Abstract：
:By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [(3)H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have studied the fate of pulse-labeled viral RNA from cells at 45 h postinfection under these conditions. During a 5-min period of labeling, both the messenger and nonmessenger regions of the late strand were transcribed. After various chase periods, nuclear viral species which sediment at 19, 17.5, and 16S were observed. Nuclear viral RNA decays in a multiphasic manner. Of the material present at the beginning of the chase period, 50% was degraded rapidly with a half-life of 8 min (initial processing). This rapidly degraded material was that fraction of the late strand which did not give rise to stable late mRNA species. Forty percent was transported to the cytoplasm, and 10% remained in the nucleus as material which sedimented in the 2 to 4S region. These 2 to 4S viral RNAs had a half-life of 3 h, and hybridization studies suggest that they are in part coded for by the late-strand nonmessenger region and are derived from the initial nuclear processing step. Another part is coded for by the late-strand messenger region and may be generated by some subsequent nuclear cleavages of 19S RNA into 17.5 and 16S RNAs. Transport of nuclear viral RNA into the cytoplasm was detected after a 5-min pulse and a 7-min chase. The maximum amount of labeled viral RNA was accumulated in the cytoplasm after a 30-min to 1-h chase. At least two viral cytoplasmic species were observed. Kinetic data suggest that 19S RNA is transported directly from the nucleus. Whether cytoplasmic 16S is formed by cleavage of 19S RNA in the cytoplasm is not clear. The half-lives of cytoplasmic 19 and 16S RNAs can be approximated as 2 and 5 h, respectively.
journal_name
J Viroljournal_title
Journal of virologyauthors
Chiu NH,Radonovich MF,Thoren MM,Salzman NPdoi
10.1128/JVI.28.2.590-599.1978subject
Has Abstractpub_date
1978-11-01 00:00:00pages
590-9issue
2eissn
0022-538Xissn
1098-5514journal_volume
28pub_type
杂志文章abstract：:Differential effect of various metabolic inhibitors on the development of hemadsorption activity and virus formation in cells infected with Newcastle disease virus (NDV) was investigated. It was found that, in BHK-21 cells infected with NDV, cycloheximide did not prevent the development of hemadsorption activity, wher...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.11.4.479-486.1973
更新日期：1973-04-01 00:00:00
abstract：:Norovirus (NoV) infections are a significant health burden to society, yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02138-16
更新日期：2017-01-18 00:00:00
abstract：:A molecular clone of mouse-neuroadapted yellow fever 17D virus (SPYF-MN) was used to identify critical determinants of viral neuroinvasiveness in a SCID mouse model. Virus derived from this clone differs from nonneuroinvasive YF5.2iv virus at 29 nucleotide positions, encoding 13 predicted amino acid substitutions and ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.77.22.12232-12242.2003
更新日期：2003-11-01 00:00:00
abstract：:The lymphocytic choriomeningitis virus (LCMV) genome consists of a large RNA segment and a small RNA segment. The three major structural proteins of this virus are an internal nucleoprotein and two surface glycoproteins. Intertypic reassortants between the Armstrong and WE strains of LCMV were made to map proteins enc...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.53.3.966-968.1985
更新日期：1985-03-01 00:00:00
abstract：:The mammalian tripartite motif protein, TRIM5α, recognizes retroviral capsids entering the cytoplasm and blocks virus infection. Depending on the particular TRIM5α protein and retrovirus, complete disruption of the TRIM5α RING domain decreases virus-restricting activity to various degrees. TRIM5α exhibits RING domain-...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00341-11
更新日期：2011-08-01 00:00:00
abstract：:Natural evolution in primate lentiviral reverse transcriptase (RT) appears to have been constrained by the necessity to maintain function within an asymmetric protein composed of two identical primary amino acid sequences (66 kDa), of which one is cleaved (51 kDa). In this study, a detailed phylogenetic analysis now s...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.00991-10
更新日期：2010-10-01 00:00:00
