Abstract:
:The relationship between virus-specific CD8(+)-T-cell responses and viral persistence was studied in mice by using Hantaan virus (HTNV). We first established a simple method for measuring levels of virus-specific CD8(+) T cells by flow cytometry. Next, to produce a mouse model of persistent HTNV infection, newborn mice were inoculated subcutaneously within 24 h of birth with 1 or 0.1 50% newborn mouse lethal dose of HTNV. All mice that escaped lethal infection were persistently infected with HTNV until at least 30 days after virus inoculation and had no virus-specific CD8(+) T cells producing gamma interferon (IFN-gamma). Subsequently, the virus was eliminated from some of the mice, depending on the appearance of functional virus-specific CD8(+) T cells, which have the ability to produce IFN-gamma and tumor necrosis factor alpha (TNF-alpha) and have cytotoxic activity. Neutralizing antibodies were detected in all mice, regardless of the presence or absence of virus. In the acute phase, which occurs within 30 days of infection, IFN-gamma-producing HTNV-specific CD8(+) T cells were detected on day 15 after virus inoculation. However, TNF-alpha production and the cytotoxic activity of these specific CD8(+) T cells were impaired and HTNV was not removed. Almost all of these specific CD8(+) T cells disappeared by day 18. These results suggest that functional HTNV-specific CD8(+) T cells are important for clearance of HTNV.
journal_name
J Viroljournal_title
Journal of virologyauthors
Araki K,Yoshimatsu K,Lee BH,Kariwa H,Takashima I,Arikawa Jdoi
10.1128/jvi.77.15.8408-8417.2003subject
Has Abstractpub_date
2003-08-01 00:00:00pages
8408-17issue
15eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
杂志文章abstract:UNLABELLED:Recent evidence suggests that even in treated infections, human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication may continue in lymph nodes (LN), serving as a potential virus reservoir. Here we investigated the effects of lentivirus infection on natural killer (NK) cell frequ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00660-15
更新日期:2015-07-01 00:00:00
abstract::Two strains of minute virus of mice (MVM) show different host cell specificities. The prototype strain MVM(p) grows in fibroblasts, whereas the immunosuppressive variant MVM(i) grows in T lymphocytes. In this study, we have mapped on the viral genome a cell type-specific determinant: it is located between 69 and 85 ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.2.552-557.1988
更新日期:1988-02-01 00:00:00
abstract::Nectin-1 and nectin-2 are related molecules that can function with different specificities as entry receptors for mammalian alphaherpesviruses through interaction with viral glycoprotein D (gD). The normal function of members of the nectin family is to mediate cell-cell adhesion through homotypic and heterotypic necti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12940-12950.2002
更新日期:2002-12-01 00:00:00
abstract::Neurotropic coronavirus induces acute encephalomyelitis and demyelination in mice. Infection of BALB/c (H-2(d)) mice expressing a dominant negative gamma interferon (IFN-gamma) receptor specifically in oligodendrocytes was examined to determine the influence of IFN-gamma signaling on pathogenesis. Inhibition of IFN-ga...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02373-09
更新日期:2010-03-01 00:00:00
abstract::Proteolytic processing of the picornaviral polyprotein mediated by the differential action of virus-encoded proteinase(s) is pivotal to both RNA genome replication and capsid formation. Possibly to enlarge the array of viral proteins, picornaviral polyprotein processing results in intermediate and mature products whic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.10.8013-8020.1998
更新日期:1998-10-01 00:00:00
abstract::The major human tRNA(Lys) isoacceptors, and, are selectively packaged into human immunodeficiency virus type 1 (HIV-1) during assembly, where acts as a primer for reverse transcription. Lysyl-tRNA synthetase (LysRS) is also incorporated into HIV-1, independently of tRNA(Lys), via its interaction with Gag, and is a str...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.18.9817-9822.2003
更新日期:2003-09-01 00:00:00
abstract::Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression wa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.2.1630-1636.1999
更新日期:1999-02-01 00:00:00
abstract::Endogenous viral elements derived from nonretroviral RNA viruses have been described in various animal genomes. Whether they have a biological function, such as host immune protection against related viruses, is a field of intense study. Here, we investigated the repertoire of endogenous flaviviral elements (EFVEs) in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00571-17
