Abstract:
:Primary human embryonic kidney (HEK) cells were transformed by a focus assay with BK virus (BKV) DNA molecularly cloned at its unique EcoRI site. Both viral DNA sequences and viral tumor antigens were present and expressed in all the foci that we examined. However, cells isolated from foci were incapable of growth in soft agar. We then examined the transformation of HEK cells after their transfection with a combination of BKV DNA and either the normal or the activated form of the human Ha-ras oncogene (EJ c-Ha-ras-1). Only the cells transfected with a combination of BKV DNA and the activated form of Ha-ras were capable of growth in soft agar. Both BKV and Ha-ras DNAs were present in the transformed colonies. BKV tumor antigens and the Ha-ras p21 protein were also expressed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Pater A,Pater MMdoi
10.1128/JVI.58.2.680-683.1986subject
Has Abstractpub_date
1986-05-01 00:00:00pages
680-3issue
2eissn
0022-538Xissn
1098-5514journal_volume
58pub_type
杂志文章abstract::A systematic analysis of immune responses on a population level is critical for a human immunodeficiency virus type 1 (HIV-1) vaccine design. Our studies in Botswana on (i) molecular analysis of the HIV-1 subtype C (HIV-1C) epidemic, (ii) frequencies of major histocompatibility complex class I HLA types, and (iii) cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10155-10168.2002
更新日期:2002-10-01 00:00:00
abstract::A contiguously linked T-B synthetic viral epitope (110HA120-150HA159,T-B) was investigated for its potency in inducing humoral and cellular immune responses in vivo. The T-cell epitope 110HA120 corresponds to the site 1 hemagglutinin (HA) of the A/PR/8/34 (PR8) influenza virus and is recognized by CD4 T cells in assoc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5473-5480.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6788-6793.1992
更新日期:1992-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06324-11
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.5.1046-1054.1974
更新日期:1974-05-01 00:00:00
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journal_title:Journal of virology
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abstract::Using isogenic recombinant murine coronaviruses expressing wild-type murine hepatitis virus strain 4 (MHV-4) or MHV-A59 spike glycoproteins or chimeric MHV-4/MHV-A59 spike glycoproteins, we have demonstrated the biological functionality of the N-terminus of the spike, encompassing the receptor binding domain (RBD). We...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.593-604.1982
更新日期:1982-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.962-964.1980
更新日期:1980-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
abstract::The ability of the gamma(1)34.5 protein to suppress the PKR response plays a crucial role in herpes simplex virus pathogenesis. In this process, the gamma(1)34.5 protein associates with protein phosphatase 1 to form a large complex that dephosphorylates eIF-2alpha and thereby prevents translation shutoff mediated by P...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7653-7666.2004
更新日期:2004-07-01 00:00:00
abstract::Spatial variation in the epidemiological patterns of successive waves of pandemic influenza virus in humans has been documented throughout the 20th century but never understood at a molecular level. However, the unprecedented intensity of sampling and whole-genome sequencing of the H1N1/09 pandemic virus now makes suc...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
abstract::Japanese encephalitis virus (JEV) is a mosquito-borne pathogenic flavivirus responsible for acute viral encephalitis in humans. The cellular entry of JEV is poorly characterized in terms of molecular requirements and pathways. Here we present a systematic study of the internalization mechanism of JEV in fibroblasts an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01399-12
更新日期:2013-01-01 00:00:00
abstract::Sequencing and reversion analysis of murine hepatitis virus (MHV) temperature-sensitive (ts) viruses has identified putative ts mutations in the replicase nonstructural proteins (nsp's) of these coronaviruses. In this study, reverse transcriptase PCR sequencing of the RNA genome of an isolate of the MHV ts virus Alb t...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.5.3269-3280.2003
更新日期:2003-03-01 00:00:00
abstract::The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00356-15
更新日期:2015-08-01 00:00:00
abstract::Chronic viruses such as herpes simplex virus 1 (HSV-1) evade the hosts' immune system by inducing the exhaustion of antiviral T cells. In the present study, we found that exhausted HSV-specific CD8+ T cells, with elevated expression of programmed death ligand-1 (PD-1) and lymphocyte activation gene-3 (LAG-3) receptors...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00827-19
更新日期:2019-08-28 00:00:00
abstract::The ascomycete Botrytis porri causes clove rot and leaf blight of garlic worldwide. We report here the biological and molecular features of a novel bipartite double-stranded RNA (dsRNA) mycovirus named Botrytis porri RNA virus 1 (BpRV1) from the hypovirulent strain GarlicBc-72 of B. porri. The BpRV1 genome comprises t...
