Abstract:
:Mice infected with myopathic coxsackievirus B1 Tucson (CVB1(T)) develop chronic inflammatory myopathy (CIM) consisting of hind limb weakness and inflammation. Amyopathic virus variants are infectious but attenuated for CIM. In this report, viral clones, chimeras, and sequencing were used to identify viral determinants of CIM. Chimeras identified several regions involved in CIM and localized a weakness determinant to nucleotides 2493 to 3200 of VP1. Sequencing of multiple clones and viruses identified five candidate determinants that were strictly conserved in myopathic viruses with one located in the 5' untranslated region (UTR), three in the VP1 capsid, and one in the 3C protease. Taken together, these studies implicate Tyr-87 and/or Val-136 as candidate determinants of weakness. They also indicate that there are at least two determinants of inflammation and one additional determinant of weakness encoded by myopathic CVB1(T).
journal_name
J Viroljournal_title
Journal of virologyauthors
Tam PE,Weber-Sanders ML,Messner RPdoi
10.1128/jvi.77.21.11849-11854.2003subject
Has Abstractpub_date
2003-11-01 00:00:00pages
11849-54issue
21eissn
0022-538Xissn
1098-5514journal_volume
77pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.68.6.3943-3954.1994
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.5266-5270.2002
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.4.1448-1451.1988
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journal_title:Journal of virology
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journal_title:Journal of virology
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doi:10.1128/JVI.61.10.3082-3088.1987
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journal_title:Journal of virology
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journal_title:Journal of virology
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.49.2.319-324.1984
更新日期:1984-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.73.2.1186-1194.1999
更新日期:1999-02-01 00:00:00