更新日期:2017-07-12 00:00:00
abstract::We describe a modification of the polymerase chain reaction technique which allows amplification of cellular DNA adjacent to an integrated provirus given sequence information for the provirus only. The modified technique should be generally useful for studies of insertional mutagenesis and other situations in which on...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.5.1924-1928.1989
更新日期:1989-05-01 00:00:00
abstract::Recombinant retroviruses containing the trans activator genes of human T-cell leukemia virus (HTLV) type II and human T-cell lymphotropic virus type III were constructed. The trans activator genes tat II and tat III were inserted into the murine retroviral vector pZIPNEOSV(X)1. Recombinant plasmids were transfected in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.1.379-384.1986
更新日期:1986-01-01 00:00:00
abstract::A recombinant vaccinia virus genome was constructed in which the viral thymidine kinase (tk) gene was placed between direct repeats of a 1.5-kilobase-pair DNA sequence of heterologous origin. When forced to replicate in tk- cells in the presence of methotrexate (i.e., under tk+-selective conditions), the recombinant m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.6.1788-1795.1987
更新日期:1987-06-01 00:00:00
abstract::We have investigated the antigenicity of the C- and N-terminal halves of pIX of human adenovirus types 2 and 3 (Ad2 and Ad3) as well as their orientations in virions. We found that only the C-terminal halves of Ad2 pIX and Ad3 pIX reacted in a subgenus-specific manner by enzyme-linked immunosorbent assay and immunoblo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.6182-6187.1999
更新日期:1999-07-01 00:00:00
abstract::All hepatitis B viruses replicate by protein-primed reverse transcription, employing a specialized reverse transcriptase, P protein, that carries a unique terminal protein (TP) domain. To initiate reverse transcription, P protein must bind to a stem-loop, epsilon, on the pregenomic RNA template. TP then provides a Y r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01196-07
更新日期:2007-12-01 00:00:00
abstract::The locations of replication pause sites in the simian virus 40 minichromosome which were determined by sizing cloned fragments of nascent DNA (Zannis-Hadjopoulos et al., J. Mol. Biol. 165:599-607, 1983) were compared with the positions of simian virus 40 nucleosomes in the genome, as obtained by sequence-directed map...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.3.1011-1012.1984
更新日期:1984-12-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) immediate-early protein ICP27 is an essential regulatory protein that localizes to the nuclei of infected cells. The strong nuclear localization signal (NLS) of ICP27 was identified recently and shown to reside in the amino-terminal portion of the polypeptide from residues 110 t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.8.4656-4667.1995
更新日期:1995-08-01 00:00:00
abstract::The locations and functions of DNA sequences involved in transcription of the gene encoding herpes simplex virus type 1 immediate early (IE) mRNAs 4 and 5 were analyzed by use of a transient-expression assay. The region upstream of the genes encoding IE mRNAs 4 and 5 was fused to the thymidine kinase gene coding seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.708-716.1984
更新日期:1984-06-01 00:00:00
abstract::Leukocyte access into the central nervous system (CNS) parenchyma is tightly regulated by the blood-brain barrier (BBB). Leukocyte migration through the endothelial cell wall into the perivascular space is well characterized; however, mechanisms regulating their penetration through the glia limitans into the parenchym...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00051-10
更新日期:2010-05-01 00:00:00
abstract::Massive infection of memory CD4 T cells is a hallmark of early simian immunodeficiency virus (SIV) infection, with viral infection peaking at day 10 postinfection (p.i.), when a majority of memory CD4 T cells in mucosal and peripheral tissues are infected. It is not clear if mononuclear cells from the monocyte and mac...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06372-11
更新日期:2012-01-01 00:00:00
abstract::Equine herpesvirus type 1 (EHV-1), a member of the Alphaherpesviridae, displays a broad host range in vitro, allowing for detailed study of the mechanisms of productive infection, including attachment and entry, in various cell culture systems. Previously, we showed that EHV-1 infects Chinese hamster ovary (CHO-K1) ce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00504-07
