Abstract:
:In earlier studies, we have shown that superinfection of an interleukin-2 (IL-2)-dependent, Moloney murine leukemia virus (MoMuLV)-induced rat T-cell lymphoma line (4437A) with mink cell focus-forming (also called polytropic) murine retroviruses induces rapid progression to IL-2-independent growth. In this report, we present evidence that the vast majority (> 90%) of the IL-2-independent lines established from polytropic or xenotropic virus-infected 4437A cells carry provirus insertions in the 3' untranslated region of the IL-9 receptor gene (Gfi-2 [for growth factor independence-2]/IL-9R). Prior to superinfection, the cells express neither IL-9 nor IL-9R. Following superinfection and provirus insertion in the Gfi-2/IL-9R locus, the cells express high levels of mRNA transcripts with a truncated 3' untranslated region which are predicted to encode the normal IL-9R protein product. The same IL-2-independent cells also express IL-9 which is induced by an insertional mutagenesis-independent mechanism. The establishment of an IL-9-dependent autocrine loop was sufficient to render the cells IL-2 independent, as suggested by the finding that 4437A cells, expressing a stably transfected Gfi-2/IL-9R construct, do not require IL-2 when maintained in IL-9-containing media. Additional experiments designed on the basis of these results showed that IL-9 gene expression is induced rapidly following the infection of 4437A cells by polytropic or xenotropic viruses and occurs in the absence of selection for IL-2-independent growth. Taken together, these data suggest that infection of 4437A cells by mink cell focus-forming or xenotropic viruses induces the expression of IL-9, which in turn rapidly selects the cells expressing the IL-9 receptor through an insertional mutagenesis-dependent mechanism. Given that both the polytropic and xenotropic viruses can induce the IL-9-dependent autocrine loop, the reduced ability of the xenotropic viruses to rapidly induce IL-2 independence in culture and tumors in animals is likely to be the result of their lower growth rates.
journal_name
J Viroljournal_title
Journal of virologyauthors
Flubacher MM,Bear SE,Tsichlis PNdoi
10.1128/JVI.68.12.7709-7716.1994subject
Has Abstractpub_date
1994-12-01 00:00:00pages
7709-16issue
12eissn
0022-538Xissn
1098-5514journal_volume
68pub_type
杂志文章abstract::Inbred LSH hamster brain cells were transformed in vitro by the GS strain of BK virus (BKV), and transplantable tumors classified as undifferentiated glioblastomas were induced in the syngeneic host. The viral status in the transformed cells, designated LSH-BR-BK, was established. About 46 genome equivalents per cell ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.1.276-284.1981
更新日期:1981-10-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is a cytoplasmic receptor/transcription factor that modulates several cellular and immunological processes following activation by pathogen-associated stimuli, though its role during virus infection is largely unknown. Here, we show that AhR is activated in cells infected with mouse...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01743-19
更新日期:2020-01-17 00:00:00
abstract::Neurotropic herpesviruses depend on long-distance axon transport for the initial establishment of latency in peripheral ganglia (retrograde transport) and for viral spread in axons to exposed body surfaces following reactivation (anterograde transport). Images of neurons infected with herpes simplex virus type 1 (HSV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01296-10
更新日期:2010-12-01 00:00:00
abstract::Synthetic peptides of increasing length and corresponding in sequence to the C-terminal end of the HA1 molecule of influenza virus were constructed and examined for their immunogenic and antigenic properties. Peptides containing at least the four C-terminal amino acids, when coupled to keyhole limpet hemocyanin, were ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4745-4751.1988
更新日期:1988-12-01 00:00:00
abstract::Human cytomegalovirus (HCMV) is a prevalent pathogen that establishes lifelong infection in the host. Virus persistence is aided by extensive manipulation of the host immune system, particularly cytokine and chemokine signaling pathways. The HCMV UL111A gene encodes cmvIL-10, an ortholog of human interleukin-10 that h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01981-17
更新日期:2018-02-12 00:00:00
abstract::The techniques of deoxyribonucleic acid-ribonucleic acid (DNA-RNA) hybridization and immunological precipitation were used to compare the synthesis of adenovirus-specific macromolecules in African green monkey kidney (AGMK) cells infected with adenovirus, an abortive infection, and coinfected with both adenovirus and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1109-1117.1972
