Abstract:
:A contiguously linked T-B synthetic viral epitope (110HA120-150HA159,T-B) was investigated for its potency in inducing humoral and cellular immune responses in vivo. The T-cell epitope 110HA120 corresponds to the site 1 hemagglutinin (HA) of the A/PR/8/34 (PR8) influenza virus and is recognized by CD4 T cells in association with I-Ed class II major histocompatibility complex molecules. The 150HA159 represents a major B-cell epitope of the HA protein. T-B dipeptide emulsified in Freund's complete adjuvant was able to induce strong antiviral antibody titers and a high frequency of specific T-cell precursors after a single inoculation in BALB/c mice. In contrast, immunization under identical conditions with equimolar mixtures of T and B peptides did not elicit antibody titers or a cellular immune response. As indicated by the isotypes of antiviral antibodies, the T-B dipeptide preferentially induced a Th1-like immune response. Challenge with T-B dipeptide, but not with T or B peptide alone, stimulated peptide-specific T memory cells in mice previously primed with PR8 virus or with T-B dipeptide. As a consequence, 71 and 57% of these mice, respectively, survived infection with two 100% lethal doses of PR8 virus. Our results suggest that, inasmuch as contiguity between T- and B-cell epitopes provides enough signaling capacity to trigger the mechanisms of T-B-cell cooperation in vivo, a T-B contiguous epitope may well represent a minimal built-in subunit vaccine. Aside from their potential bioavailability, the T-B contiguous epitopes may also represent attractive tools for investigating the molecular mechanisms of T-B-cell cooperation responsible for antiviral protection.
journal_name
J Viroljournal_title
Journal of virologyauthors
Brumeanu TD,Casares S,Bot A,Bot S,Bona CAdoi
10.1128/JVI.71.7.5473-5480.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
5473-80issue
7eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
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pub_type: 杂志文章
doi:10.1128/JVI.64.11.5412-5419.1990
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.6.5.667-676.1970
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3494-3503.1992
更新日期:1992-06-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.37.1.248-255.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-07-01 00:00:00
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journal_title:Journal of virology
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更新日期:2003-05-01 00:00:00
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doi:10.1128/JVI.42.3.847-853.1982
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journal_title:Journal of virology
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更新日期:1986-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.2276-2284.1993
更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00