Abstract:
:Glycoprotein B (gB) is a conserved herpesvirus virion component implicated in membrane fusion. As with many-but not all-herpesviruses, the gB of murid herpesvirus 4 (MuHV-4) is cleaved into disulfide-linked subunits, apparently by furin. Preventing gB cleavage for some herpesviruses causes minor infection deficits in vitro, but what the cleavage contributes to host colonization has been unclear. To address this, we mutated the furin cleavage site (R-R-K-R) of the MuHV-4 gB. Abolishing gB cleavage did not affect its expression levels, glycosylation, or antigenic conformation. In vitro, mutant viruses entered fibroblasts and epithelial cells normally but had a significant entry deficit in myeloid cells such as macrophages and bone marrow-derived dendritic cells. The deficit in myeloid cells was not due to reduced virion binding or endocytosis, suggesting that gB cleavage promotes infection at a postendocytic entry step, presumably viral membrane fusion. In vivo, viruses lacking gB cleavage showed reduced lytic spread in the lungs. Alveolar epithelial cell infection was normal, but alveolar macrophage infection was significantly reduced. Normal long-term latency in lymphoid tissue was established nonetheless.
journal_name
J Viroljournal_title
Journal of virologyauthors
Glauser DL,Milho R,Frederico B,May JS,Kratz AS,Gillet L,Stevenson PGdoi
10.1128/JVI.00709-13subject
Has Abstractpub_date
2013-10-01 00:00:00pages
10828-42issue
19eissn
0022-538Xissn
1098-5514pii
JVI.00709-13journal_volume
87pub_type
杂志文章abstract::Utilizing a human cytomegalovirus-specific fusion inhibitor and an antiglycoprotein H antibody, we studied the role of virion fusion in interferon-stimulated gene (ISG) induction. Our results indicate that ISG induction does not occur when virion-mediated, post-high-affinity attachment events are inhibited by either r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6688-6691.2004
更新日期:2004-06-01 00:00:00
abstract::We have selected neutralization escape mutants by using a monoclonal antibody (nt-MAb) against a sequential epitope between amino acids 93 through 104 (neutralization antigenic site I) of poliovirus type 1 Mahoney. The majority of mutants were also resistant against five strain-specific nt-MAbs which recognized confor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.5.1845-1848.1988
更新日期:1988-05-01 00:00:00
abstract::Herpes simplex virus 1 (HSV-1) is a common human pathogen that causes lifelong latent infection of sensory neurons. Non-nucleoside inhibitors that can limit HSV-1 recurrence are particularly useful in treating immunocompromised individuals or cases of emerging acyclovir-resistant strains of herpesvirus. We report that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01492-10
更新日期:2011-05-01 00:00:00
abstract::A number of options are available to modify and improve DNA vaccines. An interesting approach to improve DNA vaccines is to fuse bioactive domains, like cytotoxic-T-lymphocyte-associated protein 4 (CTLA-4), to an antigen. Such fusion antigens are expressed in vivo and directed to immune cells by the specific bioactive...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.10.6368-6376.2005
更新日期:2005-05-01 00:00:00
abstract::We measured the quantity of plasma feline immunodeficiency virus (FIV) RNA using a real-time sequence detecting system. Plasma viral RNA load was shown to correlate with the clinical stage, survival time, and disease progression in naturally FIV-infected cats. The present study indicates that the plasma viral RNA load...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.19.10079-10083.2002
更新日期:2002-10-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1)-encoded RNA-binding protein Tat is known to play an essential role in viral gene expression. In the search for novel compounds to inhibit Tat transactivity, one coumarin derivative, BPRHIV001, was identified, with a 50% effective concentration (EC(50)) against HIV-1 at 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00175-11
更新日期:2011-09-01 00:00:00
abstract::The unit-length genome of human herpesvirus 6 (HHV-6) consists of a single unique component (U) bounded by direct repeats DRL and DRR and forms head-to-tail concatemers during productive infection. cis-elements which mediate cleavage and packaging of progeny virions (a sequences) are found at the termini of all herpes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.5.3007-3014.1994
更新日期:1994-05-01 00:00:00
abstract::A study of virus-specific protein synthesis in infectious pancreatic necrosis virus-infected RTG-2 cells was undertaken to find a relationship between the coding capacity of virus genome (two segments of double-stranded RNA of 2.5 X10(6) and 2.3 X 10(6) molecular weight) and the sizes and relative amounts of polypepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.1.242-258.1977
