Abstract:
:Many of the approximately 165 proteins encoded by the African swine fever virus (ASFV) genome do not have significant similarity to known proteins and have not been studied experimentally. One such protein is DP148R. We showed that the DP148R gene is transcribed at early times postinfection. Deletion of this gene did not reduce virus replication in macrophages, showing that it is not essential for replication in these cells. However, deletion of this gene from a virulent isolate, Benin 97/1, producing the BeninΔDP148R virus, dramatically reduced the virulence of the virus in vivo All pigs infected with the BeninΔDP148R virus survived infection, showing only transient mild clinical signs soon after immunization. Following challenge with the parental virulent virus, all pigs immunized by the intramuscular route (11/11) and all except one immunized by the intranasal route (5/6) survived. Mild or no clinical signs were observed after challenge. As expected, control nonimmune pigs developed signs of acute African swine fever (ASF). The virus genome and infectious virus were observed soon after immunization, coincident with the onset of clinical signs (∼106 genome copies or 50% tissue culture infective doses/ml). The levels of the virus genome declined over an extended period up to 60 days postimmunization. In contrast, infectious virus was no longer detectable by days 30 to 35. Gamma interferon (IFN-γ) was detected in serum between days 4 and 7 postimmunization, and IFN-γ-producing cells were detected in all pigs analyzed following stimulation of immune lymphocytes with whole virus. ASFV-specific antibodies were first detected from day 10 postimmunization.IMPORTANCE African swine fever (ASF) is endemic in Africa, parts of the Trans Caucasus, the Russian Federation, and several European countries. The lack of a vaccine hinders control. Many of the ASF virus genes lack similarity to known genes and have not been characterized. We have shown that one of these, DP148R, is transcribed early during virus replication in cells and can be deleted from the virus genome without reducing virus replication. The virus with the gene deletion, BeninΔDP148R, caused mild clinical signs in pigs and induced high levels of protection against challenge with the parental virulent virus. Therefore, deletion of this gene can provide a target for the rational development of vaccines.
journal_name
J Viroljournal_title
Journal of virologyauthors
Reis AL,Goatley LC,Jabbar T,Sanchez-Cordon PJ,Netherton CL,Chapman DAG,Dixon LKdoi
10.1128/JVI.01428-17subject
Has Abstractpub_date
2017-11-30 00:00:00issue
24eissn
0022-538Xissn
1098-5514pii
JVI.01428-17journal_volume
91pub_type
杂志文章abstract::The rat-derived Harvey murine sarcoma virus (Ha-MuSV) contains a transduced ras oncogene activated by two missense mutations and flanked by rat retroviruslike VL30 sequences. Ha-MuSV induces focal transformation of mouse NIH 3T3 cells in vitro and tumors (fibrosarcomas and splenic erythroleukemias) in newborn mice. We...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1384-1392.1989
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.61.9.2793-2799.1987
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pub_type: 杂志文章
doi:10.1128/JVI.18.3.1143-1146.1976
更新日期:1976-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.01287-09
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.2.397-400.1973
更新日期:1973-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.3.516-523.1972
更新日期:1972-09-01 00:00:00
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更新日期:2005-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.8.4103-4107.2002
更新日期:2002-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) nucleotide sequences encoding p24Gag and the Env C2V3 region were obtained from seven patients who were selected on the basis of having paradoxical seronegativity on a subset of HIV enzyme-linked immunosorbent assay detection kits and having atypical Western blot (immunoblot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.9.5640-5649.1995
更新日期:1995-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2020-08-31 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:1968-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8727-8736.1996
更新日期:1996-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-12-16 00:00:00
abstract::Myxoma virus (MV) encodes a cell surface protein (M135R) that is predicted to mimic the host alpha/beta interferon receptor (IFN-alpha/beta-R) and thus prevent IFN-alpha/beta from triggering a host antiviral response. This prediction is based on sequence similarity to B18R, the viral IFN-alpha/beta-R from vaccinia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01633-06
更新日期:2007-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5520-5526.1999
更新日期:1999-07-01 00:00:00