Mapping of mutations contributing to the temperature sensitivity of the Sabin 1 vaccine strain of poliovirus.


:The temperature-sensitive and attenuated phenotypes of the Sabin type 1 vaccine strain of poliovirus result from numerous point mutations which occurred in the virulent Mahoney virus parent. One of these mutations is located in a 3D polymerase (3Dpol) codon (U-6203-->C, Tyr-73-->His) and is involved in attenuation in common mice (M. Tardy-Panit, B. Blondel, A. Martin, F. Tekaia, F. Horaud, and F. Delpeyroux, J. Virol. 67:4630-4638, 1993). This mutation also appears to contribute to temperature sensitivity, in association with at least 1 other of the 10 mutations of the 3'-terminal part of the genome including the 3Dpol coding and 3' noncoding regions. To map the other mutation(s), we constructed poliovirus mutants by mutagenesis and recombination of Mahoney and Sabin 1 cDNAs. Characterization of these poliovirus mutants showed that a second mutation in a 3Dpol codon (C-7071-->U, Thr-362-->Ile) contributes to temperature sensitivity. A mutation in the 3' noncoding region of the genome (A-7441-->G), alone or linked to another mutation (U-7410-->C), also appeared to be involved in this phenotype. The temperature-sensitive effect associated with the 3'-terminal part of the Sabin 1 genome results from the cumulative and/or synergistic effects of at least three genetic determinants, i.e., the His-73 and Ile-362 codons of 3Dpol and nucleotide G-7441. Sequence analysis of strains isolated from patients with vaccine-associated paralytic poliomyelitis showed that these genetic determinants are selected against in vivo, although the Ile-362 codon appeared to be more stable than either the His-73 codon or G-7441. These genetic determinants may contribute to the safety of Sabin 1 in vaccines.


J Virol


Journal of virology


Georgescu MM,Tardy-Panit M,Guillot S,Crainic R,Delpeyroux F




Has Abstract


1995-09-01 00:00:00












  • Role for the αV Integrin Subunit in Varicella-Zoster Virus-Mediated Fusion and Infection.

    abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yang E,Arvin AM,Oliver SL

    更新日期:2016-07-27 00:00:00

  • Mapping of betapapillomavirus human papillomavirus 5 transcription and characterization of viral-genome replication function.

    abstract::Betapapillomavirus replication and transcription have not been studied in detail because of a lack of suitable cellular systems supporting human papillomavirus (HPV) genome replication. We have recently shown that the human osteosarcoma cell line U2OS provides a useful environment for the genome replication of many di...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Sankovski E,Männik A,Geimanen J,Ustav E,Ustav M

    更新日期:2014-01-01 00:00:00

  • Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted.

    abstract::A mutant of herpes simplex virus type 1 (HSV-1) in which glycoprotein H (gH) coding sequences were deleted and replaced by the Escherichia coli lacZ gene under the control of the human cytomegalovirus IE-1 gene promoter was constructed. The mutant was propagated in Vero cells which contained multiple copies of the HSV...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Forrester A,Farrell H,Wilkinson G,Kaye J,Davis-Poynter N,Minson T

    更新日期:1992-01-01 00:00:00

  • The immunogenic glycoprotein gp35-37 of human herpesvirus 8 is encoded by open reading frame K8.1.

    abstract::Human herpesvirus 8 (HHV-8) is likely to be involved in the pathogenesis of Kaposi's sarcoma (KS) and body cavity-based lymphomas (BCBLs). The HHV-8 genome is primarily in a latent state in BCBL-derived cell lines like BCBL-1, but lytic replication can be induced by phorbol esters (R. Renne, W. Zhang, B. Herndier, M. ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Raab MS,Albrecht JC,Birkmann A,Yağuboğlu S,Lang D,Fleckenstein B,Neipel F

    更新日期:1998-08-01 00:00:00

  • Conformational Changes in the 5' End of the HIV-1 Genome Dependent on the Debranching Enzyme DBR1 during Early Stages of Infection.

    abstract::Previous studies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early steps in HIV cDNA formation but is necessary for synthesis of intermediate and late cDNA products. To further characterize this effect, we evaluated the topology of the 5' end of the HIV-1 RNA genome during early...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Galvis AE,Fisher HE,Fan H,Camerini D

    更新日期:2017-11-14 00:00:00

  • Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination.