更新日期:1977-01-01 00:00:00
abstract::BCX 2798 (4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid) effectively inhibited the activities of the hemagglutinin-neuraminidase (HN) of human parainfluenza viruses (hPIV) in vitro and protected mice from lethal infection with a recombinant Sendai virus whose HN ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00474-08
更新日期:2008-09-01 00:00:00
abstract::Simian virus 40 (SV40) large T antigen (SVT) interferes with normal cell regulation and thus has been used to identify cellular components controlling proliferation and homeostasis. We have previously shown that SVT-mediated transformation requires interaction with the histone acetyltransferases (HATs) CBP/p300 and no...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02658-13
更新日期:2013-12-01 00:00:00
abstract:UNLABELLED:Since the 1960s, simian hemorrhagic fever virus (SHFV; Nidovirales, Arteriviridae) has caused highly fatal outbreaks of viral hemorrhagic fever in captive Asian macaque colonies. However, the source(s) of these outbreaks and the natural reservoir(s) of this virus remain obscure. Here we report the identifica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02203-14
更新日期:2014-11-01 00:00:00
abstract::Information was sought on the temporal distribution of transmissible mink encephalopathy virus in royal pastel mink inoculated subcutaneously with 10(3.0) 50% intracerebral lethal doses of the Idaho strain. As determined by intracerebral assay in mink, extremely little replication of the virus occurred during the prec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.10.3235-3240.1987
更新日期:1987-10-01 00:00:00
abstract::Long interspersed nuclear element 1 (LINE-1; L1) retrotransposons are the most common retroelements in mammalian genomes. Unlike individual families of endogenous retroviruses (ERVs), they have remained active throughout the mammalian radiation and are responsible for most of the retroelement movement and much genome ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05180-11
更新日期:2011-12-01 00:00:00
abstract::Highly pathogenic H5N1 influenza A virus remains a substantial threat to public health. To understand the molecular basis and host mechanism for the high virulence of H5N1 viruses in mammals, we compared two H5N1 isolates which have similar genetic backgrounds but greatly differ in their virulence in mice. A/Chicken/J...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02891-12
更新日期:2013-03-01 00:00:00
abstract::This study on Tomato bushy stunt virus identified and defined three previously unknown regulatory sequences involved in RNA accumulation that are located within the 3'-proximal nested movement protein genes p22 and p19. The first is a 16-nucleotide (nt) element termed III-A that is positioned at the very 3' end of p22...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.12747-12757.2002
更新日期:2002-12-01 00:00:00
abstract::Lassa fever (LF) is a potentially lethal human disease that is caused by the arenavirus Lassa virus (LASV). Annually, around 300,000 infections with up to 10,000 deaths occur in regions of Lassa fever endemicity in West Africa. Here we demonstrate that mice lacking a functional STAT1 pathway are highly susceptible to ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01433-13
更新日期:2013-10-01 00:00:00
abstract::Parvovirus capsids are small but complex molecular machines responsible for undertaking many of the steps of cell infection, genome packing, and cell-to-cell as well as host-to-host transfer. The details of parvovirus infection of cells are still not fully understood, but the processes must involve small changes in th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01871-16
更新日期:2017-01-03 00:00:00
abstract::The putative role of nitric oxide in the neuropathogenesis of Borna disease was investigated by determining changes in the expression of inducible nitric oxide synthase (iNOS) mRNA and constitutively expressed NOS (cNOS) mRNA in brains of Borna disease virus (BDV)-infected rats. iNOS mRNA was not detected in normal ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5786-5791.1993
更新日期:1993-10-01 00:00:00
abstract::An E1-E3-deleted recombinant adenovirus vector expressing the hybrid protein TetR-KRAB has been produced. In this virus, AdTG9562, the E2 transcription is regulated by TetR-KRAB and tetO sequences inserted in cis. In the absence of tetracycline, a strong reduction in E2A gene expression, viral DNA replication, and lat...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.4.3307-3311.1997
