Genetic subpopulations of Rift Valley fever virus strains ZH548 and MP-12 and recombinant MP-12 strains.

Abstract:

:Rift Valley fever virus strain MP-12 was generated by serial plaque passages of parental strain ZH548 12 times in MRC-5 cells in the presence of a chemical mutagen, 5-fluorouracil. As a result, MP-12 encoded 4, 9, and 10 mutations in the S, M, and L segments, respectively. Among them, mutations in the M and L segments were responsible for attenuation, while the MP-12 S segment still encoded a virulent phenotype. We performed high-throughput sequencing of MP-12 vaccine, ZH548, and recombinant MP-12 (rMP-12) viruses. We found that rMP-12 contains very low numbers of viral subpopulations, while MP-12 and ZH548 contain 2 to 4 times more viral genetic subpopulations than rMP-12. MP-12 genetic subpopulations did not encode the ZH548 sequence at the 23 MP-12 consensus mutations. On the other hand, 4 and 2 mutations in M and L segments of MP-12 were found in ZH548 subpopulations. Thus, those 6 mutations were no longer MP-12-specific mutations. ZH548 encoded several unique mutations compared to other Egyptian strains, i.e., strains ZH501, ZH1776, and ZS6365. ZH548 subpopulations shared nucleotides at the mutation site common with those in the Egyptian strains, while MP-12 subpopulations did not share those nucleotides. Thus, MP-12 retains unique genetic subpopulations and has no evidence of reversion to the ZH548 sequence in the subpopulations. This study provides the first information regarding the genetic subpopulations of RVFV and shows the genetic stability of the MP-12 vaccine manufactured in MRC-5 cells.

journal_name

J Virol

journal_title

Journal of virology

authors

Lokugamage N,Freiberg AN,Morrill JC,Ikegami T

doi

10.1128/JVI.02081-12

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

13566-75

issue

24

eissn

0022-538X

issn

1098-5514

pii

JVI.02081-12

journal_volume

86

pub_type

杂志文章
  • Adenovirus Core Protein VII Downregulates the DNA Damage Response on the Host Genome.

    abstract::Viral manipulation of cellular proteins allows viruses to suppress host defenses and generate infectious progeny. Due to the linear double-stranded DNA nature of the adenovirus genome, the cellular DNA damage response (DDR) is considered a barrier to successful infection. The adenovirus genome is packaged with protein...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01089-17

    authors: Avgousti DC,Della Fera AN,Otter CJ,Herrmann C,Pancholi NJ,Weitzman MD

    更新日期:2017-09-27 00:00:00

  • Dexamethasone stimulates expression of transposable type A intracisternal retroviruslike genes in mouse (Mus musculus) cells.

    abstract::Dexamethasone treatment enhances expression of transposable intracisternal type A particles (IAP) at RNA and proteins levels in a murine retrovirus-transformed cell line (Ki-BALB). This effect was ascertained by electron microscopic numeration of IAP. By sequence comparison, we located glucocorticoid-responsive elemen...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.62.10.3867-3869.1988

    authors: Emanoil-Ravier R,Mercier G,Canivet M,Garcette M,Lasneret J,Peronnet F,Best-Belpomme M,Peries J

    更新日期:1988-10-01 00:00:00

  • Analysis of the herpes simplex virus genome during in vitro latency in human diploid fibroblasts and rat sensory neurons.

    abstract::We have previously designed in vitro model systems to characterize the herpes simplex virus type 1 (HSV-1) genome during in vitro virus latency. Latency was established by treatment of infected human embryo lung fibroblast (HEL-F) cells or rat fetal neurons with (E)-5-(2-bromovinyl)-2'-deoxyuridine and human leukocyte...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.49.1.205-213.1984

    authors: Wigdahl B,Scheck AC,Ziegler RJ,De Clercq E,Rapp F

    更新日期:1984-01-01 00:00:00

  • Lentiviral vector-based prime/boost vaccination against AIDS: pilot study shows protection against Simian immunodeficiency virus SIVmac251 challenge in macaques.