    abstract::RNA recombination is a major process in promoting rapid virus evolution in an infected host. A previous genome-wide screen with the yeast single-gene deletion library of 4,848 strains, representing approximately 80% of all genes of yeast, led to the identification of 11 host genes affecting RNA recombination in Tomato...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Serviene E,Jiang Y,Cheng CP,Baker J,Nagy PD

    更新日期:2006-02-01 00:00:00

  • Relative contributions of measles virus hemagglutinin- and fusion protein-specific serum antibodies to virus neutralization.

    abstract::The relative contribution of measles virus hemagglutinin (H)- or fusion protein (F)-specific antibodies to virus neutralization (VN) has not been demonstrated. We have depleted these specific antibodies from sera collected from young adults, who had been vaccinated during childhood, by prolonged incubation with viable...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: de Swart RL,Yüksel S,Osterhaus AD

    更新日期:2005-09-01 00:00:00

  • Scavenger receptor BI and BII expression levels modulate hepatitis C virus infectivity.

    abstract::Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) and CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BI and SR-BII, which differ in their C termini. SR-BI...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Grove J,Huby T,Stamataki Z,Vanwolleghem T,Meuleman P,Farquhar M,Schwarz A,Moreau M,Owen JS,Leroux-Roels G,Balfe P,McKeating JA

    更新日期:2007-04-01 00:00:00

  • Tomato bushy stunt virus genomic RNA accumulation is regulated by interdependent cis-acting elements within the movement protein open reading frames.

    abstract::This study on Tomato bushy stunt virus identified and defined three previously unknown regulatory sequences involved in RNA accumulation that are located within the 3'-proximal nested movement protein genes p22 and p19. The first is a 16-nucleotide (nt) element termed III-A that is positioned at the very 3' end of p22...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Park JW,Desvoyes B,Scholthof HB

    更新日期:2002-12-01 00:00:00

  • Localization and Regulation of the T1 Unimolecular Spanin.

    abstract::Spanins are bacteriophage lysis proteins responsible for disruption of the outer membrane, the final step of Gram-negative host lysis. The absence of spanins results in a terminal phenotype of fragile spherical cells. The phage T1 employs a unimolecular spanin gp11 that has an N-terminal lipoylation signal and a C-ter...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Kongari R,Snowden J,Berry JD,Young R

    更新日期:2018-10-29 00:00:00

  • Cas-Br-E murine leukemia virus: sequencing of the paralytogenic region of its genome and derivation of specific probes to study its origin and the structure of its recombinant genomes in leukemic tissues.

    abstract::The ecotropic Cas-Br-E murine leukemia virus (MuLV) and its molecularly cloned derivative pBR-NE-8 MuLV are capable of inducing hind-limb paralysis and leukemia after inoculation into susceptible mice. T1 oligonucleotide fingerprinting, molecular hybridization, and restriction enzyme analysis previously showed that th...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Rassart E,Nelbach L,Jolicoeur P

    更新日期:1986-12-01 00:00:00

  • Role of the SP2 domain and its proteolytic cleavage in HIV-1 structural maturation and infectivity.

    abstract::HIV-1 buds as an immature, noninfectious virion. Proteolysis of its main structural component, Gag, is required for morphological maturation and infectivity and leads to release of four functional domains and the spacer peptides SP1 and SP2. The N-terminal cleavages of Gag and the separation of SP1 from CA are all ess...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: de Marco A,Heuser AM,Glass B,Kräusslich HG,Müller B,Briggs JA

    更新日期:2012-12-01 00:00:00

  • Sequence requirements for Sindbis virus subgenomic mRNA promoter function in cultured cells.

    abstract::The Sindbis virus minimal subgenomic mRNA promoter (spanning positions -19 to +5 relative to the subgenomic mRNA start site) is approximately three- to sixfold less active than the fully active -98 to +14 promoter region. We identified two elements flanking the -19 to +5 region which increase its transcription to leve...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Wielgosz MM,Raju R,Huang HV

    更新日期:2001-04-01 00:00:00

  • Microarray analysis reveals characteristic changes of host cell gene expression in response to attenuated modified vaccinia virus Ankara infection of human HeLa cells.

    abstract::The potential use of the modified vaccinia virus Ankara (MVA) strain as a live recombinant vector to deliver antigens and elicit protective immune responses against infectious diseases demands a comprehensive understanding of the effect of MVA infection on human host gene expression. We used microarrays containing mor...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Guerra S,López-Fernández LA,Conde R,Pascual-Montano A,Harshman K,Esteban M

