Abstract:
:The distribtuion of various amino acid tRNA's in the 4S RNA components of avian myeloblastosis virus (AMV) and in 4S RNA prepared from chicken cmbryo cells, chicken myeloblasts, and chicken livers was determined. This was done by aminoacylating the 4S RNA samples with a mixture of 17 radioactive amino acids and subsequently identifying the tRNA-accepted amino acids on an amino acid analyzer after deacylation. In embryo cells, myeloblasts, and liver, tRNA's accepting all 1m amino acids were demonstrated. "Free" AMV 4S RNA was characterized by very low quantities of glutamate, valine, and tyrosine tRNA's. RNAs accepting all 17 amino acids, with the exception of tyrosine, were shown to be present in the "70S-associated" 4S RNA which dissociates at 60 C. The bulk of the 70S-associated 4S RNA was dissociated at 60 C at low ionic strength with a concomitant conversion of 70S RNA to 35S RNA. The residual associated 4S RNA was dissociated by further heating of the 35S RNA to 80 C; tryptophan tRNA accounted for greater than 90% of the total amino acid accepting activity in this fraction. The results support other studies in suggesting that tryptophan tRNA may serve as a primer for DNA synthesis in AMV, as has been shown in Rous sarcoma virus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Waters LC,Mullin BC,Bailiff EG,Popp RAdoi
10.1128/JVI.16.6.1608-1614.1975subject
Has Abstractpub_date
1975-12-01 00:00:00pages
1608-14issue
6eissn
0022-538Xissn
1098-5514journal_volume
16pub_type
杂志文章abstract::The efficient attachment of pseudorabies virus to cultured cells is dependent on an electrostatic interaction between negatively charged cell surface heparan sulfate and the viral envelope glycoprotein gC. Deletion of the first one-third of gC severely impairs virus attachment, but the mutant virions are still capable...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.834-839.1995
更新日期:1995-02-01 00:00:00
abstract::The 5' untranslated region (UTR) of hepatitis C virus (HCV), which is composed of four domains (I, II, III, and IV) and a pseudoknot, is essential for translation and viral replication. Equine nonprimate hepacivirus (EHcV) harbors a 5' UTR consisting of a large 5'-terminal domain (I); three additional domains (I', II,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01997-17
更新日期:2018-03-14 00:00:00
abstract::Recently, recombinant baculoviruses have been used to show that expression of six herpes simplex virus type 1 genes results in the formation of capsid-like particles. We have applied cryoelectron microscopy and three-dimensional image reconstruction to establish their structural authenticity to a resolution of approxi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7362-7366.1995
更新日期:1995-11-01 00:00:00
abstract::We have previously designed in vitro model systems to characterize the herpes simplex virus type 1 (HSV-1) genome during in vitro virus latency. Latency was established by treatment of infected human embryo lung fibroblast (HEL-F) cells or rat fetal neurons with (E)-5-(2-bromovinyl)-2'-deoxyuridine and human leukocyte...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.1.205-213.1984
更新日期:1984-01-01 00:00:00
abstract:UNLABELLED:Herpes simplex virus 1 (HSV-1) and HSV-2 are among the most prevalent human pathogens. Both viruses can recognize, via the surface envelope glycoprotein D (gD), human nectin-1 as a functional receptor. Previous studies have successfully elucidated the molecular basis of the binding between HSV-1 gD and necti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01906-14
更新日期:2014-12-01 00:00:00
abstract::Changes in the envelope glycoprotein ectodomains of a nonpathogenic simian-human immunodeficiency virus (SHIV-89.6) that was serially passaged in vivo have been shown to be responsible for the increased pathogenicity of the resulting virus, SHIV-KB9 (G. B. Karlsson, et al., J. Exp. Med. 188:1159-1171, 1998). The 12 am...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4433-4440.2000
更新日期:2000-05-01 00:00:00
abstract::The human cyclin T1 (hCycT1) protein from the positive transcription elongation factor b (P-TEFb) binds the transactivator Tat and the transactivation response (TAR) RNA stem loop from human immunodeficiency virus type 1 (HIV). This complex activates the elongation of viral transcription. To create effective inhibitor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.21.10873-10881.2002
更新日期:2002-11-01 00:00:00
abstract::Molecularly cloned proviral DNA of avian oncogenic retrovirus CMII was isolated by screening a genomic library of a CMII-transformed quail cell line with a myc-specific probe. On a 10.4-kilobase EcoRI fragment, the cloned DNA contained 4.4 kilobases of CMII proviral sequences extending from the 5' long terminal repeat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.2.576-585.1985
更新日期:1985-05-01 00:00:00
abstract::The recent identification of coreceptors that mediate efficient entry of human immunodeficiency virus type 1 (HIV-1) suggests new therapeutic and preventive strategies. We analyzed simian immunodeficiency virus (SIV) entry cofactors to investigate whether the macaque SIV model can be used as an experimental model to e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6509-6516.1997
更新日期:1997-09-01 00:00:00
abstract::Human cytomegalovirus (HCMV) has evolved multiple strategies for suppression of the antiviral response of the infected cell. DNA array technology has revealed that HCMV clearly regulates host gene expression during the course of a productive infection by enhancing, sustaining, or suppressing steady-state levels of cel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.472-485.2005
更新日期:2005-01-01 00:00:00
abstract::Two foot-and-mouth disease virus (FMDV) genome sequences have been determined for isolates collected from recent field outbreaks in North Africa (Egypt) and the Middle East (Palestinian Autonomous Territories). These data represent the first examples of complete genomic sequences for the FMDV SAT 2 topotype VII, which...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01231-12
更新日期:2012-08-01 00:00:00
abstract::The time course and extent of mengovirus production were the same in metaphase-arrested and interphase L-cells, yet the expression of cytopathology was delayed several hours in metaphase cells. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.2.262-263.1970
更新日期:1970-02-01 00:00:00
