Abstract:
:Rabies continues to present a public health threat in most countries of the world. The most efficient way to prevent and control rabies is to implement vaccination programs for domestic animals. However, traditional inactivated vaccines used in animals are costly and have relatively low efficiency, which impedes their extensive use in developing countries. There is, therefore, an urgent need to develop single-dose and long-lasting rabies vaccines. However, little information is available regarding the mechanisms underlying immunological memory, which can broaden humoral responses following rabies vaccination. In this study, a recombinant rabies virus (RABV) that expressed murine interleukin-7 (IL-7), referred to here as rLBNSE-IL-7, was constructed, and its effectiveness was evaluated in a mouse model. rLBNSE-IL-7 induced higher rates of T follicular helper (Tfh) cells and germinal center (GC) B cells from draining lymph nodes (LNs) than the parent virus rLBNSE. Interestingly, rLBNSE-IL-7 improved the percentages of long-lived memory B cells (Bmem) in the draining LNs and plasma cells (PCs) in the bone marrow (BM) for up to 360 days postimmunization (dpi). As a result of the presence of the long-lived PCs, it also generated prolonged virus-neutralizing antibodies (VNAs), resulting in better protection against a lethal challenge than that seen with rLBNSE. Moreover, consistent with the increased numbers of Bmem and PCs after a boost with rLBNSE, rLBNSE-IL-7-immunized mice promptly produced a more potent secondary anti-RABV neutralizing antibody response than rLBNSE-immunized mice. Overall, our data suggest that overexpressing IL-7 improved the induction of long-lasting primary and secondary antibody responses post-RABV immunization.IMPORTANCE Extending humoral immune responses using adjuvants is an important method to develop long-lasting and efficient vaccines against rabies. However, little information is currently available regarding prolonged immunological memory post-RABV vaccination. In this study, a novel rabies vaccine that expressed murine IL-7 was developed. This vaccine enhanced the numbers of Tfh cells and the GC responses, resulting in upregulated quantities of Bmem and PCs. Moreover, we found that the long-lived PCs that were elicited by the IL-7-expressing recombinant virus (rLBNSE-IL-7) were able to sustain VNA levels much longer than those elicited by the parent rLBNSE virus. Upon reexposure to the pathogen, the longevous Bmem, which maintained higher numbers for up to 360 dpi with rLBNSE-IL-7 compared to rLBNSE, could differentiate into antibody-secreting cells, resulting in rapid and potent secondary production of VNAs. These results suggest that the expression of IL-7 is beneficial for induction of potent and long-lasting humoral immune responses.
journal_name
J Viroljournal_title
Journal of virologyauthors
Li Y,Zhou M,Luo Z,Zhang Y,Cui M,Chen H,Fu ZF,Zhao Ldoi
10.1128/JVI.02324-16subject
Has Abstractpub_date
2017-03-13 00:00:00issue
7eissn
0022-538Xissn
1098-5514pii
JVI.02324-16journal_volume
91pub_type
杂志文章abstract::We report here the complete genomic sequence of the duck Tembusu virus (DTMUV) WJ-1 strain, isolated from Muscovy ducks. This is the first complete genome sequence of DTMUV reported in southern China. Compared with the other strains (TA, GH-2, YY5, and ZJ-407) that were previously found in eastern China, WJ-1 bears a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02361-12
更新日期:2012-12-01 00:00:00
abstract::The mechanism whereby picornaviruses inhibit host protein synthesis while their own synthetic processes proceed unabated has remained elusive. One of our approaches to this problem was to study the ability of cell-free extracts derived from uninfected and mengovirus-infected Ehrlich ascites tumor cells to translate vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.182-194.1976
更新日期:1976-04-01 00:00:00
abstract::Hepatitis C virus (HCV) is an important human pathogen affecting an estimated 3% of the world's population. Recent advances have enabled in vitro propagation of the virus and allow assembly and egress to be investigated for the first time. As a component of the virion, the HCV core protein likely functions primarily i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00793-07
更新日期:2007-10-01 00:00:00
abstract::The mechanisms involved in the emergence of virulent mammalian viruses were investigated in the adult immunodeficient SCID mouse infected by the attenuated prototype strain of the parvovirus Minute Virus of Mice (MVMp). Cloned MVMp intravenously inoculated in mice consistently evolved during weeks of subclinical infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.11280-11290.2005
更新日期:2005-09-01 00:00:00
