux C,Sizaret PY,Trassard S,Sureau C,Roingeard P

    更新日期:2007-04-01 00:00:00

  • Transformation-defective mutants of Rous sarcoma virus with longer sizes of genome RNA and their highly frequent occurrences.

    abstract::Transformation-defective (td) mutants with different sizes of genomic RNA were isolated from the Prague strain of Rous sarcoma virus, subgroup C(PR-C). All six td viruses (tdTYPR-C) isolated from a single UV-irradiated stock of PR-C (clone 2 of TYPR-C) had slightly longer RNA than did the ordinary class b RNA of tdB77...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yoshida M,Yamashita M,Nomoto A

    更新日期:1979-05-01 00:00:00

  • Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein.

    abstract:UNLABELLED:Microtubule transport of circovirus from the periphery of the cell to the nucleus is essential for viral replication in early infection. How the microtubule is recruited to the viral cargo remains unclear. In this study, we observed that circovirus trafficking is dependent on microtubule polymerization and t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Cao J,Lin C,Wang H,Wang L,Zhou N,Jin Y,Liao M,Zhou J

    更新日期:2015-03-01 00:00:00

  • Sterol regulatory element-binding protein 2 couples HIV-1 transcription to cholesterol homeostasis and T cell activation.

    abstract::Cholesterol plays an essential role in the life cycle of several enveloped viruses. Many of these viruses manipulate host cholesterol metabolism to facilitate their replication. HIV-1 infection of CD4(+) T cells activates the sterol regulatory element-binding protein 2 (SREBP2) transcriptional program, which includes ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Taylor HE,Linde ME,Khatua AK,Popik W,Hildreth JE

    更新日期:2011-08-01 00:00:00

  • Interactions between E6, FAK, and GIT1 at paxillin LD4 are necessary for transformation by bovine papillomavirus 1 E6.

    abstract:UNLABELLED:Bovine papillomavirus 1 E6 interacts with two similar proteins that regulate cell attachment and cell migration called paxillin (PXN) and HIC-5 (also known as HIC5, ARA55, HIC-5, TSC-5, and TGFB1I1). Despite the similarity between HIC-5 and paxillin, paxillin is required for E6 to transform mouse embryo fibr...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Brimer N,Wade R,Vande Pol S

    更新日期:2014-09-01 00:00:00

  • Two novel promoters in the upstream regulatory region of human papillomavirus type 31b are negatively regulated by epithelial differentiation.

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ozbun MA,Meyers C

    更新日期:1999-04-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Robalino J,Browdy CL,Prior S,Metz A,Parnell P,Gross P,Warr G

    更新日期:2004-10-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Udem SA,Cook KA

    更新日期:1984-01-01 00:00:00

  • Altered Rous sarcoma virus Gag polyprotein processing and its effects on particle formation.

    abstract::Proteolytic processing of the Rous sarcoma virus (RSV) Gag precursor was altered in vivo through the introduction of amino acid substitutions into either the polyprotein cleavage junctions or the PR coding sequence. Single amino acid substitutions (V(P2)S and P(P4)G), which are predicted from in vitro peptide substrat...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Xiang Y,Ridky TW,Krishna NK,Leis J

    更新日期:1997-03-01 00:00:00

  • Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract.

    abstract::In an earlier study on minus-strand DNA synthesis catalyzed by murine leukemia virus reverse transcriptase, we described a prominent pause site near the polypurine tract (J. Guo, W. Wu, Z. Y. Yuan, K. Post, R. J. Crouch, and J. G . Levin, Biochemistry 34:5018-5029, 1995). We now report that pausing at this site is due...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wu W,Henderson LE,Copeland TD,Gorelick RJ,Bosche WJ,Rein A,Levin JG

    更新日期:1996-10-01 00:00:00

  • Base composition and hybridization studies of the three double-stranded RNA segments of bacteriophage phi 6.

    abstract::The three double-stranded ribonucleic acid (dsRNA) segments of the bacteriophage phi6 were isolated and shown to have similar melting temperatures and base compositions. RNA:RNA hybridization experiments with the isolated segments eliminate the possibility that the two smaller dsRNA segments arise from a cleavage of t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Van Etten JL,Vidaver AK,Koski RK,Burnett JP

    更新日期:1974-06-01 00:00:00

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    abstract::The ribonucleoprotein (RNP) internal components of influenza virus were separated into distinct size classes by sedimentation in glycerol gradients and examined by electron microscopy by using positive staining with uranyl acetate. The large RNP have a peak in length distribution at 90 to 110 nm, the medium, at 60 to ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Compans RW,Content J,Duesberg PH

    更新日期:1972-10-01 00:00:00

  • Human Cytomegalovirus UL111A and US27 Gene Products Enhance the CXCL12/CXCR4 Signaling Axis via Distinct Mechanisms.

    abstract::Human cytomegalovirus (HCMV) is a prevalent pathogen that establishes lifelong infection in the host. Virus persistence is aided by extensive manipulation of the host immune system, particularly cytokine and chemokine signaling pathways. The HCMV UL111A gene encodes cmvIL-10, an ortholog of human interleukin-10 that h...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tu CC,Arnolds KL,O'Connor CM,Spencer JV

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    pub_type: 杂志文章


    authors: Karsten CB,Buettner FF,Cajic S,Nehlmeier I,Neumann B,Klippert A,Sauermann U,Reichl U,Gerardy-Schahn R,Rapp E,Stahl-Hennig C,Pöhlmann S

    更新日期:2015-11-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Fodor E,Pritlove DC,Brownlee GG

    更新日期:1994-06-01 00:00:00

  • Estradiol regulates susceptibility following primary exposure to genital herpes simplex virus type 2, while progesterone induces inflammation.

    abstract::We report here that sex hormones modulate susceptibility to a sexually transmitted viral agent, herpes simplex virus type 2 (HSV-2), in a mouse model. Ovariectomized mice were administered either saline (control), estradiol (E(2)), progesterone (P(4)), or a combination of both estradiol and progesterone (E+P) and infe...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gillgrass AE,Fernandez SA,Rosenthal KL,Kaushic C

    更新日期:2005-03-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Mselle TF,Howell AL,Ghosh M,Wira CR,Sentman CL

    更新日期:2009-11-01 00:00:00

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    abstract::A pediatric human immunodeficiency virus type 1 (HIV-1) vaccine would be desirable to protect infants against HIV-1 transmission from breast-feeding. Such a vaccine would need to induce protective immunity at mucosal surfaces in neonates as soon as possible after birth. Recombinant adenovirus (rAd) vectors have been s...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Liu J,Li H,Iampietro MJ,Barouch DH

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Liu C,Tang J,Ma Y,Liang X,Yang Y,Peng G,Qi Q,Jiang S,Li J,Du L,Li F

    更新日期:2015-06-01 00:00:00

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    abstract::Two live attenuated single-deletion mutant simian immunodeficiency virus (SIV) constructs, SIV239Deltanef and SIVPBj6.6Deltanef, were tested for their abilities to stimulate protective immunity in macaques. During the immunization period the animals were examined for specific immune responses and virus growth. Each co...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Lewis MG,Yalley-Ogunro J,Greenhouse JJ,Brennan TP,Jiang JB,VanCott TC,Lu Y,Eddy GA,Birx DL

    更新日期:1999-02-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Morello CS,Levinson MS,Kraynyak KA,Spector DH

    更新日期:2011-04-01 00:00:00

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    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Seiler P,Senn BM,Klenerman P,Kalinke U,Hengartner H,Zinkernagel RM

    更新日期:2000-07-01 00:00:00