更新日期:1997-04-01 00:00:00
abstract::The nonpathogenic human adeno-associated virus (AAV) has developed a mechanism to integrate its genome into human chromosome 19 at 19q13.4 (termed AAVS1), thereby establishing latency. Here, we provide evidence that the chromosomal signals required for site-specific integration are conserved in the mouse genome proxim...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8917-8921.2004
更新日期:2004-08-01 00:00:00
abstract::Infectious vaccinia virus can be purified from whole cells by experimentally induced lysis (intracellular virus) or from supernatant growth medium (extracellular virus). Extracellular virus and intracellular virus differed by buoyant density (1.237 versus 1.272 g/cm3), phospholipid content and composition, and polypep...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.903-913.1981
更新日期:1981-09-01 00:00:00
abstract::The emergence of antiretroviral (ARV) drug-resistant human immunodeficiency virus type 1 (HIV-1) quasispecies is a major cause of treatment failure. These variants are usually replaced by drug-sensitive ones when the selective pressure of the drugs is removed, as the former have reduced fitness in a drug-free environm...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.14.7000-7009.2002
更新日期:2002-07-01 00:00:00
abstract::The copy choice model for the generation of defective interfering (DI) particles of vesicular stomatitis virus suggests that during replication the polymerase prematurely terminates, moves with the nascent daughter strand to another site on the same or a different template molecule, and resumes elongation of the nasce...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.2.515-521.1984
更新日期:1984-08-01 00:00:00
abstract::African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs that has significant economic consequences for the swine industry. The control of African swine fever (ASF) has been hampered by the unavailability of vaccines. Successful experimental vaccines hav...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01760-16
更新日期:2016-12-16 00:00:00
abstract::The human papillomavirus (HPV) E6 oncoproteins recruit the cellular ubiquitin ligase E6AP/UBE3A to target cellular substrates for proteasome-mediated degradation, and one consequence of this activity is the E6 stimulation of E6AP autoubiquitination and degradation. Recent studies identified an autism-linked mutation w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01390-17
更新日期:2017-10-27 00:00:00
abstract::The steady-state level and metabolic half-life of retinoblastoma tumor suppressor protein pRB are decreased in cells that express high-risk human papillomavirus (HPV) E7 proteins. Here we show that pRB degradation is a direct activity of E7 and does not reflect a property of cell lines acquired during the selection pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.16.7583-7591.2001
更新日期:2001-08-01 00:00:00
abstract::The assembly of hepatitis C virus (HCV) is poorly understood, largely due to the lack of mammalian cell culture systems that are easily manipulated and produce high titers of virus. This problem is highlighted by the inability of the recently established HCV replicon systems to support HCV capsid assembly despite high...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.17.9257-9269.2004
更新日期:2004-09-01 00:00:00
abstract::Previous studies have suggested that the U(L)17 gene of herpes simplex virus type 1 (HSV-1) is essential for virus replication. In this study, viral mutants incorporating either a lacZ expression cassette in place of 1,490 bp of the 2,109-bp U(L)17 open reading frame [HSV-1(deltaU(L)17)] or a DNA oligomer containing a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.3779-3788.1998
更新日期:1998-05-01 00:00:00
abstract::Of the four required herpes simplex virus (HSV) entry glycoproteins, the precise role of gH-gL in fusion remains the most elusive. The heterodimer gH-gL has been proposed to mediate hemifusion after the interaction of another required glycoprotein, gD, with a receptor. To identify functional domains of HSV-1 gH, we ge...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02215-09
更新日期:2010-02-01 00:00:00
abstract::The recently discovered hepatitis G virus (HGV) or GB virus C (GBV-C) is widely distributed in human populations, and homologues such as HGV/GBV-CCPZ and GBV-A are found in a variety of different primate species. Both epidemiological and phylogenetic analyses support the hypothesis that GB viruses coevolved with their...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5787-5794.1999
更新日期:1999-07-01 00:00:00