Abstract:
:The in vitro transcription of the genome segments of insect cytoplasmic polyhedrosis virus was examined for a possible mechanism of coordinate control. Initiation of the first transcription cycle, primed by preincubation in the absence of CTP, was synchronous for all 10 viral genome segments. The time required for the completion of each genome transcript was proportional to the chain length, and all 10 mRNA's were synthesized in molar amounts inversely proportional to their molecular weights. The rate of chain elongation for all mRNA's was approximately 6 nucleotides per s. The combined results indicate that each of the viral genome segments is transcribed independently and suggest that RNA chain elongation, not initiation, is rate limiting for cytoplasmic polyhedrosis virus mRNA synthesis in vitro.
journal_name
J Viroljournal_title
Journal of virologyauthors
Smith RE,Furuichi Ydoi
10.1128/JVI.41.1.326-329.1982subject
Has Abstractpub_date
1982-01-01 00:00:00pages
326-9issue
1eissn
0022-538Xissn
1098-5514journal_volume
41pub_type
杂志文章abstract::In previous studies, we demonstrated that single-chain variable fragments (scFvs) from anti-human immunodeficiency virus (HIV) Env monoclonal antibodies act as entry inhibitors when tethered to the surface of target cells by a glycosyl-phosphatidylinositol (GPI) anchor. Interestingly, even if a virus escapes inhibitio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02080-17
更新日期:2018-03-14 00:00:00
abstract::Drug susceptibility and mutations in the reverse transcriptase (RT) gene were analyzed with 167 virus isolates from 38 patients treated with nevirapine, a potent nonnucleoside inhibitor of human immunodeficiency virus type 1 (HIV-1) RT. Resistant isolates emerged quickly and uniformly in all patients administered nevi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1660-1666.1994
更新日期:1994-03-01 00:00:00
abstract::Positional homologs to the UL51 open reading frame of herpes simplex virus type 1 have been identified throughout the herpesvirus family. However, no respective protein has so far been described for any of the herpesviruses. With rabbit antisera directed against oligopeptides predicted to comprise antigenic regions of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.7.5635-5638.1997
更新日期:1997-07-01 00:00:00
abstract::Our previous studies with influenza A viruses indicated that the association of M1 with viral RNA and nucleoprotein (NP) is required for the efficient formation of helical ribonucleoprotein (RNP) and for the nuclear export of RNPs. RNA-binding domains of M1 map to the following two independent regions: a zinc finger m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.18.9585-9591.2004
更新日期:2004-09-01 00:00:00
abstract::The family of giant viruses is still expanding, and evidence of a translational machinery is emerging in the virosphere. The Klosneuvirinae group of giant viruses was first reconstructed from in silico studies, and then a unique member was isolated, Bodo saltans virus. Here we describe the isolation of a new member in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01534-19
更新日期:2019-12-12 00:00:00
abstract::The inactivation of type C retroviruses by human serum may be a considerable impediment to the use of retroviral vectors in vivo for gene therapy. Here we show that virus inactivation is dependent both on the virus and on the cell line used to produce the virus. All viruses produced from murine NIH 3T3 or dog Cf2ThS+L...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.12.8001-8007.1994
更新日期:1994-12-01 00:00:00
abstract::High-risk human papillomavirus type 16 (HPV16) is the primary causative agent of cervical cancer and therefore is responsible for significant morbidity and mortality worldwide. Cellular transformation is mediated directly by the expression of viral oncogenes, the least characterized of which, E5, subverts cellular pro...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06243-11
更新日期:2012-05-01 00:00:00
abstract::The inbred mouse strain RIII has long been known for shedding large amounts of mouse mammary tumor virus (MMTV) particles in milk and for the development of hormone-dependent early mammary tumors at a very high incidence (>90%). We have established one RIII subline (RIII/Sa) that shows a pattern of virus expression an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.2.1055-1062.2004
更新日期:2004-01-01 00:00:00
abstract::Inhibition of RNA synthesis of the LH strain of type 3 poliovirus at the restrictive temperature occurs concurrently with the breakdown of noncapsid virus-specific peptide 2. This finding, along with other reported data (B.D. Korant, In Proteases and Biological Control, p. 621-644, 1975), suggests that noncapsid virus...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.27.2.443-448.1978
更新日期:1978-08-01 00:00:00
abstract::The multiplicity of attachment (MOA) of a virion in any particular time interval is the average number of cellular attachment opportunities that must be blocked to keep the virion in suspension. MOA is usually proportional to incubation time and cell concentration. Low MOA (like low multiplicity of infection) is requi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.3.1782-1789.1994
更新日期:1994-03-01 00:00:00
abstract::West Nile virus (WNV) replicates in the skin; however, cell targets in the skin have not been identified. In the current studies, WNV infected the epidermis and adnexal glands of mouse skin, and the epidermal cells were identified as keratinocytes by double labeling for WNV antigen and keratin 10. Inoculation of mice ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02692-10
更新日期:2011-05-01 00:00:00
abstract::HLA-B*52:01 is strongly associated with protection against HIV disease progression. However, the mechanisms of HLA-B*52:01-mediated immune control have not been well studied. We here describe a cohort with a majority of HIV C-clade-infected individuals from Delhi, India, where HLA-B*52:01 is highly prevalent (phenotyp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02025-19
更新日期:2020-06-16 00:00:00
abstract::Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02072-05
更新日期:2006-11-01 00:00:00
abstract::The two canine adenoviruses, infectious canine hepatitis (ICH) virus and infectious canine laryngotracheitis (ICL) virus (also designated as Toronto A26/61 virus), were studied with respect to their morphology and the biological properties of their soluble components. The two viruses were found to be composed of solub...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.4.507-512.1970
