Abstract:
:A mutant of herpes simplex virus type 1 (HSV-1) in which glycoprotein H (gH) coding sequences were deleted and replaced by the Escherichia coli lacZ gene under the control of the human cytomegalovirus IE-1 gene promoter was constructed. The mutant was propagated in Vero cells which contained multiple copies of the HSV-1 gH gene under the control of the HSV-1 gD promoter and which therefore provide gH in trans following HSV-1 infection. Phenotypically gH-negative virions were obtained by a single growth cycle in Vero cells. These virions were noninfectious, as judged by plaque assay and by expression of beta-galactosidase following high-multiplicity infection, but partial recovery of infectivity was achieved by using the fusogenic agent polyethylene glycol. Adsorption of gH-negative virions to cells blocked the adsorption of superinfecting wild-type virus, a result in contrast to that obtained with gD-negative virions (D. C. Johnson and M. W. Ligas, J. Virol. 62:4605-4612, 1988). The simplest conclusion is that gH is required for membrane fusion but not for receptor binding, a conclusion consistent with the conservation of gH in all herpesviruses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Forrester A,Farrell H,Wilkinson G,Kaye J,Davis-Poynter N,Minson Tdoi
10.1128/JVI.66.1.341-348.1992subject
Has Abstractpub_date
1992-01-01 00:00:00pages
341-8issue
1eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::The DNA synthesized in the nonpermissive host by the noncomplementing mutants am36 and am42 of bacteriophage phi W-14 contains about half the wild-type level of alpha-putrescinylthymine (putThy) and a correspondingly greater level of thymine. The mechanisms whereby thymine nucleotides are excluded from replicating DNA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.3.399-405.1983
更新日期:1983-09-01 00:00:00
abstract::Several viruses, including members of the gammaherpesvirus family, encode proteins that are secreted into the extracellular environment. We have identified an abundant 44-kDa secreted protein that is present in the supernatant of fibroblasts infected with murine gammaherpesvirus 68 (gammaHV68; also referred to as MHV-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4524-4529.1999
更新日期:1999-05-01 00:00:00
abstract:UNLABELLED:Although the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) epidemic was controlled by nonvaccine measures, coronaviruses remain a major threat to human health. The design of optimal coronavirus vaccines therefore remains a priority. Such vaccines present major challenges: coronavirus im...
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pub_type: 杂志文章
doi:10.1128/JVI.02980-14
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00870-14
更新日期:2014-09-01 00:00:00
abstract:UNLABELLED:Reactivation of human cytomegalovirus (HCMV) is a significant cause of disease and death in immunocompromised patients, underscoring the need to understand how latency is controlled. Here we demonstrate that HCMV has evolved to utilize cellular microRNAs (miRNAs) in cells that promote latency to regulate exp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00481-14
更新日期:2014-05-01 00:00:00
abstract::The signaling lymphocyte activation molecule F1 (SLAMF1) is both a microbial sensor and entry receptor for measles virus (MeV). Herein, we describe a new role for SLAMF1 to mediate MeV endocytosis that is in contrast with the alternative, and generally accepted, model that MeV genome enters cells only after fusion at ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02255-16
更新日期:2017-03-13 00:00:00
abstract::The replication proteins Rep and Rep' of porcine circovirus type 1 (PCV1) are both capable of introducing and resealing strand discontinuities at the viral origin of DNA replication in vitro underlying genome amplification by rolling-circle replication. The PCV1 origin of replication encompasses the minimal binding si...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02420-06
更新日期:2007-06-01 00:00:00
abstract::Type 3 reovirus inhibits L cell DNA synthesis, whereas type 1 reovirus exerts little or no effect on L cell DNA synthesis. By using recombinant viruses containing both type 1 and type 3 double-standard RNA segments, we determined that one double-stranded RNA segment, the reovirus type 3 S1 double-stranded RNA segment ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.1.389-392.1981
更新日期:1981-04-01 00:00:00