abstract：:The Moloney murine leukemia virus (MuLV) is a highly leukemogenic virus. To map the leukemogenic potential of Moloney MuLV, we constructed chimeric viral DNA genomes in vitro between parental cloned infectious viral DNA from Moloney and amphotropic 4070-A MuLVs. Infectious chimeric MuLVs were recovered by microinjecti...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.52.2.448-456.1984
更新日期：1984-11-01 00:00:00
abstract：:The positive-strand RNA bromoviruses encode two nonstructural proteins, 1a and 2a, involved in RNA-dependent RNA replication. These proteins have extensive sequence similarities with methyltransferase, helicase, and polymerase proteins of other plant and animal viruses. 1a and 2a can also form a complex in vitro. To e...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.67.12.7181-7189.1993
更新日期：1993-12-01 00:00:00
abstract：:AV3 cells (continuous human amnion) infected with the Onderstepoort strain of canine distemper virus produced cell fusion within 2 to 5 hr when added to AV3 cell monolayers. An apparent requirement for intact, infected cells was demonstrated by showing that (i) frozen-and-thawed infected cells failed to induce fusion,...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.10.6.1179-1183.1972
更新日期：1972-12-01 00:00:00
abstract：:The papillomavirus capsid mediates binding to the cell surface and passage of the virion to the perinuclear region during infection. To better understand how the virus traffics across the cell, we sought to identify cellular proteins that bind to the minor capsid protein L2. We have identified syntaxin 18 as a protein...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.79.11.6723-6731.2005
更新日期：2005-06-01 00:00:00
abstract：:The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.15.3.534-539.1975
更新日期：1975-03-01 00:00:00
abstract：:The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subseq...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.16.6.1608-1614.1975
更新日期：1975-12-01 00:00:00
abstract：:The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.69.12.8151-8154.1995
更新日期：1995-12-01 00:00:00
abstract：:All presently available replication-competent proviral clones of human immunodeficiency virus type 1 (HIV-1) are derived from cell culture-amplified virus. Since tissue culture is highly selective for viral strains with an in vitro growth advantage, such clones may not be representative of the biologically relevant vi...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.65.8.3973-3985.1991
更新日期：1991-08-01 00:00:00
abstract：:EBNA2 transcriptional activation and regulated EBNALP coactivation are critical for Epstein-Barr virus-infected primary B-lymphocyte growth transformation. EBNALP coactivation requires the EBNA2 acidic activation domain (E2AD); EBNALP can bind to E2AD. EBNALP has now been found to bind less well to EBNA2 amino acids 1...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.78.20.11439-11442.2004
更新日期：2004-10-01 00:00:00
abstract：:The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5. One way in which gPr80 helps MLVs to escape host innate im...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01530-17
更新日期：2018-02-26 00:00:00
abstract：:Recent studies using radiolabeled rotavirus lysates have demonstrated a 35-kilodalton viral protein that binds specifically to the surface of MA104 cells (N. Fukuhara, O. Yoshie, S. Kitakoa, and T. Konno, J. Virol. 62:2209-2218, 1988; M. Sabara, J. Gilchrist, G.R. Hudson, and L.A. Babiuk, J. Virol. 53:58-66, 1985). Th...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.64.1.322-330.1990
更新日期：1990-01-01 00:00:00
abstract：:We have previously shown that expression of the adenovirus E1A 12S or 13S products in NIH 3T3 fibroblasts induces susceptibility to the cytotoxic actions of tumor necrosis factor alpha (TNF alpha). A large number of studies have mapped the multiple biological functions of the 12S and 13S products to three highly conse...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.64.9.4115-4122.1990
更新日期：1990-09-01 00:00:00
abstract：:Jurkat T-cell clones, stably expressing the human immunodeficiency virus type 1 (HIV-1) Vpr protein, exhibited an impaired susceptibility to HIV-1 infection. A marked down-modulation of surface CD4 receptors was detected in Vpr-expressing clones with respect to control cells. Likewise, a reduced CD4 expression was als...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.74.21.10207-10211.2000
更新日期：2000-11-01 00:00:00
abstract：:Adenovirus (Ad) entry into cells is initiated by the binding of the fiber knob to a cell surface receptor. The coxsackie- and adenovirus receptor (CAR) functions as the attachment receptor for many, but not all, Ad serotypes. Ad type 37 (Ad37), a subgroup D virus that causes keratoconjunctivitis in humans, does not in...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.75.11.5375-5380.2001
