Abstract:
:In the spherical capsid of hepatitis B virus (HBV), intermolecular disulfide bonds cross-link the approximately 180 p21.5 capsid protein subunits into a stable lattice. In this study, we used mutant capsid proteins to investigate the role that disulfide bonds and the four p21.5 Cys residues (positions 48, 61, 107, and 185) play in capsid assembly and/or stabilization. p21.5 Cys residues were either replaced by Ala or removed (Cys-185) by carboxyl-terminal truncation, creating Cys-minus mutants which were expressed in Xenopus oocytes via microinjected synthetic mRNAs. Fractionation of radiolabeled oocyte extracts on 10 to 60% sucrose gradients revealed that Cys-minus core proteins resolved into the nonparticulate and capsid forms seen for wild-type p21.5. On 5 to 30% sucrose gradients, nonparticulate Cys-minus core proteins sedimented as dimers of approximately 40 kDa. We conclude that Cys residues and disulfides are not required for the assembly of either HBV capsids or the dimers that provide the precursors for capsid assembly. Since assembly presumably demands an appropriate p21.5 tertiary structure, it is unlikely that Cys residues are required for proper p21.5 folding. However, Cys residues stabilize isolated p21.5 structures, as evidenced by the marked reduction in stability of Cys-minus dimers and capsids (i) in nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis and (ii) upon protease digestion. We discuss these results in the context of the HBV life cycle and the role of Cys residues in other proteins.
journal_name
J Viroljournal_title
Journal of virologyauthors
Zhou S,Standring DNdoi
10.1128/JVI.66.9.5393-5398.1992subject
Has Abstractpub_date
1992-09-01 00:00:00pages
5393-8issue
9eissn
0022-538Xissn
1098-5514journal_volume
66pub_type
杂志文章abstract::Hepatitis C virus (HCV) infection is believed to begin with interactions between cell-free HCV and cell receptors that include CD81, scavenger receptor B1 (SR-B1), claudin-1 (CLDN1), and occludin (OCLN). In this study, we have demonstrated that HCV spreading from infected hepatocytes to uninfected hepatocytes leads to...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01062-13
更新日期:2013-08-01 00:00:00
abstract::The envelope of human parainfluenza virus type 3 (HPF3) contains two viral glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion protein (F). HN, which is responsible for receptor attachment and for promoting F-mediated fusion, also possesses neuraminidase (receptor-destroying) activity. We reported previ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7481-7488.2001
更新日期:2001-08-01 00:00:00
abstract::AIDS-related human cytomegalovirus retinitis remains the leading cause of blindness among untreated HIV/AIDS patients worldwide. To study mechanisms of this disease, we used a clinically relevant animal model of murine cytomegalovirus (MCMV) retinitis with retrovirus-induced murine AIDS (MAIDS) that mimics the progres...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00526-18
更新日期:2018-08-29 00:00:00
abstract::We previously showed that murine polyomavirus mutants that lack both middle T (MT) and small T (ST) functions have a severe pleiotropic defect in early and late viral gene expression as well as genome amplification. The respective contribution of MT and ST to this phenotype was unclear. This work separates the roles o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00679-06
更新日期:2006-08-01 00:00:00
abstract::The feline immunodeficiency virus (FIV) cat model is extensively used to investigate possible vaccination approaches against AIDS in humans. Although consistent levels of protection have been achieved with FIV, as with other model systems, by immunizing with whole inactivated virus or fixed infected cells, the mechani...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.1.1-10.1999
更新日期:1999-01-01 00:00:00
abstract::Bacteriophage CL31 was isolated on a Corynebacterium lilium strain. Out of 30 strains tested, only CL31 was able to form plaques on Corynebacterium glutamicum ATCC 13287, Brevibacterium lactofermentum ATCC 21086, and Arthrobacter sp. strain SI55, but at a very low frequency. This phage belongs to group B of Bradley's ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.5.1540-1545.1987
更新日期:1987-05-01 00:00:00
abstract::Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7791-7799.1995
更新日期:1995-12-01 00:00:00
abstract::SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01246-16
