Abstract:
:Infectious vaccinia virus can be purified from whole cells by experimentally induced lysis (intracellular virus) or from supernatant growth medium (extracellular virus). Extracellular virus and intracellular virus differed by buoyant density (1.237 versus 1.272 g/cm3), phospholipid content and composition, and polypeptide pattern. Differences in structural polypeptides on the virus surface could be detected by lactoperoxidase-catalyzed radioiodination or Brij treatment. Characteristic of extracellular virus was an additional polypeptide, with a molecular weight of 37,000 (37K), which represented 5 to 7% of the total particle protein. Antibodies to the 37K protein detected only some of the cell-associated particles late in normal infection. Upon treatment of infected cultures with N1-isonicotinoyl-N2-3-methyl-4-chlorobenzoylhydrazine, a drug which prevents vaccinia virus release, no particle-associated 37K protein could be detected. In all other properties tested so far, except for a slight difference in phospholipid composition, the virus obtained in the presence of the drug resembled the normal intracellular virus. N1-Isonicotinoyl-N2-3-methyl-4-chlorobenzoylhydrazine prevented vesicularization of intracellular viral particles. Lack of vesicularization was accompanied by the absence of particle-associated 37K viral protein and seemed to correlate with an inhibition of virus dissemination to the cell periphery.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hiller G,Eibl H,Weber Kdoi
10.1128/JVI.39.3.903-913.1981subject
Has Abstractpub_date
1981-09-01 00:00:00pages
903-13issue
3eissn
0022-538Xissn
1098-5514journal_volume
39pub_type
杂志文章abstract::Lentiviruses are nononcogenic retroviruses that cause persistent infections and slowly progressive diseases. Visna virus, a lentivirus of sheep, persists in cells of the macrophage lineage despite the presence of neutralizing antibodies in the animal. These antibodies are measured by prevention of virus replication in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.59.1.37-44.1986
更新日期:1986-07-01 00:00:00
abstract::Persistent infection with hepatitis C virus (HCV) is a major cause of chronic liver diseases. The aim of this study was to identify host cell factor(s) participating in the HCV replication complex (RC) and to clarify the regulatory mechanisms of viral genome replication dependent on the host-derived factor(s) identifi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02179-08
更新日期:2009-05-01 00:00:00
abstract::The internal structural proteins of retroviruses are proteolytically processed from the Gag polyprotein, which alone is able to assemble into virus-like particles when expressed in cells. All Gag proteins contain domains corresponding to the three structural proteins MA, CA, and NC. We have expressed the CA and NC dom...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.10.6487-6497.1995
更新日期:1995-10-01 00:00:00
abstract::Artificial target sequences for tissue-specific miRNAs have recently been introduced as a new means for altering the tissue tropism of viral replication. This approach can be used to improve the safety of oncolytic viruses for cancer virotherapy by restricting their replication in unwanted tissues, such as the liver. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01940-12
更新日期:2013-01-01 00:00:00
abstract::The bovine papillomavirus E5 gene encodes a 44-amino-acid, homodimeric transmembrane protein that is the smallest known transforming protein. The E5 protein transforms cultured fibroblasts by forming a stable complex with the endogenous platelet-derived growth factor (PDGF) beta receptor through transmembrane and juxt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.4.3264-3272.1999
更新日期:1999-04-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) vectors that express heterologous antigens have shown promise as vaccines in preclinical studies. The efficacy of VSV-based vaccines can be improved by engineering vectors that enhance innate immune responses. We previously generated a VSV vaccine vector that incorporates two enhancing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02898-13
更新日期:2014-01-01 00:00:00
abstract::Intrarectal simian immunodeficiency virus (SIV) infection in rhesus macaques is a model for sexual transmission of primate retroviruses. Phylogenetic studies on envelope gene sequences that were present in blood following intrarectal SIV inoculation provided evidence for selective amplification of a subset of viruses ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.10.6876-6883.1996
更新日期:1996-10-01 00:00:00