    abstract::AIDS vaccination has a pressing need for more potent vaccination vectors capable of eliciting strong, diversified, and long-lasting cellular immune responses against human immunodeficiency virus (HIV). Lentiviral vectors have demonstrated efficiency not only as gene delivery vehicles for gene therapy applications but ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01284-09

    authors: Beignon AS,Mollier K,Liard C,Coutant F,Munier S,Rivière J,Souque P,Charneau P

    更新日期:2009-11-01 00:00:00

  • Full-Length Glycosylated Gag of Murine Leukemia Virus Can Associate with the Viral Envelope as a Type I Integral Membrane Protein.

    abstract::The glycosylated Gag protein (gPr80) of murine leukemia viruses (MLVs) has been shown to exhibit multiple roles in facilitating retrovirus release, infection, and resistance to host-encoded retroviral restriction factors, such as APOBEC3, SERINC3, and SERINC5. One way in which gPr80 helps MLVs to escape host innate im...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01530-17

    authors: Renner TM,Bélanger K,Lam C,Gerpe MCR,McBane JE,Langlois MA

    更新日期:2018-02-26 00:00:00

  • Nef-induced major histocompatibility complex class I down-regulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation.

    abstract::The N-terminal alpha-helix domain of the human immunodeficiency virus type 1 (HIV-1) Nef protein plays important roles in enhancement of viral infectivity, virion incorporation of Nef, and the down-regulation of major histocompatibility complex class I (MHC-I) expression on cell surfaces. In this study, we demonstrate...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.74.6.2907-2912.2000

    authors: Akari H,Arold S,Fukumori T,Okazaki T,Strebel K,Adachi A

    更新日期:2000-03-01 00:00:00

  • Low probability of double integration in transformation of nonpermissive cells by polyomavirus.

    abstract::The fate of polyomavirus genomes in stable transformants was examined in experiments in which rat or hamster cells were infected with mixed viral populations containing either two distinguishable wild types or a wild type plus a transformation-deficient mutant. The results demonstrate that in 90% of the cases in eithe...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.64.3.1304-1313.1990

    authors: Oh SY,Amalfitano A,Friderici K,Chen MC,Fluck MM

    更新日期:1990-03-01 00:00:00

  • Binding and relocalization of protein kinase R by murine cytomegalovirus.

    abstract::Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kinase R (PKR). In the case of murine cytomegalovirus (MCMV), the protein products of two essential genes, m142 and m143, bind to double-stranded RNA (dsRNA) and block phosphorylation of PKR and eukaryotic initiation factor...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01484-08

    authors: Child SJ,Geballe AP

    更新日期:2009-02-01 00:00:00

  • Replication of Small Ruminant Lentiviruses in Aluminum Hydroxide-Induced Granulomas in Sheep: a Potential New Factor for Viral Dissemination.

    abstract::Aluminum (Al)-based salts are widely used adjuvants in ruminants and other species to strengthen the immune response elicited against vaccine antigen(s). However, they can lead to the formation of long-lasting granulomas composed of abundant activated macrophages. Small ruminant lentiviruses (SRLV) are widely distribu...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01859-20

    authors: Echeverría I,de Miguel R,Asín J,Rodríguez-Largo A,Fernández A,Pérez M,de Andrés D,Luján L,Reina R

    更新日期:2020-12-22 00:00:00

  • The ABCs of rhinoviruses, wheezing, and asthma.

    abstract::Human rhinoviruses (HRVs) were discovered as common cold pathogens over 50 years ago. Recent advances in molecular viral diagnostics have led to an appreciation of their role in more-significant respiratory illnesses, including bronchiolitis in infancy, childhood pneumonia, and acute exacerbations of chronic respirato...

