Abstract:
:We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse cells were first exposed to calcium phosphate-precipitated viral DNA and then treated with dimethyl sulfoxide. Infectivity was monitored by focus formation for Ha-SV and XC plaque formation for Mo-MuLV. The viral DNA titration pattern followed single-hit kinetics for both foci and plaques, indicating that a single molecule carried information for each function. Focus-forming and plaque-forming activity were present in different molecules, since these two biological activities could be separated from each other by agarose gel electrophoresis. The focus-forming molecule was linear DNA with a molecular weight of about 4 x 10(6) daltons. The focus-forming activity of the viral DNA was sensitive to EcoRI and resistant to XhoI restriction endonucleases, whereas the plaque-forming activity was resistant to EcoRI and sensitive to XhoI. The generation of helper-independent foci indicates that Ha-SV DNA can transform mouse cells in the absence of helper virus or its proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Lowy DR,Rands E,Scolnick EMdoi
10.1128/JVI.26.2.291-298.1978subject
Has Abstractpub_date
1978-05-01 00:00:00pages
291-8issue
2eissn
0022-538Xissn
1098-5514journal_volume
26pub_type
杂志文章abstract::In coliphage 186, 22 essential genes were defined by complementation studies with amber mutants. Eighteen genes were associated with phage morphogenesis: 11 with phage tail formation, and 7 with phage head formation. The remaining four genes are discussed in the accompanying paper (S. M. Hocking and J. B. Egan, J. Vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.44.3.1056-1067.1982
更新日期:1982-12-01 00:00:00
abstract::In some retroviruses, such as Rous sarcoma virus and prototype foamy virus, Gag proteins are known to shuttle between the nucleus and the cytoplasm and are implicated in nuclear export of the viral genomic unspliced RNA (gRNA) for subsequent encapsidation. A similar function has been proposed for human immunodeficienc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06874-11
更新日期:2012-03-01 00:00:00
abstract::The coding capacity of human cytomegalovirus (HCMV) for glycoproteins by far exceeds that of other herpesviruses. Few of these proteins have been characterized so far. We have investigated the gene product of reading frame UL132. The putative protein product of UL132 is a glycoprotein with a theoretical mass of 29.8 k...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.11837-11847.2005
更新日期:2005-09-01 00:00:00
abstract::Previous studies have suggested that coxsackievirus B (CVB) activates CD8(+) T cells in vivo, but the extent of this activation and the antigen specificity of the CD8(+) T cells remain uncertain. Furthermore, CVB-induced CD4(+) T-cell responses have not been carefully investigated. Herein, we evaluate CD8(+) and CD4(+...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02639-07
更新日期:2008-05-01 00:00:00
abstract::Given the current difficulties generating vaccine-induced neutralizing antibodies to human immunodeficiency virus (HIV), the focus of the vaccine community has shifted toward creating cytotoxic-T-lymphocyte (CTL)-based vaccines. Recent reports of CTL-based vaccine trials in macaques challenged with simian/human immuno...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.24.13348-13360.2003
更新日期:2003-12-01 00:00:00
abstract::Herpesviruses utilize different origins of replication during lytic versus latent infection. Latent DNA replication depends on host cellular DNA replication machinery, whereas lytic cycle DNA replication requires virally encoded replication proteins. In lytic DNA replication, the lytic origin (ori-Lyt) is bound by a v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.10.5578-5588.2003
更新日期:2003-05-01 00:00:00
abstract::The host cell-specific factor 1 gene (hcf-1) of the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) is required for the efficient replication and/or stability of reporter plasmids carrying an AcMNPV-derived origin of DNA replication in a cell-specific manner; hcf-1 is required for reporter plasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.8.5123-5130.1996
更新日期:1996-08-01 00:00:00
abstract::The mechanism of action of hepatitis B virus (HBV) X protein in transcriptional transactivation and in tumorigenesis remains obscure. We have used the yeast two-hybrid system to identify a cellular protein that can interact with HBV X protein. This protein, designated X-associated protein 1 (XAP-1), is a human homolog...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.2.1107-1114.1995
