Abstract:
:The envelope protein E of flaviviruses mediates both receptor-binding and membrane fusion. At the virion surface, 180 copies of E are tightly packed and organized in a herringbone-like icosahedral structure, whereas in noninfectious subviral particles, 60 copies are arranged in a T=1 icosahedral symmetry. In both cases, the basic building block is an E dimer which exposes the binding sites for neutralizing antibodies at its surface. It was the objective of our study to assess the dependence of the antigenic structure of E on its quaternary arrangement, i.e., as part of virions, recombinant subviral particles, or soluble dimers. For this purpose, we used a panel of 11 E protein-specific neutralizing monoclonal antibodies, mapped to distinct epitopes in each of the three E protein domains, and studied their reactivity with the different soluble and particulate forms of tick-borne encephalitis virus E protein under nondenaturing immunoassay conditions. Significant differences in the reactivities with these forms were observed that could be related to (i) limited access of certain epitopes at the virion surface; (ii) limited occupancy of epitopes in virions due to steric hindrance between antibodies; (iii) differences in the avidity to soluble forms compared to the virion, presumably related to the flexibility of E at its domain junctions; and (iv) modulations of the external E protein surface through interactions with its stem-anchor structure. We have thus identified several important factors that influence the antigenicity of the flavivirus E protein and have an impact on the interaction with neutralizing antibodies.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kiermayr S,Stiasny K,Heinz FXdoi
10.1128/JVI.00660-09subject
Has Abstractpub_date
2009-09-01 00:00:00pages
8482-91issue
17eissn
0022-538Xissn
1098-5514pii
JVI.00660-09journal_volume
83pub_type
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doi:10.1128/JVI.46.3.1051-1055.1983
更新日期:1983-06-01 00:00:00
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doi:10.1128/jvi.74.1.228-236.2000
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更新日期:1971-12-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.49.1.300-301.1984
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.2.782-786.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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更新日期:2003-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1969-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.34.2.550-559.1980
更新日期:1980-05-01 00:00:00
abstract::Coxsackievirus B3 (CVB3) is known to infect stem cells in the neonatal central nervous system. Here, we evaluated the effects of CVB3 infection on the major source and repository of stem cells, the bone marrow (BM). Viral genome was detectable in BM within 24 h of infection, and productive infection of BM cells was ev...
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pub_type: 杂志文章
doi:10.1128/JVI.03004-12
更新日期:2013-03-01 00:00:00