    journal_title:Journal of virology

    pub_type: 杂志文章,评审

    doi:10.1128/JVI.02290-09

    authors: Gern JE

    更新日期:2010-08-01 00:00:00

  • Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses.

    abstract::The rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed PathogenID, which was designed for multiple pathogen detection using ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00771-10

    authors: Dacheux L,Berthet N,Dissard G,Holmes EC,Delmas O,Larrous F,Guigon G,Dickinson P,Faye O,Sall AA,Old IG,Kong K,Kennedy GC,Manuguerra JC,Cole ST,Caro V,Gessain A,Bourhy H

    更新日期:2010-09-01 00:00:00

  • Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells.

    abstract::The cytomegalovirus (CMV) serine proteinase assemblin is synthesized as a precursor that undergoes three principal autoproteolytic cleavages. Two of these are common to the assemblin homologs of all herpes group viruses: one at the maturational site near the carboxyl end of the precursor and another at the release sit...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.70.8.5395-5404.1996

    authors: Hall MR,Gibson W

    更新日期:1996-08-01 00:00:00

  • Herpes simplex virus tegument protein VP22 contains an internal VP16 interaction domain and a C-terminal domain that are both required for VP22 assembly into the virus particle.

    abstract::Many steps along the herpesvirus assembly and maturation pathway remain unclear. In particular, the acquisition of the virus tegument is a poorly understood process, and the molecular interactions involved in tegument assembly have not yet been defined. Previously we have shown that the two major herpes simplex virus ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.20.13082-13093.2005

    authors: Hafezi W,Bernard E,Cook R,Elliott G

    更新日期:2005-10-01 00:00:00

  • Phenotypic progression of a rat lymphoid cell line immortalized by human T-lymphotropic virus type I to induce lymphoma/leukemia-like disease in rats.

    abstract::Rat lymphoid cells, TARS-1, immortalized by coculture with adult T-cell leukemia cells, were intraperitoneally injected into 65 newborn, inbred WKAH/Hkm rats. In most of the rats, tumor nodules were discernible 7 to 15 days after transplantation but were completely rejected within 5 to 6 weeks. Two rats with no tumor ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.66.11.6686-6694.1992

    authors: Oka T,Sonobe H,Iwata J,Kubonishi I,Satoh H,Takata M,Tanaka Y,Tateno M,Tozawa H,Mori S

    更新日期:1992-11-01 00:00:00

  • The Human T-Cell Leukemia Virus Type 1 Basic Leucine Zipper Factor Attenuates Repair of Double-Stranded DNA Breaks via Nonhomologous End Joining.

    abstract::Adult T-cell leukemia (ATL) is a fatal malignancy of CD4+ T cells infected with human T-cell leukemia virus type 1 (HTLV-1). ATL cells often exhibit random gross chromosomal rearrangements that are associated with the induction and improper repair of double-stranded DNA breaks (DSBs). The viral oncoprotein Tax has bee...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00672-18

    authors: Rushing AW,Hoang K,Polakowski N,Lemasson I

    更新日期:2018-07-17 00:00:00

  • Direct sequencing and characterization of a clinical isolate of Epstein-Barr virus from nasopharyngeal carcinoma tissue by using next-generation sequencing technology.

    abstract::Epstein-Barr virus (EBV)-encoded molecules have been detected in the tumor tissues of several cancers, including nasopharyngeal carcinoma (NPC), suggesting that EBV plays an important role in tumorigenesis. However, the nature of EBV with respect to genome width in vivo and whether EBV undergoes clonal expansion in th...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.00823-11

    authors: Liu P,Fang X,Feng Z,Guo YM,Peng RJ,Liu T,Huang Z,Feng Y,Sun X,Xiong Z,Guo X,Pang SS,Wang B,Lv X,Feng FT,Li DJ,Chen LZ,Feng QS,Huang WL,Zeng MS,Bei JX,Zhang Y,Zeng YX

    更新日期:2011-11-01 00:00:00

  • Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by beta3 integrins.