更新日期:1995-02-01 00:00:00
abstract::Bacteriophage with linear, double-stranded DNA genomes package DNA into preassembled protein shells called procapsids. Located at one vertex in the procapsid is a portal complex composed of a ring of 12 subunits of portal protein. The portal complex serves as a docking site for the DNA packaging enzymes, a conduit for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.20.10245-10255.2002
更新日期:2002-10-01 00:00:00
abstract::Infection of newborn Lewis rats with Borna disease virus (neonatal Borna disease [NBD]) results in cerebellar damage without the cellular inflammation associated with infections in later life. Purkinje cell (PC) damage has been reported for several models of early-life viral infection, including NBD; however, the time...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02245-06
更新日期:2007-03-01 00:00:00
abstract::To gain insight into the cellular regulation of bovine leukemia virus (BLV) trans activation, a lambda-gt11 cDNA library was constructed with mRNA isolated from a BLV-induced tumor and the recombinant proteins were screened with an oligonucleotide corresponding to the tax activation-responsive element (TAR). Two clone...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.2.766-772.1992
更新日期:1992-02-01 00:00:00
abstract::The relationship between virus-specific CD8(+)-T-cell responses and viral persistence was studied in mice by using Hantaan virus (HTNV). We first established a simple method for measuring levels of virus-specific CD8(+) T cells by flow cytometry. Next, to produce a mouse model of persistent HTNV infection, newborn mic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.15.8408-8417.2003
更新日期:2003-08-01 00:00:00
abstract::Two herpes simplex virus type 1 glycoproteins, gE and gI, have been shown to form a complex that binds the Fc domain of immunoglobulin G (IgG). We demonstrate that this complex is required for the binding of monomeric nonimmune IgG but that gE alone is sufficient for binding polymeric IgG in the form of IgG complexes....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2725-2731.1990
更新日期:1990-06-01 00:00:00
abstract::The L5R gene of vaccinia virus is conserved among all sequenced members of the Poxviridae but has no predicted function or recognized nonpoxvirus homolog. Here we provide the initial characterization of the L5 protein. L5 is expressed following DNA replication with kinetics typical of a viral late protein, contains a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.17.10988-10998.2005
更新日期:2005-09-01 00:00:00
abstract::The nonsegmented negative-strand RNA viruses (order Mononegavirales) include many important human pathogens. The order of their genes, which is highly conserved, is the major determinant of the relative levels of gene expression, since genes that are close to the single promoter site at the 3' end of the viral genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.6.4705-4712.1999
更新日期:1999-06-01 00:00:00
abstract::A retrovirus endogenous to guinea pig cells was earlier shown to be morphologically similar to type B and type D prototype retroviruses. Molecular hybridization techniques were used to show that guinea pig virus nucleotide sequences are endogenous to both domestic (Cavia porcellus) and indigenous (Cavia aperea) guinea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.1.522-530.1980
更新日期:1980-01-01 00:00:00
abstract::Superinfection exclusion is the ability of an established virus infection to interfere with infection by a second virus. In this study, we found that Huh-7.5 cells acutely infected with hepatitis C virus (HCV) genotype 2a (chimeric strain J6/JFH) and cells harboring HCV genotype 1a, 1b, or 2a full-length or subgenomic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01748-06
更新日期:2007-04-01 00:00:00
abstract::Competitive quantitative PCR and reverse transcriptase-PCR were used to quantitate DNA and RNA from an attenuated ribonucleotide reductase-deleted herpes simplex virus type 1 (HSV-1) mutant in the rat trigeminal ganglion after peripheral inoculation following corneal scarification. Amplification of ganglionic DNA with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7083-7091.1994
更新日期:1994-11-01 00:00:00
