Abstract:
:Cucumber mosaic virus (CMV) is a tripartite RNA virus that can support the replication of satellite RNAs, small molecular parasites of the virus. Satellite RNAs can have a dramatic effect on the helper virus and the host plant in a manner specific to the helper, satellite, and host. Previously, we showed that the Sny-CMV strain is not able to support the replication of the WL1 satellite RNA in zucchini squash and that this phenotype maps to RNA 1. In the present study, we use recombinant cDNA clones of Fny- and Sny-CMV RNA 1 and a site-directed mutant of Fny-CMV RNA 1 to demonstrate that the inability to support WL1 satellite RNA maps to a single amino acid at residue 978 in the 1a protein, proximal to the helicase domain VI. Support of satellite RNA in whole plants and in protoplasts of zucchini squash is analyzed.
journal_name
J Viroljournal_title
Journal of virologyauthors
Roossinck MJ,Kaplan I,Palukaitis Pdoi
10.1128/JVI.71.1.608-612.1997subject
Has Abstractpub_date
1997-01-01 00:00:00pages
608-12issue
1eissn
0022-538Xissn
1098-5514journal_volume
71pub_type
杂志文章abstract::One-step growth determinations were performed with five strains of rotavirus in HepG2, a cell line derived from human liver. Three virus strains (SA11-C13, SA11-C14, and RRV) replicated in HepG2 cells and attained yields 10- to 100-fold above input titers. Two virus strains (B223 and SA11-4F) failed to replicate above...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::Three distinct strains of murine rotavirus were adapted to growth in cell culture. These strains are genetically related but not identical; they are serotypically heterogeneous. The cultivatable strains were substantially more infectious (approximately 10(6)-fold) for suckling mice than heterologous simian rotaviruses...
journal_title:Journal of virology
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.5.6.663-671.1970
更新日期:1970-06-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.5.4524-4529.1999
更新日期:1999-05-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.8.5100-5107.1994
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JVI.49.3.652-657.1984
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pub_type: 杂志文章
doi:10.1128/jvi.76.20.10245-10255.2002
更新日期:2002-10-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2015-04-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.62.3.1055-1056.1988
更新日期:1988-03-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.13.2.340-346.1974
更新日期:1974-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02014-12
更新日期:2013-02-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7341-7348.1998
更新日期:1998-09-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.19.9068-9076.2001
更新日期:2001-10-01 00:00:00
abstract::Hepatitis C virus (HCV) nonstructural protein 5B (NS5B) is an RNA-dependent RNA polymerase (RdRp) that acts as a key player in the HCV replication complex. Understanding the interplay between the viral and cellular components of the HCV replication complex could provide new insight for prevention of the progression of...
journal_title:Journal of virology
pub_type: 杂志文章
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abstract::Measles virus (MV) interacts with cellular receptors on the surface of peripheral blood lymphocytes (PBL) which mediate virus binding and uptake. Simultaneously, the direct contact of the viral glycoproteins with the cell surface induces a negative signal blocking progression to the S phase of the cell cycle, resultin...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.10.4499-4505.2001
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-08-01 00:00:00
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journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.2.1049-1058.2003
更新日期:2003-01-01 00:00:00
abstract::Transmission of human immunodeficiency virus type 1 (HIV-1) occurs primarily via the mucosal route, suggesting that HIV-1 vaccines may need to elicit mucosal immune responses. Here, we investigated the immunogenicity and relative efficacy of systemic immunization with two human ALVAC-HIV-1 recombinant vaccines express...
journal_title:Journal of virology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
abstract::The dengue type 4 virus (DEN4) genome contains a 384-nucleotide (nt) 3' noncoding sequence in which the last 81 nt, predicted to form a secondary structure, are thought to be essential for virus replication. Immediately upstream of the secondary structure, short RNA sequences that are conserved among mosquito-borne fl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3930-3937.1996
更新日期:1996-06-01 00:00:00
abstract::After herpes simplex virus infection of hamster kidney cells there is an induction of nucleoside phosphotransferase activity which can utilize AMP as phosphate donor. The activity is immunologically specific for the infected cell and is induced concomitantly with the virus-coded pyrimidine deoxynucleoside kinase activ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.17.3.1056-1059.1976
更新日期:1976-03-01 00:00:00
abstract::Previous studies identified a common site (Sfpi-1) for proviral integration in immortalized Friend erythroleukemias. cDNAs corresponding to a 1.5-kb Sfpi-1 mRNA were isolated and sequenced. These were larger than an independently isolated Sfpi-1 cDNA described by researchers from another laboratory, and they contained...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.1.464-467.1991
更新日期:1991-01-01 00:00:00
abstract::Translation of poliovirus RNA occurs by the binding of ribosomes to an internal segment of RNA sequence within the 5' untranslated region of the viral RNA. This region is predicted to consist of six domains (I to VI) that possess complex secondary and tertiary structures. Domain IV is a large region in which alteratio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.7.4381-4389.1995
更新日期:1995-07-01 00:00:00
abstract::Three monkeypox virus (MPV) antibody-secreting murine monoclones were characterized as being of the immunoglobulin G1 isotype, gave a 4+ reaction in the indirect fluorescent-antibody test, gave a positive reaction in the enzyme immunoassay, and did not neutralize MPV. These monoclonal antibodies were determined by the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.1.290-292.1984
更新日期:1984-10-01 00:00:00
abstract::Antigens detected by the complement-fixation (CF) test were prepared from BHK-21 cells infected with Pichinde virus. The preparations contained two antigens demonstrable by immunodiffusion. The antigen present in abundance was heat stable, Pronase resistant, and had a molecular weight of 20,000 to 30,000 as estimated ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.22.1.175-186.1977
更新日期:1977-04-01 00:00:00
abstract::Equine infectious anemia virus (EIAV) provides a natural model system by which immunological control of lentivirus infections may be studied. To date, no detailed study addressing in parallel both the humoral and cellular immune responses induced in horses upon infection by EIAV has been conducted. Therefore, we initi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3840-3852.1997
更新日期:1997-05-01 00:00:00
abstract::Intercellular adhesion molecule 1 (ICAM-1) mediates binding and entry of major group human rhinoviruses (HRVs). Whereas the entry pathway of minor group HRVs has been studied in detail and is comparatively well understood, the pathway taken by major group HRVs is largely unknown. Use of immunofluorescence microscopy, ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01693-09
更新日期:2010-04-01 00:00:00