abstract::A gene encoding a polypeptide with homology to mammalian vascular endothelial growth factors (VEGFs) has been discovered in the genome of orf virus (OV), a parapoxvirus that affects sheep and goats and, occasionally, humans. The gene is transcribed abundantly early in infection and is found immediately outside the inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.84-92.1994
更新日期:1994-01-01 00:00:00
abstract::The protein product of varicella-zoster virus (VZV) ORF47 is a serine/threonine protein kinase and tegument component. Evaluation of two recombinants of the Oka strain, rOka47DeltaC, with a C-terminal truncation of ORF47, and rOka47D-N, with a point mutation in the conserved kinase motif, showed that ORF47 kinase func...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.13293-13305.2004
更新日期:2004-12-01 00:00:00
abstract::Despite intensive study, the mechanism by which many retroviruses complete reverse transcription has remained unclear. Most retroviruses and all lentiviruses fail to synthesize a full-length second strand of the viral cDNA (plus strand) efficiently in infected cells. For human immunodeficiency virus type 1, we find in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3938-3944.1995
更新日期:1995-06-01 00:00:00
abstract::Five human recombinant Fab fragments (Fabs) specific for measles virus (MV) proteins were isolated from three antibody phage display libraries generated from RNAs derived from bone marrow or splenic lymphocytes from three MV-immune individuals. All Fabs reacted in an enzyme-linked immunosorbent assay with MV antigens....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.1.251-258.2002
更新日期:2002-01-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) genomes are associated with the repressive heterochromatic marks H3K9me2/me3 and H3K27me3 during latency. Previous studies have demonstrated that inhibitors of H3K9me2/me3 histone demethylases reduce the ability of HSV-1 to reactivate from latency. Here we demonstrate that GSK-J4, a spec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03052-14
更新日期:2015-03-01 00:00:00
abstract::Identification of cell surface viral binding proteins is important for understanding viral attachment and internalization. We have fused the pre-S domain of the duck hepatitis B virus (DHBV) large envelope protein to glutathione S-transferase and demonstrated a 170-kDa binding protein (p170) in [35S]methionine-labeled...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7106-7112.1995
更新日期:1995-11-01 00:00:00
abstract::We have developed a system for producing murine leukemia virus (MLV) pseudotyped with human hepatitis B virus (HBV) large (L) and small (S) surface antigens (HBsAg) for targeting primary human hepatocytes. Using the MLV(HBV) pseudotype virus containing a beta-galactosidase reporter gene, we demonstrated that this pseu...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/jvi.76.2.912-917.2002
更新日期:2002-01-01 00:00:00
abstract::Autophagy is now known to be an essential component of host innate and adaptive immunity. Several herpesviruses have developed various strategies to evade this antiviral host defense. Herpes simplex virus 1 (HSV-1) blocks autophagy in fibroblasts and in neurons, and the ICP34.5 protein is important for the resistance ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01158-12
更新日期:2013-01-01 00:00:00
abstract::MC159 is a viral FLIP (FLICE inhibitory protein) encoded by the molluscum contagiosum virus (MCV) enabling MCV to evade antiviral immunity and to establish persistent infections in humans. Here, we show that MC159 contains a functional SH3 binding motif, which mediates avid and selective binding to SH3BP4, a signaling...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01613-18
更新日期:2019-05-01 00:00:00
abstract::Treatment of polyoma virions with ethyleneglycol-bil-N,N'-tetraacetic acid (EGTA) and dithiothreitol (DTT) at pH 8.5 resulted in the dissociation of the virions into a DNA-protein complex and individual structural capsomere subunits. The sedimentation value of the DNA-protein complex in sucrose gradients was approxima...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.1.193-204.1978
更新日期:1978-07-01 00:00:00
abstract::Hepatitis C virus (HCV) infection causes not only intrahepatic diseases but also extrahepatic manifestations, including type 2 diabetes. We previously reported that HCV replication suppresses cellular glucose uptake by downregulation of cell surface expression of glucose transporter 2 (GLUT2) (D. Kasai et al., J. Hepa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01418-12
