Abstract:
:Adenovirus type 35 (Ad35) is a group B adenovirus that has been isolated primarily from patients with acquired immunodeficiency syndrome and other immunodeficiency disorders. We have studied the interaction of this unique adenovirus with the immune system by analyzing Ad35 early viral proteins in infected HeLa cells. We have identified a 29,000-Mr Ad35 early glycoprotein, E29, which associates with class I antigens of the major histocompatibility complex (MHC) in the endoplasmic reticulum. Ad35 E29 is analogous to the group C Ad2 early glycoprotein E3-19K (E19), which has been shown to interfere with the expression of class I antigens on the cell surface (H. Burgert and S. Kvist, Cell 41:987-997, 1985). In contrast to the Ad2 glycoprotein, Ad35 E29 was synthesized in much smaller amounts, was more extensively glycosylated, and did not cross-react with polyclonal antibody against the Ad2 protein. As a control, a class I antigen-binding glycoprotein from another group B adenovirus, Ad7, was also characterized and was found to have properties similar to those of Ad35 E29. Therefore, the differences in the glycosylation and quantity of class I antigen-binding glycoproteins between Ad35 and Ad2 are group related. Inhibition of the expression of MHC class I antigens, which are needed for cytotoxic-T-lymphocyte recognition of virus-infected cells, appears to play a vital role in the adenovirus life cycle in vivo. Our data indicate that this function has been conserved despite significant differences in the MHC class I antigen-binding glycoprotein and in the pathogenicity between serotypes.
journal_name
J Viroljournal_title
Journal of virologyauthors
Flomenberg PR,Chen M,Horwitz MSdoi
10.1128/JVI.61.12.3665-3671.1987subject
Has Abstractpub_date
1987-12-01 00:00:00pages
3665-71issue
12eissn
0022-538Xissn
1098-5514journal_volume
61pub_type
杂志文章abstract::Bovine papilloma virus (BPV) appears to be the etiological agent of common equine connective tissue tumors. We investigated the physical state of the viral DNA within such tumors and found no indication for integration into the host genome. The BPV genomes were present as free circular episomes. Two equine sarcoids we...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.962-964.1980
更新日期:1980-09-01 00:00:00
abstract::The initiation of RNA transcription by the virion-bound RNA transcriptase of vesicular stomatitis virus has been examined. Multiple initiation sequences have been observed, two of which have been characterized (pppApCpGp... and pppGpCp...) suggestive of a transcription process which can start at different sites along ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.11.4.487-501.1973
更新日期:1973-04-01 00:00:00
abstract::Autoradiographic analyses of deoxyribonucleic acid (DNA) synthesis in randomly growing KB cell cultures infected with equine abortion virus (EAV) suggested that viral DNA synthesis was initiated only at times that coincided with the entry of noninfected control cells into the S phase of the cell cycle. Synchronized cu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.6.736-748.1971
更新日期:1971-06-01 00:00:00
abstract::The monoclonal antibody PAb280 binds to small t antigen but not to large T antigen. Its binding site within the unique region of small t antigen was localized by studying its reaction with simian virus 40 mutants, other papovaviruses, and bacterial expression vectors coding for fragments of small t antigen. The antibo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.7.3128-3134.1989
更新日期:1989-07-01 00:00:00
abstract::In this paper, we report that the DNA of bovine mammillitis virus (BMV) consists of two covalently linked components that are 71.5 x 10(6) and 15.7 x 10(6) in molecular weight and designated L and S, respectively. We further report that the BMV DNA consists of four equimolar populations differing only in the orientati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.25.1.395-407.1978
更新日期:1978-01-01 00:00:00
abstract::In previous studies, we demonstrated that single-chain variable fragments (scFvs) from anti-human immunodeficiency virus (HIV) Env monoclonal antibodies act as entry inhibitors when tethered to the surface of target cells by a glycosyl-phosphatidylinositol (GPI) anchor. Interestingly, even if a virus escapes inhibitio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-17
更新日期:2018-03-14 00:00:00
abstract::Crucial steps in high-risk human papillomavirus (HR-HPV)-related carcinogenesis are the integration of HR-HPV into the host genome and loss of viral episomes. The mechanisms that promote cervical neoplastic progression are, however, not clearly understood. During HR-HPV infection, the HPV E5 protein is expressed in pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02114-10
