Abstract:
:The herpes simplex virus type 1 (HSV-1) immediate-early protein ICP27 is an essential regulatory protein that localizes to the nuclei of infected cells. The strong nuclear localization signal (NLS) of ICP27 was identified recently and shown to reside in the amino-terminal portion of the polypeptide from residues 110 to 137 (W.E. Mears, V. Lam, and S.A. Rice, J. Virol. 69:935-947, 1995). There are also two arginine-rich regions directly succeeding the NLS. The first of these arginine-rich sequences (residues 141 to 151), together with the NLS, has been shown by Mears et al. to form the nucleolar localization signal. Arginine-rich motifs are common in domains involved in nuclear localization and RNA binding. To analyze the role of the arginine-rich regions in ICP27, we constructed stably transformed cell lines containing ICP27 mutants with deletions of all or parts of the NLS and arginine-rich regions. We also constructed mutants in which these regions were replaced with heterologous NLSs or RNA-binding domains. Characterization of these mutants indicated that the arginine-rich regions were required but not sufficient for wild-type localization of ICP27. More importantly, the NLS and arginine-rich regions were also essential to the function of ICP27. Mutants lacking these sequences were defective in late gene expression during infection even when ICP27 was properly localized to the nucleus by substitution of the NLS from simian virus 40 large T antigen. Further, the defect in late gene expression could not be overcome by replacement with the highly basic RNA-binding domain of human immunodeficiency virus type 1 Tat. The deficiency in late gene expression was independent of ICP27's role in stimulating viral DNA replication. In addition, localization of the HSV-1 proteins ICP4, ICP0, and ICP8 was unaffected by ICP27 mutants in this region. These results suggest that the arginine-rich regions are required for efficient nuclear localization and for the regulatory activity of ICP27 involved in viral late gene expression.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hibbard MK,Sandri-Goldin RMdoi
10.1128/JVI.69.8.4656-4667.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
4656-67issue
8eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the lef...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.1.320-329.1998
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abstract::Smallpox was eradicated more than 10 years ago, but infection with another Orthopoxvirus, monkeypox virus, can result in a clinical picture resembling smallpox. Human infection with monkeypox virus is extremely rare, not easily transmitted, and confined to the rain forest belt of Africa (Z. Jezek and F. Fenner, p. 81-...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7565-7567.1992
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doi:10.1128/JVI.64.10.5173-5176.1990
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abstract::Equine infectious anemia virus (EIAV) provides a natural model system by which immunological control of lentivirus infections may be studied. To date, no detailed study addressing in parallel both the humoral and cellular immune responses induced in horses upon infection by EIAV has been conducted. Therefore, we initi...
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doi:10.1128/JVI.71.5.3840-3852.1997
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pub_type: 杂志文章
doi:10.1128/JVI.69.11.6859-6864.1995
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doi:10.1128/JVI.00338-07
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doi:10.1128/JVI.01679-08
更新日期:2009-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.120-128.1997
更新日期:1997-01-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) sequences were generated from blood and from brain tissue obtained by stereotactic biopsy from six patients undergoing a diagnostic neurosurgical procedure. Proviral DNA was directly amplified by nested PCR, and 8 to 36 clones from each sample were sequenced. Phylogenetic an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7467-7481.1994
更新日期:1994-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.11.5315-5325.2002
更新日期:2002-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00374-06
更新日期:2006-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02279-15
更新日期:2015-11-04 00:00:00
abstract::The V protein of Sendai virus (SeV) is nonessential to virus replication in cell culture but indispensable to viral pathogenicity in mice. The highly conserved cysteine-rich zinc finger-like domain in its carboxyl terminus is believed to be responsible for this viral pathogenicity. In the present study, we showed that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.17.7834-7841.2000
更新日期:2000-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1886-1893.1998
更新日期:1998-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.75.5.2059-2066.2001
更新日期:2001-03-01 00:00:00
abstract::The E7 proteins of human papillomaviruses (HPVs) promote S-phase reentry in differentiated keratinocytes of the squamous epithelia to support viral DNA amplification. In this study, we showed that nuclear p130 was present in the differentiated strata of several native squamous epithelia susceptible to HPV infection. I...
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pub_type: 杂志文章
doi:10.1128/JVI.64.2.886-889.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.1.548-554.1994
更新日期:1994-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.2.892-900.1989
更新日期:1989-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1782-1789.1994
更新日期:1994-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.60.1.275-285.1986
更新日期:1986-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-09-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.449-461.1982
更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3832-3840.1987
更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00