Abstract:
:Herpes simplex virus type 1 (HSV-1) mutants impaired in the activities of the structural protein VP16 and the immediate-early (IE) proteins ICP0 and ICP4 establish a quiescent infection in human fibroblasts, with most cells retaining an inactive, repressed viral genome for sustained periods in culture. To date, the quiescent state has been considered stable, since it has been reversed only by provision of herpesviral proteins, such as ICP0, not by alteration of the cell physiological state. We report that the interaction of HSV-1 with human fibroblasts can be altered significantly by transient treatment of cultures with sodium arsenite, an inducer of heat shock and oxidative stress, or gramicidin D, a toxin that selectively permeabilizes cell membranes, prior to infection. These regimens stimulated gene expression from IE-deficient HSV-1 mutants in a promoter sequence-independent manner and also overcame the replication defect of ICP0-null mutants. Reactivation of gene expression from quiescent HSV-1 genomes and the resumption of virus replication were observed following addition of arsenite or gramicidin D to cultures. Both agents induced reorganization of nuclear domain 10 structures, the sites of quiescent genomes, but appeared to do so through different mechanisms. The results demonstrate that the physiological state of the cell is important in determining the outcome of infection with IE-deficient HSV-1 and show novel methods for reactivating quiescent HSV-1 in fibroblasts with a high efficiency.
journal_name
J Viroljournal_title
Journal of virologyauthors
Preston CM,Nicholl MJdoi
10.1128/JVI.01273-08subject
Has Abstractpub_date
2008-12-01 00:00:00pages
11775-83issue
23eissn
0022-538Xissn
1098-5514pii
JVI.01273-08journal_volume
82pub_type
杂志文章abstract::The small envelope protein (E) plays a role of central importance in the assembly of coronaviruses. This was initially established by studies demonstrating that cellular expression of only E protein and the membrane protein (M) was necessary and sufficient for the generation and release of virus-like particles. To inv...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01577-06
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journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.509-518.1990
更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1969-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.3.950-958.2002
更新日期:2002-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7106-7112.1995
更新日期:1995-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.6.3534-3543.1993
更新日期:1993-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.8.4130-4136.1991
更新日期:1991-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.3.1059-1068.1989
更新日期:1989-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00362-14
更新日期:2014-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1803-1807.1989
更新日期:1989-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.472-485.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2033-2040.1990
更新日期:1990-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02743-05
更新日期:2006-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4508-4514.1998
更新日期:1998-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.400-410.1994
更新日期:1994-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
更新日期:1996-06-01 00:00:00
abstract:UNLABELLED:The western honeybee (Apis mellifera) is the most important commercial insect pollinator. However, bees are under pressure from habitat loss, environmental stress, and pathogens, including viruses that can cause lethal epidemics. Slow bee paralysis virus (SBPV) belongs to the Iflaviridae family of nonenvelop...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-07-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.15.1.137-144.1975
更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7383-7393.1993
更新日期:1993-12-01 00:00:00
abstract::Langat virus (LGT), the naturally attenuated member of the tick-borne encephalitis virus (TBEV) complex, was tested extensively in clinical trials as a live TBEV vaccine and was found to induce a protective, durable immune response; however, it retained a low residual neuroinvasiveness in mice and humans. In order to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.3.1427-1439.2006
更新日期:2006-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01708-19
更新日期:2020-03-17 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1682-1688.1999
更新日期:1999-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.10.3574-3580.1988
更新日期:1988-10-01 00:00:00