Abstract:
:The Vpu protein is a human immunodeficiency virus type 1 (HIV-1)-specific accessory protein that is required for the efficient release of viral particles from infected cells. Even though HIV-2 does not encode Vpu, we found that this virus is nevertheless capable of efficiently releasing virus particles. In fact, the rate of virus release from HeLa cells transfected with a full-length molecular clone of HIV-2, ROD10, was comparable to that observed for the vpu+ HIV-1 NL4-3 isolate and was not further enhanced by expression of Vpu in trans. However, consistent with previous observations showing that HIV-2 particle release is Vpu responsive in the context of HIV-1/HIV-2 chimeric constructs; exchanging the gag-pol region of NL4-3 with the corresponding region from pROD10 rendered the resulting chimeric virus Vpu responsive. Our finding that the responsiveness of HIV-2 particle release to Vpu is context dependent suggested the presence of a Vpu-like factor(s) encoded by HIV-2. Using chimeric proviruses encoding HIV-2 gag and pol in the context of the HIV-1 provirus that were coexpressed with subgenomic HIV-2 constructs, we found that the HIV-2 envelope glycoprotein had the ability to enhance HIV-2 particle release with an efficiency comparable to that of the HIV-1 Vpu protein. Conversely, inactivation of the HIV-2 env gene in the original ROD10 clone resulted in a decrease in the rate of viral particle release to a level that was comparable to that of Vpu-deficient HIV-1 isolates. Providing the wild-type envelope in trans rescued the particle release defect of the ROD10 envelope mutant. Thus, unlike HIV-1, which encodes two separate proteins to regulate virus release or to mediate viral entry, the HIV-2 Env protein has evolved to perform both functions.
journal_name
J Viroljournal_title
Journal of virologyauthors
Bour S,Schubert U,Peden K,Strebel Kdoi
10.1128/JVI.70.2.820-829.1996subject
Has Abstractpub_date
1996-02-01 00:00:00pages
820-9issue
2eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::The unexpectedly low efficacy of influenza vaccine during school outbreaks of influenza B virus in the spring of 1987 in Japan was probably attributable to a poor antibody response of vaccinees to the epidemic viruses. An antigenic analysis of the causative B viruses isolated in 1987 and 1988 showed much variation in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
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pub_type: 杂志文章
doi:10.1128/JVI.65.8.4130-4136.1991
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pub_type: 杂志文章
doi:10.1128/JVI.8.3.303-310.1971
更新日期:1971-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.02400-09
更新日期:2010-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9054-9060.1998
更新日期:1998-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.72.2.1060-1070.1998
更新日期:1998-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
更新日期:2007-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01646-07
更新日期:2007-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06282-11
更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.709-709.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02497-09
更新日期:2010-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.993-1000.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02237-08
更新日期:2009-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3706-3718.2001
更新日期:2001-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.62.8.2755-2761.1988
更新日期:1988-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.86-95.1976
更新日期:1976-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01040-16
更新日期:2016-08-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.18.8848-8853.2001
更新日期:2001-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.2.680-683.1986
更新日期:1986-05-01 00:00:00
abstract::ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10658-10669.2000
更新日期:2000-11-01 00:00:00
abstract::We previously reported that a mutant Rous sarcoma virus (RSV) with an alternate polypurine tract (PPT), DuckHepBFlipPPT, had unexpectedly high titers and that the PPT was miscleaved primarily at one position following a GA dinucleotide by the RNase H of reverse transcriptase (RT). This miscleavage resulted in a portio...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00