Abstract:
:Two defective RNAs (designated D RNA 3 alpha and D RNA 3 beta) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-length cDNA clone from which biologically active transcripts can be produced in vitro has been constructed for D RNA 3 beta. This transcript can be replicated in tobacco plants infected with subgroup I and II cucumber mosaic cucumovirus strains and with peanut stunt cucumovirus. Translation of D RNA 3 beta in vitro produced a 20-kDa peptide, which was consistent with the predicted coding capacity of the deleted 3a open reading frame. D RNA 3 beta was also associated with polyribosomes isolated from infected tobacco plants. The presence of the D RNAs had no apparent effect upon helper virus yield or symptom production.
journal_name
J Viroljournal_title
Journal of virologyauthors
Graves MV,Roossinck MJdoi
10.1128/JVI.69.8.4746-4751.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
4746-51issue
8eissn
0022-538Xissn
1098-5514journal_volume
69pub_type
杂志文章abstract:UNLABELLED:The immunoglobulin superfamily protein receptors for poliovirus, human rhinovirus, and coxsackievirus B (CVB) serve to bind the viruses to target cells and to facilitate the release of the virus genome by catalyzing the transition from the mature infectious virus to the A-particle uncoating intermediate. Rec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01790-14
更新日期:2014-10-01 00:00:00
abstract::The ability of the 3A protein of coxsackievirus B (CVB) to inhibit protein secretion was investigated for this study. Here we show that the ectopic expression of CVB 3A blocked the transport of both the glycoprotein of vesicular stomatitis virus, a membrane-bound secretory marker, and the alpha-1 protease inhibitor, a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.8.5163-5173.2005
更新日期:2005-04-01 00:00:00
abstract::In 2014 and 2015, the United States experienced an unprecedented outbreak of Eurasian clade 2.3.4.4 H5 highly pathogenic avian influenza (HPAI) virus. Initial cases affected mainly wild birds and mixed backyard poultry species, while later outbreaks affected mostly commercial chickens and turkeys. The pathogenesis, tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00960-17
更新日期:2017-10-13 00:00:00
abstract::An unusual human retrovirus was isolated from two patients with persistent generalized lymphadenopathy who originate from West-Central Africa and are currently residing in Belgium. Although the virus shared a number of the same biological and morphological properties as human immunodeficiency retrovirus type 1 (HIV-1)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.3.1207-1216.1990
更新日期:1990-03-01 00:00:00
abstract::Human cytomegalovirus (CMV) is the leading cause of prenatal viral infection. Affected infants may suffer intrauterine growth retardation and serious neurologic impairment. Analysis of spontaneously aborted conceptuses shows that CMV infects the placenta before the embryo or fetus. In the human hemochorial placenta, m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.15.6808-6820.2000
更新日期:2000-08-01 00:00:00
abstract::In the immunocompetent host, primary cytomegalovirus (CMV) infection is resolved by the immune response without causing overt disease. The viral genome, however, is not cleared but is maintained in a latent state that entails a risk of virus recurrence and consequent organ disease. By using murine CMV as a model, we h...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1797-1804.1998
更新日期:1998-03-01 00:00:00
abstract::We previously reported that a recombinant pantothenate auxotroph of Mycobacterium bovis BCG expressing human immunodeficiency virus type 1 (HIV-1) subtype C Gag (rBCGpan-Gag) efficiently primes the mouse immune system for a boost with a recombinant modified vaccinia virus Ankara (rMVA) vaccine. In this study, we furth...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03178-12
更新日期:2013-05-01 00:00:00
abstract::The enhancer unit present in the retrovirus equine infectious anemia virus (EIAV) was previously shown to contain binding sites for proteins belonging to MDBP, PEA2, AP-1, and ets families. The EIAV ets motif matches the consensus sequence for both PEA3- and PU.1-binding sites. Here, we show by gel shift analysis that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.7.3885-3890.1993
更新日期:1993-07-01 00:00:00
abstract::Varicella-zoster virus (VZV) glycoprotein I (gI) is dispensable in cell culture; the SCIDhu model of VZV pathogenesis was used to determine whether gI is necessary in vivo. The parental and repaired viruses grew in human skin and thymus/liver implants, but the gI deletion mutant was not infectious. Thus, gI is essenti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8468-8471.2002
更新日期:2002-08-01 00:00:00
abstract::Polymorphonuclear leukocytes (PMN) were studied for their ability to mediate cytotoxicity against varicella-zoster virus (VZV)-infected and uninfected human fibroblasts in 51Cr release assays. PMN were capable of mediating antibody-dependent cellular cytotoxicity (ADCC) against VZV-infected targets. Maximal ADCC was o...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.51.1.110-116.1984
更新日期:1984-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) isolates derived directly from clinical samples are usually unable to grow in cytokine-independent continuous cell lines, thus hindering the study of their biological features and their sensitivity to humoral and cellular protective immunity. To overcome these limitations, w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.6.3712-3720.1995
更新日期:1995-06-01 00:00:00
abstract::A chimeric porcine circovirus (PCV1-2) with the capsid gene of pathogenic PCV2 cloned into the genomic backbone of nonpathogenic PCV1 is attenuated in pigs but elicits protective immunity against PCV2. In this study, short epitope tags were inserted into the C terminus of the capsid protein of the chimeric PCV1-2 vacc...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02294-10
更新日期:2011-05-01 00:00:00
abstract::Amphiphilic fatty acid derivatives having a 14- to 20-carbon cis-unsaturated alkyl chain were found to be potent inhibitors of the entry process of the lipid-containing bacteriophage PR4. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.413-416.1979
