Abstract:
UNLABELLED:Modified vaccinia virus Ankara (MVA) serves as a versatile platform in vaccine development. This highly attenuated orthopoxvirus, which cannot replicate in mammalian cells, triggers strong innate immune responses, including cell migration. Previously, we have shown that induction of chemokine (C-C motif) ligand 2 (CCL2) by MVA is necessary for the recruitment of monocytes and T cells, but not neutrophils, to the lung. Here, we identified neutrophil-attracting chemokines produced by MVA-infected primary murine lung fibroblasts and murine bone marrow-derived macrophages. We demonstrate that MVA, but not vaccinia virus (VACV) strain WR, induces chemokine expression, which is independent of Toll-like receptor 2 (TLR2) signaling. Additionally, we show that both chemokine (C-C motif) receptor 1 (CCR1) and chemokine (C-X-C motif) receptor 2 (CXCR2) are involved in MVA-induced neutrophil chemotaxis in vitro. Finally, intranasal infection of Ccr1(-/-) mice with MVA, as well as application of the CCR1 antagonist J-113863, revealed a role for CCR1 in leukocyte recruitment, including neutrophils, into the lung. IMPORTANCE:Rapid attraction of leukocytes to the site of inoculation is unique to MVA in comparison to other VACV strains. The findings here extend current knowledge about the regulation of MVA-induced leukocyte migration, particularly regarding neutrophils, which could potentially be exploited to improve other VACV strains currently in development as oncolytic viruses and viral vectors. Additionally, the data presented here indicate that the inflammatory response may vary depending on the cell type infected by MVA, highlighting the importance of the site of vaccine application. Moreover, the rapid recruitment of neutrophils and other leukocytes can directly contribute to the induction of adaptive immune responses elicited by MVA inoculation. Thus, a better understanding of leukocyte migration upon MVA infection is particularly relevant for further development and use of MVA-based vaccines and vectors.
journal_name
J Viroljournal_title
Journal of virologyauthors
Price PJ,Luckow B,Torres-Domínguez LE,Brandmüller C,Zorn J,Kirschning CJ,Sutter G,Lehmann MHdoi
10.1128/JVI.01524-14subject
Has Abstractpub_date
2014-09-01 00:00:00pages
10840-50issue
18eissn
0022-538Xissn
1098-5514pii
JVI.01524-14journal_volume
88pub_type
杂志文章abstract::Infectious cDNA corresponding to the entire genome of the attenuated Sabin strain of type 1 poliovirus has been inserted into EcoRI site of bacterial plasmid pBR325. Two consecutive PstI fragments (nucleotide positions 1814 to 3421) of the infectious cDNA of the Sabin 1 strain were replaced by the corresponding DNA fr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.53.3.786-792.1985
更新日期:1985-03-01 00:00:00
abstract::We have investigated the mechanism of duck hepatitis B virus (DHBV) entry into susceptible primary duck hepatocytes (PDHs), using mutants of carboxypeptidase D (gp180), a transmembrane protein shown to act as the primary cellular receptor for avian hepatitis B virus uptake. The variant proteins were abundantly produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2203-2209.2000
更新日期:2000-03-01 00:00:00
abstract::PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.1.195-204.1976
更新日期:1976-04-01 00:00:00
abstract::Signal peptidase cleavage at the C-prM junction in the flavivirus structural polyprotein is inefficient in the absence of the cytoplasmic viral protease, which catalyzes cleavage at the COOH terminus of the C protein. The signal peptidase cleavage occurs efficiently in circumstances where the C protein is deleted or i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2141-2149.1998
更新日期:1998-03-01 00:00:00
abstract::When residues 17 and 18 in nef of simian immunodeficiency virus strain SIVmac239 were changed from RQ to YE, the resultant virus was able to replicate in peripheral blood mononuclear cell cultures without prior lymphocyte activation and without the addition of exogenous interleukin-2, caused extensive lymphocyte activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.4157-4161.1996
更新日期:1996-06-01 00:00:00
abstract::The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for transcriptional regulation of viral genes and partitioning of viral genomes. Bovine papillomavirus type 1 (BPV-1) E2 binds cellular chromatin in complex with Brd4 in both mitotic and interphase cells. To identify specific sites o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02275-08
更新日期:2009-03-01 00:00:00
