Abstract:
:During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partially mature particles, as the major prM-containing particles in a dengue serotype 2 virus. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage.
journal_name
J Viroljournal_title
Journal of virologyauthors
Junjhon J,Edwards TJ,Utaipat U,Bowman VD,Holdaway HA,Zhang W,Keelapang P,Puttikhunt C,Perera R,Chipman PR,Kasinrerk W,Malasit P,Kuhn RJ,Sittisombut Ndoi
10.1128/JVI.00696-10subject
Has Abstractpub_date
2010-08-01 00:00:00pages
8353-8issue
16eissn
0022-538Xissn
1098-5514pii
JVI.00696-10journal_volume
84pub_type
杂志文章abstract::Virion protein 16 (VP16) of herpes simplex virus type 1 (HSV-1) is a potent transcriptional activator of viral immediate-early (IE) genes. The VP16 activation domain can recruit various transcriptional coactivators to target gene promoters. However, the role of transcriptional coactivators in HSV-1 IE gene expression ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02349-08
更新日期:2009-04-01 00:00:00
abstract::Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approxi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7362-7366.1995
更新日期:1995-11-01 00:00:00
abstract::Influenza A H9N2 viruses are common poultry pathogens that occasionally infect swine and humans. It has been shown previously with H9N2 viruses that reassortment can generate novel viruses with increased transmissibility. Here, we demonstrate the modeling power of a novel transfection-based inoculation system to selec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02677-13
更新日期:2014-01-01 00:00:00
abstract::We previously demonstrated that human cytomegalovirus (HCMV) infection induced the activation of the cellular transcription factor NF-kappaB. Here, we investigate the mechanism for the HCMV-induced NF-kappaB activation and the role that the induced NF-kappaB plays in transactivation of the major immediate-early promot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.9.4498-4507.2004
更新日期:2004-05-01 00:00:00
abstract::The use of different viral promoters for the expression of the EBNA1 gene product appears to be a critical step in the regulation of Epstein-Barr virus latent gene expression and may reflect the extent of differentiation of B-cell hosts. Low-passage Burkitt lymphoma cell lines resemble immature B cells in that they ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6421-6431.1994
更新日期:1994-10-01 00:00:00
abstract::In order to clarify antigenic variations among various isolates of human herpesvirus 6 (HHV-6) and cross-reactivity among HHV-6, HHV-7, and human cytomegalovirus (HCMV) in the T-cell immune response, the antigenic specificity of the proliferative response mediated by 232 CD4+ human T-cell clones directed against HHV-6...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.6259-6264.1993
更新日期:1993-10-01 00:00:00
abstract::Human T-cell leukemia virus (HTLV) Tax protein has been implicated in the HTLV oncogenic process, primarily due to its pleiotropic effects on cellular genes involved in growth regulation and cell cycle control. To date, several approaches attempting to correlate Tax activation of the CREB/activating transcription fact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2655-2662.2000
更新日期:2000-03-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::The HTLV-1 singly spliced open reading frame I protein, p12(I), is highly unstable and appears to be necessary for persistent infection in rabbits. Here we demonstrate that p12(I) forms dimers through two putative leucine zipper domains and that its stability is augmented by specific proteasome inhibitors. p12(I) is u...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6460-6467.1999
更新日期:1999-08-01 00:00:00
abstract::Many of the approximately 165 proteins encoded by the African swine fever virus (ASFV) genome do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times postinfection. Deletion of this gene did ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01428-17
更新日期:2017-11-30 00:00:00
abstract::Infection of human dermal microvascular endothelial (HMVEC-d) cells and human foreskin fibroblast (HFF) cells in vitro by Kaposi's sarcoma-associated herpesvirus (KSHV) provides an excellent in vitro model system to study viral latency. KSHV infection is characterized by the induction of preexisting host signal cascad...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00231-06
更新日期:2006-07-01 00:00:00
abstract::A deletion mutant of severe acute respiratory syndrome coronavirus (SARS-CoV) has been engineered by deleting the structural E gene in an infectious cDNA clone that was constructed as a bacterial artificial chromosome (BAC). The recombinant virus lacking the E gene (rSARS-CoV-DeltaE) was rescued in Vero E6 cells. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01467-06
更新日期:2007-02-01 00:00:00
abstract::The purpose of this study was to define the relationship between herpes simplex virus (HSV) latency and in vivo ganglionic reactivation. Groups of mice with numbers of latently infected neurons ranging from 1.9 to 24% were generated by varying the input titer of wild-type HSV type 1 strain 17syn+. Reactivation of the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6888-6892.1998
更新日期:1998-08-01 00:00:00
abstract:UNLABELLED:Pneumonia virus of mice (PVM) is a natural rodent pathogen that replicates in bronchial epithelial cells and reproduces many clinical and pathological features of the more severe forms of disease associated with human respiratory syncytial virus. In order to track virus-target cell interactions during acute ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
abstract::Three of thirty-six monoclonal antibodies were found to cause irreversible inactivation of type 1 poliovirus at 39 degrees C but not at 37 degrees C. Neutralization at 37 degrees C depended on aggregation and was reversible by acid-induced deaggregation; at 39 degrees C, the virions (N antigenic, 160S) were disrupted ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5299-5302.1993
