Abstract:
:Reactivations of persistent viral infections pose a significant medical problem in immunocompromised cancer, transplant, and AIDS patients, yet little is known about how persistent viral infections are immunologically controlled. Here we describe a mouse model for investigating the role of the immune response in controlling a persistent retroviral infection. We demonstrate that, following recovery from acute Friend virus infection, a small number of B cells evade immunological destruction and harbor persistent virus. In vivo depletions of T-cell subsets in persistently infected mice revealed a critical role for CD4(+) T cells in controlling virus replication, spread to the erythroid lineage, and induction of erythroleukemia. The CD4(+) T-cell effect was independent of CD8(+) T cells and in some cases was also independent of virus-neutralizing antibody responses. Thus, the CD4(+) T cells may have had a direct antiviral effect. These results may have relevance for human immunodeficiency virus (HIV) infections where loss of CD4(+) T cells is associated with an increase in HIV replication, reactivation of persistent viruses, and a high incidence of virus-associated cancers.
journal_name
J Viroljournal_title
Journal of virologyauthors
Hasenkrug KJ,Brooks DM,Dittmer Udoi
10.1128/JVI.72.8.6559-6564.1998subject
Has Abstractpub_date
1998-08-01 00:00:00pages
6559-64issue
8eissn
0022-538Xissn
1098-5514journal_volume
72pub_type
杂志文章abstract::Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) transactivates EBV genes in latently infected B cells. We have shown that mitotic hyperphosphorylation of EBNA2 suppresses its ability to transactivate the latent membrane protein 1 (LMP1) promoter. In this follow-up study, we identify EBNA2 Ser243 as a phosphorylatio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.2045-2050.2006
更新日期:2006-02-01 00:00:00
abstract::Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane-associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.3.1438-1451.2005
更新日期:2005-02-01 00:00:00
abstract::In this study, we investigated the interaction of reovirus particles with cell membranes by using a 51Cr release assay. We confirmed prior observations (J. Borsa, B. D. Morash, M. D. Sargent, T. P. Copps, P. A. Lievaart, and J. G. Szekely, J. Gen. Virol. 45:161-170, 1979) that intermediate subviral particles (ISVPs) o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5339-5345.1993
更新日期:1993-09-01 00:00:00
abstract::Virus-specific RNA synthesized in monkey cells after infection by both wild-type simian virus 40 (SV40) and the early SV40 temperature-sensitive mutant tsA58 has been analyzed. The fraction of SV40-specific RNA increased throughout infection with either wild-type SV40 or with tsA58 in direct proportion to the accumula...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.23.1.167-176.1977
更新日期:1977-07-01 00:00:00
abstract::Most strains of human immunodeficiency virus type 1 (HIV-1) which have only been carried in vitro in peripheral blood mononuclear cells (primary isolates) can be neutralized by antibodies, but their sensitivity to neutralization varies considerably. To study the parameters that contribute to the differential neutraliz...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.6988-6996.1998
更新日期:1998-09-01 00:00:00
abstract::We previously established a genetic assay for retroviral Gag polyprotein multimerization (J. Luban, K. B. Alin, K. L. Bossolt, T. Humaran, and S. P. Goff, J. Virol. 66:5157-5160, 1992). Here we use this assay to demonstrate homomeric interactions between Gag polyproteins encoded by six different retroviruses. Of the G...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5300-5305.1994
更新日期:1994-08-01 00:00:00
abstract::The nucleotide sequence was determined for the hemagglutinin gene of the Hong Kong subtype influenza strain A/Bangkok/1/79. The amino acid sequence predicted from these data shows a total of 36 amino acid changes as compared with hemagglutinin for a 1968 Hong Kong strain, 11 more than had occurred in a 1975 strain. Th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.39.3.663-672.1981
更新日期:1981-09-01 00:00:00
abstract::Recruitment and extravasation of T cells through the blood-brain barrier are favored by adhesion molecule-mediated interactions of circulating T cells with endothelial cells. Since a common pathological finding in human T-cell leukemia virus type 1 (HTLV-1)-associated diseases is the infiltration of HTLV-1-infected T ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.11.8522-8530.1997