journal_title:Journal of virology
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更新日期:2012-06-01 00:00:00
abstract::The parameters governing the activity of the cloned T4 gene 23, which codes for the major T4 head protein, were analyzed. Suppressor-negative bacteria carrying wild-type T4 gene 23 cloned into plasmid pCR1 or pBR322 were infected with T4 gene 23 amber phage also carrying mutations in the following genes: (i) denA and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.46-59.1981
更新日期:1981-07-01 00:00:00
abstract::Vpu is a 16-kDa membrane-associated phosphoprotein that is expressed from the same, singly spliced message as the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein precursor, gp160. Previous studies suggest that Vpu functions in the late stages of viral replication, possibly in virus egression from the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4190-4194.1993
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.6.1391-1400.1975
更新日期:1975-12-01 00:00:00
abstract::Small ruminants infected with peste des petits ruminants virus exhibit lesions typical of epithelial infection and necrosis. However, the only established host receptor for this virus is the immune cell marker signaling lymphocyte activation molecule (SLAM). We have confirmed that the ovine Nectin-4 protein, when over...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02792-12
更新日期:2013-04-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6) infects >90% of the population and establishes a latent infection with asymptomatic episodes of reactivation. However, HHV-6 reactivation is associated with morbidity and sometimes mortality in immunocompromised patients. To date, control of the virus in healthy virus carriers and the failu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02321-18
更新日期:2019-06-14 00:00:00
abstract::The RNA genome of influenza virus is transcribed and replicated by the viral RNA polymerase complex in the cell nucleus. We have generated green fluorescent protein (GFP)-tagged polymerase subunits to study the assembly of the polymerase complex. Our results show that individually expressed polymerase basic protein 1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9144-9153.2004
更新日期:2004-09-01 00:00:00
abstract::RNA from adenovirus 2-infected KB cells was annealed in liquid with RNA in vast excess to viral heavy (l) and light (r) 32P-labeled DNA strands. Hybridization kinetics were analyzed by computer to estimate the number of viral RNA abundance classes, their relative concentrations, and the fraction of each DNA strand fro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.2.465-477.1976
更新日期:1976-11-01 00:00:00
abstract::Although the herpes simplex virus type 1 (HSV-1) tegument is comprised of a large number of viral and cellular proteins, how and where in the cell these proteins are recruited into the virus structure is poorly understood. We have shown previously that the immediate-early gene product ICP0 is packaged by a mechanism d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00126-10
更新日期:2010-05-01 00:00:00
abstract::The structure of Bacillus subtilis bacteriophage phi25 and phi25 deoxyribonucleic acid (DNA) were studied by electron microscopy. The head of phi25 is a regular polyhedron measuring 75 nm in diameter. The uncontracted tail of phi25 is 130 nm in length and includes a large, complex tail plate. Phage phi25 DNA is double...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.6.1.107-113.1970
更新日期:1970-07-01 00:00:00
abstract::The process of Epstein-Barr virus (EBV)-induced transformation of human B lymphocytes results in a cell line that is a mixture of latently and lytically infected cells, with the lytic cells composing roughly 5% to less than 0.0001% of the overall population. A set of nine normal lymphoblastoid cell lines that span a 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.1.437-444.1990
更新日期:1990-01-01 00:00:00
abstract::A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.32.3.951-957.1979
更新日期:1979-12-01 00:00:00
abstract::The nonstructural protein (NSs) of severe fever with thrombocytopenia syndrome phlebovirus (SFTSV) sequesters TANK-binding kinase 1 (TBK1) into NSs-induced cytoplasmic structures to inhibit the phosphorylation and nuclear translocation of interferon (IFN) regulatory factor 3 (IRF3) and subsequent interferon beta (IFN-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00706-18
更新日期:2018-09-12 00:00:00