Abstract:
:The herpes simplex virus (HSV) heterodimer gE/gI and another membrane protein, US9, which has neuron-specific effects, promote the anterograde transport of virus particles in neuronal axons. Deletion of both HSV gE and US9 blocks the assembly of enveloped particles in the neuronal cytoplasm, which explains why HSV virions do not enter axons. Cytoplasmic envelopment depends upon interactions between viral membrane proteins and tegument proteins that encrust capsids. We report that tegument protein UL16 is unstable, i.e., rapidly degraded, in neurons infected with a gE-/US9- double mutant. Immunoprecipitation experiments with lysates of HSV-infected neurons showed that UL16 and three other tegument proteins, namely, VP22, UL11, and UL21, bound either to gE or gI. All four of these tegument proteins were also pulled down with US9. In neurons transfected with tegument proteins and gE/gI or US9, there was good evidence that VP22 and UL16 bound directly to US9 and gE/gI. However, there were lower quantities of these tegument proteins that coprecipitated with gE/gI and US9 from transfected cells than those of infected cells. This apparently relates to a matrix of several different tegument proteins formed in infected cells that bind to gE/gI and US9. In cells transfected with individual tegument proteins, this matrix is less prevalent. Similarly, coprecipitation of gE/gI and US9 was observed in HSV-infected cells but not in transfected cells, which argued against direct US9-gE/gI interactions. These studies suggest that gE/gI and US9 binding to these tegument proteins has neuron-specific effects on virus HSV assembly, a process required for axonal transport of enveloped particles.IMPORTANCE Herpes simplex viruses 1 and 2 and varicella-zoster virus cause significant morbidity and mortality. One basic property of these viruses is the capacity to establish latency in the sensory neurons and to reactivate from latency and then cause disease in peripheral tissues, such as skin and mucosal epithelia. The transport of nascent HSV particles from neuron cell bodies into axons and along axons to axon tips in the periphery is an important component of this reactivation and reinfection. Two HSV membrane proteins, gE/gI and US9, play an essential role in these processes. Our studies help elucidate how HSV gE/gI and US9 promote the assembly of virus particles and sorting of these virions into neuronal axons.
journal_name
J Viroljournal_title
Journal of virologyauthors
DuRaine G,Wisner TW,Johnson DCdoi
10.1128/JVI.01113-20subject
Has Abstractpub_date
2020-11-09 00:00:00issue
23eissn
0022-538Xissn
1098-5514pii
JVI.01113-20journal_volume
94pub_type
杂志文章abstract::Transient transfection assays indicated that A2L is one of three vaccinia virus intermediate genes that are required for the transcriptional transactivation of viral late genes. We have expressed the A2L open reading frame in Escherichia coli and shown by blotting experiments that the 26-kDa protein binds zinc, a prop...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1993-10-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of virology
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更新日期:1974-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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更新日期:1983-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1996-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:Journal of virology
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journal_title:Journal of virology
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更新日期:2014-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.4.2347-2354.1994
更新日期:1994-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2011-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3889-3892.1995
更新日期:1995-06-01 00:00:00
abstract::Koala retrovirus (KoRV) is of an interest to virologists due to its currently active endogenization into the koala (Phascolarctos cinereus) genome. Although KoRV has frequently been isolated in wild and captive koala populations, its pathogenesis and transmission remain to be fully characterized, and most previous res...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2020-05-18 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.10.6763-6766.1994
更新日期:1994-10-01 00:00:00
abstract:UNLABELLED:Baculovirus DNAs are synthesized and inserted into preformed capsids to form nucleocapsids at a site in the infected cell nucleus, termed the virogenic stroma. Nucleocapsid assembly ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) requires the major capsid protein VP39 and nine minor capsid pro...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2016-03-28 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.21.3.1128-1139.1977
更新日期:1977-03-01 00:00:00
abstract::The novel low-pathogenic avian influenza A H7N9 viruses (LPAI H7N9 viruses) have been a threat to public health since their emergence in 2013 because of the high rates of mortality and morbidity that they cause. Recently, highly pathogenic variants of these avian influenza A H7N9 viruses (HPAI H7N9 viruses) have emerg...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01277-17
更新日期:2017-11-14 00:00:00
abstract::Circulating interferon production, induced by Newcastle disease virus, is about seven times higher in C(57) Black mice than i Balb/c/Gif mice. A Mendelian analysis was carried out and circulating interferon production was measured in reciprocal F(1) hybrids, in the F(2) generation, in progeny of backcrosses of F(1) hy...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.3.5.506-512.1969
更新日期:1969-05-01 00:00:00