更新日期：2001-06-01 00:00:00
abstract：:Myeloblastosis-associated virus type 1 (MAV1) derived from a molecular clone of infectious proviral DNA (B. Perbal, J. S. Lipsick, J. Svoboda, R. F. Silva, and M. A. Baluda, J. Virol. 56:240-244, 1985) was shown to specifically induce nephroblastoma in chickens and therefore belongs to the MAV-N class. We show that ne...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.63.4.1803-1807.1989
更新日期：1989-04-01 00:00:00
abstract：:Cytotoxic T lymphocytes (CTLs) are important for the control of virus replication during respiratory infections. For human metapneumovirus (hMPV), an H-2(d)-restricted CTL epitope in the M2-2 protein has been described. In this study, we screened the hMPV F, G, N, M, M2-1, and M2-2 proteins using three independent alg...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.02423-06
更新日期：2007-10-01 00:00:00
abstract：:The neutralizing activities of polyclonal antibodies and monoclonal antibodies (MAbs) obtained by immunization of mice with L1 virus-like particles (VLPs) were investigated by using pseudovirion infectivity assays for human papillomavirus type 16 (HPV-16), HPV-31, HPV-33, HPV-45, HPV-58, and HPV-59 to obtain a better ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.76.13.6480-6486.2002
更新日期：2002-07-01 00:00:00
abstract：:Inclusion bodies are a characteristic feature of ebolavirus infections in cells. They contain large numbers of preformed nucleocapsids, but their biological significance has been debated, and they have been suggested to be aggregates of viral proteins without any further biological function. However, recent data for o...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01525-12
更新日期：2012-11-01 00:00:00
abstract：:The RNA-dependent RNA polymerase (NS5B) of hepatitis C virus (HCV) is an unusually attractive target for drug discovery since it contains five distinct drugable sites. The success of novel antiviral therapies will require nonnucleoside inhibitors to be active in at least patients infected with HCV of subtypes 1a and 1...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.01980-09
更新日期：2010-03-01 00:00:00
abstract：:Poliovirus proteinase 2A (2Apro) is autocatalytically released from the viral polyprotein by cleavage in cis of a Tyr-Gly dipeptide at its own amino terminus, resulting in separation of the P1 structural and P2-P3 nonstructural protein precursors. A second Ty-Gly dipeptide within 3D polymerase is cleaved by 2Apro in t...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.66.6.3330-3338.1992
更新日期：1992-06-01 00:00:00
abstract：:Human immunodeficiency virus type 1 (HIV-1) exists as a complex population of multiple genotypic variants in persons with chronic infection. However, acute HIV-1 infection via sexual transmission is a low-probability event in which there is thought to be low genetic complexity in the initial inoculum. In order to asse...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.78.20.11208-11218.2004
更新日期：2004-10-01 00:00:00
abstract：:Several groups have reported that certain herpesvirus envelope proteins do not remain on the surface of cells that express them but rather are internalized by endocytosis in a recycling process. The biological function of membrane protein endocytosis in the virus life cycle remains a matter of speculation and debate. ...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.72.6.4571-4579.1998
更新日期：1998-06-01 00:00:00
abstract：:Translation of encephalomyocarditis virus RNA in rabbit reticulocyte lysates in the presence of N-formyl-[(35)S]methionine-tRNA(f) (Met) revealed that a small polypeptide is cleaved from the N-terminus of the capsid protein precursor, preA, by virus-coded protease activity. Therefore, this N-terminal segment comprisin...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/JVI.45.1.439-441.1983
更新日期：1983-01-01 00:00:00
abstract：:The initial aim of this study was to combine attributes of adeno-associated virus (AAV) and adenovirus (Ad) gene therapy vectors to generate an Ad-AAV hybrid vector allowing efficient site-specific integration with Ad vectors. In executing our experimental strategy, we found that, in addition to the known incompatibil...
journal_title：Journal of virology
pub_type： 杂志文章
doi：10.1128/jvi.76.11.5411-5421.2002
更新日期：2002-06-01 00:00:00
