Abstract:
:In an effort to develop a useful AIDS vaccine or vaccine component, we have generated a combinatorial library of chimeric viruses in which the sequence IGPGRAFYTTKN from the V3 loop of the MN strain of human immunodeficiency virus type 1 (HIV-1) is displayed in many conformations on the surface of human rhinovirus 14 (HRV14). The V3 loop sequence was inserted into a naturally immunogenic site of the cold-causing HRV14, bridged by linkers consisting of zero to three randomized amino acids on each side. The library of chimeric viruses obtained was subjected to a variety of immunoselection schemes to isolate viruses that provided the most useful presentations of the V3 loop sequence for potential use in a vaccine against HIV. The utility of the presentations was assessed by measures of antigenicity and immunogenicity. Most of the immunoselected chimeras examined were potently neutralized by each of the four different monoclonal anti-V3 loop antibodies tested. Seven of eight chimeric viruses were able to elicit neutralizing antibody responses in guinea pigs against the MN and ALA-1 strains of HIV-1. Three of the chimeras elicited HIV neutralization titers that exceeded those of all but a small number of previously described HIV immunogens. These results indicate that HRV14:HIV-1 chimeras may serve as useful immunogens for stimulating immunity against HIV-1. This method can be used to flexibly reconstruct varied immunogens on the surface of a safe and immunogenic vaccine vehicle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Smith AD,Geisler SC,Chen AA,Resnick DA,Roy BM,Lewi PJ,Arnold E,Arnold GFdoi
10.1128/JVI.72.1.651-659.1998subject
Has Abstractpub_date
1998-01-01 00:00:00pages
651-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Herpes simplex virus (HSV) virion host shutoff protein (vhs) destabilizes cellular and viral mRNAs. Previous work from several laboratories has indicated that vhs accelerates the turnover of most host mRNAs and provided evidence that at least some of these are degraded via endonucleolytic cleavage near regions of tran...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.7.4090-4098.2005
更新日期:2005-04-01 00:00:00
abstract::Using coimmunoprecipitation and glutathione S-transferase pulldown experiments, we found that polyomavirus large T antigen binds to p300 in vivo and in vitro. The N-terminal region of the viral protein, including the pRB binding motif, was dispensable for this interaction, which involved several regions within the C-t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1734-1739.1999
更新日期:1999-02-01 00:00:00
abstract::The adenovirus tripartite leader is a 200-nucleotide 5' noncoding region that is found on all late viral mRNAs. This segment is required for preferential translation of viral mRNAs at late times during infection. Most tripartite leader-containing mRNAs appear to exhibit little if any requirement for intact cap-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2059-2066.1988
更新日期:1988-06-01 00:00:00
abstract::Early in infection by bacteriophage T4, before replication has commenced, one can detect the presence of newly synthesized DNA which cosediments with parental phage DNA on sucrose gradients. As shown earlier (R. E. Murray and C. K. Mathews, 1969), some of this represents covalent attachment of new material to parental...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.4.847-857.1974
更新日期:1974-04-01 00:00:00
abstract::Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2182-2191.1987
更新日期:1987-07-01 00:00:00
abstract::Co-infection of neonatal BALB/c mice with Friend virus (FV) complex (containing defective spleen focus-forming virus [SFFV] and endogenous N-tropic leukemia-inducing helper virus [LLV-F]) and B-tropic Tennant leukemia virus (TenLV) resulted in the inhibition of LLV-F by the Fv-1(b) gene and recovery of a TenLV pseudot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.523-533.1973
更新日期:1973-09-01 00:00:00
abstract::Infection of humans with wild-type measles virus leads to strong immune suppression and secondary infections, whereas immunization with an attenuated vaccine strain does not. Using the cotton rat model (Sigmodon hispidus), we investigated whether vaccine and wild-type viruses differ in viral spread and whether this is...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.150-158.2003
更新日期:2003-01-01 00:00:00
abstract::Latent HIV proviruses are silenced as the result of deacetylation and methylation of histones located at the viral long terminal repeat (LTR). Inhibition of histone deacetylases (HDACs) leads to the reemergence of HIV-1 from latency, but the contribution of histone lysine methyltransferases (HKMTs) to maintaining HIV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00836-11
更新日期:2011-09-01 00:00:00
abstract::We analyzed the susceptibility of 10 AKXD recombinant inbred (RI) mouse strains to lymphomas. These strains were derived from crosses of AKR/J, a highly lymphomatous strain, and DBA/2J, a weakly lymphomatous strain. Of the 10 strains analyzed, nine showed a high incidence of lymphoma development. As with the other 13 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2083-2090.1993
更新日期:1993-04-01 00:00:00
abstract::Adeno-associated virus (AAV) codes for four related nonstructural Rep proteins. AAV both replicates and assembles in the nucleus and requires coinfection with a helper virus, either adenovirus (Ad) or herpesvirus, for a productive infection. Like other more complex DNA viruses, it is believed that AAV interacts or mod...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01939-08
更新日期:2009-01-01 00:00:00
abstract::Transient transfection experiments indicated (i) that E6 protein of non-cancer-associated cutaneous human papillomavirus type 1 (HPV-1) can inhibit p53-mediated transcriptional transactivation in both p53-deficient human cells (H358) and normal rat cells (3Y1), but those of cancer-associated cutaneous HPV-5, -8, and -...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.7.4656-4661.1994
更新日期:1994-07-01 00:00:00
abstract::Latency is an integral feature of the pathogenesis of cytomegalovirus infection and disease. Using in situ hybridization, we detected viral DNA in the splenic stroma of mice with acute murine cytomegalovirus (MCMV) infection but could not detect latent infection. By using enzymatic amplification of a 700-bp region of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.6.3330-3334.1991
