Abstract:
:Yellow fever virus (YFV), a member of the Flavivirus genus, has a plus-sense RNA genome encoding a single polyprotein. Viral protein NS3 includes a protease and a helicase that are essential to virus replication and to RNA capping. The 1.8-A crystal structure of the helicase region of the YFV NS3 protein includes residues 187 to 623. Two familiar helicase domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates and RNA triphosphate. The third, C-terminal domain has a unique structure and is proposed to function in RNA and protein recognition. The organization of the three domains indicates that cleavage of the viral polyprotein NS3-NS4A junction occurs in trans.
journal_name
J Viroljournal_title
Journal of virologyauthors
Wu J,Bera AK,Kuhn RJ,Smith JLdoi
10.1128/JVI.79.16.10268-10277.2005subject
Has Abstractpub_date
2005-08-01 00:00:00pages
10268-77issue
16eissn
0022-538Xissn
1098-5514pii
79/16/10268journal_volume
79pub_type
杂志文章abstract::Measles virus (MV) is the type species of the Morbillivirus genus and its RNA-dependent RNA polymerase complex is comprised of two viral polypeptides, the large (L) and the phospho- (P) proteins. Sequence alignments of morbillivirus L polymerases have demonstrated the existence of three well-conserved domains (D1, D2,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.14.7322-7328.2002
更新日期:2002-07-01 00:00:00
abstract::Clink, a 20-kDa protein of faba bean necrotic yellows virus, a single-stranded DNA plant virus, interacts with pRB family members and a SKP1 homologue from Medicago sativa. An LxCxE motif and an F-box of Clink mediate the interactions with the respective proteins. The capacity of Clink to bind pRB correlates with its ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.7.2967-2972.2000
更新日期:2000-04-01 00:00:00
abstract::Rhesus rhadinovirus (RRV) is a gammaherpesvirus closely related to Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic virus linked to the development of Kaposi's sarcoma and several other lymphoproliferative diseases, including primary effusion lymphoma and multicentric Castleman's disease. RRV naturally inf...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01429-10
更新日期:2010-11-01 00:00:00
abstract::The herpes simplex virus (HSV) heterodimer gE/gI and another membrane protein, US9, which has neuron-specific effects, promote the anterograde transport of virus particles in neuronal axons. Deletion of both HSV gE and US9 blocks the assembly of enveloped particles in the neuronal cytoplasm, which explains why HSV vir...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01113-20
更新日期:2020-11-09 00:00:00
abstract::We have previously shown for the paramyxovirus simian virus 5 (SV5) that a functional promoter for RNA replication requires proper spacing between two discontinuous elements: a 19-base segment at the 3' terminus (conserved region I [CRI]) and an 18-base internal region (CRII) that is contained within the coding region...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.8.3993-3998.2001
更新日期:2001-04-01 00:00:00
abstract::We previously reported that nucleolin, a representative nucleolar marker, interacts with nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) through two independent regions of NS5B, amino acids 208 to 214 and 500 to 506. We also showed that truncated nucleolin that harbors the NS5B-binding region inhibited the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3332-3340.2006
更新日期:2006-04-01 00:00:00
abstract::To analyze proteolytic processing of foamy (spuma) retroviruses, two mutations were generated in the presumed active-site triplet Asp-Ser-Gly in the predicted proteinase (PR) region of the human foamy virus (HSRV). The mutations changed either the presumed catalytic aspartic acid residue to a catalytically incompetent...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7264-7268.1995
更新日期:1995-11-01 00:00:00
abstract::Varicella-zoster virus (VZV) DNA was prepared from nucleocapsids and from enveloped virions of a laboratory strain (Ellen) and directly from the vesicle fluids of patients with zoster infections. VZV Ellen nucleocapsid DNA was cleaved with 11 different restriction endonucleases and electrophoresed in agarose gels. The...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.40.2.516-525.1981
