Abstract:
:Previous studies have suggested that coxsackievirus B (CVB) activates CD8(+) T cells in vivo, but the extent of this activation and the antigen specificity of the CD8(+) T cells remain uncertain. Furthermore, CVB-induced CD4(+) T-cell responses have not been carefully investigated. Herein, we evaluate CD8(+) and CD4(+) T-cell responses both in a secondary lymphoid organ (spleen) and in peripheral tissues (heart and pancreas), using a recombinant CVB3 (rCVB3.6) that encodes well-characterized CD8(+) and CD4(+) T-cell epitopes. Despite reaching high levels in vivo, rCVB3.6 failed to trigger a marked expansion of CD8(+) or CD4(+) T cells, and T-cell activation was surprisingly limited. Furthermore, epitope-specific effector functions could not be detected using highly sensitive in vivo and ex vivo assays. Moreover, major histocompatibility complex (MHC) class I tetramer analysis indicated that our inability to detect CVB3-specific CD8(+) T-cell responses could not be explained by the cells being dysfunctional. In contrast to naïve T cells, epitope-specific memory CD8(+) and CD4(+) T cells proliferated markedly, indicating that both of the rCVB3.6-encoded epitopes were presented by their respective MHC molecules in vivo. These data are consistent with the observation that several CVB3 proteins can limit the presentation of viral epitopes on the surface of infected cells and suggest that the level of MHC/peptide complex is sufficient to trigger memory but not naïve T cells. Finally, our findings have implications for the biological significance of cross-priming, a process thought by some to be important for the induction of antiviral CD8(+) T-cell responses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kemball CC,Harkins S,Whitton JLdoi
10.1128/JVI.02639-07subject
Has Abstractpub_date
2008-05-01 00:00:00pages
4331-42issue
9eissn
0022-538Xissn
1098-5514pii
JVI.02639-07journal_volume
82pub_type
杂志文章abstract::Retroviral capsid assembly can occur by either of two distinct morphogenic processes: in type C viruses, the capsid assembles and buds at the plasma membrane, while in type B and D viruses, the capsid assembles within the cytoplasm and is then transported to the plasma membrane for budding. We have previously reported...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5431-5437.1999
更新日期:1999-07-01 00:00:00
abstract::Rickard's strain of feline leukemia virus (FeLV) contains two large glycoproteins and five smaller polypeptides of molecular weights 100,000 (gp >/= 100), 70,000 (gp70), 30,000 (p30), 21,000 (p21), 15,000 (p15), 11,200 (p11), and 10,000 (p10) when chromatographed on 6% agarose in the presence of 6 M guanidine hydrochl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.2.349-365.1974
更新日期:1974-08-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.72.1.651-659.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6073-6082.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.468-474.1986
更新日期:1986-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2427-2433.1995
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.6.3451-3459.2003
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abstract::To model human immunodeficiency virus (HIV) perinatal transmission, we studied infection of simian-human immunodeficiency virus (SHIV) SF162P3 in 10 pregnant Macaca nemestrina females and their offspring. Four of nine infants born to and suckled by these dams had evidence of infection, a transmission rate of 44.4% (95...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00131-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.20.9872-9884.2001
更新日期:2001-10-01 00:00:00
abstract::PVC-211 murine leukemia virus (MuLV) is a neuropathogenic retrovirus that has undergone genetic changes from its nonneuropathogenic parent, Friend MuLV, that allow it to efficiently infect rat brain capillary endothelial cells (BCEC). To clarify the mechanism by which PVC-211 MuLV expression in BCEC induces neurologic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02343-08
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.12.7860-7867.2005
更新日期:2005-06-01 00:00:00
abstract::Human immunodeficiency virus (HIV) infects human cells by binding to surface CD4 molecules and directly fusing with the cell membrane. Although mouse cells expressing human CD4 bind HIV, they do not become infected, apparently because of a block in membrane fusion. To study this problem, we constructed a recombinant v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2149-2156.1990
更新日期:1990-05-01 00:00:00
abstract::For the identification of RNA-binding proteins that specifically interact with potato spindle tuber viroid (PSTVd), we subjected a tomato cDNA expression library prepared from viroid-infected leaves to an RNA ligand screening procedure. We repeatedly identified cDNA clones that expressed a protein of 602 amino acids. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9685-9694.2003
更新日期:2003-09-01 00:00:00
abstract::The organization of adeno-associated virus serotype 4 (AAV 4) DNA was probed by using restriction enzymes. The cleavage sites of the following enzymes were mapped: BglII, BamI, HincII, KpnI, PstI, SalI, SstI, and XhoI. The orientation of transcription on the physical cleavage map was determined by locating the fragmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.653-661.1980
更新日期:1980-09-01 00:00:00
abstract::Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced deletions into the 5' noncoding sequence (742 nucleotides long) of the genomes of the Mahoney and Sabin 1 strains of polioviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5354-5363.1989
更新日期:1989-12-01 00:00:00
abstract::Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed that PV is inactivated by the low pH of the gastric contents in mice. The addition of 3% NaHCO3 to the viral inoculum in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02675-06
更新日期:2007-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.796-804.1991
更新日期:1991-02-01 00:00:00
abstract::The cocultivation of a lung cell line from the Southeast Asian mouse Mus cervicolor with cells from heterologous species has resulted in the isolation of two new distinct type C viruses. Both viruses are endogenous to M. cervicolor and are present in multiple copies in the cellular DNA of these mice. One of the viruse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.849-862.1977
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pub_type: 杂志文章
doi:10.1128/JVI.72.11.8690-8696.1998
更新日期:1998-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00291-19
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.913-918.1984
更新日期:1984-12-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) specifically incorporates the host cell peptidyl-prolyl isomerase cyclophilin A into virions via contacts with the capsid (CA) domain of the Gag polyprotein Pr55gag. The immunosuppressant drug cyclosporin A and the nonimmunosuppressive cyclosporin A analog SDZ NIM 811 bind t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.9.5751-5757.1996
更新日期:1996-09-01 00:00:00
abstract::F proteins from baculovirus nucleopolyhedrovirus (NPV) group II members are the major budded virus (BV) viral envelope fusion proteins. They undergo furin-like proteolysis processing in order to be functional. F proteins from different baculovirus species have a long cytoplasmic tail domain (CTD), ranging from 48 (Spo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01178-06
更新日期:2006-11-01 00:00:00
abstract::During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partia...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2010-08-01 00:00:00
abstract::In this study, we investigated the expression levels of host restriction factors in six untreated HIV-1-positive patients over the course of infection. We found that the host restriction factor gene expression profile consistently increased over time and was significantly associated with CD4+ T cell activation and vir...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00166-13
更新日期:2013-05-01 00:00:00
abstract::The papillomavirus E2 protein is a critical viral regulatory protein with transcription, DNA replication, and genome maintenance functions. We have previously identified the cellular bromodomain protein Brd4 as a major E2-interacting protein and established that it participates in tethering bovine papillomavirus type ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00447-07
更新日期:2007-09-01 00:00:00
abstract::MicroRNAs (miRNAs) are key regulators of gene expression in higher eukaryotes. Recently, miRNAs have been identified from viruses with double-stranded DNA genomes. To attempt to identify miRNAs encoded by herpes simplex virus 1 (HSV-1), we applied a computational method to screen the complete genome of HSV-1 for seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00200-06
更新日期:2006-06-01 00:00:00