abstract::Northern (RNA) blot analysis was used to determine the spatial and temporal distribution of bovine herpesvirus 1 (BHV-1) transcripts. Total RNA was isolated from Madin-Darby bovine kidney cells which had been infected with BHV-1.2b strain K22 or BHV-1.1 strain Jura in the presence or absence of metabolic inhibitors. C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4882-4889.1989
更新日期:1989-11-01 00:00:00
abstract::The Arabidopsis flowering locus T (FT) gene encodes the mobile florigen essential for floral induction. While movement of the FT protein has been shown to occur within plants, systemic spread of FT mRNA remains to be unequivocally demonstrated. Utilizing novel RNA mobility assay vectors based on two distinct movement-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02346-08
更新日期:2009-04-01 00:00:00
abstract::We characterized a number of important features of the structure of the cohesive overlap region of the DNA genome of duck hepatitis B virus. The 5'-terminal nucleotide of minus-strand DNA was localized to nucleotide 2537, a G residue within the 12-base repeat sequence DR1. This G residue was shown to be the site of a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
abstract::The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00356-15
更新日期:2015-08-01 00:00:00
abstract::In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.10.5535-5538.1991
更新日期:1991-10-01 00:00:00
abstract::Human immunodeficiency virus type 1 contains a transmembrane glycoprotein with an unusually long cytoplasmic domain. To determine the role of this domain in virus replication, a series of single nucleotide changes that result in the insertion of premature termination codons throughout the cytoplasmic domain has been c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6616-6625.1992
更新日期:1992-11-01 00:00:00
abstract::The envelope protein E of flaviviruses mediates both receptor-binding and membrane fusion. At the virion surface, 180 copies of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in noninfectious subviral particles, 60 copies are arranged in a T=1 icosahedral symmetry. In both case...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00660-09
更新日期:2009-09-01 00:00:00
abstract::Upon cell entry, the genomes of herpes simplex virus type 1 (HSV-1) and adenovirus (Ad) associate with distinct nuclear structures termed ND10 or promyelocytic leukemia (PML) nuclear bodies (NBs). PML NB morphology is altered or disrupted by specific viral proteins as replication proceeds. We examined whether adeno-as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.1.389-398.2004
更新日期:2004-01-01 00:00:00
abstract::Replication and transcription activator (RTA) (also referred to as ORF50), an immediate-early gene product of Kaposi's sarcoma-associated herpesvirus (KSHV)/(human herpesvirus 8), plays a critical role in balancing the viral life cycle between latency and lytic replication. RTA has been shown to act as a strong transc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.17.9451-9462.2003
更新日期:2003-09-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) is a 1,162-amino-acid protein that mediates episome persistence of viral genomes. LANA binds the KSHV terminal-repeat (TR) sequence through its carboxy-terminal domain to mediate DNA replication. LANA simultaneously binds mitotic ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02158-18
更新日期:2019-03-05 00:00:00
abstract:UNLABELLED:To investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) regulatory protein ICP0 promotes viral gene expression and replication, we screened cells overexpressing ICP0 for ICP0-binding host cell proteins. Tandem affinity purification of transiently expressed ICP0 coupled with mass spe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03043-15
更新日期:2016-01-06 00:00:00
abstract:UNLABELLED:Due to a scarcity of immunocompetent animal models for viral hepatitis, little is known about the early innate immune responses in the liver. In various hepatotoxic models, both pro- and anti-inflammatory activities of recruited monocytes have been described. In this study, we compared the effect of liver in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03733-14
更新日期:2015-05-01 00:00:00
abstract::The herpes simplex virus type 1 latency-associated transcript (LAT) inhibits apoptosis. We demonstrate here that LAT influences the accumulation of the Bcl-x(L) transcript versus the Bcl-x(S) transcript in Neuro-2A cells. Bcl-x(L) encodes an antiapoptotic protein, whereas Bcl-x(S) encodes a proapoptotic protein. Promo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10714-10718.2003
更新日期:2003-10-01 00:00:00
abstract::Defining a macrophage-tropic phenotype for HIV-1 to assess a role in pathogenesis is complicated by the fact that HIV-1 isolates vary continuously in their ability to enter monocyte-derived macrophages (MDMs) in vitro, and MDMs vary in their ability to support HIV-1 entry. To overcome these limitations, we identified ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02477-13
更新日期:2014-02-01 00:00:00
abstract::In clinical trials, HIV-1 broadly neutralizing antibodies (bnAbs) effectively lower plasma viremia and delay virus reemergence. The presence of less neutralization-susceptible strains prior to treatment decreases the efficacy of these antibody-based treatments, but neutralization sensitivity often cannot be predicted ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01604-19
更新日期:2020-01-06 00:00:00
abstract::Profiles of bacteriophage T4 early proteins resolved by a two-dimensional nonequilibrium pH gradient electrophoresis system (P. Z. O'Farrell, H. M. Goodman, and P. H. O'Farrell, Cell 12:1133--1142, 1977) are presented. Over 65 phage-induced proteins were resolved. Amber or deletion mutants were used to identify 17 pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.42.3.767-772.1982
更新日期:1982-06-01 00:00:00
abstract::Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00046-06
更新日期:2006-06-01 00:00:00
abstract::An adenovirus vector encoding murine Fas ligand (mFasL) under an inducible control was derived. In vivo ectopic expression of mFasL in murine livers induced an inflammatory cellular infiltration. Furthermore, ectopic expression of mFasL by myocytes did not allow prolonged vector-mediated transgene expression. Thus, ec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2483-2490.1998
更新日期:1998-03-01 00:00:00