Abstract:
:LA3382 is a temperature-sensitive replication-defective mutant of Rous sarcoma virus that contains four active mutations. In this study we performed experiments to determine the biochemical defect that blocks the synthesis of infections virus late in the replication cycle. At the nonpermissive temperature (41 degrees C) cells infected with LA3382 synthesized virus particles which were noninfectious and exhibited significant reductions in the amounts of gp85 and gp37 present in the virions. An analysis of the intracellular viral polypeptides indicated that the precursor of the viral glycoproteins (Pr95) were synthesized normally but underwent cleavage at a reduced rate at the restrictive temperature. Pr95 did not accumulate in infected cells ans was inserted into mutant virions at 41 degrees C; however, Pr95 was cleaved in such a way that gp85 was released from the viruses and could be detected in the supernatant medium by immunoprecipitation. The virus-free glycoprotein was indistinguishable from wild-type gp85 and may have been released due to an anomalous cleavage. Pulse-chase experiments also indicated that the Pr180 polyprotein precursor of the reverse transcriptase was cleaved to the active form of the enzyme more slowly at 41 degrees C in LA3382-infected cells. This accounted for the twofold lower level of polymerase activity found in mutant virions at 41 degrees C, defect which probably did not account for the observed 20- to 50-fold reduction in infectivity. Furthermore, the replication defect was not complemented by an env deletion mutant Rous sarcoma virus [RSV(-)[, which should complement a pol defect. Therefore, we conclude that the major lesion that impairs replication in LA3382 is within the env gene.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hardwick JM,Hunter Edoi
10.1128/JVI.40.3.752-761.1981subject
Has Abstractpub_date
1981-12-01 00:00:00pages
752-61issue
3eissn
0022-538Xissn
1098-5514journal_volume
40pub_type
杂志文章abstract::Natural evolution in primate lentiviral reverse transcriptase (RT) appears to have been constrained by the necessity to maintain function within an asymmetric protein composed of two identical primary amino acid sequences (66 kDa), of which one is cleaved (51 kDa). In this study, a detailed phylogenetic analysis now s...
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pub_type: 杂志文章
doi:10.1128/JVI.00991-10
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.57.1.328-334.1986
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pub_type: 杂志文章
doi:10.1128/JVI.75.19.9483-9492.2001
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JVI.71.9.7005-7011.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.8.4722-4731.1993
更新日期:1993-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.8.6559-6564.1998
更新日期:1998-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.43.3.772-777.1982
更新日期:1982-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.12.8645-8652.1996
更新日期:1996-12-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.14.2.349-365.1974
更新日期:1974-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7965-7973.1996
更新日期:1996-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.123-131.1991
更新日期:1991-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00842-17
更新日期:2017-09-27 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.2.725-731.2005
更新日期:2005-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.21.13497-13508.2005
更新日期:2005-11-01 00:00:00
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journal_title:Journal of virology
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更新日期:2013-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-09-29 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.11.4882-4889.1989
更新日期:1989-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-08-12 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00823-11
更新日期:2011-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.185-192.1983
更新日期:1983-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00943-12
更新日期:2012-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01476-18
更新日期:2018-12-10 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.1.207-218.1981
更新日期:1981-07-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.10.4816-4823.2000
更新日期:2000-05-01 00:00:00
abstract::Chromatography of RNA polymerase purified from vaccinia virions and from vaccinia virus-infected HeLa cells resulted in the separation of populations active for early and late transcription. An RNA polymerase population immunodepleted for the vaccinia virus H4 gene peptide could support late transcription. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.4.2602-2604.1995
更新日期:1995-04-01 00:00:00