Abstract:
:The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of the cl-2 S protein cDNA. Recombinant vaccinia viruses containing the cl-2 S cDNA [RVV t(+)] or the mutated cDNA [RVV t(-)] were constructed and monitored for fusion formation and cleavage of the expressed S proteins. When cultured DBT cells were infected with RVV t(+) at a multiplicity of infection of 0.5, fusion formation was first observed at 10 to 12 h postinoculation and spread throughout the whole culture by 20 to 24 h postinoculation. In cells infected with RVV t(-) under the same conditions, fusion formation appeared by 12 to 14 h. This result represented a 2- to 4-h delay in the onset of fusion, compared with its appearance in cells expressing the wild-type S protein. By 25 to 30 h, most of the cells infected by RVV t(-) had fused. By immunoprecipitation and Western blotting (immunoblotting), the 170-kDa S protein was detected in DBT cells expressing the wild-type S protein and the mutated S protein. However, interestingly, the cleavage products of the S protein, S1 and S2, were not detected in RVV t(-)-infected cells, producing the mutated S protein, even though fusion was clearly visible. Both products were, of course, detected in RVV t(+)-infected DBT cells, producing the wild-type S protein. The same results concerning the fusion formation and cleavage properties of the S proteins were reproduced by the transiently expressed S proteins. These results suggest that the cleavage event in the S protein of murine coronavirus JHMV is not a prerequisite for fusion formation but that it does facilitate fusion formation.
journal_name
J Viroljournal_title
Journal of virologyauthors
Taguchi Fdoi
10.1128/JVI.67.3.1195-1202.1993subject
Has Abstractpub_date
1993-03-01 00:00:00pages
1195-202issue
3eissn
0022-538Xissn
1098-5514journal_volume
67pub_type
杂志文章abstract:UNLABELLED:Influenza A viruses (IAVs) rely on host factors to support their life cycle, as viral proteins hijack or interact with cellular proteins to execute their functions. Identification and understanding of these factors would increase our knowledge of the molecular mechanisms manipulated by the viruses. In this s...
journal_title:Journal of virology
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abstract::Transgene expression after the administration of recombinant adenovirus with E1 deleted is constantly transient. It is admitted that E1A-substituting activities of cellular or viral origin allow viral antigen synthesis and trigger cytotoxic lymphocyte-mediated clearance of the recipient cells. Our approach to solving ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.1.559-565.1996
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pub_type: 杂志文章
doi:10.1128/JVI.00381-07
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1194-1202.1988
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abstract::Human metapneumovirus (hMPV) is a respiratory paramyxovirus of global clinical relevance. Despite the substantial knowledge generated during the last 10 years about hMPV infection, information regarding the activation of the immune response against this virus remains largely unknown. In this study, we demonstrated tha...
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.11.5640-5644.1990
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.18.9069-9078.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3005-3012.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.253-264.1973
更新日期:1973-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.12.5629-5638.2000
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00659-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.290-292.1984
更新日期:1984-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02639-07
更新日期:2008-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02762-13
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00049-07
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abstract::The parvovirus minute virus of mice (MVM) packages predominantly negative-sense single strands, while its close relative LuIII encapsidates strands of both polarities with equal efficiency. Using genomic chimeras and mutagenesis, we show that the ability to package positive strands maps not, as originally postulated, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.4.2287-2300.2005
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1941-1952.1996
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7341-7348.1998
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pub_type: 杂志文章
doi:10.1128/JVI.72.4.3401-3406.1998
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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abstract::Adenovirus infection affects the nuclear distribution of host splicing factors. Late phase-infected cells contain discrete clusters of small nuclear ribonucleoproteins (snRNPs) that are separate from centers containing the viral 72-kilodalton DNA-binding protein (72K protein). In the present study, we demonstrate that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.281-290.1995
更新日期:1995-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6582-6592.1997
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abstract::Vesicular stomatitis virus (VSV) is a prototype nonsegmented, negative-sense virus used to examine viral functions of a broad family of viruses, including human pathogens. Here we demonstrate that S(2) VSV, an isolate with a small plaque phenotype compared to other Indiana strain viruses, has a transcription defect re...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8588-8592.2003
更新日期:2003-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.186-193.1985
更新日期:1985-04-01 00:00:00
abstract::ISG15, a 15-kDa interferon-induced protein that participates in antiviral defenses of mammals, is highly conserved among vertebrates. In fish, as in mammals, viral infection and interferon treatment induce isg15 expression. The two ubiquitin-like domains of ISG15 and the presence of a consensus LRLRGG sequence in the ...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00