Abstract:
:Carcinogenic progression of a human papillomavirus (HPV)-infected cell is often associated with integration of the viral genome in a manner which results in the loss of expression of the viral regulatory protein E2. One function of E2 is the regulation of expression of the viral oncogenes, E6 and E7. Introduction of the bovine papillomavirus type 1 (BPV-1) E2 transactivator (E2-TA) in HeLa cells, an HPV type 18 (HPV-18)-positive cervical carcinoma cell line results in growth arrest. In this study, we have found that the HPV-16 and HPV-18 E2 proteins share with BPV-1 E2-TA the ability to suppress HeLa cell growth. This property was not observed for the BPV-1 E2 transcriptional repressor (E2-TR). Analysis of various mutant E2 proteins for growth suppression revealed a requirement for the intact transactivation and DNA binding domains. A HeLa cell line (HeLa-tsE2) which expressed a conditional mutant E2 protein that was functional only at the permissive temperature (32 degrees C) was established, permitting an analysis of the molecular and cellular consequences of E2 expression. Our data indicate that one mechanism by which E2 suppresses cellular growth is through repression of E6 and E7 expression, thereby enabling the cellular targets of E6 and E7 to resume regulation of the cell cycle.
journal_name
J Viroljournal_title
Journal of virologyauthors
Dowhanick JJ,McBride AA,Howley PMdoi
10.1128/JVI.69.12.7791-7799.1995subject
Has Abstractpub_date
1995-12-01 00:00:00pages
7791-9issue
12eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract::Glycans on human immunodeficiency virus (HIV) envelope protein play an important role in infection and evasion from host immune responses. To examine the role of specific glycans, we introduced single or multiple mutations into potential N-linked glycosylation sites in hypervariable regions (V1 to V3) of the env gene ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01691-07
更新日期:2008-01-01 00:00:00
abstract::Recombination studies have established that retroviral long terminal repeats (LTRs) are important genetic determinants of the viral capacity to induce hematopoietic tumors and to specify the type of cell making up the tumor. Plasmids containing LTRs of several murine leukemia viruses linked to the chloramphenicol acet...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.4.1067-1072.1987
更新日期:1987-04-01 00:00:00
abstract::Sequence analysis of the short unique (Us) segment of the bovine herpesvirus 1 (BHV-1) genome predicted that the Us open reading frame (ORF) 4 encodes a protein with homology to glycoprotein G (gG) of other alpha-herpesviruses (P. Leung-Tack, J.-C. Audonnet, and M. Riviere, Virology 199:409-421, 1994). RNA analysis sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.5.3032-3038.1996
更新日期:1996-05-01 00:00:00
abstract::The genome of a canine parvovirus isolate strain (CPV-N) was cloned, and the DNA sequence was determined. The entire genome, including ends, was 5,323 nucleotides in length. The terminal repeat at the 3' end of the genome shared similar structural characteristics but limited homology with the rodent parvoviruses. The ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.1.266-276.1988
更新日期:1988-01-01 00:00:00
abstract::Analysis of the pronase-derived glycopeptides of isolated mumps virus glycoproteins revealed the presence of both complex and high-mannose-type oligosaccharides on the HN and F1 glycoproteins, whereas only high-mannose-type glycopeptides were detected on F2. Endoglycosidase F, a newly described glycosidase that cleave...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.354-362.1983
更新日期:1983-08-01 00:00:00
abstract::The administration of cyclophosphamide (50 to 100 mg/kg) at 48 to 72 h before removal of murine lung or spleen mononuclear cells for culture rendered DBA/2 mice incapable of generating an effective cytotoxic T-lymphocyte response to influenza A virus-infected cells. The cytotoxic T-lymphocyte precursor frequency to in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.20-25.1984
更新日期:1984-07-01 00:00:00
abstract:UNLABELLED:Identification and characterization of CD8(+) T cells effectively controlling HIV-1 variants are necessary for the development of AIDS vaccines and for studies of AIDS pathogenesis, although such CD8(+) T cells have been only partially identified. In this study, we sought to identify CD8(+) T cells controlli...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00020-15
更新日期:2015-05-01 00:00:00