abstract::The assembly of human cytomegalovirus (HCMV) is thought to be similar to that which has been proposed for alphaherpesviruses and involve envelopment of tegumented subviral particles at the nuclear membrane followed by export from the cell by a poorly defined pathway. However, several studies have shown that at least t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.975-986.2000
更新日期:2000-01-01 00:00:00
abstract::The E2 gene products of papillomavirus play key roles in viral replication, both as regulators of viral transcription and as auxiliary factors that act with E1 in viral DNA replication. We have carried out a detailed structure-function analysis of conserved amino acids within the N-terminal domain of the human papillo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.3.1602-1611.1996
更新日期:1996-03-01 00:00:00
abstract::An extensive genetic map of coliphage P1 has been constructed for 113 amber mutants, using primarily a modification of the conventional complementation spot test. These spot tests failed to classify the mutants into cistrons, but when they were quantitated they permitted assignment of the mutants into 10 linkage clust...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.20.1.177-187.1976
更新日期:1976-10-01 00:00:00
abstract::To examine the cell fusion activity of hepatitis C virus (HCV) envelope proteins (E1 and E2), we have established a sensitive cell fusion assay based on the activation of a reporter gene as described previously (O. Nussbaum, C. C. Broder, and E. A. Berger, J. Virol. 68:5411-5422, 1994). The chimeric HCV E1 and E2 prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.11.5066-5074.2000
更新日期:2000-06-01 00:00:00
abstract::Vaccine-induced memory is necessary for protective immunity to pathogens, but many viruses induce a state of transient immune suppression that might contribute to the inability of a vaccine to elicit immunity. We evaluated here the fate of bystander T cells activated by third party cognate antigens during acute viral ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02516-10
更新日期:2011-06-01 00:00:00
abstract::The Ebola filoviruses are aggressive pathogens that cause severe and often lethal hemorrhagic fever syndromes in humans and nonhuman primates. To date, no effective therapies have been identified. To analyze the entry and fusion properties of Ebola virus, we adapted a human immunodeficiency virus type 1 (HIV-1) virion...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.918-926.2005
更新日期:2005-01-01 00:00:00
abstract::Influenza A virus matrix protein M1 is involved in multiple stages of the viral infectious cycle. Despite its functional importance, our present understanding of this essential viral protein is limited. The roles of a small subset of specific amino acids have been reported, but a more comprehensive understanding of th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00161-19
更新日期:2019-06-14 00:00:00
abstract::To date, no vaccine that is safe and effective against herpes simplex virus 2 (HSV-2) disease has been licensed. In this study, we evaluated a DNA prime-formalin-inactivated-HSV-2 (FI-HSV2) boost vaccine approach in the guinea pig model of acute and recurrent HSV-2 genital disease. Five groups of guinea pigs were immu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02521-10
更新日期:2011-04-01 00:00:00
abstract::Xenotransplantation of porcine organs carries the risk of reactivation of latent virus in donor and recipient tissues as well as transmission of viruses between species. We have investigated the activation of baboon cytomegalovirus (BCMV) and porcine CMV (PCMV) in a pig-to-primate model of xenotransplantation. Tissues...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4734-4740.2002
更新日期:2002-05-01 00:00:00
abstract::Human immunodeficiency virus type 1 contains a transmembrane glycoprotein with an unusually long cytoplasmic domain. To determine the role of this domain in virus replication, a series of single nucleotide changes that result in the insertion of premature termination codons throughout the cytoplasmic domain has been c...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6616-6625.1992
更新日期:1992-11-01 00:00:00
abstract::Bacteriophage phi105 is a temperate bacteriophage for Bacillus subtilis 168. Temperature-sensitive and plaque mutants of phi105 were isolated. The results of two- and three-factor crosses with these mutants suggest the vegetative map of phi105 to be circular. The location of prophage phi105 between bacterial markers p...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.1.38-44.1969
更新日期:1969-01-01 00:00:00
abstract::We report the discovery and analysis of an endogenous foamy virus (PSFVaye) within the genome of the aye-aye (Daubentonia madagascariensis), a strepsirrhine primate from Madagascar. Phylogenetic analyses indicate that PSFVaye is divergent from all known simian foamy viruses, suggesting an association between foamy vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00650-12
