Abstract:
:Infection of normal rat fibroblasts (FR 3T3) with the early tsa mutant of polyoma virus may lead to either the A or the N phenotype, tsa-A transformants, originally derived by agar selection, are not temperature dependent for maintenance of the transformed phenotype, whereas tsa-N transormants revert at high temperature to normal growth control. A transformants did not result from an independent cellular mutation selected in agar medium, but rather from a transformation process distinct from that leading to the N state. It occurred in both liquid and agar media when the infected cells were maintained under growth-restricting conditions, such as absence of anchorage and contact inhibition at confluency. N transformation occurred in cells maintained in active growth after virus infection (sparse cultures on a solid substratum). Physiological conditions during a critical period after virus infection thus appear to be a crucial parameter of the transformation process.
journal_name
J Viroljournal_title
Journal of virologyauthors
Rassoulzadegan M,Seif R,Cuzin Fdoi
10.1128/JVI.28.2.421-426.1978subject
Has Abstractpub_date
1978-11-01 00:00:00pages
421-6issue
2eissn
0022-538Xissn
1098-5514journal_volume
28pub_type
杂志文章abstract::Prasinoviruses infecting unicellular green algae in the order Mamiellales (class Mamiellophyceae) are commonly found in coastal marine waters where their host species frequently abound. We tested 40 Ostreococcus tauri viruses on 13 independently isolated wild-type O. tauri strains, 4 wild-type O. lucimarinus strains, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.07221-11
更新日期:2012-04-01 00:00:00
abstract::The vast majority of people already have preexisting immune responses to influenza viruses from one or more subtypes. However, almost all preclinical studies evaluate new influenza vaccine candidates in immunologically naive animals. Recently, our group demonstrated that priming naive ferrets with broadly reactive H1 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-18
更新日期:2019-01-17 00:00:00
abstract::Human respiratory syncytial virus (RSV) is a serious respiratory pathogen in infants and young children as well as elderly and immunocompromised populations. However, no RSV vaccines are available. We have explored the potential of virus-like particles (VLPs) as an RSV vaccine candidate. VLPs composed entirely of RSV ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01861-10
更新日期:2011-01-01 00:00:00
abstract::Hepatitis C virus (HCV) exploits cellular proteins to facilitate viral propagation. To identify the cellular factors involved in the HCV life cycle, we previously performed protein microarray assays using either HCV nonstructural 5A (NS5A) protein or core protein as a probe. Interestingly, cellular cortactin strongly ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01306-20
更新日期:2020-09-15 00:00:00
abstract::Previous studies have suggested that the U(L)17 gene of herpes simplex virus type 1 (HSV-1) is essential for virus replication. In this study, viral mutants incorporating either a lacZ expression cassette in place of 1,490 bp of the 2,109-bp U(L)17 open reading frame [HSV-1(deltaU(L)17)] or a DNA oligomer containing a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3779-3788.1998
更新日期:1998-05-01 00:00:00
abstract::Venezuelan equine encephalitis (VEE) virus antigenic subtypes and varieties are considered either epidemic/epizootic or enzootic. In addition to epidemiological differences between the epidemic and enzootic viruses, several in vitro and in vivo laboratory markers distinguishing the viruses have been identified, includ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.9.4258-4263.2000
更新日期:2000-05-01 00:00:00
abstract::Human CD317 (BST-2/tetherin) is an intrinsic immunity factor that blocks the release of retroviruses, filoviruses, herpesviruses, and arenaviruses. It is unclear whether CD317 expressed endogenously in rodent cells has the capacity to interfere with the replication of the retroviral rodent pathogen murine leukemia vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01067-10
更新日期:2010-11-01 00:00:00
abstract::We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.291-298.1978
更新日期:1978-05-01 00:00:00
abstract::Lymphocyte antigen 6E (LY6E) is a GPI-anchored, interferon-inducible protein that has been shown to modulate viral infection in a cell type-dependent manner. Our recent work showed that LY6E promotes HIV-1 infection in some high-CD4-expressing cells, including human peripheral blood mononuclear cells (PBMCs) and the S...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01866-18
更新日期:2019-03-21 00:00:00
abstract::Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3904-3908.1992
更新日期:1992-06-01 00:00:00
abstract::Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02081-12
更新日期:2012-12-01 00:00:00
abstract::The inducible transcription factor NF-kappaB is commonly activated upon RNA virus infection and is a key player in the induction and regulation of the innate immune response. Borna disease virus (BDV) is a neurotropic negative-strand RNA virus, which replicates in the nucleus of the infected cell and causes a persiste...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6043-6051.2005
更新日期:2005-05-01 00:00:00
abstract::Cellular and viral factors participate in the replication cycle of rotavirus. We report that the guanine nucleotide exchange factor GBF1, which activates the small GTPase Arf1 to induce COPI transport processes, is required for rotavirus replication since knocking down GBF1 expression by RNA interference or inhibiting...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01062-19
更新日期:2019-09-12 00:00:00
abstract::Cytolytic T-lymphocyte (CTL) activity specific for respiratory syncytial (RS) virus was investigated after intranasal infection of mice with RS virus, after intraperitoneal infection of mice with a recombinant vaccinia virus expressing the F glycoprotein, and after intramuscular vaccination of mice with Formalin-inact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4232-4241.1990