更新日期:1970-04-01 00:00:00
abstract::Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activati...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01559-07
更新日期:2008-01-01 00:00:00
abstract::Noroviruses account for 96% of viral gastroenteritis cases worldwide, with GII.4 strains responsible >80% of norovirus outbreaks. Histo-blood group antigens (HBGAs) are norovirus binding ligands, and antigenic and preferential HBGA binding profiles vary over time as new GII.4 strains emerge. The capsid P2 subdomain fa...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.06189-11
更新日期:2012-01-01 00:00:00
abstract::H5N1 highly pathogenic avian influenza virus (HPAIV) has continued to spread and poses a significant threat to both animal and human health. Current influenza vaccine strategies have limitations that prevent their effective use for widespread inoculation of animals in the field. Vaccine strains of Newcastle disease vi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01514-06
更新日期:2007-01-01 00:00:00
abstract::Severe complications of Zika virus (ZIKV) infection might be caused by inflammation, but how ZIKV induces proinflammatory cytokines is not understood. In this study, we show opposite regulatory effects of the ZIKV NS5 protein on interferon (IFN) signaling. Whereas ZIKV and its NS5 protein were potent suppressors of ty...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00163-17
更新日期:2017-06-26 00:00:00
abstract:UNLABELLED:Noroviruses are the leading cause of acute gastroenteritis outbreaks worldwide. The majority of norovirus outbreaks are caused by genogroup II.4 (GII.4). Novel GII.4 strains emerge every 2 to 4 years and replace older variants as the dominant norovirus. Novel variants emerge through a combination of recombin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02494-14
更新日期:2014-12-01 00:00:00
abstract::Newcastle disease virus (NDV) belongs to serotype 1 of the avian paramyxoviruses (APMV-1) and causes severe disease in chickens. Current live attenuated NDV vaccines are not fully satisfactory. An alternative is to use a viral vector vaccine that infects chickens but does not cause disease. APMV serotype 3 infects a w...
journal_title:Journal of virology
pub_type: 杂志文章,收录出版
doi:10.1128/JVI.00367-11
更新日期:2011-07-01 00:00:00
abstract::The polypeptides of three paramyxoviruses (simian virus 5, Newcastle disease virus, and Sendai virus) were separated by polyacrylamide gel electrophoresis. Glycoproteins were identified by the use of radioactive glucosamine as a carbohydrate precursor. The protein patterns reveal similarities among the three viruses. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.1.47-52.1971
更新日期:1971-01-01 00:00:00
abstract::Dendritic cells (DC) are believed to play an important role in the initiation of innate and adaptive immune responses to infection, including respiratory tract infections, where respiratory DC (RDC) perform this role. In this report, we examined the susceptibilities of isolated murine RDC to influenza virus infection ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02367-07
更新日期:2008-05-01 00:00:00
abstract::We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082-3087, 1998). We now extend that analysis to show that additional passag...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.49-56.2000
更新日期:2000-01-01 00:00:00
abstract::The E5 oncoprotein of bovine papillomavirus type 1 is a 44-amino-acid, hydrophobic polypeptide which localizes predominantly in Golgi membranes and appears to transform cells through the activation of tyrosine kinase growth factor receptors. In fibroblasts, E5 interacts with both the 16-kilodalton vacuolar ATPase subu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.4.2420-2430.1996
更新日期:1996-04-01 00:00:00
abstract::Glycoprotein B (gB) is a conserved herpesvirus virion component implicated in membrane fusion. As with many-but not all-herpesviruses, the gB of murid herpesvirus 4 (MuHV-4) is cleaved into disulfide-linked subunits, apparently by furin. Preventing gB cleavage for some herpesviruses causes minor infection deficits in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00709-13
更新日期:2013-10-01 00:00:00
abstract::LA3382 is a temperature-sensitive replication-defective mutant of Rous sarcoma virus that contains four active mutations. In this study we performed experiments to determine the biochemical defect that blocks the synthesis of infections virus late in the replication cycle. At the nonpermissive temperature (41 degrees ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.3.752-761.1981
更新日期:1981-12-01 00:00:00
abstract::Transient lymphopenia is a hallmark of measles virus (MV)-induced immunosuppression. To address to what extent replenishment of the peripheral lymphocyte compartment from bone marrow (BM) progenitor/stem cells might be affected, we analyzed the interaction of wild-type MV with hematopoietic stem and progenitor cells (...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00532-11
更新日期:2011-08-01 00:00:00
abstract::Plasma human immunodeficiency virus type 1 (HIV-1) turnover and kinetics were studied in children aged 15 days to 2 years following the initiation of a triple antiretroviral drug regimen consisting of zidovudine, lamivudine, and nevirapine. HIV-1 turnover was at least as rapid as that previously described in adults; t...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.73.1.362-367.1999
更新日期:1999-01-01 00:00:00
abstract::Measles virus (MV) isolated in B95a cells, a marmoset B-cell line, retains full pathogenicity for cynomolgus monkeys, while its derivative obtained by adaptation to the growth in Vero cells, a monkey kidney cell line, loses the pathogenic potential (F. Kobune, H. Sakata, and A. Sugiura, J. Virol. 64:700-705, 1990). He...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.11.8690-8696.1998
更新日期:1998-11-01 00:00:00
abstract::The initial host response to viral infection occurs after Toll-like receptors (TLRs) on dendritic cells (DC) are stimulated by viral nucleic acids (double-stranded RNA, single-stranded RNA) and alpha interferon (IFN-alpha) and IFN-beta are produced. We hypothesized that pharmacologic induction of innate antiviral resp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.22.14355-14370.2005
更新日期:2005-11-01 00:00:00