更新日期:2007-10-01 00:00:00
abstract::Vpu is a 16-kDa membrane-associated phosphoprotein that is expressed from the same, singly spliced message as the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein precursor, gp160. Previous studies suggest that Vpu functions in the late stages of viral replication, possibly in virus egression from the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.4190-4194.1993
更新日期:1993-07-01 00:00:00
abstract::Since dendritic cells may play a key role in defense against influenza virus infection, we examined the effects of recombinant hemagglutinin (HA) proteins derived from mouse-adapted H1N1 (A/WSN/1933), swine-origin 2009 pandemic H1N1 (A/Texas/05/2009), and highly pathogenic avian influenza H5N1 (A/Thailand/KAN-1/2004) ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01316-10
更新日期:2010-11-01 00:00:00
abstract::Tryptic peptide maps were prepared for four purified structural proteins derived from several murine leukemia viruses (MuLV's). Analyses of these peptide maps reveal that the p30 proteins of Rauscher, Moloney, and Gross MuLV's are very similar to each other, as are the p10's obtained from these three viruses. In contr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.15.5.1148-1157.1975
更新日期:1975-05-01 00:00:00
abstract::We showed previously that retrovirus vector particles can encapsidate RNAs without retroviral cis-acting sequences, that such RNAs are reverse transcribed in infected target cells, and that the cDNA copies are inserted into the host genome resulting in cDNA genes (R. Dornburg and H. M. Temin, Mol. Cell. Biol. 8:2328-2...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.2.886-889.1990
更新日期:1990-02-01 00:00:00
abstract::Latency-associated transcript (LAT) promoter deletion mutants of herpes simplex virus type 1 have a reduced capacity to reactivate following adrenergic induction in the rabbit eye model. We have mapped a reactivation phenotype within LAT and describe the construction of recombinants in which poly(A) addition sites hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2449-2459.1996
更新日期:1996-04-01 00:00:00
abstract::In all retroviruses analyzed to date (except for the spumaretroviruses), the Zn(2+)-coordinating residues of nucleocapsid (NC) perform or assist in crucial reactions necessary to complete the retrovirus life cycle. Six replication-defective mutations have been engineered in the two NC Zn(2+) fingers (ZFs) of simian im...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.115-124.2001
更新日期:2001-01-01 00:00:00
abstract::Influenza virus particles bind rapidly to vesicular stomatitis, Sindbis, or Rauscher murine leukemia virus particles, forming mixed aggregates demonstrable by electron microscopy. The normal hemagglutinating property of influenza virus is inhibited by these viruses, providing a rapid quantitative assay. Prior treatmen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.5.1307-1309.1974
更新日期:1974-11-01 00:00:00
abstract::A recent experiment involving simian immunodeficiency virus (SIV) infection of macaques revealed that the infectivity of this virus decreased over the first few months of infection. Based on this observation, we introduce a viral dynamic model in which viral infectivity varies over time. The model is fit to viral load...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02284-09
更新日期:2010-05-01 00:00:00
abstract::Recent studies of varicella-zoster virus (VZV) DNA sequence variation, involving large numbers of globally distributed clinical isolates, suggest that this virus has diverged into at least three distinct genotypes designated European (E), Japanese (J), and mosaic (M). In the present study, we determined and analyzed t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00835-06
更新日期:2006-10-01 00:00:00
abstract::Strains of Bacillus that harbor defective phage PBSX were found to be insensitive to SP-10(C), although the phage adsorbed to these insensitive strains. Strains that did not carry the phage were sensitive to SP-10(C). B. subtilis 168 ind(-), which can be tranduced by SP-10(C) but is nonpermissive for the phage, was re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:
更新日期:1968-08-01 00:00:00
abstract::AIDS vaccination has a pressing need for more potent vaccination vectors capable of eliciting strong, diversified, and long-lasting cellular immune responses against human immunodeficiency virus (HIV). Lentiviral vectors have demonstrated efficiency not only as gene delivery vehicles for gene therapy applications but ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01284-09
更新日期:2009-11-01 00:00:00