abstract::A coxsackievirus B3 (CB3) isolate adapted to growth in RD cells shows an alteration in cell tropism as a result of its capacity to bind a 70-kDa cell surface molecule expressed on these cells. We now show that this molecule is the complement regulatory protein, decay-accelerating factor (DAF) (CD55). Anti-DAF antibodi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.3.1903-1906.1995
更新日期:1995-03-01 00:00:00
abstract::The widespread presence of endogenous retroviruses in the genomes of animals and humans has suggested that these viruses may be involved in both normal and abnormal developmental processes. Previous studies have indicated the involvement of endogenous ecotropic murine leukemia virus (MuLV) in the development of age-de...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4362-4369.1989
更新日期:1989-10-01 00:00:00
abstract::A sequence of polyadenylic acid, homogeneous in composition but heterogeneous in length, was isolated from complete pancreatic RNase digests of both middle and bottom RNAs of cowpea mosaic virus. The polyadenylic acid was 3'-terminal and occurred once per molecule. A fragment consisting of the polyadenylic acid and ap...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.26.2.243-248.1978
更新日期:1978-05-01 00:00:00
abstract:UNLABELLED:SSV-type integrases, encoded by fuselloviruses which infect the hyperthermophilic archaea of the Sulfolobales, are archaeal members of the tyrosine recombinase family. These integrases catalyze viral integration into and excision from a specific site on the host genome. In the present study, we have establis...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01637-15
更新日期:2015-11-01 00:00:00
abstract::The host cell receptor for Moloney murine leukemia virus was solubilized from murine L-cell membranes and characterized. In initial studies designed to identify a receptor-rich cell line, different mouse cells were screened for binding to Moloney gp70, the viral envelope glycoprotein which determines host cell-binding...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.58.3.900-908.1986
更新日期:1986-06-01 00:00:00
abstract::Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. In the present study, we have investigat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.3.1764-1771.2003
更新日期:2003-02-01 00:00:00
abstract::The functions of the two rotavirus glycoproteins were investigated by using tunicamycin and a variant of SA11 rotavirus having nonglycosylated VP7. Results showed that glycosylation of VP7 is not required for normal viral morphogenesis and infectivity and suggested that the nonstructural glycoprotein is involved in as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.1.270-274.1983
更新日期:1983-04-01 00:00:00
abstract::A new subgroup of avian leukosis virus (ALV) that includes a unique env gene, designated J, was identified recently in England. Sequence analysis of prototype English isolate HPRS-103 revealed several other unique genetic characteristics of this strain and provided information that it arose by recombination between ex...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10301-10304.1998
更新日期:1998-12-01 00:00:00
abstract::The helicase-like 1a and polymerase-like 2a proteins of brome mosaic virus (BMV) are required for viral RNA replication in vivo, are present in membrane-bound viral RNA polymerase extracts, and share conservation with the many other members of the alphavirus-like superfamily. To better understand BMV RNA replication a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8908-8916.1996
更新日期:1996-12-01 00:00:00
abstract:UNLABELLED:Three waves of human infection with H7N9 influenza viruses have concluded to date, but only viruses within the first wave (isolated between March and September 2013) have been extensively studied in mammalian models. While second- and third-wave viruses remain closely linked phylogenetically and antigenicall...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00134-16
更新日期:2016-04-14 00:00:00
abstract::The various strains of BK virus (BKV) exhibit a remarkable degree of heterogeneity in the transcriptional control region, which may affect the biological characteristics of a BKV strain. We describe the detection and sequencing of BKV control regions directly from urine samples and after propagation in cell culture. A...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.8.3864-3871.1990
更新日期:1990-08-01 00:00:00
abstract::The nr allele at the mouse Fv1 restriction locus governs resistance to B-tropic and some N-tropic murine leukemia viruses (MLVs). Sequence analysis and site-specific mutagenesis of N-tropic MLVs identified a single amino acid difference responsible for this restriction that is distinct from the site that governs N or ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5385-5387.2000