更新日期:2016-11-14 00:00:00
abstract::Vaccinia virus assembles two distinct lipoprotein membranes. The primary membrane contains nonglycosylated proteins, appears as crescents in the cytoplasm, and delimits immature and mature intracellular virions. The secondary or wrapping membrane contains glycoproteins, is derived from virus-modified trans-Golgi or en...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.21.11754-11766.2003
更新日期:2003-11-01 00:00:00
abstract::The retroviral restriction factor TRIMCyp, derived from the TRIM5 gene, blocks replication at a postentry step. TRIMCyp has so far been found in four species of Asian macaques, Macaca fascicularis, M. mulatta, M. nemestrina, and M. leonina. M. fascicularis is commonly used as a model for AIDS research, but TRIMCyp has...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00097-11
更新日期:2011-10-01 00:00:00
abstract::Cyclooxygenase-2 (COX-2) is a cellular enzyme in the eicosanoid synthetic pathway that mediates the synthesis of prostaglandins from arachidonic acid. The eicosanoids function as critical regulators of a number of cellular processes, including the acute and chronic inflammatory response, hemostasis, and the innate imm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.22.12529-12536.2004
更新日期:2004-11-01 00:00:00
abstract:UNLABELLED:Multiple cellular pathways are regulated by small ubiquitin-like modifier (SUMO) modification, including ubiquitin-mediated proteolysis, signal transduction, innate immunity, and antiviral defense. In the study described in this report, we investigated the effects of SUMO on the replication of two members of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00722-16
更新日期:2016-06-24 00:00:00
abstract::T-antigen-induced DNA distortion was studied in a series of simian virus 40 (SV40) plasmid constructs whose relative replication efficiency ranges from 0.2 to 36. Bending was detected in the wild-type SV40 regulatory region consisting of three copies of the GC-rich 21-bp repeat but not in constructs with only one or t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10651-10657.2003
更新日期:2003-10-01 00:00:00
abstract::The classical swine fever virus (CSFV) live attenuated vaccine C-strain is adaptive to rabbits and attenuated in pigs, in contrast with the highly virulent CSFV Shimen strain. Previously, we demonstrated that P108 and T109 on the E2 glycoprotein (E2P108-T109) in domain I (E2DomainI) rather than R132, S133, and D191 in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01104-20
更新日期:2020-08-17 00:00:00
abstract::Cells latently infected with herpes simplex virus (HSV) contain nucleosomal DNA similar to that of host cell chromatin. Recent studies have demonstrated that histones in the latency-associated transcript (LAT) promoter and intron regions contain histone modifications permissive for transcription. However, those histon...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02447-06
更新日期:2007-05-01 00:00:00
abstract::The lack of a mouse model has hampered an understanding of the pathogenesis and immunity of Marburg hemorrhagic fever (MHF), the disease caused by marburgvirus (MARV), and has created a bottleneck in the development of antiviral therapeutics. Primary isolates of the filoviruses, i.e., ebolavirus (EBOV) and MARV, are n...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00126-09
更新日期:2009-07-01 00:00:00
abstract::A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.61-68.1977
更新日期:1977-01-01 00:00:00
abstract::Oligonucleotide-directed mutagenesis was used to construct a nonsense mutation in open reading frame (ORF) E2 of bovine papillomavirus DNA. A single base substitution mutation was constructed which converted a TAC codon into a TAG amber stop codon at a position in the ORF that did not overlap with any other viral ORFs...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.2.475-480.1986
更新日期:1986-02-01 00:00:00
abstract::Inhibition of host-directed gene expression by the matrix (M) protein of vesicular stomatitis virus (VSV) effectively blocks host antiviral responses, promotes virus replication, and disables the host cell. However, dendritic cells (DC) have the capacity to resist these effects and remain functional during VSV infecti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00680-13
更新日期:2013-11-01 00:00:00