abstract::Nuclear domains called ND10 (nuclear domain 10) are discrete nuclear protein aggregations characterized by a set of interferon-upregulated proteins including Sp100 and PML, where papova-, adeno-, and herpesviruses begin their transcription and DNA replication. Both the alpha- and betaherpesvirus subfamilies disrupt ND...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11800-11810.2000
更新日期:2000-12-01 00:00:00
abstract::Strategies were developed to insert the neo resistance marker into the junction between L DNA and the right terminal repetitive H DNA of herpesvirus saimiri. Recombinant viruses were selectable in permissive epithelioid cultures. The human T-cell line Jurkat could be infected persistently with the Neor virus; the cell...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.4.1818-1821.1989
更新日期:1989-04-01 00:00:00
abstract::Foamy viruses (FVs) are the least known retroviruses commonly found in primates, cats, horses, and cattle. Although FVs are considered apathogenic, simian and feline FVs have been shown to be associated with some transient health abnormalities in animal models. Currently, data regarding the course of infection with bo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-12
更新日期:2013-03-01 00:00:00
abstract::To determine whether the tripartite leader is required for efficient translation in adenovirus-infected cells at late times of infection, we constructed recombinant adenoviruses containing the influenza virus nucleocapsid protein (NP) gene expressed under the control of the adenovirus major late promoter (MLP). We cho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1606-1616.1988
更新日期:1988-05-01 00:00:00
abstract::Vaccinia virus DNA fragments that have been denatured by alkali and then neutralized contain a fraction that rapidly reforms duplex structures. The fraction is enriched by fractionating on hydroxyapatite columns and serves as as substrate for digestion by two restriction endonucleases isolated from Hemophilus parainfl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.227-238.1976
更新日期:1975-01-01 00:00:00
abstract::Infection of normal rat fibroblasts (FR 3T3) with the early tsa mutant of polyoma virus may lead to either the A or the N phenotype, tsa-A transformants, originally derived by agar selection, are not temperature dependent for maintenance of the transformed phenotype, whereas tsa-N transormants revert at high temperatu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.2.421-426.1978
更新日期:1978-11-01 00:00:00
abstract::The interaction of the human immunodeficiency virus (HIV) Gag protein with the plasma membrane of a cell is a critical event in the assembly of HIV particles. The matrix protein region (MA) of HIV type 1 (HIV-1) Pr55Gag has previously been demonstrated to confer membrane-binding properties on the precursor polyprotein...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6582-6592.1997
更新日期:1997-09-01 00:00:00
abstract:UNLABELLED:During hepatitis C virus (HCV) infection, broadly neutralizing antibody (bNAb) responses targeting E1E2 envelope glycoproteins are generated in many individuals. It is unclear if these antibodies play a protective or a pathogenic role during chronic infection. In this study, we investigated whether bNAb resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02669-15
更新日期:2016-04-14 00:00:00
abstract:UNLABELLED:Children with acute respiratory syncytial virus (RSV) infection often develop sequelae of persistent airway inflammation and wheezing. Pulmonary C fibers (PCFs) are involved in the generation of airway inflammation and resistance; however, their role in persistent airway diseases after RSV is unexplored. Her...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02534-15
更新日期:2015-12-16 00:00:00
abstract::In-frame stop codons were introduced into the coding region of human immunodeficiency virus type 1 (HIV-1) transmembrane protein (gp41). Truncation of 147 amino acids from the carboxyl terminus of gp41 (TM709) significantly decreased the stability and cell surface expression of the viral Env proteins, while truncation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.1.213-221.1993
更新日期:1993-01-01 00:00:00
abstract::Retrovirus particles each contain two copies of the viral genome in the form of a noncovalently linked RNA dimer. Earlier studies have mapped a cis-acting region near the 5' end of the human immunodeficiency virus type 1 (HIV-1) genome, termed the psi locus, which appears essential for initiation of genomic dimerizati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3407-3414.1997
更新日期:1997-05-01 00:00:00
abstract:UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-16
更新日期:2016-08-12 00:00:00
abstract::The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We used electron cryomicroscopy and antibody labeling to show that the 8-kDa SCP of human cytomegalovirus is attached only to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.1327-1332.2005