更新日期:1981-11-01 00:00:00
abstract::The pathogenesis of Epstein-Barr virus (EBV) infection, including development of lymphomas and carcinomas, is dependent on the ability of the virus to transit from latency to the lytic phase. This conversion, and ultimately disease development, depends on the molecular switch protein, ZEBRA, a viral bZIP transcription...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00724-19
更新日期:2019-06-28 00:00:00
abstract::DDX21 regulates the biogenesis of rRNA and transcription of ribonucleoprotein genes. Recently, it has been reported that DDX21 regulates the growth of some RNA viruses through various mechanisms, such as inhibiting viral genome replication, suppressing virion assembly and release, and modulating antiviral immune respo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01222-19
更新日期:2019-11-26 00:00:00
abstract::H9N2 influenza viruses are panzootic in domestic poultry in Eurasia and since 1999 have caused transient infections in humans and pigs. To investigate the zoonotic potential of H9N2 viruses, we studied the evolution of the viruses in live-poultry markets in Hong Kong in 2003. H9N2 was the most prevalent influenza viru...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.16.8609-8614.2004
更新日期:2004-08-01 00:00:00
abstract::The E1 helicase of papillomaviruses is required for replication of the viral double-stranded DNA genome, in conjunction with cellular factors. DNA replication is initiated at the viral origin by the assembly of E1 monomers into oligomeric complexes that have unwinding activity. In vivo, this process is catalyzed by th...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.9.5178-5191.2003
更新日期:2003-05-01 00:00:00
abstract::Gene mapping experiments show that Fis-1, a preferred integration region for Friend murine leukemia virus in hematopoietic neoplasms, is extremely closely linked to Int-2, a preferred integration region for mouse mammary tumor virus in mammary carcinomas. Studies at the RNA and DNA level prove that these loci are dist...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.60.3.1156-1158.1986
更新日期:1986-12-01 00:00:00
abstract::The herpes simplex virus type 1 latency-associated transcript (LAT) inhibits apoptosis. We demonstrate here that LAT influences the accumulation of the Bcl-x(L) transcript versus the Bcl-x(S) transcript in Neuro-2A cells. Bcl-x(L) encodes an antiapoptotic protein, whereas Bcl-x(S) encodes a proapoptotic protein. Promo...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10714-10718.2003
更新日期:2003-10-01 00:00:00
abstract::Recently, we demonstrated that inverted repeat sequences inserted into first-generation adenovirus (Ad) vector genomes mediate precise genomic rearrangements resulting in vector genomes devoid of all viral genes that are efficiently packaged into functional Ad capsids. As a specific application of this finding, we gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.11.9314-9324.1999
更新日期:1999-11-01 00:00:00
abstract::Ribonucleic acid extracted from the virions of two human rhinoviruses is infective and is similar in size to poliovirus ribonucleic acid. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.7.2.278-280.1971
更新日期:1971-02-01 00:00:00
abstract::Previous studies with a limited number of strains have indicated that there are two genotypes of yellow fever (YF) virus in Africa, one in west Africa and the other in east and central Africa. We have examined the prM/M and a portion of the E protein for a panel of 38 wild strains of YF virus from Africa representing ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.15.6999-7008.2001
更新日期:2001-08-01 00:00:00
abstract::We previously conducted a phase I/II study using arterial infusions of ONYX-015 (dl1520), a replication-selective adenoviral vector, with E1b deleted, for patients with metastatic colorectal cancer. No dose-limiting toxicities occurred, but >90% of the patients experienced fever. The effects of temperature on the repl...