更新日期:1972-12-01 00:00:00
abstract::Virus-specific proteins were examined in cultured cells infected with bovine viral diarrhea virus. By using antisera obtained from virus-infected animals, three major virus-specific polypeptides with molecular weights of 115,000 (115K), 80K, and 55K were observed. Minor proteins of 45,000 and 38,000 daltons were also ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.2.666-669.1984
更新日期:1984-05-01 00:00:00
abstract::The global eradication of poliomyelitis will require substantial changes in immunization practices. One of the proposed scenarios includes cessation of vaccination with live oral poliovirus vaccine (OPV) and the creation of an OPV stockpile for emergency response in case of the reintroduction of poliovirus into circul...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12460-12465.2003
更新日期:2003-12-01 00:00:00
abstract::Mouse neuroblastoma cells containing intracisternal type A particles were treated with iododeoxyuridine and dexamethasone to induce the release of type C oncornavirus particles. For 5 days after treatment, antigenic markers and DNA polymerase activities specific to particles of each of the two types were assayed in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.709-716.1976
更新日期:1976-08-01 00:00:00
abstract::The retrovirus restriction factor TRIM5alpha targets the viral capsid soon after entry. Here we show that the TRIM5alpha protein oligomerizes into trimers. The TRIM5alpha coiled-coil and B30.2(SPRY) domains make important contributions to the formation and/or stability of the trimers. A functionally defective TRIM5alp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14446-14450.2005
更新日期:2005-11-01 00:00:00
abstract::We previously demonstrated that chimeric porcine parvovirus-like particles (PPV:VLP) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong CD4(+) and CD8(+) T-cell responses specific for the foreign epitopes. In the present study, we investigated the immunogenicity...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.2739-2744.1999
更新日期:1999-04-01 00:00:00
abstract::Vesicular stomatitis virus Indiana strain G protein (VSVind.G) is the most commonly used envelope glycoprotein to pseudotype lentiviral vectors (LV) for experimental and clinical applications. Recently, G proteins derived from other vesiculoviruses (VesG), for example, Cocal virus, have been proposed as alternative LV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00900-18
更新日期:2018-11-12 00:00:00
abstract::Retroviral genomes are dimeric, comprised of two sense-strand RNAs linked at their 5' ends by noncovalent base pairing and tertiary interactions. Viral maturation involves large-scale morphological changes in viral proteins and in genomic RNA dimer structures to yield infectious virions. Structural studies have largel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01602-09
更新日期:2010-01-01 00:00:00
abstract::A mutant of herpes simplex virus type 1 (HSV-1) in which glycoprotein H (gH) coding sequences were deleted and replaced by the Escherichia coli lacZ gene under the control of the human cytomegalovirus IE-1 gene promoter was constructed. The mutant was propagated in Vero cells which contained multiple copies of the HSV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.341-348.1992
更新日期:1992-01-01 00:00:00
abstract::Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2449-2459.1996
更新日期:1996-04-01 00:00:00
abstract:UNLABELLED:Replication-deficient rabies virus (RABV)-based vaccines induce rapid and potent antibody responses via T cell-independent and T cell-dependent mechanisms. To further investigate early events in vaccine-induced antibody responses against RABV infections, we studied the role of macrophages as mediators of RAB...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03409-14
更新日期:2015-03-01 00:00:00
abstract::This paper describes Q beta noninfectious particles produced at 41 degrees C in a streptomycin-resistant Escherichia coli mutant which is temperature sensitive for suppression of a nonsense codon. The noninfectious particles resembled Q beta under the electron microscope and contained coat protein molecules in an amou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.1107-1117.1979
更新日期:1979-03-01 00:00:00
abstract::Human papillomavirus type 11 (HPV-11) and HPV-16 contain an E5 gene that can induce c-fos gene expression in mouse fibroblasts. This study investigated the human c-fos promoter characteristics by mapping the c-fos promoter sequence with several deletion and point mutants that confer responsiveness to E5 of HPV-11 or H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8558-8563.1996
