Interaction of the C-terminal domains of sendai virus N and P proteins: comparison of polymerase-nucleocapsid interactions within the paramyxovirus family.

abstract：:In the spherical capsid of hepatitis B virus (HBV), intermolecular disulfide bonds cross-link the approximately 180 p21.5 capsid protein subunits into a stable lattice. In this study, we used mutant capsid proteins to investigate the role that disulfide bonds and the four p21.5 Cys residues (positions 48, 61, 107, and...

journal_title：Journal of virology

authors： Zhou S,Standring DN

更新日期：1992-09-01 00:00:00

abstract：:Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus and one of the most common agents of viral encephalitis. The infectious entry process of JEV into host cells remains largely unknown. Here, we present a systemic study concerning the cellular entry mechanism of JEV to B104 rat neuroblastoma cells. It was...

journal_title：Journal of virology

authors： Zhu YZ,Xu QQ,Wu DG,Ren H,Zhao P,Lao WG,Wang Y,Tao QY,Qian XJ,Wei YH,Cao MM,Qi ZT

更新日期：2012-12-01 00:00:00

abstract：:Natural evolution in primate lentiviral reverse transcriptase (RT) appears to have been constrained by the necessity to maintain function within an asymmetric protein composed of two identical primary amino acid sequences (66 kDa), of which one is cleaved (51 kDa). In this study, a detailed phylogenetic analysis now s...

journal_title：Journal of virology

authors： Tebit DM,Lobritz M,Lalonde M,Immonen T,Singh K,Sarafianos S,Herchenröder O,Kräusslich HG,Arts EJ

更新日期：2010-10-01 00:00:00

abstract：UNLABELLED:Tetherin (BST2, CD317, or HM1.24) is a host cellular restriction factor that prevents the release of enveloped viruses by mechanically linking virions to the plasma membrane. The precise arrangement of tetherin molecules at the plasma membrane site of HIV-1 assembly, budding, and restriction is not well unde...

journal_title：Journal of virology

authors： Strauss JD,Hammonds JE,Yi H,Ding L,Spearman P,Wright ER

更新日期：2015-11-18 00:00:00

abstract：:Physiological microenvironments such as blood, seminal plasma, mucosal secretions, or lymphatic fluids may influence the biology of the virus-host cell and immune interactions for human immunodeficiency virus type 1 (HIV-1). Relative to media, physiological levels of human plasma were found to enhance the infectivity ...

journal_title：Journal of virology

authors： Wu SC,Spouge JL,Conley SR,Tsai WP,Merges MJ,Nara PL

更新日期：1995-10-01 00:00:00

abstract：UNLABELLED:Influenza virus transcription requires functional coupling with cellular transcription for the cap-snatching process. Despite this fact, RNA polymerase II (RNAP II) is degraded during infection in a process triggered by the viral polymerase. Reassortant viruses from the A/PR/8/34 (PR8) strain that induce (hv...

journal_title：Journal of virology

authors： Llompart CM,Nieto A,Rodriguez-Frandsen A

更新日期：2014-03-01 00:00:00

abstract：:The mechanism by which bovine herpesvirus 1 (BHV-1) predisposes cattle to bacterial pneumonia was investigated by using an in vitro system to demonstrate immunosuppression. At a multiplicity of infection of 0.001, live or inactivated BHV-1 induced a 50% inhibition of the proliferative response of peripheral blood mono...

journal_title：Journal of virology

authors： Hutchings DL,Campos M,Qualtiere L,Babiuk LA

更新日期：1990-09-01 00:00:00

abstract：UNLABELLED:Numerous experimental studies have demonstrated that CD8(+) T cells contribute to immunity against influenza by limiting viral replication. It is therefore surprising that rigorous statistical tests have failed to find evidence of positive selection in the epitopes targeted by CD8(+) T cells. Here we use a n...

journal_title：Journal of virology

authors： Machkovech HM,Bedford T,Suchard MA,Bloom JD

更新日期：2015-11-01 00:00:00

abstract：:S-Adenosylhomocysteine was found to have no effect on Epstein-Barr virus-induced transformation of B-lymphocytes and to stimulate viral capsid antigen expression only slightly in the FF41-1 cell line. In contrast, the S-adenosylhomocysteine analogs sinefungin and S-isobutyladenosine inhibited Epstein-Barr virus transf...

journal_title：Journal of virology

authors： Long WK,Fronko GE,Lindemeyer RG,Wu B,Henderson EE

更新日期：1987-01-01 00:00:00

abstract：UNLABELLED:To investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) tegument protein UL51 promotes viral replication, we screened for viral proteins that interact with UL51 in infected cells. Affinity purification of tagged UL51 in HSV-1-infected Vero cells was coupled with immunoblotting of th...

