Abstract:
:Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time course and distribution of PC pathology have not been investigated rigorously. This study examined the spatiotemporal relationship between PC death and zonal organization in NBD cerebella. Real-time PCR at postnatal day 28 (PND28) revealed decreased cerebellar levels of mRNAs encoding the glycolytic enzymes aldolase C (AldoC, also known as zebrin II) and phosphofructokinase C and the excitatory amino acid transporter 4 (EAAT4). Zebrin II and EAAT4 immunofluorescence analysis in PND21, PND28, PND42, and PND84 NBD rat cerebella revealed a complex pattern of PC degeneration. Early cell loss (PND28) was characterized by preferential apoptotic loss of zebrin II/EAAT4-negative PC subsets in the anterior vermis. Consistent with early preferential loss of zebrin II/EAAT4-negative PCs in the vermis, the densities of microglia and the Bergmann glial expression of metallothionein I/II and the hyaluronan receptor CD44 were higher in zebrin II/EAAT4-negative zones. In contrast, early loss in lateral cerebellar lobules did not reflect a similar discrimination between PC phenotypes. Patterns of vermal PC loss became more heterogeneous at PND42, with the loss of both zebrin II/EAAT4-negative and zebrin II/EAAT4-positive neurons. At PND84, zebrin II/EAAT4 patterning was abolished in the anterior cerebellum, with preferential PC survival in lobule X. Our investigation reveals regional discrimination between patterns of PC subset loss, defined by zebrin II/EAAT4 expression domains, following neonatal viral infection. These findings suggest a differential vulnerability of PC subsets during the early stages of virus-induced neurodegeneration.
journal_name
J Viroljournal_title
Journal of virologyauthors
Williams BL,Yaddanapudi K,Hornig M,Lipkin WIdoi
10.1128/JVI.02245-06subject
Has Abstractpub_date
2007-03-01 00:00:00pages
2675-87issue
6eissn
0022-538Xissn
1098-5514pii
JVI.02245-06journal_volume
81pub_type
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.4968-4977.1990
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journal_title:Journal of virology
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doi:10.1128/jvi.76.14.7103-7113.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.1110-1121.1993
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doi:10.1128/jvi.74.6.2558-2566.2000
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journal_title:Journal of virology
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doi:10.1128/JVI.71.1.299-306.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8910-8919.2002
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pub_type: 杂志文章
doi:10.1128/JVI.02395-08
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.8.7008-7013.1999
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.2967-2972.2000
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.688-692.1986
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.213-221.1993
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1103-1114.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8718-8725.1997
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4729-4736.1996
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.17.8702-8709.2002
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.2.626-635.1977
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.903-913.1981
更新日期:1981-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7178-7187.1994
更新日期:1994-11-01 00:00:00