更新日期:1997-11-01 00:00:00
abstract::The Rel/NF-kappa B family of proteins includes several interacting cellular transcription factors and the v-Rel oncoprotein of the avian Rev-T retrovirus. We report the isolation of a chicken cDNA for the NF-kappa B p52 precursor protein p100. Full-length p100 only weakly binds DNA in vitro; removal of the ankyrin-lik...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.12.7612-7617.1993
更新日期:1993-12-01 00:00:00
abstract::Noninfectious spikeless particles have been obtained from vesicular stomatitis virus (VSV, Indiana serotype) by bromelain or Pronase treatment. They lack the viral glycoprotein (G) but contain all the other viral components (RNA, lipid, and other structural proteins). Triton-solubilized VSV-Indiana glycoprotein prepar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.75-84.1975
更新日期:1975-07-01 00:00:00
abstract::Seasonal influenza epidemics recur due to antigenic drift of envelope glycoprotein antigens and immune evasion of circulating viruses. Additionally, antigenic shift can lead to influenza pandemics. Thus, a universal vaccine that protects against multiple influenza virus strains could alleviate the continuing impact of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00150-11
更新日期:2011-05-01 00:00:00
abstract::Large polypeptides of the Bacillus anthracis protective antigen (PA) were inserted into an influenza A virus hemagglutinin glycoprotein (HA), and the chimeric proteins were functionally characterized and incorporated into infectious influenza viruses. PA domain 1', the region responsible for binding to the other toxin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.15.10003-10012.2005
更新日期:2005-08-01 00:00:00
abstract::SERINC3 (serine incorporator 3) and SERINC5 are recently identified host cell inhibitors of HIV-1 particle infectivity that are counteracted by the viral pathogenesis factor Nef. Here we confirm that HIV-1 Nef, but not HIV-1 Vpu, antagonizes the particle infectivity restriction of SERINC5. SERINC5 antagonism occurred ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01246-16
更新日期:2016-11-14 00:00:00
abstract::Human papillomaviruses (HPVs) infect keratinocytes of skin and mucosa. Homeostasis of these constantly renewing, stratified epithelia is maintained by balanced keratinocyte proliferation and terminal differentiation. Instructions from the extracellular matrix engaging integrins strongly regulate these keratinocyte fun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10738-10746.2004
更新日期:2004-10-01 00:00:00
abstract::The human antibody response to flavivirus infection is dominantly directed against a cross-reactive epitope on the fusion loop of domain II (DII-FL) of the envelope (E) protein. Although antibodies against this epitope fail to recognize fully mature West Nile virus (WNV) virions and accordingly neutralize infection po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05859-11
更新日期:2011-11-01 00:00:00
abstract::The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.31.3.584-589.1979
更新日期:1979-09-01 00:00:00
abstract::Enzymatic markers and electron microscopy were utilized to determine the cellular origin of the membrane types isolated from type 2 dengue virus-infected BHK cells by discontinuous sucrose gradient centrifugation. The results showed an apparent separation of plasma membrane, smooth and rough endoplasmic reticulum with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1017-1026.1975
更新日期:1975-10-01 00:00:00
abstract::The recent identification of coreceptors that mediate efficient entry of human immunodeficiency virus type 1 (HIV-1) suggests new therapeutic and preventive strategies. We analyzed simian immunodeficiency virus (SIV) entry cofactors to investigate whether the macaque SIV model can be used as an experimental model to e...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.9.6509-6516.1997
更新日期:1997-09-01 00:00:00
abstract::Coxsackievirus B3 (CVB3) is known to infect stem cells in the neonatal central nervous system. Here, we evaluated the effects of CVB3 infection on the major source and repository of stem cells, the bone marrow (BM). Viral genome was detectable in BM within 24 h of infection, and productive infection of BM cells was ev...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03004-12
更新日期:2013-03-01 00:00:00
