Abstract:
:We have recovered a virion from defective cDNA of Sendai virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene expression vector derived from a defective viral genome of the Paramyxoviridae. First, functional ribonucleoprotein complexes (RNPs) were recovered from SeV cloned cDNA defective in the F (envelope fusion protein) gene, in the presence of plasmids expressing nucleocapsid protein and viral RNA polymerase. Then the RNPs were transfected to the cells inducibly expressing F protein. Virion-like particles thus obtained had a titer of 0.5 x 10(8) to 1. 0 x 10(8) cell infectious units/ml and contained F-defective RNA genome. This defective vector amplified specifically in an F-expressing packaging cell line in a trypsin-dependent manner but did not spread to F-nonexpressing cells. This vector infected and expressed an enhanced green fluorescent protein reporter gene in various types of animal and human cells, including nondividing cells, with high efficiency. These results suggest that this vector has great potential for use in human gene therapy and vaccine delivery systems.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li HO,Zhu YF,Asakawa M,Kuma H,Hirata T,Ueda Y,Lee YS,Fukumura M,Iida A,Kato A,Nagai Y,Hasegawa Mdoi
10.1128/jvi.74.14.6564-6569.2000subject
Has Abstractpub_date
2000-07-01 00:00:00pages
6564-9issue
14eissn
0022-538Xissn
1098-5514journal_volume
74pub_type
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doi:10.1128/JVI.19.2.490-494.1976
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.698-700.1972
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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