Abstract:
:Passive administration of porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing antibodies (NAbs) can effectively protect pigs against PRRSV infection. However, after PRRSV infection, pigs typically develop a weak and deferred NAb response. One major reason for such a meager NAb response is the phenomenon of glycan shielding involving GP5, a major glycoprotein carrying one major neutralizing epitope. We describe here a type II PRRSV field isolate (PRRSV-01) that is highly susceptible to neutralization and induces an atypically rapid, robust NAb response in vivo. Sequence analysis shows that PRRSV-01 lacks two N-glycosylation sites, normally present in wild-type (wt) PRRSV strains, in two of its envelope glycoproteins, one in GP3 (position 131) and the other in GP5 (position 51). To determine the influence of these missing N-glycosylation sites on the distinct neutralization phenotype of PRRSV-01, a chimeric virus (FL01) was generated by replacing the structural genes of type II PRRSV strain FL12 cDNA infectious clone with those from PRRSV-01. N-glycosylation sites were reintroduced into GP3 and GP5 of FL01, separately or in combination, by site-directed mutagenesis. Reintroduction of the N-glycosylation site in either GP3 or GP5 allowed recovery of in vivo and in vitro glycan shielding capacity, with an additive effect when these sites were reintroduced into both glycoproteins simultaneously. Although the loss of these glycosylation sites has seemingly occurred naturally (presumably by passage through cell cultures), PRRSV-01 virus quickly regains these glycosylation sites through replication in vivo, suggesting that a strong selective pressure is exerted at these sites. Collectively, our data demonstrate the involvement of an N-glycan moiety located in GP3 in glycan shield interference.
journal_name
J Viroljournal_title
Journal of virologyauthors
Vu HL,Kwon B,Yoon KJ,Laegreid WW,Pattnaik AK,Osorio FAdoi
10.1128/JVI.00189-11subject
Has Abstractpub_date
2011-06-01 00:00:00pages
5555-64issue
11eissn
0022-538Xissn
1098-5514pii
JVI.00189-11journal_volume
85pub_type
杂志文章abstract::The bovine papillomavirus type 1 (BPV-1) E7 oncoprotein is required for the full transformation activity of the virus. Although BPV-1 E7 by itself is not sufficient to induce cellular transformation, it enhances the abilities of the other BPV-1 oncogenes to induce anchorage independence. We have been exploring the mec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00422-07
更新日期:2007-09-01 00:00:00
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journal_title:Journal of virology
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pub_type: 杂志文章
doi:10.1128/JVI.60.2.782-786.1986
更新日期:1986-11-01 00:00:00
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journal_title:Journal of virology
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doi:10.1128/JVI.54.2.576-585.1985
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.41.2.709-709.1982
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journal_title:Journal of virology
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doi:10.1128/JVI.2.5.401-408.1968
更新日期:1968-05-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.12.4.841-846.1973
更新日期:1973-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.71.3.2500-2504.1997
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.2.596-603.1976
更新日期:1976-05-01 00:00:00
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of virology
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更新日期:2018-03-14 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.6.2191-2195.1988
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更新日期:1969-11-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1972-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.7.2182-2191.1987
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.1.250-260.1979
更新日期:1979-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-12-23 00:00:00