abstract::A herpes simplex virus type 1 variant [C239(TK-)] harboring a deletion in the thymidine kinase (TK) gene was assessed for capacity to establish latent infections. Outbred Swiss Webster mice were inoculated on both hind footpads, and numbers of neurons expressing latency-associated transcript and amounts of viral DNA i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4976-4978.1989
更新日期:1989-11-01 00:00:00
abstract::In this report, we describe the isolation, molecular genetic mapping, and phenotypic characterization of vaccinia virus mutants resistant to cytosine arabinoside (araC) and phosphonoacetic acid (PAA). At 37 degrees C, 8 microM araC was found to prevent macroscopic plaque formation by wild-type virus and to cause a 10(...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4323-4336.1993
更新日期:1993-07-01 00:00:00
abstract::The roles of intracellular naked (INV), cell-associated enveloped (CEV), and extracellular enveloped (EEV) forms of vaccinia virus in cell-to-cell and longer-range spread were investigated by using two closely related strains of vaccinia virus, WR and IHD-J. We confirmed previous results that WR and IHD-J produced sim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4170-4179.1992
更新日期:1992-07-01 00:00:00
abstract::Bacteriophage T4 gene 32 encodes a DNA unwinding protein required for DNA replication, repair, and recombination. Gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.490-494.1976
更新日期:1976-08-01 00:00:00
abstract::DNA sequence analysis has revealed that the gene coding for the human cytomegalovirus (HCMV) DNA polymerase is present within the long unique region of the virus genome. Identification is based on extensive amino acid homology between the predicted HCMV open reading frame HFLF2 and the DNA polymerase of herpes simplex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.125-133.1987
更新日期:1987-01-01 00:00:00
abstract:UNLABELLED:Modified vaccinia virus Ankara (MVA) serves as a versatile platform in vaccine development. This highly attenuated orthopoxvirus, which cannot replicate in mammalian cells, triggers strong innate immune responses, including cell migration. Previously, we have shown that induction of chemokine (C-C motif) lig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01524-14
更新日期:2014-09-01 00:00:00
abstract::Zika virus (ZIKV) envelope (E) protein is the major target of neutralizing antibodies in infected hosts and thus represents a candidate of interest for vaccine design. However, a major concern in the development of vaccines against ZIKV and the related dengue virus is the induction of cross-reactive poorly neutralizin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01415-20
更新日期:2020-12-09 00:00:00
abstract::Type I interferons (IFNs), as major components of the innate immune system, play a vital role in host resistance to a variety of pathogens. Canonical signaling mediated by type I IFNs activates the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway through binding to the IFN-α/β receptor ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01161-18
更新日期:2018-09-12 00:00:00
abstract::Simian virus 40 (SV40) large T antigen (Tag) is a promiscuous transcriptional transactivator; however, its mechanism of transactivation remains unknown. Recent studies have suggested the possible involvement of protein-protein interactions with TBP, the TATA box-binding protein of TFIID, and TEF-1, an enhancer-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.2.1191-1202.1996
更新日期:1996-02-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. In other picornaviruses, homodimerization of 3A has been shown to be relevant for its biological activity. In this work, FMDV 3A homodimerization was evidenced by an in situ protein fluor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00580-12
更新日期:2012-10-01 00:00:00
abstract::Mouse-adapted dengue type 4 virus (DEN4) strain H241 is highly neurovirulent for mice, whereas its non-mouse-adapted parent is rarely neurovirulent. The genetic basis for the neurovirulence of the mouse-adapted mutant was studied by comparing intratypic chimeric viruses that contained the three structural protein gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.11.6567-6575.1993
更新日期:1993-11-01 00:00:00
abstract::Chromatography of RNA polymerase purified from vaccinia virions and from vaccinia virus-infected HeLa cells resulted in the separation of populations active for early and late transcription. An RNA polymerase population immunodepleted for the vaccinia virus H4 gene peptide could support late transcription. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2602-2604.1995
更新日期:1995-04-01 00:00:00