更新日期:2011-05-01 00:00:00
abstract::Some paramyxovirus V proteins induce STAT protein degradation, and the amino acids essential for this process in the human parainfluenza virus type 2 (hPIV2) V protein have been studied. Various recombinant hPIV2s and cell lines constitutively expressing various mutant V proteins were generated. We found that V protei...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.13.8591-8601.2005
更新日期:2005-07-01 00:00:00
abstract::To localize the epitopes recognized by monoclonal antibodies (MAbs) specific for the S1 subunit of the murine coronavirus JHMV spike protein, we have expressed S1 proteins with different deletions from the C terminus of S1. S1utt is composed of the entire 769-amino-acid (aa) S1 protein; S1NM, S1N, S1n(330), and S1n(22...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.9.5403-5410.1994
更新日期:1994-09-01 00:00:00
abstract::Abnormal prion protein (PrP(Sc)) plays a central role in the transmission of prion diseases, but the molecular basis of prion strains with distinct biological characteristics remains to be elucidated. We analyzed the characteristics of prion disease by using mice inoculated with the Chandler and Fukuoka-1 strains prop...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.11.7104-7112.2005
更新日期:2005-06-01 00:00:00
abstract::We have demonstrated that Epstein-Barr virus (EBV) confers enhanced growth capability in soft agarose, tumorigenesis in the SCID mouse, and resistance to apoptosis in the Burkitt's lymphoma cell line Akata. Subsequently, we have shown that EBV-encoded small RNAs (EBERs) are responsible for these phenotypes. We constan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.3.1561-1564.2001
更新日期:2001-02-01 00:00:00
abstract::The temperature-sensitive defects of virus mutants isolated from L cells persistently infected with Newcastle disease virus (NDV) were analyzed. Genetic grouping of the mutants by complementation tests was attempted by using several different methods, including yield analysis, RNA synthesis, and heterozygote formation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.472-480.1973
更新日期:1973-09-01 00:00:00
abstract::Gammaherpesvirus 68 (gammaHV68, or MHV68) is a naturally occurring rodent pathogen that replicates to high titer in cell culture and is amenable to in vivo experimental evaluation of viral and host determinants of gammaherpesvirus disease. However, the inability of MHV68 to transform primary murine B cells in culture,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02689-07
更新日期:2008-08-01 00:00:00
abstract::The enhancer within the long terminal repeats (LTRs) of acquired somatic mouse mammary tumor viruses (MMTV) can activate juxtaposed genes and induce mammary tumors. In contrast, germ line proviral MMTV genomes are integrated in the host genome and considered to be genetically confined transcription units. Here we demo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.10.8669-8676.1999
更新日期:1999-10-01 00:00:00
abstract::Productively infected bovine fibropapillomas were examined for bovine papillomavirus type 1 (BPV-1) E7 localization. BPV-1 E7 was observed in the cytoplasm of basal and lower spinous epithelial cells, coexpressed in the cytoplasm of basal cells with the E5 oncoprotein. E7 was also observed in nucleoli throughout the b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.513-521.2001
更新日期:2001-01-01 00:00:00
abstract::In humans, homologous to the E6-AP carboxyl terminus (HECT) and regulator of chromosome condensation 1 (RCC1)-like domain-containing protein 5 (HERC5) is an interferon-induced protein that inhibits replication of evolutionarily diverse viruses, including human immunodeficiency virus type 1 (HIV-1). To better understan...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00528-18
更新日期:2018-06-13 00:00:00
abstract::The promoter of the murine thymidine kinase gene contains a binding site for transcription factor E2F. Using cell lines (3T3-LT) conditionally expressing polyomavirus large T antigen from a hormone-responsive promoter and reporter gene constructs carrying the thymidine kinase promoter with intact or mutated E2F sites,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.4.1765-1771.1993
更新日期:1993-04-01 00:00:00
