Abstract:
:Recent studies suggest the existence of cancer stem cells (CSC) and cancer progenitor cells (CPC), although strict definitions of neither CSC nor CPC have been developed. We have produced evidence that the principal oncoprotein of Epstein-Barr virus (EBV), latent membrane protein 1 (LMP1), which is associated with human malignancies, especially nasopharyngeal carcinoma (NPC), promotes tumor cell invasion and metastasis, as well as the epithelial-mesenchymal transition (EMT). However, whether LMP1 is involved in the development of CSC/CPC is still unclear. This study investigates whether the expression of EBV-LMP1 is related to the development of CSC/CPC. Analysis of cancer stem cell markers reveals that LMP1 induces the CD44(high) CD24(low) CSC/CPC-like phenotype as well as self-renewal abilities in LMP1-expressing epithelial cell lines. In addition, we show here that LMP1 induction in epithelial cells causes high tumorigenicity and rapid cellular proliferation. Furthermore, we found that LMP1 expression increased the expression of several CPC markers as well as producing increased levels of EMT markers. Our findings indicate that LMP1 can induce a CPC-like rather than a CSC-like phenotype in epithelial cells and suggest that LMP1-induced phenotypic changes contribute to the development of NPC.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kondo S,Wakisaka N,Muramatsu M,Zen Y,Endo K,Murono S,Sugimoto H,Yamaoka S,Pagano JS,Yoshizaki Tdoi
10.1128/JVI.00188-11subject
Has Abstractpub_date
2011-11-01 00:00:00pages
11255-64issue
21eissn
0022-538Xissn
1098-5514pii
JVI.00188-11journal_volume
85pub_type
杂志文章abstract::Previous analyses have demonstrated that adenovirus DNA is packaged into virions in vivo in a polar, left-to-right fashion. The packaging of viral DNA is dependent on cis-acting elements at the left end of the genome. In this report, we describe a genetic analysis of the sequences that are required for efficient packa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.5.2047-2056.1990
更新日期:1990-05-01 00:00:00
abstract::The locations and functions of DNA sequences involved in transcription of the gene encoding herpes simplex virus type 1 immediate early (IE) mRNAs 4 and 5 were analyzed by use of a transient-expression assay. The region upstream of the genes encoding IE mRNAs 4 and 5 was fused to the thymidine kinase gene coding seque...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.50.3.708-716.1984
更新日期:1984-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.18.12045-12057.2005
更新日期:2005-09-01 00:00:00
abstract::The Epstein-Barr virus (EBV) genome contains an open reading frame, BHRF1, that encodes a presumptive membrane protein with sequence similarity to the proto-oncogene bcl2, which is linked to human B-cell follicular lymphoma. Potential roles for BHRF1 in EBV's ability to growth transform human B cells and to replicate ...
journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.9.3399-3406.1988
更新日期:1988-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.33.3.1199-1202.1980
更新日期:1980-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1984-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.5.2879-2886.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.2.709-716.1976
更新日期:1976-08-01 00:00:00
abstract::Since 2005, a large poliomyelitis outbreak associated with type 2 circulating vaccine-derived poliovirus (cVDPV2) has occurred in northern Nigeria, where immunization coverage with trivalent oral poliovirus vaccine (tOPV) has been low. Phylogenetic analysis of P1/capsid region sequences of isolates from each of the 40...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02954-12
更新日期:2013-05-01 00:00:00
abstract:UNLABELLED:Previous experiments carried out in a sheep scrapie model demonstrated that the transfusion of 200 μl of prion-infected whole blood has an apparent 100% efficacy for disease transmission. These experiments also indicated that, despite the apparent low infectious titer, the intravenous administration of white...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02783-15
更新日期:2016-01-13 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2020-01-31 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2020-10-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.3.533-542.1977
更新日期:1977-09-01 00:00:00
abstract::VP16-H is an essential structural protein of herpes simplex virus type 1 (HSV-1) and is also a potent activator of virus immediate-early (IE) gene expression. Current models of functional determinants within VP16-H indicate that it consists of two domains, an N-terminal domain involved in recruiting VP16-H to a multic...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2000-11-01 00:00:00
abstract::Studies on deoxyribonucleic acid purified from the granulosis virus of Trichoplusia ni have revealed the presence of a closed, double-stranded superhelix which sediments at 95S relative to relaxed circles (74S) and linear (60S) forms. Molecular weight estimates show that this insect virus deoxyribonucleic acid has a s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.710-713.1972
更新日期:1972-04-01 00:00:00
abstract::We have previously shown that styrylquinolines (SQLs) are integrase inhibitors in vitro. They compete with the long terminal repeat substrate for integrase. Here, we describe the cellular mode of action of these molecules. We show that SQLs do not interfere with virus entry. In fact, concentrations of up to 20 times t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.11.5728-5736.2004
更新日期:2004-06-01 00:00:00
abstract::Virus was isolated from the lumen of the calyx region of ovaries in the parasitoid wasp Campoletis sonorensis (Hymenoptera: Ichneumonidae), and the nature of the viral DNA was analyzed. DNA purified from a homogeneous band of virus contained double-stranded superhelical molecules which were polydisperse in molecular w...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.7.4283-4290.1996
更新日期:1996-07-01 00:00:00
abstract::Dengue virus (DENV) is a mosquito-borne virus that infects upward of 300 million people annually and has the potential to cause fatal hemorrhagic fever and shock. While the parameters contributing to dengue immunopathogenesis remain unclear, the collapse of redox homeostasis and the damage induced by oxidative stress ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01551-20
更新日期:2020-11-23 00:00:00
abstract::The genome of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 rescued by passage on transformed permissive monkey lines (see accompanying paper [Y. Gluzman et al., J. Virol. 24:534-540, 1977]) was analyzed by restriction endonuclease cleavage mapping to obtain biochemical evidence that the rescue o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.24.2.541-550.1977
更新日期:1977-11-01 00:00:00