abstract::Hepatitis delta virus (HDV) replicates its genome in the nucleus of an infected cell. However, an unsolved problem has been the identification in the cytoplasm of a putative mRNA for the synthesis of the only virus-coded protein, the delta antigen. We now report the characterization of an 800-base RNA that is cytoplas...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.7.3192-3198.1990
更新日期:1990-07-01 00:00:00
abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
abstract::Cytoplasmic virus-specific RNA and polyribosomes from a chronically infected feline thymus tumor cell line, F-422, were analyzed by using in vitro-synthesized feline leukemia virus (Rickard strain) (R-FeLV) complementary DNA (cDNA) probe. By hybridization kinetics analysis, cytoplasmic, polyribosomat, and nuclear RNAs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.3.750-763.1978
更新日期:1978-03-01 00:00:00
abstract::We previously reported that the expression of rotavirus phenotypes by reassortants was affected by recipient genetic background and proposed specific interactions between the outer capsid proteins VP4 and VP7 as the basis for the phenotypic effects (D. Chen, J. W. Burns, M. K. Estes, and R. F. Ramig, Proc. Natl. Acad....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.1.432-439.1992
更新日期:1992-01-01 00:00:00
abstract:UNLABELLED:Aspects of intrinsic antiviral immunity are mediated by promyelocytic leukemia nuclear body (PML-NB) constituent proteins. During herpesvirus infection, these antiviral proteins are independently recruited to nuclear domains that contain infecting viral genomes to cooperatively promote viral genome silencing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00426-16
更新日期:2016-06-10 00:00:00
abstract::The deoxyribonucleic acid (DNA) of Escherichia coli B is converted by colicin E2 to products soluble in cold trichloroacetic acid; we show that this DNA degradation (hereafter termed solubilization) is subject to inhibition by infection with bacteriophage T4. At least two modes of inhibition may be differentiated on t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.3.303-310.1971
更新日期:1971-09-01 00:00:00
abstract::An in vitro assay was developed to investigate endonuclease activity of Thogoto virus, a tick-borne orthomyxovirus. Endonuclease activity relied on an interaction between the 3' and 5' termini of virion RNA (vRNA) and not those of cRNA. Evidence was obtained that cap structures are cleaved directly from cap donors and...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.2305-2309.1998
更新日期:1998-03-01 00:00:00
abstract::Adenovirus (Ad) gene transfer vectors can be used to transfer and express antigens and function as strong adjuvants and thus are useful platforms for the development of genetic vaccines. Based on the hypothesis that Ad vectors with enhanced infectibility of dendritic cells (DC) may be able to evoke enhanced immune res...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.5.2572-2580.2004
更新日期:2004-03-01 00:00:00
abstract::To investigate the process of human immunodeficiency virus type 1 (HIV-1) evolution in vivo, a total of 179 HIV-1 V3 sequences derived from cell-free plasma were determined from serial samples in three epidemiologically linked individuals (one infected blood donor and two transfusion recipients) over a maximum period ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.3.2555-2561.1997
更新日期:1997-03-01 00:00:00
abstract::Temperature-sensitive (ts) mutants of the T4 phage rII gene were islated and used in temperature shift experiments that revelaed two different expressions for the normal rII (rII+) gene function in vivo: (i) an early expression (0 to 12 min postinfection at 30 C) that prevents restriction of T4 growth in Escherichia c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.974-981.1975
更新日期:1975-10-01 00:00:00
abstract::Recently it has been shown that kaposica, an immune evasion protein of Kaposi's sarcoma-associated herpesvirus, inactivates complement by acting on C3-convertases by accelerating their decay as well as by acting as a cofactor in factor I-mediated inactivation of their subunits C3b and C4b. Here, we have mapped the fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5850-5856.2005
更新日期:2005-05-01 00:00:00
abstract::Current adeno-associated virus (AAV) gene therapy vectors package a transgene flanked by the terminal repeats (TRs) of AAV type 2 (AAV2). Although these vectors are replication deficient, wild-type (wt) AAV2 prevalent in the human population could lead to replication and packaging of a type 2 TR (TR2)-flanked transgen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02466-08
更新日期:2009-04-01 00:00:00