    abstract::Hantaviruses replicate primarily in the vascular endothelium and cause two human diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). In this report, we demonstrate that the cellular entry of HFRS-associated hantaviruses is facilitated by specific integrins expressed on plate...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.73.5.3951-3959.1999

    authors: Gavrilovskaya IN,Brown EJ,Ginsberg MH,Mackow ER

    更新日期:1999-05-01 00:00:00

  • Mechanism of leukemogenesis induced by mink cell focus-forming murine leukemia viruses.

    abstract::The Friend or Moloney mink cell focus-forming (MCF) virus encodes a recombinant-type envelope glycoprotein, gp70, that is closely related to the membrane glycoprotein, gp55, of Friend spleen focus-forming virus (SFFV). We have shown previously that gp55 has the ability to activate cell growth by binding to the cellula...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.5.2408-2414.1991

    authors: Li JP,Baltimore D

    更新日期:1991-05-01 00:00:00

  • Chimeric influenza virus hemagglutinin proteins containing large domains of the Bacillus anthracis protective antigen: protein characterization, incorporation into infectious influenza viruses, and antigenicity.

    abstract::Large polypeptides of the Bacillus anthracis protective antigen (PA) were inserted into an influenza A virus hemagglutinin glycoprotein (HA), and the chimeric proteins were functionally characterized and incorporated into infectious influenza viruses. PA domain 1', the region responsible for binding to the other toxin...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.79.15.10003-10012.2005

    authors: Li ZN,Mueller SN,Ye L,Bu Z,Yang C,Ahmed R,Steinhauer DA

    更新日期:2005-08-01 00:00:00

  • The Epstein-Barr virus LMP1 cytoplasmic carboxy terminus is essential for B-lymphocyte transformation; fibroblast cocultivation complements a critical function within the terminal 155 residues.

    abstract::Recombinant Epstein-Barr viruses (EBVs) were made with mutated latent membrane protein 1 (LMP1) genes that express only the LMP1 amino-terminal cytoplasmic and six transmembrane domains (MS187) or these domains and the first 44 amino acids of the 200-residue LMP1 carboxy-terminal domain (MS231). After infection of pri...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.2.675-683.1995

    authors: Kaye KM,Izumi KM,Mosialos G,Kieff E

    更新日期:1995-02-01 00:00:00

  • In vitro translation products specified by the transforming region of adenovirus type 2.

    abstract::Region 1 DNA sequences (map positions 0 to 11% on the linear adenovirus 2 genome) are expressed both early and late in lytic infection and are required for transformation by the virus. During productive infection six distinct cytoplasmic RNAs are synthesized from this region. These RNAs comprise two families, each con...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.31.3.621-629.1979

    authors: Halbert DN,Spector DJ,Raskas HJ

    更新日期:1979-09-01 00:00:00

  • Origin of the vaccinia virus hemagglutinin.

    abstract::The relationship between the vaccinia virus hemagglutinin and hemadsorption was examined. Hemagglutinin synthesis was temporally related to the appearance of the hemadsorption reaction. Only chicken erythrocytes, which reacted with hemagglutinin, hemadsorbed to infected cells, and both of these reactions were inhibite...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.9.2.290-296.1972

    authors: Blackman KE,Bubel HC

    更新日期:1972-02-01 00:00:00

  • Analysis of natural variants of the human immunodeficiency virus type 1 gag-pol frameshift stem-loop structure.

    abstract::Human immunodeficiency virus type 1 uses ribosomal frameshifting for translation of the Gag-Pol polyprotein. Frameshift activities are thought to be tightly regulated. Analysis of gag p1 sequences from 270 plasma virions identified in 64% of the samples the occurrence of polymorphism that could lead to changes in ther...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.76.15.7868-7873.2002

    authors: Telenti A,Martinez R,Munoz M,Bleiber G,Greub G,Sanglard D,Peters S

    更新日期:2002-08-01 00:00:00

  • Morphological comparison of Machupo with lymphocytic choriomeningitis virus: basis for a new taxonomic group.

