Abstract:
:PM2 DNA was prepared with different superhelical densities (sigma) in order to examine the relationship betweenn supercoiling and the occurrence of a region(s) of unpaired bases in this DNA. A previous study showed that CH3HgOH reacts with native superhelical PM2 DNA more rapidly than the nicked form II. This evaluation of binding, monitored through the change of sedimentation velocity, was repeated on PM2 DNA I with different superhelical densities. Early binding is detected by an increase in sedimentation velocity and occurs with molecules with sigma' values betwee -0.025 and -0.037. The conversion of form I to form II with the single-strand-specific endonuclease from Neurospora crassa also occurs above a sigma value of -0.025. This data strongly supports the view that supercoiling produces interrupted secondary structure. The question whether the interrupted regions remain single stranded in character or form small intrastrand hairpin regions is considered by examining which model best fits the CH3HgOH- induced sedimentation velocity changes and the standard sedimentation velocity versus the superhelical density curve for the in vitro made DNAs. The hairpin model offers the most satisfactory explanations for all the results of this and previous studies.
journal_name
J Viroljournal_title
Journal of virologyauthors
Woodworth-Gutai M,Lebowitz Jdoi
10.1128/JVI.18.1.195-204.1976subject
Has Abstractpub_date
1976-04-01 00:00:00pages
195-204issue
1eissn
0022-538Xissn
1098-5514journal_volume
18pub_type
杂志文章abstract::The papillomavirus (PV) E2 protein is a critical regulator of viral transcription and genome replication. We previously reported that tyrosine (Y) 138 of HPV-31 E2 is phosphorylated by the fibroblast growth factor receptor 3 (FGFR3) kinase. In this study, we generated quasiviruses containing G418-selectable HPV-31 gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00488-20
更新日期:2020-07-01 00:00:00
abstract::We have investigated the mechanism of duck hepatitis B virus (DHBV) entry into susceptible primary duck hepatocytes (PDHs), using mutants of carboxypeptidase D (gp180), a transmembrane protein shown to act as the primary cellular receptor for avian hepatitis B virus uptake. The variant proteins were abundantly produce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2203-2209.2000
更新日期:2000-03-01 00:00:00
abstract::Human immunodeficiency virus type 1 contains a transmembrane glycoprotein with an unusually long cytoplasmic domain. To determine the role of this domain in virus replication, a series of single nucleotide changes that result in the insertion of premature termination codons throughout the cytoplasmic domain has been c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6616-6625.1992
更新日期:1992-11-01 00:00:00
abstract::AV3 cells (continuous human amnion) infected with the Onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to AV3 cell monolayers. An apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.10.6.1179-1183.1972
更新日期:1972-12-01 00:00:00
abstract::Extracts from poliovirus-infected HeLa cells are unable to translate vesicular stomatitis virus or cellular mRNAs in vitro, probably reflecting the poliovirus-induced inhibition of host cell protein synthesis which occurs in vivo. Crude initiation factors from uninfected HeLa cells are able to restore translation of v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.1.200-207.1982
更新日期:1982-04-01 00:00:00
abstract::White spot syndrome virus (WSSV) is a crustacean-infecting, double-stranded DNA virus and is the most serious viral pathogen in the global shrimp industry. WSSV is the sole recognized member of the family Nimaviridae, and the lack of genomic data on other nimaviruses has obscured the evolutionary history of WSSV. Here...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01144-18
更新日期:2019-01-17 00:00:00
abstract::Nectin-1 and nectin-2 are related molecules that can function with different specificities as entry receptors for mammalian alphaherpesviruses through interaction with viral glycoprotein D (gD). The normal function of members of the nectin family is to mediate cell-cell adhesion through homotypic and heterotypic necti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12940-12950.2002
更新日期:2002-12-01 00:00:00
abstract::Murine gammaherpesvirus 68 (gammaHV68; also known as MHV-68) can establish a latent infection in both inbred and outbred strains of mice and, as such, provides a tractable small-animal model to address mechanisms and cell types involved in the establishment and maintenance of chronic gammaherpesvirus infection. Latenc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8310-8321.2003