journal_title:Journal of virology
pub_type: 临床试验,杂志文章
doi:10.1128/JVI.79.1.581-591.2005
更新日期:2005-01-01 00:00:00
abstract::The putative role of nitric oxide in the neuropathogenesis of Borna disease was investigated by determining changes in the expression of inducible nitric oxide synthase (iNOS) mRNA and constitutively expressed NOS (cNOS) mRNA in brains of Borna disease virus (BDV)-infected rats. iNOS mRNA was not detected in normal ra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.10.5786-5791.1993
更新日期:1993-10-01 00:00:00
abstract::Cytotoxic T lymphocytes (CTLs) play an important role in the immune response against respiratory syncytial virus (RSV) infection. The cell surface molecule lymphocyte function-associated antigen 1 (LFA-1) is an important contributor to CTL activation, CTL-mediated direct cell lysis, and lymphocyte migration. In an att...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.78.6.3014-3023.2004
更新日期:2004-03-01 00:00:00
abstract:UNLABELLED:Many murine leukemia viruses (MLVs) are partially resistant to restriction by mouse APOBEC3 (mA3) and essentially fully resistant to induction of G-to-A mutations by mA3. In contrast, Vif-deficient HIV-1 (ΔVif HIV-1) is profoundly restricted by mA3, and the restriction includes high levels of G-to-A mutation...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03456-13
更新日期:2014-04-01 00:00:00
abstract::The foot-and-mouth disease virus (FMDV) capsid is highly acid labile, but introduction of amino acid replacements, including an N17D change in VP1, can increase its acid resistance. Using mutant VP1 N17D as a starting point, we isolated a virus with higher acid resistance carrying an additional replacement, VP2 H145Y,...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03222-13
更新日期:2014-03-01 00:00:00
abstract::The nef gene contributes to the replication of primate lentiviruses by altering the trafficking of cellular proteins involved in adaptive immunity (class I and II major histocompatibility complex [MHC]) and viral transmission (CD4 and DC-SIGN). A conserved acidic leucine-based sequence (E(160)xxxLL) within human immun...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.4.1837-1849.2006
更新日期:2006-02-01 00:00:00
abstract::Interferon (IFN)-stimulated gene factor 2 (ISGF2) plays a role in transcription of the beta IFN (IFN-beta) gene and IFN-stimulated genes (ISGs) and may function as a central mediator of cytokine responses. Constitutive ISGF2 transgene expression resulted in substantial resistance to three RNA virus families. This phen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.7.4470-4478.1992
更新日期:1992-07-01 00:00:00
abstract::The polyomavirus proteins VP1, VP2, and VP3 move from their cytoplasmic site of synthesis into the nucleus, where virus assembly occurs. To identify cellular or viral components which might control this process, we determined the distribution of VP1, VP2, and VP3 in a soluble fraction, a cytoplasmic cytoskeleton fract...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.2.516-525.1987
更新日期:1987-02-01 00:00:00
abstract::Different tombusviruses were able to support the replication of either homologous or heterologous defective interfering (DI) RNAs, and those infected plants usually developed typical attenuated symptoms. However, in some helper virus-DI RNA combinations the inoculated plants were necrotized, although they contained a ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.7.6251-6256.1998
更新日期:1998-07-01 00:00:00
abstract::The vaccinia virus H5 gene encodes a 22.3-kDa phosphoprotein that is expressed during both the early and late phases of viral gene expression. It is a major component of virosomes and has been implicated in viral transcription and, as a substrate of the B1 kinase, may participate in genome replication. To enable a gen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.5.2393-2405.2000
更新日期:2000-03-01 00:00:00
abstract::The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known clea...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4459-4463.1989
更新日期:1989-10-01 00:00:00
abstract::Viral protein R (Vpr) of human immunodeficiency virus type 1 has potent karyophilic properties, but details of the mechanism by which it enters the nucleus remain to be clarified. We reported previously that two regions, located between residues 17 and 34 (alphaH1) and between residues 46 and 74 (alphaH2), are indispe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.6.3557-3564.2005
更新日期:2005-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) transforms B lymphocytes through the expression of the latent viral proteins EBNA and latent membrane protein (LMP). Recently, it has become apparent that microRNAs (miRNAs) also contribute to EBV's oncogenic properties; recombinant EBVs that lack the BHRF1 miRNA cluster display a reduced abil...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05100-11
更新日期:2011-10-01 00:00:00