journal_title：Journal of virology

authors： Oda S,Arii J,Koyanagi N,Kato A,Kawaguchi Y

更新日期：2016-09-12 00:00:00

abstract：:When human A431 cells, which carry high numbers of epidermal growth factor (EGF) receptors, are exposed to EGF, the total content of phosphotyrosine in cell protein is increased, the EGF receptor becomes phosphorylated at tyrosine, and new phosphotyrosine-containing 36,000- and 81,000-dalton proteins are detected. We ...

journal_title：Journal of virology

authors： Cooper JA,Scolnick EM,Ozanne B,Hunter T

更新日期：1983-12-01 00:00:00

abstract：:Complement, part of the innate immune system, acts to remove pathogens and unwanted host material. Complement is known to function in all tissues, including the central nervous system (CNS). In this study, we demonstrated the importance of the complement system within the CNS in the development of behavioral seizures ...

journal_title：Journal of virology

authors： Libbey JE,Kirkman NJ,Wilcox KS,White HS,Fujinami RS

更新日期：2010-07-01 00:00:00

abstract：:A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have...

journal_title：Journal of virology

authors： Jainchill JL,Aaronson SA,Todaro GJ

更新日期：1969-11-01 00:00:00

abstract：:DNA sequences encoding the surface envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) were amplified by PCR from uncultured peripheral blood mononuclear cells obtained from patients with serologically defined HIV-1/HIV-2 mixed infections from Bombay, India. HIV-1-specific PCR prod...

journal_title：Journal of virology

authors： Grez M,Dietrich U,Balfe P,von Briesen H,Maniar JK,Mahambre G,Delwart EL,Mullins JI,Rübsamen-Waigmann H

更新日期：1994-04-01 00:00:00

abstract：:The temperature-sensitive and attenuated phenotypes of the Sabin type 1 vaccine strain of poliovirus result from numerous point mutations which occurred in the virulent Mahoney virus parent. One of these mutations is located in a 3D polymerase (3Dpol) codon (U-6203-->C, Tyr-73-->His) and is involved in attenuation in ...

journal_title：Journal of virology

authors： Georgescu MM,Tardy-Panit M,Guillot S,Crainic R,Delpeyroux F

更新日期：1995-09-01 00:00:00

abstract：:By pretreating simian virus 40-infected BSC-1 cells with glucosamine, [(3)H]uridine labeling of both cellular and viral RNA can be halted instantaneously by addition of cold uridine. We have studied the fate of pulse-labeled viral RNA from cells at 45 h postinfection under these conditions. During a 5-min period of la...

journal_title：Journal of virology

authors： Chiu NH,Radonovich MF,Thoren MM,Salzman NP

更新日期：1978-11-01 00:00:00

abstract：:Marburg virus, a filovirus, causes severe hemorrhagic fever with hitherto poorly understood molecular pathogenesis. We have investigated here the vectorial transport of the surface protein GP of Marburg virus in polarized epithelial cells. To this end, we established an MDCKII cell line that was able to express GP per...

journal_title：Journal of virology

authors： Sänger C,Mühlberger E,Ryabchikova E,Kolesnikova L,Klenk HD,Becker S

更新日期：2001-02-01 00:00:00

abstract：:We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5delta32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral bl...

journal_title：Journal of virology

authors： Gray L,Churchill MJ,Keane N,Sterjovski J,Ellett AM,Purcell DF,Poumbourios P,Kol C,Wang B,Saksena NK,Wesselingh SL,Price P,French M,Gabuzda D,Gorry PR

更新日期：2006-04-01 00:00:00

abstract：:Reduction of major histocompatibility complex class I cell surface expression occurs in adenovirus-, herpes simplex virus-, human cytomegalovirus (HCMV)-, and murine cytomegalovirus-infected cell systems. Recently, it was demonstrated that the down-regulation mediated by HCMV infection is posttranslational, as a resul...

journal_title：Journal of virology

authors： Jones TR,Hanson LK,Sun L,Slater JS,Stenberg RM,Campbell AE

更新日期：1995-08-01 00:00:00

abstract：:Swine influenza viruses (SIV) naturally infect pigs and can be transmitted to humans. In the pig, genetic reassortment to create novel influenza subtypes by mixing avian, human, and swine influenza viruses is possible. An SIV vaccine inducing cross-protective immunity between different subtypes and strains circulating...

journal_title：Journal of virology

authors： Richt JA,Lekcharoensuk P,Lager KM,Vincent AL,Loiacono CM,Janke BH,Wu WH,Yoon KJ,Webby RJ,Solórzano A,García-Sastre A