更新日期:2003-08-01 00:00:00
abstract:UNLABELLED:Hepatitis B, which caused by hepatitis B virus (HBV) infection, remains a major health threat worldwide. Hepatic injury and regeneration from chronic inflammation are the main driving factors of liver fibrosis and cirrhosis in chronic hepatitis B. Proinflammatory tumor necrosis factor alpha (TNF-α) has been ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03106-14
更新日期:2015-02-01 00:00:00
abstract::Monoclonal antibody-resistant mutants have been widely used to estimate virus mutation frequencies. We demonstrate that standard virion neutralization inevitably underestimates monoclonal antibody-resistant mutant genome frequencies of vesicular stomatitis virus, due to phenotypic masking-mixing when wild-type (wt) vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.12.5030-5036.1989
更新日期:1989-12-01 00:00:00
abstract::The coding capacity of human cytomegalovirus (HCMV) for glycoproteins by far exceeds that of other herpesviruses. Few of these proteins have been characterized so far. We have investigated the gene product of reading frame UL132. The putative protein product of UL132 is a glycoprotein with a theoretical mass of 29.8 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::We report the identification of a functional nuclear localization signal (NLS) in the human cytomegalovirus (HCMV) large tegument protein pUL48 that is required for nuclear localization in transfected cells and is essential for viral growth. The NLS was mapped to pUL48 amino acid residues 284 to 302. This sequence con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03558-12
更新日期:2013-05-01 00:00:00
abstract::Previous studies have suggested that coxsackievirus B (CVB) activates CD8(+) T cells in vivo, but the extent of this activation and the antigen specificity of the CD8(+) T cells remain uncertain. Furthermore, CVB-induced CD4(+) T-cell responses have not been carefully investigated. Herein, we evaluate CD8(+) and CD4(+...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02639-07
更新日期:2008-05-01 00:00:00
abstract::This report describes the results of experiments to determine whether chimeras between a retrovirus and portions of Ty3 are active in vivo. A chimera between Ty3 and a Neo(r)-marked Moloney murine leukemia virus (M-MuLV) was constructed. The C-terminal domain of M-MuLV integrase (IN) was replaced with the C-terminal d...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4297-4307.1998
更新日期:1998-05-01 00:00:00
abstract::Transcription of herpesviral late genes is stimulated after the onset of viral DNA replication but otherwise restricted. Late gene expression in gammaherpesviruses requires the coordination of six early viral proteins, termed viral transactivation factors (vTFs). Here, we mapped the organization of this protein comple...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02157-15
更新日期:2015-10-14 00:00:00
abstract::Suppression of interferon signaling is of paramount importance to a virus. Interferon signaling significantly reduces or halts the ability of a virus to replicate; therefore, viruses have evolved sophisticated mechanisms that suppress activation of the interferon pathway or responsiveness of the infected cell to inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02484-16
更新日期:2017-03-29 00:00:00
abstract::The fusogenic properties of the uncleaved spike (S) protein of murine coronavirus JHMV variant cl-2 were studied by expressing the S protein with a deleted putative cleavage site. The amino acid sequence of the putative cleavage site, Arg-Arg-Ala-Arg-Arg, was replaced by Arg-Thr-Ala-Leu-Glu by in vitro mutagenesis of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1195-1202.1993
更新日期:1993-03-01 00:00:00
abstract::Hemagglutination (HA) by the mammalian reoviruses is mediated by interactions between the viral sigma 1 protein and sialoglycoproteins on the erythrocyte surface. Three serotype 3 (T3) reovirus strains were identified that do not agglutinate either bovine or type O human erythrocytes (HA negative): T3 clone 43 (T3C43)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.5173-5176.1990
更新日期:1990-10-01 00:00:00
abstract::Ebola virus (EBOV) infections result in aggressive hemorrhagic fever in humans, with fatality rates reaching 90% and with no licensed specific therapeutics to treat ill patients. Advances over the past 5 years have firmly established monoclonal antibody (MAb)-based products as the most promising therapeutics for treat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01548-18