更新日期:1996-12-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract::Many of the approximately 165 proteins encoded by the African swine fever virus (ASFV) genome do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times postinfection. Deletion of this gene did ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01428-17
更新日期:2017-11-30 00:00:00
abstract::The development of immunization strategies to protect against ocular infection with herpes simplex virus 1 (HSV-1) must address the issue of the effects of the strategy on the establishment of latency in the trigeminal ganglia (TG). It is the reactivation of this latent virus that can cause recurrent disease and corne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00913-14
更新日期:2014-07-01 00:00:00
abstract::The hepatitis C virus (HCV) encodes a large polyprotein; therefore, all viral proteins are produced in equimolar amounts regardless of their function. The aim of our study was to determine the ratio of nonstructural proteins to RNA that is required for HCV RNA replication. We analyzed Huh-7 cells harboring full-length...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13594-13605.2005
更新日期:2005-11-01 00:00:00
abstract:UNLABELLED:Bluetongue is one of the major infectious diseases of ruminants and is caused by bluetongue virus (BTV), an arbovirus existing in nature in at least 26 distinct serotypes. Here, we describe the development of a vaccine platform for BTV. The advent of synthetic biology approaches and the development of revers...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02183-14
更新日期:2014-11-01 00:00:00
abstract::Extracts of competent cells of Bacillus subtilis exhibited nuclease activity on radioactively labeled cell deoxyribonucleic acid (DNA). The activity was not decreased when Mg was omitted from the reaction mixture but was decreased to zero by addition of ethylenediaminetetraacetic acid. Of the other metals tested, only...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.749-752.1971
更新日期:1971-06-01 00:00:00
abstract:UNLABELLED:Lethal mutagenesis is a broad-spectrum antiviral strategy that exploits the high mutation rate and low mutational tolerance of many RNA viruses. This approach uses mutagenic drugs to increase viral mutation rates and burden viral populations with mutations that reduce the number of infectious progeny. We inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03483-14
更新日期:2015-04-01 00:00:00
abstract::The glucocorticoid-regulatory sequences from the murine mammary tumor virus long terminal repeat (MMTV LTR) were introduced into the LTR of Moloney murine leukemia virus (M-MuLV) by recombinant DNA techniques. The site of insertion was in the M-MuLV LTR U3 region at -150 base pairs with respect to the RNA cap site. In...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.133-144.1985
更新日期:1985-04-01 00:00:00
abstract::The large T antigen encoded by simian virus 40 (SV40) plays essential roles in the infection of permissive cells, leading to production of progeny virions, and in the infection of nonpermissive cells, leading to malignant transformation. Primary mouse embryo fibroblasts (MEFs) are nonpermissive for SV40, and infection...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.12.6872-6880.1991
更新日期:1991-12-01 00:00:00
abstract:UNLABELLED:Influenza A virus requires ongoing cellular transcription to carry out the cap-snatching process. Chromatin remodelers modify chromatin structure to produce an active or inactive conformation, which enables or prevents the recruitment of transcriptional complexes to specific genes; viral transcription thus d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00053-16
更新日期:2016-01-20 00:00:00
abstract::Nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) possesses an RNA-dependent RNA polymerase activity responsible for viral genome RNA replication. Despite several reports on the characterization of this essential viral enzyme, little is known about the reaction pathway of NS5B-catalyzed nucleotide incorporati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.19.9134-9143.2000
更新日期:2000-10-01 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent involved in the disease HTLV-1-associated myelopathy, or tropical spastic paraparesis (HAM/TSP). The pathogenesis of HAM/TSP is poorly understood, but it is probable that viral infection has an indirect, deleterious effect on neural function. In ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
更新日期:1996-12-01 00:00:00