    abstract::Striking morphologic similarities between Machupo, Tacaribe, and lymphocytic choriomeningitis viruses were found by thin-section electron microscopy. It is proposed that these viruses be brought together into a single taxonomic group. ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.4.4.535-541.1969

    authors: Murphy FA,Webb PA,Johnson KM,Whitfield SG

    更新日期:1969-10-01 00:00:00

  • Interferon-dependent immunity is essential for resistance to primary dengue virus infection in mice, whereas T- and B-cell-dependent immunity are less critical.

    abstract::Dengue virus (DEN) causes dengue fever and dengue hemorrhagic fever/dengue shock syndrome, which are major public health problems worldwide. The immune factors that control DEN infection or contribute to severe disease are neither well understood nor easy to examine in humans. In this study, we used wild-type and cong...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/jvi.78.6.2701-2710.2004

    authors: Shresta S,Kyle JL,Snider HM,Basavapatna M,Beatty PR,Harris E

    更新日期:2004-03-01 00:00:00

  • Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.

    abstract::The herpes simplex virus type 1 (HSV-1) immediate-early protein ICP27 is an essential regulatory protein that localizes to the nuclei of infected cells. The strong nuclear localization signal (NLS) of ICP27 was identified recently and shown to reside in the amino-terminal portion of the polypeptide from residues 110 t...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.69.8.4656-4667.1995

    authors: Hibbard MK,Sandri-Goldin RM

    更新日期:1995-08-01 00:00:00

  • Nonpathogenesis of simian immunodeficiency virus infection is associated with reduced inflammation and recruitment of plasmacytoid dendritic cells to lymph nodes, not to lack of an interferon type I response, during the acute phase.

    abstract::Divergent Toll-like receptor 7 (TLR7) and TLR9 signaling has been proposed to distinguish pathogenic from nonpathogenic simian immunodeficiency virus infection in primate models. We demonstrate here that increased expression of type I interferon in pathogenic rhesus macaques compared to nonpathogenic African green mon...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01496-09

    authors: Campillo-Gimenez L,Laforge M,Fay M,Brussel A,Cumont MC,Monceaux V,Diop O,Lévy Y,Hurtrel B,Zaunders J,Corbeil J,Elbim C,Estaquier J

    更新日期:2010-02-01 00:00:00

  • A domain of the hepadnavirus capsid protein is specifically required for DNA maturation and virus assembly.

    abstract::Mutations introduced into the capsid gene of duck hepatitis B virus (DHBV) were tested for their effects on viral DNA synthesis and assembly of enveloped viruses. Four classes of mutant phenotypes were observed among a series of deletions of covering the 3' end of the capsid open reading frame. Class I mutant capsids ...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.65.5.2511-2517.1991

    authors: Yu M,Summers J

    更新日期:1991-05-01 00:00:00

  • Crystal structure of porcine reproductive and respiratory syndrome virus leader protease Nsp1alpha.

    abstract::Porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV), a positive-strand RNA virus that belongs to the Arteriviridae family of Nidovirales, has been identified as the causative agent of PRRS. Nsp1alpha is the amino (N)-terminal protein in a polyprotein encoded by the PRRSV genome and is reported to be cru...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.02579-08

    authors: Sun Y,Xue F,Guo Y,Ma M,Hao N,Zhang XC,Lou Z,Li X,Rao Z

    更新日期:2009-11-01 00:00:00

  • Tumor immunity against a simian virus 40 oncoprotein requires CD8+ T lymphocytes in the effector immune phase.

    abstract::The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...

    journal_title:Journal of virology

    pub_type: 杂志文章

    doi:10.1128/JVI.01512-09

    authors: Lowe DB,Shearer MH,Jumper CA,Bright RK,Kennedy RC

    更新日期:2010-01-01 00:00:00