更新日期：2006-11-01 00:00:00

abstract：:Stress signaling from mitochondria and the endoplasmic reticulum (ER) leads to the induction of the proapoptotic transcription factor CHOP/GADD153. Many viruses use the ER as a site of replication and/or envelopment, and this activity can lead to the activation of ER stress and apoptosis. African swine fever virus (AS...

journal_title：Journal of virology

authors： Netherton CL,Parsley JC,Wileman T

更新日期：2004-10-01 00:00:00

abstract：:Several lines of evidence suggest that various cofactors may be required for prion replication. PrP binds to polyanions, and RNAs were shown to promote the conversion of PrP(C) into PrP(Sc) in vitro. In the present study, we investigated strain-specific differences in RNA requirement during in vitro conversion and the...

journal_title：Journal of virology

authors： Saá P,Sferrazza GF,Ottenberg G,Oelschlegel AM,Dorsey K,Lasmézas CI

更新日期：2012-10-01 00:00:00

abstract：:The mammalian APOBEC3 proteins are an important component of the cellular innate immune response to retroviral infection. APOBEC3G can extinguish HIV-1 infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity that attacks the nascent viral cDNA during reverse transcription, caus...

journal_title：Journal of virology

authors： Sadler HA,Stenglein MD,Harris RS,Mansky LM

更新日期：2010-07-01 00:00:00

abstract：:To assess the RNA helicase activity of hepatitis C virus (HCV) nonstructural protein 3 (NS3), a polypeptide encompassing amino acids 1175 to 1657, which cover only the putative helicase domain, was expressed in Escherichia coli by a pET expression vector. The protein was purified to near homogeneity and assayed for RN...

journal_title：Journal of virology

authors： Tai CL,Chi WK,Chen DS,Hwang LH

更新日期：1996-12-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) primary infection is characterized by the use of CCR5 as a coreceptor for viral entry, which is associated with the non-syncytium-inducing (NSI) phenotype in lymphoid cells. Syncytium-inducing (SI) variants of HIV-1 appear in advanced stages of HIV-1 infection and are charac...

journal_title：Journal of virology

authors： Llano A,Barretina J,Gutiérrez A,Blanco J,Cabrera C,Clotet B,Esté JA

更新日期：2001-11-01 00:00:00

abstract：:Recently, novel arenaviruses were found in snakes with boid inclusion body disease (BIBD); these form the new genus Reptarenavirus within the family Arenaviridae. We used next-generation sequencing and de novo sequence assembly to investigate reptarenavirus isolates from our previous study. Four of the six isolates an...

journal_title：Journal of virology

authors： Hepojoki J,Salmenperä P,Sironen T,Hetzel U,Korzyukov Y,Kipar A,Vapalahti O

更新日期：2015-08-01 00:00:00

abstract：:Marek's disease virus (MDV) is a herpesvirus of chickens that induces T lymphomas and tumors within 4 to 5 weeks of infection. Although the ability of MDV to induce tumors was demonstrated many years ago and although a number of viral oncogenic proteins have been identified, the mechanism by which the MDV is implicate...

journal_title：Journal of virology

authors： Fragnet L,Blasco MA,Klapper W,Rasschaert D

更新日期：2003-05-01 00:00:00

abstract：:Recent studies suggest the existence of cancer stem cells (CSC) and cancer progenitor cells (CPC), although strict definitions of neither CSC nor CPC have been developed. We have produced evidence that the principal oncoprotein of Epstein-Barr virus (EBV), latent membrane protein 1 (LMP1), which is associated with hum...

journal_title：Journal of virology

authors： Kondo S,Wakisaka N,Muramatsu M,Zen Y,Endo K,Murono S,Sugimoto H,Yamaoka S,Pagano JS,Yoshizaki T

更新日期：2011-11-01 00:00:00

abstract：:Synthetic peptides of increasing length and corresponding in sequence to the C-terminal end of the HA1 molecule of influenza virus were constructed and examined for their immunogenic and antigenic properties. Peptides containing at least the four C-terminal amino acids, when coupled to keyhole limpet hemocyanin, were ...

journal_title：Journal of virology

authors： Tang XL,Tregear GW,White DO,Jackson DC

更新日期：1988-12-01 00:00:00

abstract：:The major immediate-early (MIE) gene of human cytomegalovirus (HCMV) produces multiple mRNAs through differential splicing and polyadenylation. Reverse transcriptase PCR was used to characterize transcripts from exons 1, 2, 3, and 4 (immediate-early 1 [IE1]). The expected IE72 and IE19 mRNAs were detected, as well as ...

journal_title：Journal of virology

authors： Awasthi S,Isler JA,Alwine JC

更新日期：2004-08-01 00:00:00