更新日期:1979-01-01 00:00:00
abstract:UNLABELLED:Transmission of chronic wasting disease (CWD) between cervids is influenced by the primary structure of the host cellular prion protein (PrP(C)). In white-tailed deer, PRNP alleles encode the polymorphisms Q95 G96 (wild type [wt]), Q95 S96 (referred to as the S96 allele), and H95 G96 (referred to as the H95 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02010-15
更新日期:2015-12-01 00:00:00
abstract::Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phen...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00189-11
更新日期:2011-06-01 00:00:00
abstract::GP64, the major envelope glycoprotein of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) budded virion, is important for host cell receptor binding and mediates low-pH-triggered membrane fusion during entry by endocytosis. In the current study, we examined the functional role of the AcMNPV GP64 tr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02104-07
更新日期:2008-04-01 00:00:00
abstract::T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1155-1159.1976
更新日期:1976-06-01 00:00:00
abstract::Many viruses have evolved mechanisms to evade the repression of translation mediated by protein kinase R (PKR). In the case of murine cytomegalovirus (MCMV), the protein products of two essential genes, m142 and m143, bind to double-stranded RNA (dsRNA) and block phosphorylation of PKR and eukaryotic initiation factor...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01484-08
更新日期:2009-02-01 00:00:00
abstract::Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The ps...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.19.10249-10257.2004
更新日期:2004-10-01 00:00:00
abstract::Viral hemorrhagic septicemia virus (VHSV) infections cause high losses in cultured rainbow trout in Europe. Attempts to produce a recombinant vaccine based on the transmembrane glycoprotein (G protein) have indicated that proper folding is important for the antigenicity and immunogenicity of the protein. The present s...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.12.10189-10196.1998
更新日期:1998-12-01 00:00:00
abstract::Human papillomavirus type 1 (HPV1) E4 protein is associated with cytoplasmic and nuclear inclusions in productively infected keratinocytes. Here we have used transient expression of HPV1 E4 (also known as E1E4) protein in keratinocytes to reproduce formation of E4 inclusions. Immunofluorescence analysis showed that pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.1.673-684.2003
更新日期:2003-01-01 00:00:00
abstract::Heating oncornavirus RNAs at temperatures insufficient for complete denaturation results in forms migrating between the native form (vRNA) and the completely denatured form (vRNA) after gel electrophoresis. Intermediate forms from Rous sarcoma virus or murine leukemia virus were isolated after heating of vRNA's at 58 ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.19.3.810-819.1976
更新日期:1976-09-01 00:00:00
abstract::Phosphonoformate (foscarnet) is a pyrophosphate (PP(i)) analogue and a potent inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT), acting through the PP(i) binding site on the enzyme. HIV-1 RT can unblock a chain-terminated DNA primer by phosphorolytic transfer of the terminal residue t...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.11.6127-6137.2003
更新日期:2003-06-01 00:00:00
abstract::Nonenveloped gastrointestinal viruses, such as human rotavirus, can exit infected cells from the apical surface without cell lysis. The mechanism of such nonlytic exit is poorly understood. The nonenveloped Orsay virus is an RNA virus infecting the intestine cells of the nematode Caenorhabditis elegans Dye staining re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00745-18
更新日期:2018-06-29 00:00:00
abstract::Baculoviruses, although they infect insects in nature, can transduce a wide variety of mammalian cells and are therefore promising gene therapy vectors. However, baculovirus transduction into many mammalian cells is very inefficient, and the limiting stages and factors remain unknown. An important finding is that a sh...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00709-19
更新日期:2019-07-17 00:00:00
abstract::The VP7 and VP4 genes of seven antigenic mutants of simian rotavirus SA11 4fM (serotype 3) selected after 39 passages in the presence of SA11 4fM hyperimmune antiserum, were sequenced. Nucleotide sequence analysis indicated the following. (i) Twice as many amino acid substitutions occurred in the VP7 protein than in V...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.9.4534-4539.1990
更新日期:1990-09-01 00:00:00
abstract::The genus β human papillomavirus (HPV) type 8 is associated with nonmelanoma skin cancer in patients with epidermodysplasia verruciformis, and evidence for its protumorigenic potential in the general population increases. To date, strategies to suppress genus β HPV infections are limited. Interferon regulatory factors...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00998-10
更新日期:2011-01-01 00:00:00
abstract::Retrovirus assembly involves a complex series of events in which a large number of proteins must be targeted to a point on the plasma membrane where immature viruses bud from the cell. Gag polyproteins of most retroviruses assemble an immature capsid on the cytoplasmic side of the plasma membrane during the budding pr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.2.784-795.2000
更新日期:2000-01-01 00:00:00
abstract::Viruses cause about 15% of the cancers that are still the leading causes of human mortality. The discovery of viral oncogenes has enhanced our understanding of viral oncogenesis. However, the underlying molecular mechanisms of virus-induced cancers are complex and require further investigation. The present study has a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01392-10
更新日期:2011-01-01 00:00:00
abstract::Widespread distribution of highly pathogenic avian H5N1 influenza viruses in domesticated and wild birds continues to pose a threat to public health, as interspecies transmission of virus has resulted in increasing numbers of human disease cases. Although the pathogenic mechanism(s) of H5N1 influenza viruses has not b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02144-08
更新日期:2009-06-01 00:00:00