    更新日期:2004-06-01 00:00:00

  • Analysis of murine hepatitis virus strain A59 temperature-sensitive mutant TS-LA6 suggests that nsp10 plays a critical role in polyprotein processing.

    abstract::Coronaviruses are the largest RNA viruses, and their genomes encode replication machinery capable of efficient replication of both positive- and negative-strand viral RNAs as well as enzymes capable of processing large viral polyproteins into putative replication intermediates and mature proteins. A model described re...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Donaldson EF,Graham RL,Sims AC,Denison MR,Baric RS

    更新日期:2007-07-01 00:00:00

  • Signaling through Toll-like receptors induces murine gammaherpesvirus 68 reactivation in vivo.

    abstract::Murine gammaherpesvirus 68 (MHV68) establishes a lifelong infection in mice and is used as a model pathogen to study the role of viral and host factors in chronic infection. The maintenance of chronic MHV68 infection, at least in some latency reservoirs, appears to be dependent on the capacity of the virus to reactiva...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Gargano LM,Forrest JC,Speck SH

    更新日期:2009-02-01 00:00:00

  • Structural organization of the termini of the L and S components of the genome of pseudorabies virus.

    abstract::The sequences of several hundred nucleotides around the junctions between the L and S components in concatemeric DNA and in mature virion DNA were ascertained. The two ends of the mature genome (which are joined in concatemeric DNA) show no sequence homology. Several directly repeated elements are present near both en...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: DeMarchi JM,Lu ZQ,Rall G,Kupershmidt S,Ben-Porat T

    更新日期:1990-10-01 00:00:00

  • Functional and Structural Insights into a Vif/PPP2R5 Complex Elucidated Using Patient HIV-1 Isolates and Computational Modeling.

    abstract::Human immunodeficiency virus type 1 (HIV-1) Vif recruits a cellular ubiquitin ligase complex to degrade antiviral APOBEC3 enzymes (APOBEC3C-H) and PP2A phosphatase regulators (PPP2R5A to PPP2R5E). While APOBEC3 antagonism is the canonical function of HIV-1 Vif, this viral accessory protein is also known to trigger G2/...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Salamango DJ,McCann JL,Demir Ö,Becker JT,Wang J,Lingappa JR,Temiz NA,Brown WL,Amaro RE,Harris RS

    更新日期:2020-10-14 00:00:00

  • Determinants essential for the transmissible gastroenteritis virus-receptor interaction reside within a domain of aminopeptidase-N that is distinct from the enzymatic site.

    abstract::The swine-specific coronavirus transmissible gastroenteritis virus (TGEV) uses pig aminopeptidase-N (pAPN) as a cellular receptor. We showed that the human aminopeptidase-N (hAPN) cannot substitute for pAPN in this respect, although the two enzymes have 80% amino acid sequence identity. In order to map the TGEV bindin...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Delmas B,Gelfi J,Kut E,Sjöström H,Noren O,Laude H

    更新日期:1994-08-01 00:00:00

  • Optimal replication activity of vesicular stomatitis virus RNA polymerase requires phosphorylation of a residue(s) at carboxy-terminal domain II of its accessory subunit, phosphoprotein P.

    abstract::The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Hwang LN,Englund N,Das T,Banerjee AK,Pattnaik AK

    更新日期:1999-07-01 00:00:00

  • Synthetic double-stranded RNA poly(I:C) combined with mucosal vaccine protects against influenza virus infection.

    abstract::The mucosal adjuvant effect of synthetic double-stranded RNA polyriboinosinic polyribocytidylic acid [poly(I:C)] against influenza virus was examined under intranasal coadministration with inactivated hemagglutinin (HA) vaccine in BALB/c mice and was shown to have a protective effect against both nasal-restricted infe...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Ichinohe T,Watanabe I,Ito S,Fujii H,Moriyama M,Tamura S,Takahashi H,Sawa H,Chiba J,Kurata T,Sata T,Hasegawa H

    更新日期:2005-03-01 00:00:00

  • HIV-1 Coreceptor Usage and Variable Loop Contact Impact V3 Loop Broadly Neutralizing Antibody Susceptibility.