abstract::JC virus (JCV), along with other members of the polyomavirus family, encodes a class of highly conserved proteins, T antigens, that are capable of inducing aneuploidy in cultured cells. We have previously isolated T-antigen DNA variants of JCV from both colon cancer tissues and the corresponding nonneoplastic gastroin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.4.1996-2001.2001
更新日期:2001-02-01 00:00:00
abstract::Reverse genetic analyses of negative-strand RNA (NSR) viruses have provided enormous advances in our understanding of animal viruses over the past 20 years, but technical difficulties have hampered application to plant NSR viruses. To develop a reverse genetic approach for analysis of plant NSR viruses, we have engine...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01397-13
更新日期:2013-10-01 00:00:00
abstract::Human T-cell leukemia virus (HTLV) Tax protein has been implicated in the HTLV oncogenic process, primarily due to its pleiotropic effects on cellular genes involved in growth regulation and cell cycle control. To date, several approaches attempting to correlate Tax activation of the CREB/activating transcription fact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2655-2662.2000
更新日期:2000-03-01 00:00:00
abstract::A high prevalence (42.6%) of hepatitis B virus (HBV) infection was suspected in 195 formerly captive orangutans due to a large number of serum samples which cross-reacted with human HBV antigens. It was assumed that such viral infections were contracted from humans during captivity. However, two wild orangutans were i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7860-7865.1999
更新日期:1999-09-01 00:00:00
abstract::Increasing evidence suggests that the generation of cytotoxic T-lymphocyte (CTL) responses specific for a diversity of viral epitopes will be needed for an effective human immunodeficiency virus type 1 (HIV-1) vaccine. Here, we determine the frequencies of CTL responses specific for the simian immunodeficiency virus G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.5.2462-2467.2001
更新日期:2001-03-01 00:00:00
abstract::Some of the properties of a new human papovavirus, BK, have been examined. Host range studies of BK virus (BKV) showed human cells to be more sensitive to infection than monkey cells; human fetal brain cells appear to be highly sensitive to BKV, with the production of extensive cytopathology characterized by cytoplasm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.3.625-631.1973
更新日期:1973-09-01 00:00:00
abstract::Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8522-8530.1997
更新日期:1997-11-01 00:00:00
abstract::To assess the effect of the polyomavirus (Py) early proteins, the large T (LT), middle T (MT), and small T (ST) antigens, on gene expression from the Py late promoter, replication-defective plasmid constructs with the bacterial chloramphenicol acetyltransferase (cat) gene linked to this promoter were cotransfected int...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.1.275-285.1986
更新日期:1986-10-01 00:00:00
abstract::Evidence is presented that, in frog virus 3-infected BHK cells, inhibition of ribonucleic acid (RNA) synthesis is paralleled by a decreased activity of host cell RNA polymerase II, while the template ability of host cell nuclei and chromatin is unaffected. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.9.4.698-700.1972
更新日期:1972-04-01 00:00:00
abstract::Two major outer envelope glycoproteins of Epstein-Barr virus, gp350 and gp220, are known to be encoded by 3.2- and 2.5-kilobase RNAs which map to the same DNA fragment (M. Hummel, D. Thorley-Lawson, and E. Kieff, J. Virol. 49:413-417). These RNAs have the same 5' and 3' ends. The larger RNA is encoded by a 2,777-base ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.3.665-674.1985
更新日期:1985-06-01 00:00:00
abstract::ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.22.10658-10669.2000
更新日期:2000-11-01 00:00:00
abstract::The retrovirus SL3 induces T-cell lymphomas in mice. The transcriptional enhancer in the long terminal repeat (LTR) of SL3 contains two 72-bp repeats. Each repeat contains a binding site for the transcription factor CBF (also called AML1). The CBF binding sites are called core elements. SAA is a mutant that is identic...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.9.7599-7606.1999
更新日期:1999-09-01 00:00:00
abstract::Antibodies directed to Sindbis virus (SV) envelope protein E2 are able to control virus replication in vivo and in persistently infected cultures of neurons in vitro. We investigated the mechanisms by which anti-E2 monoclonal antibody (MAb) alters virus replication by using AT3 rat prostatic carcinoma cells expressing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7006-7014.1995
更新日期:1995-11-01 00:00:00