abstract:UNLABELLED:Three waves of human infection with H7N9 influenza viruses have concluded to date, but only viruses within the first wave (isolated between March and September 2013) have been extensively studied in mammalian models. While second- and third-wave viruses remain closely linked phylogenetically and antigenicall...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00134-16
更新日期:2016-04-14 00:00:00
abstract::Bovine leukemia virus (BLV) and human T-cell lymphotropic virus type 1 (HTLV-1) belong to the genus of deltaretroviruses. Their entry into the host cell is supposed to be mediated by interactions of the extracellular (SU) envelope glycoproteins with cellular receptors. To gain insight into the mechanisms governing thi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.7956-7967.2002
更新日期:2002-08-01 00:00:00
abstract::Herpesviruses establish latency in suitable host cells after primary infection and persist in their host organisms for life. Most of the viral genes are silenced during latency, also enabling the virus to escape from an immune response. This study addresses the control of viral gene silencing by epigenetic mechanisms,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00164-11
更新日期:2011-06-01 00:00:00
abstract::Substitution of a methionine residue at position 79 in poliovirus protein 3A with valine or threonine caused defective viral RNA synthesis, manifested as delayed onset and reduced yield of viral RNA, in HeLa cells transfected with a luciferase-containing replicon. Viruses containing these same mutations produced small...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.23.12679-12691.2003
更新日期:2003-12-01 00:00:00
abstract::Cloned DNA from the P155 mutant of polyoma virus transforms cells in culture as efficiently as wild-type DNA, but has a much lower tumorigenic potential when injected into newborn rats. Like cells transformed by wild-type DNA, cells transformed by the mutant DNA grow in low serum concentrations, form colonies in agar ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.1072-1081.1982
更新日期:1982-09-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) remains latent throughout the life of the carrier, with cells containing the provirus and viral gene expression efficiently down-regulated. On a molecular level, exactly how viruses are down-regulated in vivo remains unresolved. We described here the possibility that down-re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4485-4494.1997
更新日期:1997-06-01 00:00:00
abstract::The present study used the cottontail rabbit papillomavirus DNA-rabbit system to evaluate whether the regulatory genes E1 and E2 and the transforming gene E6 are required for papilloma formation. Frameshift mutations were generated in the individual genes in the context of a full-length cottontail rabbit papillomaviru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.6097-6102.1994
更新日期:1994-09-01 00:00:00
abstract::Zyxin, a focal adhesion molecule, interacts specifically with the E6 protein from human papillomavirus (HPV) type 6 in a yeast two-hybrid screen of a cDNA library prepared from human keratinocytes. Zyxin does not interact significantly with E6 proteins from HPV types 11, 16, or 18. The interaction was confirmed by in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11791-11802.2001
更新日期:2001-12-01 00:00:00
abstract::Only a few archaeal viruses have been subjected to detailed structural analyses. Major obstacles have been the extreme conditions such as high salinity or temperature needed for the propagation of these viruses. In addition, unusual morphotypes of many archaeal viruses have made it difficult to obtain further informat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01347-09
更新日期:2010-01-01 00:00:00
abstract::Genetic and biochemical studies have suggested the existence of a bacteriophage-like, DNA-packaging/ejecting portal complex in herpesviruses capsids, but its arrangement remained unknown. Here, we report the first visualization of a unique vertex in the Kaposi's sarcoma-associated herpesvirus (KSHV) capsid by cryoelec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02254-06
更新日期:2007-04-01 00:00:00
abstract::The identification of several simian immunodeficiency virus mac251 (SIV(mac251)) cytotoxic T-lymphocyte epitopes recognized by CD8(+) T cells of infected rhesus macaques carrying the Mamu-A*01 molecule and the use of peptide-major histocompatibility complex tetrameric complexes enable the study of the frequency, bread...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11483-11495.2001
更新日期:2001-12-01 00:00:00