更新日期:2019-02-19 00:00:00
abstract::The mechanisms of cell death in CD4+ T cells mediated by human herpesvirus 6 (HHV-6) were investigated. The frequency of cell death in the human CD4+ T-cell line JJHAN, which had been inoculated with HHV-6 variant A or B, appeared to be augmented by tumor necrosis factor alpha (TNF-alpha). Agarose gel electrophoresis ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3751-3759.1997
更新日期:1997-05-01 00:00:00
abstract::Mammalian cells infected with human adenoviruses (Ads) undergo an apoptotic response as a result of expression of the viral E1A proteins, and this process is suppressed by the viral E1B-19K protein. The intermediary steps in the Ad-induced apoptosis pathway are not fully resolved. The apical step in the canonical mamm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00808-07
更新日期:2007-10-01 00:00:00
abstract::By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [(3)H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have studied the fate of pulse-labeled viral RNA from cells at 45 h postinfection under these conditions. During a 5-min period of la...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.28.2.590-599.1978
更新日期:1978-11-01 00:00:00
abstract::In this study, we show that human cytomegalovirus DNA synthesis is inhibited in infected confluent human embryonic lung cells treated with the DNA-intercalative topoisomerase II inhibitor 4-9'-(acridinylamino)methanesulfon-m-anisidide (m-AMSA). Similar inhibitory effects were observed with VM-26, a nonintercalative to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.12.4797-4800.1988
更新日期:1988-12-01 00:00:00
abstract::Examination of [3H]mannose-labeled glycopeptides from Prague C Rous sarcoma virus gp85 with gel filtration and sequential glycosidase digestions demonstrated the presence of hybrid-type asparaginyl-oligosaccharides. The major hybrid species had an oligomannosyl core (Man5GlcNAc2-ASN) characteristic of neutral structur...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.2.646-650.1981
更新日期:1981-08-01 00:00:00
abstract::Although monkey B virus (herpesvirus simiae; BV) is common in all macaque species, fatal human infections appear to be associated with exposure to rhesus macaques (Macaca mulatta), suggesting that BV isolates from rhesus monkeys may be more lethal to nonmacaques than are BV strains indigenous to other macaque species....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.9224-9232.1998
更新日期:1998-11-01 00:00:00
abstract::Treatment of human immunodeficiency virus type 1 (HIV-1)-infected CEM cell cultures with escalating concentrations of the quinoxaline S-2720 resulted in an ordered appearance of single and multiple mutant virus strains that gradually became resistant to the quinoxaline and other nonnucleoside reverse transcriptase (RT...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8195-8203.1997
更新日期:1997-11-01 00:00:00
abstract::MxA is an abundant and ubiquitous cytoplasmic protein induced by alpha/beta interferon in human cells. Upon full induction, it can constitute 0.5 to 1% of cytosolic proteins. MxA can bind elements of the cytoskeleton, such as actin and tubulins, and several larger cellular proteins. However, these protein-protein inte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.8.4705-4709.1992
更新日期:1992-08-01 00:00:00
abstract::Intracellular mature vaccinia virions are wrapped by cisternae, derived from virus-modified trans-Golgi or endosomal membranes, and then transported via microtubules to the cell periphery. Two viral proteins, encoded by the F13L and B5R open reading frames, are essential for the membrane-wrapping step. Previous transf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7777-7789.2002
更新日期:2002-08-01 00:00:00
abstract::Enviroxime is an antienterovirus compound that targets viral protein 3A and/or 3AB and suppresses a step in enterovirus replication by unknown mechanism. To date, four antienterovirus compounds, i.e., GW5074, Flt3 inhibitor II, TTP-8307, and AN-12-H5, are known to have similar mutations in the 3A protein-encoding regi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02249-10
更新日期:2011-03-01 00:00:00
abstract::Natural Killer (NK) cells are important in the immune response to a number of viruses; however, the mechanisms used by NK cells to discriminate between healthy and virus-infected cells are only beginning to be understood. Infection with vaccinia virus provokes a marked increase in the susceptibility of target cells to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.5.2225-2233.2006
更新日期:2006-03-01 00:00:00