更新日期:1993-09-01 00:00:00
abstract::The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-05
更新日期:2006-07-01 00:00:00
abstract::9-([2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) is more potent and has a more prolonged inhibitory effect against Epstein-Barr virus (EBV) in vitro than does acyclovir (ACV). To assess the mechanism of this difference, we first compared the extent of phosphorylation of the two drugs in superinfected Ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.569-573.1986
更新日期:1986-11-01 00:00:00
abstract::Rotavirus NSP1 has been shown to function as an E3 ubiquitin ligase that mediates proteasome-dependent degradation of interferon (IFN) regulatory factors (IRF), including IRF3, -5, and -7, and suppresses the cellular type I IFN response. However, the effect of rotavirus NSP1 on viral replication is not well defined. P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00585-09
更新日期:2009-07-01 00:00:00
abstract::Large numbers of polymorphonuclear leukocytes (PMNs) infiltrated the murine vaginal mucosa within 24 h after intravaginal inoculation with an attenuated strain of herpes simplex virus type 2 (HSV-2). The role of these cells in resolution of a primary genital infection and in protection of HSV-immune animals against ch...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.6380-6386.1999
更新日期:1999-08-01 00:00:00
abstract::Comparative surface feature analyses of the VP1 sequences of hepatitis A virus (HAV) and poliovirus type 1 allowed an alignment of the two sequences and an identification of probable HAV neutralization antigenic sites. A synthetic peptide containing the HAV-specific amino acid sequence of one of these sites induced an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.3.836-839.1985
更新日期:1985-09-01 00:00:00
abstract::Following its entry into cells, pseudorabies virus (PRV) utilizes microtubules to deliver its nucleocapsid to the nucleus. Previous studies have shown that PRV VP1/2 is an effector of dynein-mediated capsid transport. However, the mechanism of PRV for recruiting microtubule motor proteins for successful neuroinvasion ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02052-18
更新日期:2019-03-21 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent involved in the disease HTLV-1-associated myelopathy, or tropical spastic paraparesis (HAM/TSP). The pathogenesis of HAM/TSP is poorly understood, but it is probable that viral infection has an indirect, deleterious effect on neural function. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
更新日期:1996-12-01 00:00:00
abstract::The size of the product RNA synthesized by the poliovirus RNA polymerase and host factor was significantly affected by the type of column chromatography used to purify the polymerase. Dimer length product RNA was synthesized by the polymerase purified by chromatography on hydroxylapatite. This contrasted with the mono...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.611-614.1987
更新日期:1987-02-01 00:00:00
abstract::Protocols have been established for the preparation of large amounts of pure measles virus intracellular nucleocapsids. As a result, it has been possible to routinely achieve nucleocapsid RNA yields of approximately 200 micrograms (from approximately 5 X 10(8) infected cells). Electrophoretic analysis of this RNA unde...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.57-65.1984
更新日期:1984-01-01 00:00:00
abstract::Plasmid lambdadv1, which is in a dimeric form, was converted to a linear monomer duplex by the action of EcoRI restriction endonuclease that incises at a unique site in this plasmid genome. The resulting products were then joined by Escherichia coli DNA ligase to produce molecules with various oligomeric forms, and fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.3.479-485.1975
更新日期:1975-09-01 00:00:00
abstract:UNLABELLED:Respiratory syncytial virus (RSV), a member of the Paramyxoviridae family of nonsegmented, negative-sense, single-stranded RNA genome viruses, is a leading cause of lower respiratory tract infections in infants, young children, and the elderly or immunocompromised. There are many open questions regarding the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00256-14
更新日期:2014-07-01 00:00:00
abstract::The mutational alterations in polyoma virus mutants ts-a and ts-25E which cause their large T-antigens to be thermolabile have been identified. In ts-a, a G leads to A transition at nucleotide 2193 causes the replacement of Ala (GCT) by Thr (ACT). In ts-25E, a G leads to T transversion at nucleotide 2883 causes the re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1094-1098.1981
更新日期:1981-03-01 00:00:00
abstract::The L5R gene of vaccinia virus is conserved among all sequenced members of the Poxviridae but has no predicted function or recognized nonpoxvirus homolog. Here we provide the initial characterization of the L5 protein. L5 is expressed following DNA replication with kinetics typical of a viral late protein, contains a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10988-10998.2005
更新日期:2005-09-01 00:00:00
abstract::Simian immunodeficiency virus (SIV) infection of natural hosts is characterized by nonpathogenic chronic viremia, maintenance of gastrointestinal epithelial barrier integrity, and low numbers of target cells. Assessment of cell-associated virus load in T cell subsets in multiple anatomic compartments of chronically SI...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01556-12
更新日期:2012-10-01 00:00:00
abstract::Kilham rat virus (KRV) contains linear, single-stranded DNA in the virion. The fate of radioactive viral DNA was followed after infection of monolayer cells. Within 60 min after infection of cells, 28 to 42% of the parental viral DNA is converted to a new form. This new DNA form is believed to be double stranded and l...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.2.299-305.1973
更新日期:1973-02-01 00:00:00