更新日期:2012-07-01 00:00:00
abstract::Multimerization of the Hantaan virus nucleocapsid protein (NP) in Hantaan virus-infected Vero E6 cells was observed in a competitive enzyme-linked immunosorbent assay (ELISA). Recombinant and truncated NPs of Hantaan, Seoul, and Dobrava viruses lacking the N-terminal 49 amino acids were also detected as multimers. Alt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.943-952.2003
更新日期:2003-01-01 00:00:00
abstract::Cholesterol has been implicated in various viral life cycle steps for different enveloped viruses, including viral entry into host cells, cell-cell fusion, and viral budding from infected cells. Enveloped viruses acquire their membranes from their host cells. Though cholesterol has been associated with binding and ent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02323-20
更新日期:2021-01-06 00:00:00
abstract::Homologous recombination is an important biological process that facilitates genome rearrangement and repair of DNA double-strand breaks (DSBs). The induction of Epstein-Barr virus (EBV) lytic replication induces ataxia telangiectasia-mutated (ATM)-dependent DNA damage checkpoint signaling, leading to the clustering o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00049-09
更新日期:2009-07-01 00:00:00
abstract::The open reading frame III of Borna disease virus (BDV) codes for a protein with a mass of 16 kDa, named p16 or BDV-M. p16 was described as an N-glycosylated protein in several previous publications and therefore was termed gp18, although the amino acid sequence of p16 does not contain any regular consensus sequence f...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.24.12098-12104.2001
更新日期:2001-12-01 00:00:00
abstract::In most prion diseases, infectivity accumulates in lymphoreticular organs early after infection. Defects in hematopoietic compartments, such as impaired B-cell maturation, or in stromal compartments, such as abrogation of follicular dendritic cells, can delay or prevent lymphoreticular prion colonization. However, the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.7097-7106.2001
更新日期:2001-08-01 00:00:00
abstract::Kaposi's sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus that has been implicated in the pathogenesis of Kaposi's sarcoma. KSHV encodes K-bZIP (open reading frame K8), a protein that belongs to the basic region-leucine zipper (bZIP) family of transcription factors. Here we show that K-bZIP associates with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11977-11982.2000
更新日期:2000-12-01 00:00:00
abstract::The mutational alterations in polyoma virus mutants ts-a and ts-25E which cause their large T-antigens to be thermolabile have been identified. In ts-a, a G leads to A transition at nucleotide 2193 causes the replacement of Ala (GCT) by Thr (ACT). In ts-25E, a G leads to T transversion at nucleotide 2883 causes the re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.3.1094-1098.1981
更新日期:1981-03-01 00:00:00
abstract::The human polyomavirus JC virus (JCV) establishes persistent infections in most individuals and is the etiologic agent of progressive multifocal leukoencephalopathy. In this report, we describe the establishment of a soluble cell-free system that is capable of replicating exogenous plasmid DNA containing the JCV origi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7421-7428.1997
更新日期:1997-10-01 00:00:00
abstract::During dengue virus replication, an incomplete cleavage of the envelope glycoprotein prM, generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In this study, sequential immunoprecipitation and cryoelectron microscopy revealed a third type of extracellular particles, the partia...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00696-10
更新日期:2010-08-01 00:00:00
abstract::It has been proposed recently that the cell surface peptidase CD26 acts in concert with CD4, the human immunodeficiency virus (HIV) primary receptor molecule, to mediate HIV entry into permissive cells. We have failed to detect significant levels of CD26 cell surface expression and enzymatic activity in a number of co...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6535-6546.1994
更新日期:1994-10-01 00:00:00
abstract::Autographa californica nuclear polyhedrosis virus (AcMNPV) is a double-stranded DNA virus that expresses several immediate-early genes under the control of different promoters. The expression of one of these transcription units, IE-N, is shown here, by a transient expression assay, to be regulated by both cis- and tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.2.945-951.1991
更新日期:1991-02-01 00:00:00