更新日期:2000-06-01 00:00:00
abstract::Adeno-associated viruses (AAVs) are small single-stranded DNA viruses that can package and deliver nongenomic DNA for therapeutic gene delivery. AAV8, a liver-tropic vector, has shown great promise for the treatment of hemophilia A and B. However, as with other AAV vectors, host anti-capsid immune responses are a dete...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00218-12
更新日期:2012-08-01 00:00:00
abstract::Adenovirus type 35 (Ad35) is a group B adenovirus that has been isolated primarily from patients with acquired immunodeficiency syndrome and other immunodeficiency disorders. We have studied the interaction of this unique adenovirus with the immune system by analyzing Ad35 early viral proteins in infected HeLa cells. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.12.3665-3671.1987
更新日期:1987-12-01 00:00:00
abstract::In vitro studies have indicated that retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor template. Increasing the rate of DNA synthesis is expected to reduce the frequency of RT template switching, whereas annealing the nascen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.15.7473-7484.2002
更新日期:2002-08-01 00:00:00
abstract::DNA replication intermediates of three plasmids containing all or part of a modified Epstein-Barr virus cis-acting plasmid maintenance region (oriP) were examined to further investigate oriP function. Replication intermediates were analyzed in vivo and in vitro by neutral-neutral two-dimensional gel electrophoresis. T...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1739-1745.1993
更新日期:1993-03-01 00:00:00
abstract::Intracellular simian virus 40 (SV40) chromatin was photoreacted with a 3H-labeled psoralen derivative, hydroxymethyltrimethylpsoralen (HMT), at 48 h postinfection. Psoralen compounds have been shown to readily penetrate intact cells and, in the presence of long-wavelength UV light, form covalent adducts to DNA, prefer...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.1.78-87.1982
更新日期:1982-01-01 00:00:00
abstract::The required activities of CD4(+) T cells and antibody against the virally encoded oncoprotein simian virus 40 (SV40) Tag have previously been demonstrated by our laboratory to be mediators in achieving antitumor responses and tumor protection through antibody-dependent cell-mediated cytotoxicity (ADCC). In this study...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01512-09
更新日期:2010-01-01 00:00:00
abstract::Shrimp subcuticular epithelial cells are the initial and major targets of white spot syndrome virus (WSSV) infection. Proteomic studies of WSSV-infected subcuticular epithelium of Penaeus monodon were performed through two approaches, namely, subcellular fractionation coupled with shotgun proteomics to identify viral ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01006-07
更新日期:2007-11-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to several neoplastic diseases: Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD). KSHV replicates actively, via a controlled gene expression program, but can also remain latent. It had been thought that the transition...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06617-11
更新日期:2012-04-01 00:00:00
abstract::Transgenic mice containing the complete human immunodeficiency virus (HIV) coding sequences fused to the mouse mammary tumor virus long terminal repeat were generated. They were found to produce high levels of authentic gag and env HIV proteins in several tissues known to support mouse mammary tumor virus-driven trans...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.6.3904-3908.1992
更新日期:1992-06-01 00:00:00
abstract::To analyze the proposed growth-inhibitory function of wild-type p53, we compared simian virus 40 (SV40) DNA replication in primary rhesus monkey kidney (PRK) cells, which express wild-type p53, and in the established rhesus monkey kidney cell line LLC-MK2, which expresses a mutated p53 that does not complex with large...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.2.886-893.1993
更新日期:1993-02-01 00:00:00
abstract::The structure of recombinant virus-like particles of malabaricus grouper nervous necrosis virus (MGNNV), a fish nodavirus isolated from the grouper Epinephelus malabaricus, was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-A resolution. The cryoEM structure, sequence compari...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.12.6370-6375.2002
更新日期:2002-06-01 00:00:00