    abstract::In clinical trials, HIV-1 broadly neutralizing antibodies (bnAbs) effectively lower plasma viremia and delay virus reemergence. The presence of less neutralization-susceptible strains prior to treatment decreases the efficacy of these antibody-based treatments, but neutralization sensitivity often cannot be predicted ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Registre L,Moreau Y,Ataca ST,Pulukuri S,Henrich TJ,Lin N,Sagar M

    更新日期:2020-01-06 00:00:00

  • Functional Incompatibility between the Generic NF-κB Motif and a Subtype-Specific Sp1III Element Drives the Formation of the HIV-1 Subtype C Viral Promoter.

    abstract:UNLABELLED:Of the various genetic subtypes of human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV), only in subtype C of HIV-1 is a genetically variant NF-κB binding site found at the core of the viral promoter in association with a subtype-specific Sp1III motif. How the ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Verma A,Rajagopalan P,Lotke R,Varghese R,Selvam D,Kundu TK,Ranga U

    更新日期:2016-07-27 00:00:00

  • A novel sequence-specific DNA-binding protein, LCP-1, interacts with single-stranded DNA and differentially regulates early gene expression of the human neurotropic JC virus.

    abstract::We have identified a novel brain-derived single-stranded-DNA-binding protein that interacts with a region of the human neurotropic JC virus enhancer designated the lytic control element (LCE). This nuclear factor, LCP-1 (for lytic control element-binding protein 1), specifically recognizes the LCE, as determined by ge...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Tada H,Khalili K

    更新日期:1992-12-01 00:00:00

  • Three-dimensional localization of the smallest capsid protein in the human cytomegalovirus capsid.

    abstract::The smallest capsid proteins (SCPs) of the human herpesviruses differ substantially in size and sequence and are thought to impart some unique aspects of infection to their respective viruses. We used electron cryomicroscopy and antibody labeling to show that the 8-kDa SCP of human cytomegalovirus is attached only to ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Yu X,Shah S,Atanasov I,Lo P,Liu F,Britt WJ,Zhou ZH

    更新日期:2005-01-01 00:00:00

  • Heterogeneity of envelope molecules expressed on primary human immunodeficiency virus type 1 particles as probed by the binding of neutralizing and nonneutralizing antibodies.

    abstract::Virion capture assays, in which immobilized antibodies (Abs) capture virus particles, have been used to suggest that nonneutralizing Abs bind effectively to human immunodeficiency virus type 1 (HIV-1) primary viruses. Here, we show that virion capture assays, under conditions commonly reported in the literature, give ...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Poignard P,Moulard M,Golez E,Vivona V,Franti M,Venturini S,Wang M,Parren PW,Burton DR

    更新日期:2003-01-01 00:00:00

  • Mutagenesis analysis of the nsp4 main proteinase reveals determinants of arterivirus replicase polyprotein autoprocessing.

    abstract::Nonstructural protein 4 (nsp4; 204 amino acids) is the chymotrypsin-like serine main proteinase of the arterivirus Equine arteritis virus (order Nidovirales), which controls the maturation of the replicase complex. nsp4 includes a unique C-terminal domain (CTD) connected to the catalytic two-beta-barrel structure by t...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: van Aken D,Snijder EJ,Gorbalenya AE

    更新日期:2006-04-01 00:00:00

  • Transfer of a mutant dihydrofolate reductase gene into pre- and postimplantation mouse embryos by a replication-competent retrovirus vector.

    abstract::In order to explore the potential of retrovirus vectors for efficiently transferring foreign genes into mouse embryos, a replication-competent recombinant Moloney murine leukemia virus (Mo-MLV) vector carrying a mutant dihydrofolate reductase (DHFR) cDNA insert in the U3 region of the viral long terminal repeat was us...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Stuhlmann H,Jaenisch R,Mulligan RC

    更新日期:1989-11-01 00:00:00

  • Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA.

    abstract::The 5'-terminal nucleotide sequence from positions 50 to 130 of vesicular stomatitis virus RNA was determined indirectly by using a defective interfering particle RNA which contains covalently linked genomic minus and antigenomic plus sense RNAs. The last 18 nucleotides of the L gene coding for in the viral polymerase...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Schubert M,Keene JD,Herman RC,Lazzarini RA

    更新日期:1980-05-01 00:00:00

  • Cooperative transformation and coexpression of bovine papillomavirus type 1 E5 and E7 proteins.

    abstract::Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the b...

    journal_title:Journal of virology

    pub_type: 杂志文章


    authors: Bohl J,Hull B,Vande Pol SB

    更新日期:2001-01-01 00:00:00