更新日期:2012-12-01 00:00:00
abstract::The simian-human immunodeficiency virus SHIV-HXBc2 contains the envelope glycoproteins of a laboratory-adapted, neutralization-sensitive human immunodeficiency virus type 1 variant, HXBc2. Serial in vivo passage of the nonpathogenic SHIV-HXBc2 generated SHIV KU-1, which causes rapid CD4(+) T-cell depletion and an AIDS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4208-4218.2001
更新日期:2001-05-01 00:00:00
abstract::Hepatitis C virus (HCV) is a major causative agent of posttransfusion non-A, non-B hepatitis, which often develops into malignant chronic diseases, including liver cirrhosis and hepatocellular carcinoma. We have cloned from human carriers overlapping cDNAs (9,416 bp) covering the entire coding region of the HCV genome...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1105-1113.1991
更新日期:1991-03-01 00:00:00
abstract::Feline leukemia viruses (FeLVs) belonging to interference subgroup C induce fatal anemia resembling human pure red cell aplasia (PRCA). Subgroup A FeLVs, although closely related genetically to FeLVs of subgroup C, do not induce PRCA. The determinants for PRCA induction by a molecularly cloned prototype subgroup C vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5561-5568.1992
更新日期:1992-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) LANA is an 1,162-amino-acid protein that tethers terminal repeat (TR) DNA to mitotic chromosomes to mediate episome persistence in dividing cells. C-terminal LANA self-associates to bind TR DNA. LANA contains independent N- and C-terminal chromosome binding regions. N-ter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02395-08
更新日期:2009-05-01 00:00:00
abstract::Polyclonal and monoclonal antibodies to individual herpes simplex virus (HSV) glycoproteins were tested for ability to inhibit adsorption of radiolabeled HSV type 1 (HSV-1) strain HFEMsyn [HSV-1(HFEM)syn] to HEp-2 cell monolayers. Polyclonal rabbit antibodies specific for glycoprotein D (gD) or gC and three monoclonal...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.55.2.475-482.1985
更新日期:1985-08-01 00:00:00
abstract::Parvoviruses infect humans and a broad range of animals, from mammals to crustaceans, and generally are associated with a variety of acute and chronic diseases. However, many others cause persistent infections and are not known to be associated with any disease. Viral persistence is likely related to the ability to in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00828-11
更新日期:2011-10-01 00:00:00
abstract::Northern (RNA) blot analysis was used to determine the spatial and temporal distribution of bovine herpesvirus 1 (BHV-1) transcripts. Total RNA was isolated from Madin-Darby bovine kidney cells which had been infected with BHV-1.2b strain K22 or BHV-1.1 strain Jura in the presence or absence of metabolic inhibitors. C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4882-4889.1989
更新日期:1989-11-01 00:00:00
abstract::Vaccine-elicited humoral immune responses comprise an array of antibody forms and specificities, with only a fraction contributing to protective host immunity. Elucidation of antibody effector functions responsible for protective immunity against human immunodeficiency virus type 1 (HIV-1) acquisition is a major goal ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01552-17
更新日期:2018-03-14 00:00:00
abstract::Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02298-10
更新日期:2011-07-01 00:00:00
abstract::The alternating host cycle and persistent vector infection may constrain the evolution of arboviruses. To test this hypothesis, eastern equine encephalitis virus was passaged in BHK or mosquito cells, as well as in alternating (both) host cell passages. High and low multiplicities were used to examine the effect of de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4316-4326.1999
更新日期:1999-05-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTL) play an important role in recovery from a number of viral infections. They are also implicated in virus-induced immunopathology as best demonstrated in lymphocytic choriomeningitis virus (LCMV) infection of adult immunocompetent mice. In the present study, the structure of the T-cell rece...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.12.5919-5926.1990
更新日期:1990-12-01 00:00:00