更新日期:2005-01-01 00:00:00
abstract::A number of naturally occurring hepatitis B virus mutants that cannot synthesize the virus precore protein have been identified. Such mutants have been associated with more severe forms of hepatitis, including fulminant hepatitis. The most common mutation observed is a substitution of G to A in the distal precore gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.9.5682-5684.1992
更新日期:1992-09-01 00:00:00
abstract::Avian leukosis virus subgroup J (ALV-J), an exogenous avian retrovirus, is thought to have evolved by recombination with the highly identical env gene of the endogenous avian retrovirus EAV-HP. Embryonic expression of EAV-HP env has been suggested to be associated with the induction of immunological tolerance, a featu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11935-11938.2001
更新日期:2001-12-01 00:00:00
abstract::Both CD4(+) and CD8(+) T cells are required for clearance of the murine coronavirus mouse hepatitis virus (MHV) during acute infection. We investigated the effects of an epitope-specific CD8(+) T-cell response on acute infection of MHV, strain A59, in the murine CNS. Mice with CD8(+) T cells specific for gp33-41 (an H...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.3.1150-1159.2004
更新日期:2004-02-01 00:00:00
abstract::cis-Acting elements in the viral genome RNA (vRNA) are essential for the translation, replication, and/or encapsidation of RNA viruses. In this study, a novel conserved cis-acting element was identified in the capsid-coding region of mosquito-borne flavivirus. The downstream of 5' cyclization sequence (5'CS) pseudokno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00243-13
更新日期:2013-06-01 00:00:00
abstract::Infection of domestic rabbits with cottontail rabbit papillomavirus (CRPV) causes local papillomas which progress to carcinomas in more than 80% of cases. This animal model system therefore allows the identification of molecular mechanisms required for the induction and progression of epithelial tumors. The viral E2 p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.22.11209-11215.2002
更新日期:2002-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) possesses low pathogenic potential in an immunocompetent host. In the immunosuppressed host, however, a wide spectrum of infection outcomes, ranging from asymptomatic to life threatening, can follow either primary or nonprimary infection. The variability in the manifestations of HCMV infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7661-7671.2002
更新日期:2002-08-01 00:00:00
abstract::Influenza A virus (IAV) is a highly transmissible respiratory pathogen and a major cause of morbidity and mortality around the world. Nucleoprotein (NP) is an abundant IAV protein essential for multiple steps of the viral life cycle. Our recent proteomic study of the IAV-host interaction network found that TRIM41 (tri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00905-18
更新日期:2018-07-31 00:00:00
abstract::Rhesus rhadinovirus (RRV) is a gammaherpesvirus closely related to Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic virus linked to the development of Kaposi's sarcoma and several other lymphoproliferative diseases, including primary effusion lymphoma and multicentric Castleman's disease. RRV naturally inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01429-10
更新日期:2010-11-01 00:00:00
abstract::The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), the type species of alphabaculoviruses, is an enveloped DNA virus that infects lepidopteran insects and is commonly known as a vector for protein expression and cell transduction. AcMNPV belongs to a diverse group of viral and bacterial pat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00111-18
更新日期:2018-05-14 00:00:00
abstract::To analyze intrauterine transmission, MT-2 cells, a human T-cell line producing human T-cell leukemia virus type I (HTLV-I), were injected into eight pregnant F344 rats, and cesarean section was performed at day 23 of pregnancy. HTLV-I provirus was detected by PCR in the liver and spleen taken from one of the eight fe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1302-1305.1995
更新日期:1995-02-01 00:00:00
abstract::An important site for bovine herpesvirus 1 (BoHV-1) latency is sensory neurons within trigeminal ganglia (TG). The synthetic corticosteroid dexamethasone consistently induces BoHV-1 reactivation from latency. Expression of four Krüppel-like transcription factors (KLF), i.e., KLF4, KLF6, PLZF (promyelocytic leukemia zi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01670-19
更新日期:2020-01-31 00:00:00