abstract::Adenovirus type 19 is a major cause of epidemic keratoconjunctivitis, the only ocular adenoviral infection associated with prolonged corneal inflammation. In this study, we investigated the role of phosphoinositide 3-kinase (PI3K) and Akt and their downstream targets in adenovirus infection, and here we report the nov...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.19.12332-12341.2005
更新日期:2005-10-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) mutants impaired in the activities of the structural protein VP16 and the immediate-early (IE) proteins ICP0 and ICP4 establish a quiescent infection in human fibroblasts, with most cells retaining an inactive, repressed viral genome for sustained periods in culture. To date, the qu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01273-08
更新日期:2008-12-01 00:00:00
abstract::Epstein-Barr virus (EBV) is associated with about 6 to 16% of gastric carcinoma cases worldwide. Expression of the EBV microRNAs (miRNAs) was observed in B cells and nasopharyngeal carcinoma cells infected with EBV. However, it is not clear if the EBV miRNAs are expressed in EBV-associated gastric carcinomas (EBVaGCs)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02271-06
更新日期:2007-01-01 00:00:00
abstract::To date, no vaccine that is safe and effective against herpes simplex virus 2 (HSV-2) disease has been licensed. In this study, we evaluated a DNA prime-formalin-inactivated-HSV-2 (FI-HSV2) boost vaccine approach in the guinea pig model of acute and recurrent HSV-2 genital disease. Five groups of guinea pigs were immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02521-10
更新日期:2011-04-01 00:00:00
abstract::Murine gammaherpesvirus 68 (MHV68) is a gammaherpesvirus that was first isolated from murid rodents. MHV68 establishes a latent infection in the spleen and other lymphoid organs. Several gammaherpesviruses, including herpesvirus saimiri, human herpesvirus 8, and MHV68, encode proteins with extensive homology to the D-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.7016-7023.2000
更新日期:2000-08-01 00:00:00
abstract::Echovirus 1 (EV1) is a human pathogen which belongs to the Picornaviridae family of RNA viruses. We have analyzed the early events of infection after EV1 binding to its receptor alpha 2 beta 1 integrin and elucidated the route by which EV1 gains access to the host cell. EV1 binding onto the cell surface and subsequent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.4.1856-1865.2002
更新日期:2002-02-01 00:00:00
abstract::The simian virus 40 (SV40) core origin of replication consists of three functional domains. The sequence 5'-CACTACTTCTGGAATAG-3' with an imperfect inverted repeat (underlined), a palindrome with four 5'-GAGGC-3' pentanucleotide repeats, and a 17-base-pair A + T-rich segment. We have been able to assign primary functio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.509-518.1990
更新日期:1990-02-01 00:00:00
abstract::We have previously identified 11 epitopes located in two topologically nonoverlapping antigenic sites (A and B) and a third bridging site (C) on the human type 3 parainfluenza virus (PIV3) hemagglutinin-neuraminidase (HN) glycoprotein by using monoclonal antibodies (MAbs) which inhibit hemagglutination and virus infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.90-96.1986
更新日期:1986-10-01 00:00:00
abstract::The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4534-4539.1990
更新日期:1990-09-01 00:00:00
abstract::Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the doma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11301-11311.2002
更新日期:2002-11-01 00:00:00
abstract::Partial deletion of the second hypervariable region from the envelope of the primary-like SF162 virus increases the exposure of certain neutralization epitopes and renders the virus, SF162DeltaV2, highly susceptible to neutralization by clade B and non-clade B human immunodeficiency virus (HIV-positive) sera (L. Stama...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.12.5526-5540.2001
更新日期:2001-06-01 00:00:00
abstract::Cytotoxic T-lymphocyte (CTL) responses are crucial for the control of immunodeficiency virus replication. Possible involvement of a dominant single epitope-specific CTL in control of viral replication has recently been indicated in preclinical AIDS vaccine trials, but it has remained unclear if multiple epitope-specif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1949-1958.2006
更新日期:2006-02-01 00:00:00
abstract::A herpes simplex virus type 1 (HSV-1) strain, B3, was found to have a short duplication on the left junction between the unique sequence (US) and the inverted repeat sequence (RS) in the S component of the genome DNA. A short region of RS contiguous to the left US-RS junction was duplicated in B3. Based on the nucleot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1877-1883.1989
更新日期:1989-05-01 00:00:00
abstract::S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.1.221-224.1987
更新日期:1987-01-01 00:00:00