更新日期:1991-06-01 00:00:00
abstract::The hemagglutinin (HA), a glycoprotein on the surface of influenza A virus (IAV), initiates the virus life cycle by binding to terminal sialic acid (SA) residues on host cells. The HA gradually accumulates amino acid substitutions that allow IAV to escape immunity through a mechanism known as antigenic drift. We recen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01413-18
更新日期:2019-01-04 00:00:00
abstract::The Friend or Moloney mink cell focus-forming (MCF) virus encodes a recombinant-type envelope glycoprotein, gp70, that is closely related to the membrane glycoprotein, gp55, of Friend spleen focus-forming virus (SFFV). We have shown previously that gp55 has the ability to activate cell growth by binding to the cellula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.5.2408-2414.1991
更新日期:1991-05-01 00:00:00
abstract::The Vpu protein is a human immunodeficiency virus type 1 (HIV-1)-specific accessory protein that is required for the efficient release of viral particles from infected cells. Even though HIV-2 does not encode Vpu, we found that this virus is nevertheless capable of efficiently releasing virus particles. In fact, the r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.820-829.1996
更新日期:1996-02-01 00:00:00
abstract::The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::Human papillomavirus (HPV) is a strongly conserved DNA virus, high-risk types of which can cause cervical cancer in persistent infections. The most common type found in HPV-attributable cancer is HPV16, which can be subdivided into four lineages (A to D) with different carcinogenic properties. Studies have shown HPV16...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00844-17
更新日期:2017-09-12 00:00:00
abstract::Nucleocapsids were isolated from purified enveloped nucleocapsids of Plodia interpunctella granulosis virus by treatment with Nonidet P-40. When analyzed on sodium dodecyl sulfate-polyacrylamide gels, the nucleocapsids consisted of eight polypeptides. One of these, a major component with a molecular weight of 12,500 (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.2.866-876.1980
更新日期:1980-02-01 00:00:00
abstract::Phage XP-12, which has complete substitution of the cytosine residues in its DNA with 5-methylcytosine residues, was shown to inhibit incorporation of uracil into host DNA and RNA during the latent period. This apparent inhibition of host macromolecular synthesis was not accompanied by extensive degradation of the hos...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.517-523.1977
更新日期:1977-09-01 00:00:00
abstract::To combat emerging coronaviruses, developing safe and efficient platforms to evaluate viral protease activities and the efficacy of protease inhibitors is a high priority. Here, we exploit a biosafety level 2 (BSL-2) chimeric Sindbis virus system to evaluate protease activities and the efficacy of inhibitors directed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01749-14
更新日期:2014-10-01 00:00:00
abstract::T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10651-10657.2003
更新日期:2003-10-01 00:00:00
abstract::Knowledge of the mechanisms of virus dissemination in acute measles is cursory, but cells of the monocyte/macrophage (MM) lineage appear to be early targets. We characterized the dissemination of the Edmonston B vaccine strain of measles virus (MV-Ed) in peripheral blood mononuclear cells (PBMC) of two mouse strains e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.7.3343-3351.2001
更新日期:2001-04-01 00:00:00
abstract::The DNA product of the endogenously instructed RNA-dependent DNA polymerase reaction of murine sarcoma virus continued to be synthesized for as long as 64 h in the presence of 0.008% Triton X-100. Higher detergent concentrations and actinomycin D inhibited DNA product synthesis. The DNA product from long-term polymera...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.5.1049-1064.1973
更新日期:1973-11-01 00:00:00
abstract::Infection of neonatal mice with lymphocytic choriomeningitis virus (LCMV) strain Armstrong (ARM) results in a lifelong persistent infection. Viral variants (cytotoxic T lymphocyte [CTL] negative, persistence positive [CTL- P+]) can be isolated from the lymphoid tissues of such mice. Adult mice inoculated with these CT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7367-7373.1994
更新日期:1994-11-01 00:00:00
abstract::A human cytomegalovirus (CMV) UL54 pol exonuclease domain II mutation, D413A, found in a clinical specimen, conferred ganciclovir (GCV) and cidofovir resistance but not foscarnet resistance when incorporated into laboratory strain T2294. After several passages without drug, mutation was observed in five of eight plaqu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01787-07
更新日期:2008-01-01 00:00:00
abstract:UNLABELLED:Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00269-14
更新日期:2014-07-01 00:00:00
abstract::In earlier studies, we have shown that superinfection of an interleukin-2 (IL-2)-dependent, Moloney murine leukemia virus (MoMuLV)-induced rat T-cell lymphoma line (4437A) with mink cell focus-forming (also called polytropic) murine retroviruses induces rapid progression to IL-2-independent growth. In this report, we ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.7709-7716.1994
更新日期:1994-12-01 00:00:00
abstract::We have previously described several human immunodeficiency virus type 1 (HIV-1) mutants that are characterized by an excessive-RNA-splicing phenotype and reduced virus particle production. In one of these mutants (NLD2up), the sequence of 5' splice site D2 was changed to a consensus splice donor site. This splice sit...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02295-07
更新日期:2008-02-01 00:00:00
abstract::A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly complete viral genome, but others have sustained deletions of 1 to 2 kilobases in the polymerase gene. The pattern of rel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.914-924.1982
更新日期:1982-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a cluster of 12 microRNAs (miRNAs) that are processed from a transcript that is embedded within the major latency control region. We have generated a deletion mutation that eliminates 10 of the 12 viral miRNAs from the KSHV bacmid by using recombineering methods. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-09
更新日期:2010-03-01 00:00:00