abstract::The degree of genetic variability in the hypervariable region 1 of hepatitis C virus (HCV) was analyzed by cloning and sequencing HCV genomes obtained in paired samples of serum, liver tissue, and peripheral blood mononuclear cells (PBMC) from four chronic hepatitis C patients. Genetic variability in serum was higher ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.2.1640-1646.1998
更新日期:1998-02-01 00:00:00
abstract::Synthesis of the vesicular stomatitis virus membrane matrix protein and the glycoprotein is inhibited to a greater extent than the synthesis of the nucleocapsid protein, the nonstructural protein, and the large protein when the rate of peptide chain initiation is reduced by exposure of vesicular stomatitis virus-infec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.1.283-286.1976
更新日期:1975-01-01 00:00:00
abstract::Examination of the interaction between human immunodeficiency virus (HIV) regulatory gene products and the host immune system is fundamental to understanding the pathogenesis of HIV and could reveal possible targets for therapeutic intervention in the treatment of AIDS. The HIV Tat gene is a potential candidate for th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.12.7622-7629.1995
更新日期:1995-12-01 00:00:00
abstract::We have shown previously that processing of the Sindbis virus envelope protein precursor PE2 to envelope protein E2 is not required for virus maturation in cultured vertebrate fibroblast cells and that unprocessed PE2 can be incorporated into infectious virus in place of E2 (J. F. Presley and D. T. Brown, J. Virol. 63...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.1905-1909.1991
更新日期:1991-04-01 00:00:00
abstract::A lysate from hypotonically swollen polyoma-infected BALB/3T3 cells incorporated labeled deoxynucleotide triphosphates into both viral and cellular DNAs. The incorporation was stimulated by the presence of ATP, deoxynucleotide triphosphates, thiols, and magnesium ions. Strong inhibition of incorporation was observed w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.1.125-139.1974
更新日期:1974-01-01 00:00:00
abstract::Natural killer (NK) cells serve as a crucial first-line defense against tumors and virus-infected cells. We previously showed that lysis of influenza virus (IV)-infected cells is mediated by the interaction between the NK receptor, NKp46, and the IV hemagglutinin (HA) type 1 expressed by the infected cells. This inter...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01815-09
更新日期:2010-04-01 00:00:00
abstract::Scavenger receptor class B type I (SR-BI) is an essential receptor for hepatitis C virus (HCV) and a cell surface high-density-lipoprotein (HDL) receptor. The mechanism of SR-BI-mediated HCV entry, however, is not clearly understood, and the specific protein determinants required for the recognition of the virus envel...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02199-08
更新日期:2010-01-01 00:00:00
abstract::Rotavirus plus-strand RNAs not only direct protein synthesis but also serve as templates for the synthesis of the segmented double-stranded RNA (dsRNA) genome. In this study, we identified short-interfering RNAs (siRNAs) for viral genes 5, 8, and 9 that suppressed the expression of NSP1, a nonessential protein; NSP2, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.14.7763-7774.2004
更新日期:2004-07-01 00:00:00
abstract::The human immunodeficiency virus type 1 Vpr protein is both packaged into virions and efficiently localized to the nucleus. In this report, we show that a significant fraction of Vpr also accumulates in the cytoplasm of virus-producing cells. Although Vpr shuttles between the nucleus and the cytoplasm, studies with an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.75.17.8348-8352.2001
更新日期:2001-09-01 00:00:00
abstract::YY1 is a zinc finger transcription factor which acts as either a repressor or an activator dependent on the promoter context. YY1 is a potent activator of the genuine human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) in HeLa cells, which are known for high-level expression of the HPV-18 early gene...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.1.1-12.1995
更新日期:1995-01-01 00:00:00
abstract::Equine rhinitis A virus (ERAV) has recently been classified as an aphthovirus, a genus otherwise comprised of the different serotypes of Foot-and-mouth disease virus (FMDV). FMDV initiates translation via a type II internal ribosomal entry site (IRES) and utilizes two in-frame AUG codons to produce the leader proteina...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.24.11708-11716.2000
更新日期:2000-12-01 00:00:00