更新日期:1990-09-01 00:00:00
abstract::Influenza A viruses of the H9N2 subtype are endemic in poultry in many Eurasian countries and have occasionally caused clinical respiratory diseases in humans. While some avian H9N2 viruses have glutamine (Q) at amino acid position 226 of the hemagglutinin (HA) receptor-binding site, an increasing number of isolates h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02827-06
更新日期:2007-05-01 00:00:00
abstract::The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3740-3748.1992
更新日期:1992-06-01 00:00:00
abstract::Similar to other positive-sense, single-stranded RNA viruses, hepatitis C virus (HCV) replicates its genome in a remodeled intracellular membranous structure known as the membranous web (MW). To date, the process of MW formation remains unclear. It is generally acknowledged that HCV nonstructural protein 4B (NS4B) can...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01785-12
更新日期:2012-12-01 00:00:00
abstract::Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1647-1650.1987
更新日期:1987-05-01 00:00:00
abstract::Efforts to develop adenovirus vectors suitable for genetic interventions in humans have identified three major limitations of the most frequently used vector prototype, human adenovirus serotype 5 (Ad5). These limitations--widespread preexisting anti-Ad5 immunity in humans, the high rate of transduction of normal nont...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02425-09
更新日期:2010-10-01 00:00:00
abstract::Productive replication of human immunodeficiency virus type 1 (HIV-1) occurs efficiently only in humans. The posttranscriptional stages of the HIV-1 life cycle proceed poorly in mouse cells, with a resulting defect in viral assembly and release. Previous work has shown that the presence of human chromosome 2 increases...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01970-12
更新日期:2012-11-01 00:00:00
abstract::Adeno-associated virus (AAV) is a single-stranded DNA virus with a unique biphasic lifestyle consisting of both a productive and a latent phase. Typically, the productive phase requires coinfection with a helper virus, for instance adenovirus, while the latent phase dominates in healthy cells. In the latent state, AAV...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.15.7874-7882.2004
更新日期:2004-08-01 00:00:00
abstract::The major structural glycoprotein E2 of classical swine fever virus (CSFV) is responsible for eliciting neutralizing antibodies and conferring protective immunity. The current structural model of this protein predicts its surface-exposed region at the N terminus with a short stretch of the C-terminal residues spanning...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11619-11625.2000
更新日期:2000-12-01 00:00:00
abstract::The buoyant density characteristics of infectious particles of herpes simplex virus types 1 and 2 were studied by centrifugation in sucrose and cesium chloride density gradients with a high resolution and satisfactory infectivity recovery. It was shown that two populations of infectious virions differing in buoyant de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.38.2.737-748.1981
更新日期:1981-05-01 00:00:00
abstract::We have reported previously that ocular infection of different strains of mice with recombinant herpes simplex virus 1 (HSV-1) constitutively expressing interleukin-2 (IL-2) provokes central nervous system (CNS) demyelination and optic neuropathy, as determined by changes in visual evoked cortical potentials and patho...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02241-13
更新日期:2013-11-01 00:00:00
abstract::The efficiency of processing of polyoma viral RNA and of its export from nucleus to cytoplasm was measured in primary mouse kidney cells by comparing the initial rates of incorporation of [3H]uridine into cytoplasmic and nuclear viral RNA. Appropriate methods of cell fractionation were chosen to maximize yields of cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.628-635.1981
更新日期:1981-02-01 00:00:00
abstract::An mRNA-ribonucleoprotein particle (mRNP) was found in vesicular stomatitis virus (VSV)-infected Chinese hamster ovary cells. The particle was present 3 and 4.5 h after infection but was barely discernible at 2 h. The mRNP (buoyant density, 1.56 g/cm3), which cosedimented with viral nucleocapsid in a sucrose density g...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.932-938.1982
更新日期:1982-12-01 00:00:00
abstract::Adenovirus infection and expression of E1A induces both proliferation and apoptosis, the latter of which is blocked by the adenovirus Bcl-2 homologue E1B 19K. The mechanism of apoptosis induction and the role that it plays in productive infection are not known. Unlike apoptosis mediated by death receptors, infection w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.9.4547-4558.2002
更新日期:2002-05-01 00:00:00
abstract::Viruses encode proteins that disrupt chemokine responses. The murine gammaherpesvirus 68 gene M3 encodes a chemokine binding protein (vCKBP-3) which has no sequence similarity to chemokine receptors but inhibits chemokine receptor binding and activity. We have used a panel of CXCL8 analogs to identify the structural r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8588-8592.2003
更新日期:2003-08-01 00:00:00
abstract::Noroviruses (NoVs) are a leading cause of gastroenteritis worldwide, yet host factors that restrict NoV replication are not well understood. Here, we use a CRISPR activation genome-wide screening to identify host genes that can inhibit murine norovirus (MNoV) replication in human cells. Our screens identified with hig...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01324-18
更新日期:2018-12-10 00:00:00
abstract::The sole immediate-early (IE) gene of equine herpesvirus 1 encodes a 1,487-amino-acid (aa) regulatory phosphoprotein that independently activates expression of early viral genes. Coimmunoprecipitation assays demonstrated that the IE protein physically interacts with the general transcription factor TFIIB. Using a vari...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.21.10219-10230.2001
更新日期:2001-11-01 00:00:00