Abstract:
:JC virus (JCV), along with other members of the polyomavirus family, encodes a class of highly conserved proteins, T antigens, that are capable of inducing aneuploidy in cultured cells. We have previously isolated T-antigen DNA variants of JCV from both colon cancer tissues and the corresponding nonneoplastic gastrointestinal tissues, raising new questions about the role of JCV in the development of chromosomal instability of the colon. Based on the sequence of the transcriptional control region (TCR), JCV can be classified as archetype or tandem repeat variants. Among the latter, Mad-1, the prototype virus first isolated from a patient with progressive multifocal leukoencephalopathy, is characterized by lacking the 23- and 66-bp sequences that are present in the archetype and by duplication of a 98-bp sequence. In this study, we evaluated differences in the TCR of JCV isolated from colon cancer tissues and nonneoplastic epithelium. To characterize JCV variants, we first treated eight pairs of DNA samples from colon cancers and noncancerous tissue with topoisomerase I and then amplified and cloned the JCV TCR. We obtained 285 recombinant clones from the JCV TCR, 157 from nonneoplastic samples, and 128 from colon cancer tissues. Of these clones, 262 spanned the length of the JCV Mad-1 TCR: 99.3% from nonneoplastic samples and 82.8% from colon cancer tissues. In sequencing 54 clones in both directions, we did not find archetype JCV either in the nonneoplastic tissue or in the cancer samples. From all colon cancer tissues, 18 clones had a deletion of one 98-bp tandem repeat. This deleted strain was not detected in any of the nonneoplastic tissues (14 versus 0% [chi(2) = 23.6; P < 0.001]). Our study demonstrates that the only JCV strain present in the human colon is Mad-1, and the variant with a single 98-bp sequence is found exclusively in the cancer tissues. This strain may be involved in the development of chromosomal instability.
journal_name
J Viroljournal_title
Journal of virologyauthors
Ricciardiello L,Chang DK,Laghi L,Goel A,Chang CL,Boland CRdoi
10.1128/JVI.75.4.1996-2001.2001subject
Has Abstractpub_date
2001-02-01 00:00:00pages
1996-2001issue
4eissn
0022-538Xissn
1098-5514journal_volume
75pub_type
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journal_title:Journal of virology
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doi:10.1128/JVI.9.6.938-945.1972
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.3.1707-1711.1993
更新日期:1993-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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更新日期:2007-11-01 00:00:00
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更新日期:2007-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.3.776-787.1984
更新日期:1984-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章,收录出版
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更新日期:2011-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2895-2900.1990
更新日期:1990-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.12.7309-7318.1992
更新日期:1992-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00